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How Bacteria Talk to Each OtherBonnie Bassler, Princeton University

KITP Public Lecture, Jan 19, 2011

Bacteria

Humans?

Humans?

Humans?

Humans?

A Potpourri of Notorious Bacteria

Food PoisoningToxic ShockLyme Disease

CholeraUlcers

Vibrio fischeri Communication

Low Cell Density High Cell Density

No Light Bioluminescence

Vibrio fischeri Communication

Low Cell Density High Cell Density

No Light Bioluminescence

Vibrio fischeri Communication

Signal ReceptorProtein

BioluminescenceSignal Producing

Protein(Light)

Bacterial Quorum Sensing

Signal ReceptorProtein

Group BehaviorSignal Producing

ProteinGenes

Intra-Species Communication

Serratia liquefaciensliquefaciens

Vibrio harveyi

Vibrio fischeriVibrio fischeri

Agrobacterium gtumefaciens

O OO O

P d NH

Pseudomonas aeruginosa

Intra-Species Communication

V. fischeri Bioluminescence

P.aeruginosa Virulence Factors, Biofilms

E. carotovora Virulence Factors, Antibiotics

Bacterial Communities

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Vibrio harveyi Quorum Sensing

System 1 System 2

Signal 1 Signal 2

Light

Vibrio harveyi Quorum Sensing

System 1

Signal 1

Light

Vibrio harveyi Quorum Sensing

System 2

Signal 2

Light

Bacteria With LuxS

Actinobacillus actinomycetemcomitansActinobacillus pleuropneumoniaeA ti b ill i

Neisseria gonorrhoeae Neisseria meningitidisP t ll lt id

(A sample of 299/534 genomes sequenced)

Actinobacillus suisBacillus anthracisBacillus haloduransBacillus subtilisB li b d f i

Pasteurella multocidaPasteurella trehalosiPorphyromonas gingivalisProteus mirabilisS l ll t hiBorrelia burgdorferi

Campylobacter jejuniClostridium acetobutylicumClostridium difficileCl t idi f i

Salmonella paratyphiSalmonella typhiSalmonella typhimuriumShewanella putrefaciensShi ll fl iClostridium perfringens

Deinococcus radioduransEscherichia coli MG1655Escherichia coli O157:H7E t f li

Shigella flexneriStaphylococcus aureusStaphylococcus epidermidisStreptococcus gordoniiSt t tEnterococcus faecalis

Haemophilus influenzaeHelicobacter pyloriKlebsiella pneumoniaeL t l ti

Streptococcus mutansStreptococcus pneumoniaeStreptococcus pyogenesVibrio choleraeVib i h i Lactococcus lactis

Leuconostoc oenosListeria monocytogenesMannheimia haemolytica

Vibrio harveyi Vibrio parahaemolyticusVibrio vulnificusYersinia pestis

System 2-Controlled TraitsBacillus anthracis GrowthBacillus subtilis DevelopmentBorrelia burgdorferi VirulenceCampylobacter jejuni Motility ToxinsCampylobacter jejuni Motility, ToxinsClostridium difficile Virulence, ToxinsClostridium perfringens Virulence, ToxinsEscherichia coli EHEC O157:H7 Virulence, Type III SecretionEscherichia coli EPEC Motility Type III SecretionEscherichia coli EPEC Motility, Type III SecretionHaemophilus influenzae Virulence, Cell invasionHelicobacter pylori Motility, BiofilmsKlebsiella pneumoniae BiofilmsListeria monocytogenes BiofilmsListeria monocytogenes BiofilmsNeisseria meningitidis Virulence, BacteremiaPorphyromonas gingivalis Virulence, ProteasesSalmonella typhi BiofilmsSalmonella typhimurium AI-2 transport BiofilmsSalmonella typhimurium AI-2 transport, BiofilmsSerratia marcescens Virulence, Antibiotic productionShigella flexneri VirB (virulence factor)Staphylococcus epidermis Virulence, BiofilmsStreptococcus gordonii Mixed-species biofilmsStreptococcus gordonii Mixed-species biofilmsStreptococcus pneumoniae Virulence, Persistence, CompetenceStreptococcus pyogenes Virulence factors, HemolysinVibrio cholerae Virulence, Toxins, Biofilms

A Universal Communication Molecule

Multi-Lingual Bacteria

Intra-Species Inter-Species

Signal 1 Signal 2

Group BehaviorGenesGenes

A New Approach to Antibiotics

Chemistry

Vibrio harveyi Quorum Sensing

System 2

Signal 2

LightNo Light

Vibrio harveyi Quorum Sensing

System 1

Signal 1

Light

Vibrio harveyi Quorum Sensing

System 2

Signal 2

No Light

Vibrio harveyi Quorum Sensing

System 1

Signal 1

Light

A New Approach to Antibiotics

DEADALIVE

Virulent Bacteria

Quorum Sensing In BacteriaBacteria talk to each other

Bacteria are multi-cellular

D l t t i t i d /i

Bacteria can distinguish self from other

Develop strategies to impede/improve quorum sensing

Natural anti- and pro-quorum sensingstrategies already existstrategies already exist

Thank you!