Batch Processing - NFSTC Projects · 2009-12-08 · Batch Processing increasing lab effectiveness...
Transcript of Batch Processing - NFSTC Projects · 2009-12-08 · Batch Processing increasing lab effectiveness...
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Batch Processing increasing lab effectiveness
Robin Freeman, M.S. Harris County Medical
Examiner’s Office
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BATCH METHOD
As businesses (laboratories) grow and demand increases, the work flow process is often changed to a “batch method”. Batch methods require that a group of items move through a process together, a stage at a time.
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Advantages The batch method can be an advantage when administered appropriately. It is cheaper to complete a number of items in one go because instruments can be used more effectively, and the staff can specialize in that task. There are advantages of employees concentrating their skills.
. They become more expert at their tasks, which will in turn increase productivity (output per employee).
Employees are more familiar with processes and so can find ways of improving them.
Builds team work among sections and staff meetings become more interactive.
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Disadvantages
Batch methods can result in the build up of significant “work in progress” (i.e. completed batches waiting for their turn to be worked on in the next operation).
Managers must monitor workflow and foresee bottlenecks in the process. The batch method is not a hands-off process. Batching requires very careful planning to implement in the beginning.
Quality checks/verification steps must be put in place to monitor work product.
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Preventative measures.
Gowning required prior to entering an evidence exam area
Monthly Swipe test
Separate sectional Quality group
In-House databases
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Evidence Examination
Items are individually examined. Areas of interest are identified, tested and documented.
– Presumptive tests – Confirmatory tests
Samples are prepared for DNA testing and placed on appropriate extraction log.
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DNA extraction
Extraction logs Differentials Low yield Regular Knowns
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Multi-Step DNA Test Process Purification
Quantization Amplification
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Multi-Step DNA Test Process Analysis
Interpretation Reporting
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Planning and Implementation
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Rape Kits
Extraction
Differential
Organic Chile Siegen
Other Evidence
Extraction
Known Organic Chelex Qiagen
Quantitation
Report FAX/ Email
PCR Set-up Amplification
Run
Profile Analysis Interpret Report
Tech Rev Admin
Rev FAX/ Email
Sexual Assault case Homicide case /Property crimes
Neg Neg
pos pos
Process Flow Chart for the DNA Unit
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SCHEDULE: Mon- Fri: 7-3:30 ART/DDH/LGS/
ACS/KDW LIG/MAD/DOJ
(7-3:30) or (8-5:00) AT-TB
Lab Lock
Kits (1) _____
Assigned Projects
Homicide (1) Burglary Property
Cut Only (1)
Kit –(Other
Evidence) (1)
Other Evidence
3 Minimum Overtime
Case Management
Ext. Robot Verifications
Manual PCR/ Run
Analyze
Report Writing Review
August 24 4th fl. LGS LIG 5th fl. LG
ACS LIG
Bleach______ ART 5:00 Rotation LGS
DDH______ LGS MAD______ JBS 3:30-5
DOJ
________ AT TB
ART KDW
JBS/SP Bleach/
Autoclave: Robot
ZP DCY
WP AB Float
Run: RF
Paper: CS/DG/
KM
RUC RPT =MLP//MG
TR=RF/JP/JW AR =MP/ CS/
DG/KM
August 25 4th fl. LGS LIG 5th fl. LG
ACS
Bleach______ ART 5:00 Rotation LGS
DDH______ LGS MAD______ JBS 3:30-5
DOJ
________ AT TB
ART KDW LIG
JBS/SP Bleach/
Autoclave: Robot
WP TET
DCY ZP Float AB Float
Run: RF
Paper: CS/DG/
KM
RUC RPT =MLP//MG
TR=RF/JP/JW AR =MP/ CS/
DG/KM
August 26 4th fl. LGS LIG 5th fl. LG
ACS
Bleach______ ART 5:00 Rotation LGS
DDH______ LGS MAD______ JBS 3:30-5
DOJ
________ AT TB
ART KDW LIG
JBS/SP Bleach/
Autoclave: Robot
WP TET
DCY ZP Float AB Float
Run: RF
Paper: CS/DG/
KM
RUC TR=RF/JP/JWMLP/MG
AR =MP/ CS/DG/KM
August 27 4th fl. LGS LIG 5h fl. LG
ACS
Bleach______ ART 5:00 Rotation LGS
DDH______ LGS MAD______ JBS 3:30-5
DOJ
________ AT TB
ART KDW LIG
JBS/SP Bleach/
Autoclave: Robot
WP TET
DCY ZP Float AB Float
Run: RF
Paper: CS/DG/
KM
RUC TR=RF/JP/JWMLP/MG
AR =MP/ CS/DG/KM
August 28 4th fl. LGS LIG 5h fl. LG
ACS
Bleach______ ART 5:00 Rotation LGS
DDH______ LGS MAD______ JBS 3:30-5
DOJ
________ AT TB
ART KDW LIG
JBS/SP Bleach/
Autoclave: Robot
WP TET
DCY ZP Float AB Float
Run: RF
Paper: CS/DG/
KM
RUC TR=RF/JP/JWMLP/MG
AR =MP/ CS/DG/KM
Organization of staff using Rotation schedules
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Quantitation CE Load/ Analysis
Reporting
Amplification
Extraction
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1. Samples are counted to determine the reactions needed. 2. Kit lot information is stored in the template itself. 3. By selecting the correct kit, all other lot information is automatically entered. 4. Master mix volumes are calculated based on reaction count. After Importing the Quant data: 5. The required dilutions are automatically calculated. 6. Reagent Blanks are also automatically recognized.
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All calculations are performed automatically using the Quant and Dilution data (from the previous worksheets).
8. Kit lot information is stored in the template itself
9. By selecting the correct kit, all other lot information is automatically entered, including the quant value of the positive control
7. macro in the template allows exported quant data from the ABI 7000 or 7500 to be imported into the template. This data will be used in amplification reaction calculations.
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10. Simple links populate the PCR Amplification Plate Grid
11. Selecting the instrument for injections alters the plate import worksheet that follows
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12. Sample names are automatically imported from the previous worksheet.
13. Sample type and panel are determined by the sample name.
14. Analysis method, size standard and results group are determined by the instrument chosen on the proceeding worksheet.
15. Instrument protocol is decided based on Quantification values.
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Case # Serology stats Serology reports
Productivity Index
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Productivity Index DNA Stats DNA Reports
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Monthly metrics
Tracked daily
Personal statistics
calculated in spreadsheet
by adding the number of
task completed
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Metrics Normalized
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Metrics Outlier, low PI
Outlier, High PI
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Staff improvement
Process improvement
no increase in staff number since 2007
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Process improvement
• September 2008-2009:
• ~50% Increased monthly output • ~85% Decreased backlog
• ~25% Increased CODIS monthly hits
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Process improvement
• Increased monthly output • DNA cases reported September 2008 = 130 • DNA cases reported September 2009 = 267
• Decreased backlog (>60 days)
• Backlog as of September 2008 = 1077 • Backlog as of September 2009 = 163
• Increased CODIS monthly hits • CODIS matches September 2008 = 21 • CODIS matches September 2009 = 86
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Thank You
Contact information: Robin Freeman, M.S. Forensic Biology-Interpretation Manager Harris County Medical Examiner’s Office (713) 796-6974 [email protected]
Dr. Roger Kahn Forensic Biology Director Harris County Medical Examiner’s Office (713) 796-6978 [email protected]