BG iMatrix-511 & Now Available BG iMatrix-511 Silk · β1 γ1 α5 β1 E8 α5 E8 γ1 E8 Ideal for...
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BG iMatrix-511 is a recombinant laminin 511 E8 fragment of the natural laminin protein, which is a crucial cell adhesion protein that interacts with relevant cell signaling receptors to enhance cell viability and functionality. The E8 fragment retains the full integrin binding activity of laminin and was found to adhere more strongly to ES/iPS cells than the full-length protein. Now Available BG iMatrix-511 & BG iMatrix-511 Silk —S— —S— Laminin-511 (Intact) [800 kDa] Laminin-511E8 Fragment [150 kDa] β1 γ1 α5 β1 E8 α5 E8 γ1 E8 Ideal for Xeno-Free, Animal- Free, and Feeder-Free Cell Culture Research Enables single cell passaging Greater adhesion than Matrigel and the full-length laminin Use Pre-Mix method to save time Coat twice as many wells with the same amount of solution using the Pre-Mix method Human iPS cells adhere more strongly to Laminin-511E8 fragment than to the “Laminin-511 (Intact)” Single human ES cells attach rapidly to Laminin-511E8 fragment and proliferate Volume production of ES/iPS cell culture using Laminin-511E8 fragment Miyazaki et al., Nature Commun. 3: 1236, 2012 (Supplementary information) * The figure is partially modified. Miyazaki et al., Nature Commun. 3: 1236, 2012 Japanese patent application No.2009-234583/PCTJP2010-067618/WO2011-043405 Miyazaki et al., Nature Commun. 3: 1236, 2012 * The figure is partially modified. culture period (day) cell numbers, relative Laminin-511E8 fragment Matrigel (The main component is Laminin-111) Laminin-511E8 fragment Matrigel (The main component is Laminin-111) coating concentration (μg/cm 2 ) Dose-response adhesion curves of iPS cells on ECMs absorbance, relative
Transcript of BG iMatrix-511 & Now Available BG iMatrix-511 Silk · β1 γ1 α5 β1 E8 α5 E8 γ1 E8 Ideal for...
BG iMatrix-511 is a recombinant laminin 511 E8 fragment of the natural laminin protein, which is a crucial cell adhesion protein that interacts with relevant cell signaling receptors to enhance cell viability and functionality. The E8 fragment retains the full integrin binding activity of laminin and was found to adhere more strongly to ES/iPS cells than the full-length protein.
Now AvailableBG iMatrix-511 &BG iMatrix-511 Silk
—S— —S—
Laminin-511 (Intact)[800 kDa]
Laminin-511E8 Fragment[150 kDa]
β1 γ1
α5β1 E8 α5 E8 γ1 E8
Ideal for Xeno-Free, Animal- Free, and Feeder-Free Cell Culture Research
Enables single cell passaging
Greater adhesion than Matrigel and the full-length laminin
Use Pre-Mix method to save time
Coat twice as many wells with the same amount of solution using the Pre-Mix method
Human iPS cells adhere more strongly to Laminin-511E8 fragment than to
the “Laminin-511 (Intact)”
Single human ES cells attach rapidly to Laminin-511E8 fragment and
proliferate
Volume production of ES/iPS cell culture using Laminin-511E8 fragment
Miyazaki et al., Nature Commun. 3: 1236, 2012 (Supplementary information)* The figure is partially modified.
Miyazaki et al., Nature Commun. 3: 1236, 2012 Japanese patent application No.2009-234583/PCTJP2010-067618/WO2011-043405
Miyazaki et al., Nature Commun. 3: 1236, 2012 * The figure is partially modified.
culture period (day)
cell
num
bers
, rel
ativ
e Laminin-511E8fragment
Matrigel(The main component is Laminin-111)
Laminin-511E8fragment
Matrigel(The main component is Laminin-111)
coating concentration (μg/cm2)
Dose-response adhesion curves of iPS cells on ECMs
abso
rban
ce, r
elat
ive
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ECM Pre-mix Method (Without Pre-coating)
BG iMatrix-511
BG iMatrix-511 Silk
T A B L E O F P R O D U C T S :
Name Cat# Size Source Price
BG iMatrix-511 Silk exhibits equal integrin-binding activity as iMatrix-511 produced by CHO-S cells
RL511-350
RL511-1050
RL511S-350
RL511S-1050
350UG
1050UG
350UG
1050UG
CHO-S Cell
CHO-S Cell
Transgenic Silkworm Cocoon
Transgenic Silkworm Cocoon
$355
$890
$225
$560
For Research Use Only. Not For Use in Diagnostic Procedures
ECM Pre-coated Method
STEP 1 Dilute the BG iMatrix-511 or the BG iMatrix-511 Silk solution with sterile PBS(-).
Coat dishes with 0.5μg/cm2.(Optimal coating concentration depends on each cell line, from 0.1 to 1.5μg/cm2)
Incubate for one of the following:• 1 h at 37OC• 3 h at room temperature• Over night at 4OC.
Remove remaining fluid from the coated surface, and plate the cells at 1.0 to 2.0 x 104 cells/cm2.
STEP 2
STEP 3
STEP 4
Add and mix BG iMatrix-511 or BG iMatrix-511 Silk solution to the cell suspension at passing.(Recomended concentration: 0.25μg/cm2)
Plate the mixture of cell and BG iMatrix-511 solution at 1.0 to 2.0 x 104 cells/cm2.
STEP 1
STEP 2
2 STEPSONLY!
COST EFFECTIVE
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