Bio 2010_autotrophic Nutrition Notes

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    The Chemistry of

    Photosynthesis

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    Learners Outcomes

    Describe the full process of photosynthesis with

    reference to the full equation for photosynthesis.

    Describe the light-dependent reaction of photosynthesisas involving only water and light, and producing oxygengas and the energy molecules ATP and NADPH (in

    simple terms).

    Describe the light-independent reaction ofphotosynthesis as involving the products of the light-

    dependent reaction (ATP and NADPH) converting

    carbon dioxide to glucose (in simple terms).

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    Learners Outcome

    Explain that ATP is an energy carrier in cellsbecause it has high-energy phosphate bonds that

    release energy when broken.

    Show that the rate of photosynthesis is dependent

    on various factors

    Explain the effect of changes in temperature, lightintensity, wavelength, carbon dioxide and oxygen

    concentration on the rate of photosynthesis.

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    Where does

    photosynthesishappen?

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    !"#$%%&&&'()*#+,-./0'1,2%#!,3,45/%1!+,-,#+643'!32+

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    !"#$%%&&&'()*#+,-./0'1,2%#!,3,45/%1!+,-,#+643'!32+89:;

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    The chloroplast is the main site for photosynthesis

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    Thylakoids- where light reaction of

    photosynthesis occurs

    Stroma- where light independent

    reaction occurs

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    EFG

    =)C 6/C D+H) +.0!3 6D4,-D)C D5 1!+,-,#!5++

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    Photosynthesis occurs in 2 stages

    Light dependent stage

    Light independent stage

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    Light dependent reaction

    !12 H2O + 12NADP + 18ADP + 18Pi+ light and

    chlorophyll

    +

    6 O2+ 12 NADPH + 18 ATP

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    Light reaction

    !Occurs in thylakoids

    !Chlorophyll molecule captures light energy

    !Excited electron causes water to split into

    hydrogen and oxygen

    !High energy molecules - ATPand NADPH

    produced

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    P700

    P680

    Photon hits, excited

    electrons picked up by

    reaction center

    PS II splits water into !O2

    and 2 electrons. These

    electrons replaces those

    going to PSI.

    ATP produced as protons

    move thru ATP synthetase

    complexes

    Meanwhile, photons hit

    P700 complex and excited

    electrons are picked up by

    ETC of PSI.

    PSI ETC produces NADPH

    and H+

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    What is ATP?

    Adenosine Triphosphate

    High energy molecule

    Energy found in the bondsbetween the phosphate ionsand adenosine

    Bond broken = energyreleased

    Bond formed = energy

    required

    2

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    The Calvin Cycle (Dark Reactions)

    !A.k.a light-independent reactions

    !Occurs in stroma.

    ! 12NADPH + 18ATP + 6CO2=> Glucose +

    12NADP++ 18ADP + 18Pi +O2

    !Fixes CO2to produce glucose.

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    Factors Affecting Photosynthesis

    !Carbon dioxide concentration

    !Water

    !Light intensity

    !Chlorophyll levels

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    Graph of Rate of Photosynthesis against Light

    Intensity

    !As light intensity increases, rate

    of photosynthesis increases.

    ! There is a limit to the maximum

    rate (saturation point).

    !At the saturation point, the rate

    of photosynthesis cannot

    increase due to the presence of

    a limiting factor.

    !

    Only by increasing the limitingfactor can the rate increase.

    Rate of

    Photosynthesis

    Light Intensity

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    Observe how a change in the CO2concentration

    affects the graphs

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    Practice Time!

    !Draw a graph of rate of photosynthesis against

    light intensity.

    !Label this graph as one showing the graph attemperature T.

    !Now draw on the same axes another graph, but

    this time at temperature T+10.

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    Photosynthesis and Temperature

    !Did it look like this?

    !Note the circled regions of A and B.

    !Do you know the significance of the trendsshown at A and B?

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    Factors affecting

    photosynthesis

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    Uses of Glucose

    Triose phosphates and glucose are used as a material to

    build most other biomolecules (these are products from

    photosynthesis)

    Disaccharides

    Cellulose

    Starch

    Amino acids

    Lipids

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