Basic Lab Safety WHMIS - University of Manitobabibeauel/safety/WHMIS_all.pdf · Basic Lab Safety &...

1 Title PageUniversity of ManitobaUniversity of Manitoba

Basic Lab SafetyBasic Lab Safety&&

WHMISWHMISRevised March 24, 2010Revised March 24, 2010

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Responsibilities for Safety at U of M ………………….. Slides 3-9Personal Safety, Emergency Contacts, Accident/IncidentReporting, Working Alone Policy, Fire Safety ………… Slides 13-26Chemical Safety program: Generic WHMIS …………... Slides 28-84Radiation and Biological Safety program descriptions … Slides 85-97General Lab Safety

Personal Protective Clothes and Safety Equipment … Slides 102-118Waste disposal ……………………………………… Slides 119-135Spill clean-up ……………………………………….. Slides 136-148Post Biological Exposure Protocol & Immunization.… Slides 149-153

Presentation OverviewPresentation Overview

Note- Grey boxes with white writing usually contain links to a related web-site

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Responsibilities for SafetyResponsibilities for Safety

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Manitoba’s Provincial Workplace Safety and Health Act (WS&H) is based on the concept of an:

INTERNAL RESPONSIBILITY SYSTEM

This means that everyone at the workplace be they employers, supervisors, workers, students, contractors or self employed persons, all share a responsibility for the health and safety of all persons at the workplace.


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The WS&H Act provides the following Four Basic Rights:Right to know about hazards (WHMIS)Right to refuse dangerous workRight to protection from discrimination re: safety andhealth mattersRight to participate in safety activities (through safety committees).

At the U of M every department MUST appoint a person to implement the WHMIS requirements. Know your departmental WHMIS coordinator.


U of M WHMIS Coordinator Duties

http://umanitoba.ca/admin/human_resources/ehso/media/whmiscoordduties.pdf

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EHSO

WHMISCoordinator/Safety

OfficerDesignated by

Department

President And

Vice Presidents

Deans, Directors and Department

Heads

Supervisor(Project Manager,

PrincipalInvestigator)

Laboratory WorkerTechnician,

PDF, Research Associate, Visiting

Scientists

Students

Responsibilities for SafetyResponsibilities for SafetyOverview

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At the University of Manitoba, the Department or its Principal Investigators and / or Supervisors are responsible to provide workers with:

- a safe work environment- adequate safety equipmentsafety training

Principal Investigator / Supervisors

U of M Health and Safety Policy

U of M Health and Safety Policies- Laboratory Safety Training Standard

http://www.umanitoba.ca/admin/governance/governing_documents/staff/551.htm

http://www.umanitoba.ca/admin/governance/governing_documents/operations/800.htm

http://www.umanitoba.ca/admin/governance/governing_documents/operations/800.htm

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University of Manitoba, employees and students are required to:

- follow Departmental and Principal Investigator’s established safety protocols

- use reasonable care to protect your safety and the safety of others

- bring safety concerns to the attention of the principalinvestigator or designated safety individual

Employees and Students

U of M Health and Safety Policy


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HAVE THE RIGHT TO REFUSE TO DO WORK THAT YOU CONSIDER TO BE UNSAFE

You must inform your PI and file an incident report with the department. Generic forms are available on the EHSO website. Concerns can also be addressed through your departmental safety committee representative.

Employees and Students

Provincial Workplace Safety & Health Act

http://web2.gov.mb.ca/laws/statutes/ccsm/w210e.php

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EHSO is responsible for the development and administration of environmental health and safety programs at the University of Manitoba.EHSO provides information, consultation, expertise, training, advice and authorization.

EHSO Website:U of M homepage > UM info > Search “safety office”

http://www.umanitoba.ca/admin/human_resources/ehso/

U of M Environmental Health & Safety Office (EHSO)

http://umanitoba.ca/admin/human_resources/ehso/

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Accident InvestigationAsbestos ManagementAudiometric TestingBiological SafetyChemical SafetyConfined Space EntryConstruction SafetyEmergency Planning & ResponseFire & Life SafetyGeneral SafetyHazardous Waste Management

Indoor Air QualityLaser SafetyOccupational HealthOffice Safety & ErgonomicsPersonal SafetyRadiation SafetySafety CommitteesTraffic SafetyTransportation of Dangerous GoodsWorkers Compensation

U of M Environmental U of M Environmental Health & Safety Office (EHSO)Health & Safety Office (EHSO)

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John Van Delden Director ……………………………………………….. 474-9290Pearl Novotny – Administrative Assistant …………………………………. 474-6633 Terry Neufeld – Office Assistant Office Assistant ………………………………………………………………………………………….. 474474--90319031Paul Richards – Fire Marshall / Facility Safety Coordinator …………… 474-9083Charlie Swan – Construction Safety Specialist Construction Safety Specialist ……..……………………………….... .... 474474--85768576Prabhat Goswami – Biological / Chem Safety Coordinator ………………. 474-8791Evelyn Froese – Assistant Bio/Chem Safety Coordinator …………………... 789-3477Steven Cole – Animal Care Occupational Health Specialist ……………….. 789-3359 Paul Houle – Environmental Management Coordinator …………………… 474-6316Joey Bellino – Assistant Environmental Management Coordinator …………474-6970Alison Reineke – Environmental Management Technologist ………………. 474-7970 Leona Page – Radiation Safety Coordinator ………………………………… 789-3613Eva Sailerova – Assistant Radiation Safety Coordinator …………………… 789-3359Alison Yarmill – Laboratory Safety Technologist………………………………..789-3654Judy Shields – Occupational Health Coordinator ……………………………474-6438Main Office Phone: 474-6633 Bannatyne Campus: T248/249 Old Basic Science Fort Garry Campus: 191 Frank Kennedy Centre

EHSO Location and StaffEHSO Location and Staff

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Personal SafetyPersonal SafetyEmergency Contacts Emergency Contacts Accident/Incident ReportingAccident/Incident ReportingWorking Alone PolicyWorking Alone PolicyFire SafetyFire Safety

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Provide 24/7 instant voice connection to Security ServicesOfficer will automatically be sent and arrive in under 2 minutesStrategically placed at both campuses

Code Blue Station

Personal SafetyPersonal Safety

Safewalk ProgramCall 474-9341For students, faculty, and staff at both campuses, a student patrol with specially marked security vests or a uniformed Security Services Officer will provide a safe walk to your car or other building at night.

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Other safety resourcesRed Emergency Buttons: are available in certain buildings and tunnels. Pushing the red button will alert Security Services that an emergency exists at that locationNew Red Call Boxes: are two-way communication boxes with Security Services.

Personal SafetyPersonal Safety

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At UofM dial 555 (not 911) from any 474-, 789-, 975- telephone exchange or 474-9341 from all other phonesor dial #555 from any Roger’s or MTS cell phoneAt HSC / CCMB dial 55 (not 911) from any 787 - telephone exchangeAll 55 or 555 calls go directly to Security Services who will send the appropriate response and also accompany outside emergency vehicles to the correct location

egeg. medical emergency, fire, assault, . medical emergency, fire, assault, violence, traffic accidentviolence, traffic accident

Emergency ContactsEmergency Contacts

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Lab Location: Rm 807-808 BMSBPI: Dr. Robert Safety 787-2121Eyewash/Safety Shower:

Hallway by Room 456Fire Extinguisher

Hallway by Room 123Fire / Medical Emergency 555UM EHSO (Environmental Health &

Safety Office) CHEMICAL / RADIATION / BIOHAZARD8:30am-4:30pm 474-6633 Outside Regular Hours 474-9341 (MICB -also call HSC security 74567)

Dept. WHMIS Coordinator:Mr. Martin Keener 787-2122

Dept. Radiation Coordinator:Dr. Isaac Aymhot 787-3333

MSDS Location: North bookcase Rm 807bUM EHSO Website:www.umanitoba.ca/campus/health_and_safety

Fire Wardens:Mr. Donny Flame 787-2133Dr. Johnny Nitro 787-2124

SAFETY INFO & FIRST AID KIT:Hallway outside Rm 808SPILL KIT Locations:Under sink Rm 807

Labs / Departments must have safety and emergency information provided to ALL employees. See example below.

Departmental Emergency Contactsand Safety Information

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Emergency ContactsWorkplace Hazard Information Placard (WHIP)

To meet the intent of the Manitoba Fire Code and the requirements of Workplace Safety and Health Act, all rooms and laboratories where controlled products are used, stored or handled must have a hazard warning placard posted at the entrance in order to provide hazard and emergency contact information. Prior to entering a room, you can use the information on the WHIP to help you make safe choices. The information provided on the WHIP is:•Building and Room Identification•Entrance Requirements•Recommended Personal Protective Equipment•Hazards in the Room•Emergency Contact Information •MSDS and First Aid Kit Locations

WHIP form

http://www.umanitoba.ca/admin/human_resources/ehso/geninfo/signage.html

http://www.umanitoba.ca/admin/human_resources/ehso/geninfo/signage.html

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Legislation requires that all accidents, incidents and near misses shall be investigated and a report completed. Incidents include the following:

personal injury (including needle sticks)occupational illnessfire/explosionproperty and equipment damageenvironmental damagenear miss incidents (those incidents that could have resulted in any of the above losses)

Accident/Incident ReportingAccident/Incident Reporting

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Staff and Students:Report the incident to your supervisor immediately: verbally or using the Employee’s Green Card-Notice of Injury Form available on EHSO web-site.If required, seek medical attention immediately. Make sure to tell your supervisor of the visit.

Additionally for staff only:Complete the Employee’s WCB Report. It can be faxed directly to WCB. Your supervisor must complete an Employer’s Report and fax it within 24 hrs to the EHSO if you consulted a health care professional with respect to your accident.Both forms and complete responsibilities can be found on EHSO web-site.

Procedures – Personal Injury


Notice of Injury Form

WCB Reporting forms

http://umanitoba.ca/admin/human_resources/ehso/media/GreenCardFormDec06.pdf

http://umanitoba.ca/admin/human_resources/ehso/occ_health_comp/aiwcb.html

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Accidents and incidents are to be investigated to:Meet legislative requirements.Identify the cause and relationships involved in the occurrence of accidents and incidents.Recommend changes to equipment, procedures, and any other matters that will ensure the health and safety of academic, support staff, students and visitors on campus.

Procedures – Personal Injury


Accident/Injury Reporting Procedure with A/I Short Form and Sample Report

COHS Accident Investigation Guide

WS&H Accident Investigation for Committees

http://www.umanitoba.ca/admin/human_resources/ehso/media/AccidentInvestigationCCOHS.pdf

http://umanitoba.ca/admin/human_resources/ehso/media/AIguideWSH.pdf

http://umanitoba.ca/admin/human_resources/ehso/media/AIguideWSH.pdf

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When a serious accident occurs at a workplace, the employer (UM) must notify Manitoba Labour-Workplace Safety and Health Division as quickly as possible.During regular hours: EHSO at 474-6633. If after hours: U of M Security at 555 or HSC at 55

Serious accidents include:a collapse or structural failure of a building, tower, crane, hoist or excavationa death or serious injuryuncontrolled spill of a hazardous materialexplosion, fire or flooding

Accident/Incident ReportingAccident/Incident ReportingProcedures- Serious Accidents and Injuries

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Serious injuries are defined as: AmputationLoss of sightInternal haemorrhageThird degree burnsUnconsciousness resulting from concussion,electrical contact, asphyxiationPoisoningCuts requiring hospitalisation or time off workAny injury resulting in paralysisAny other injury likely to endanger life or causepermanent disability


Procedures- Serious Accidents and Injuries

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Workplace Safety and Health Act recognizes that certain workplaces require staff to work alone and therefore requires that a plan is in place that is agreeable to both the employer and employee. The plan should be based on a realistic risk assessment of the hazards under the circumstances and include a written emergency response plan

Review your Department’s “Working Alone Policy”

Policies might include:Mandatory buddy system where staff and students must work in

pairs.Supervisor notification before starting work after hours. Restrictions to certain types of work.Restrictions to certain employees or employees with specific

training.Mandatory use of “Safewalk” service to get to your car.

Departmental Working Alone PolicyDepartmental Working Alone Policy

University of Manitoba: University Governance -Working Alone: Procedures



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Get to know the alarm bell scenarios in your building.

When you hear a fire alarm you should turn off all flames and gas sources and prepare to exit the building via the stairway.

If you need assistance proceed to nearest stairway and await the FIRE WARDEN

Contacts for departmental fire information / trainingand the fire wardens are posted at your Departmental Office.

Fire SafetyFire Safety

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Know Your Fire Alarm & Know Your Fire Alarm & Extinguisher LocationExtinguisher Location

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Chemical (WHMIS)

Radiation

Biological

Combined Lab Inspection Program (CLIP)

Lab Specific Safety ProgramsLab Specific Safety Programs

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WWorkplaceorkplaceHHazardousazardousMMaterialsaterialsIInformationnformationSSystemystem

WHMIS

Chemical Safety Program

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Canada wide programLegislated October 31, 1988 Provides Canadian workers with information on safe use of hazardous materials in their workplaceCombination of Federal and Provincial legislations

Federal legislation includes:sale and importation and defines labels and MSDS requirementsdefinition of six classes of hazards and the scientific criteria for inclusion in one or more of the hazard classes.

Provincial legislation includes:safe use, handling, storage and disposal

WHMIS: LegislationWHMIS: Legislation

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To reduce injury and illness from exposure to hazardous materials in the workplace

WHMIS: GoalWHMIS: Goal

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Education

Labels

Material Safety Data Sheets (MSDS)

WHMIS: Three Key ElementsWHMIS: Three Key Elements

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Definition:A “Controlled Product” is any substance or material which meets any of the criteria for inclusion in one or more of the six (6)WHMIS Hazard Classes as defined in the Federal Controlled Product Regulation.

Under WHMIS , there is no comprehensive list of controlled products but only a list of hazard criteria.

WHMIS: Controlled ProductsWHMIS: Controlled Products

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Risks:Physical hazard (120kg)

Explosive hazard(if heated,dropped or impacted)

Content hazard(could be flammable,poisonous)

Class A : Compressed GasesClass A : Compressed Gases

WHMIS Controlled Products: WHMIS Controlled Products: Hazard Classes & SymbolsHazard Classes & Symbols

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Precautions:Secure cylinder

Avoid heat & ignition sources

Transport & handle carefully



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Storage and Transportation:Secure cylinder

Store in cool ventilated area

Transport using specialized cart



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Examples:

CO2 tanks

N2 tanks

O2 tanks

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Class B : Flammable & CombustibleClass B : Flammable & Combustible


Six subdivisions:1. flammable gas2. flammable liquid3. combustible liquid4. flammable solid5. flammable aerosol6. reactive flammable material

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Risks:Fire hazard – will burn if ignited

Could ignite spontaneously(under adverse conditions)

Could ignite upon mixing with wateror other chemicals

Many are poisonous



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Precautions:Avoid contact with flames or otherignition sources

Avoid heat

Cap tightly for storage as vapoursare more flammable than liquid

Avoid inhalation and skin contact



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Storage and Transportation:Store in flammable storage cabinets(or specialized containers or intrinsically safe refrigerators if cold storage required)

Transport separate from oxidizing materials

Transport securely to prevent accidental spillage



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Examples:1. Flammable Gases - hydrogen, methane2. Flammable liquids [flash pt <37.8C]-

gasoline, acetone, ether, ethanol3. Combustible Liquids [flash pt >37.8C] -

kerosene, varsol4. Flammable Solids - magnesium metal,

aluminum dust5. Flammable Aerosols - aerosol containers6. Reactive Flammable Materials - phosphorus,

sodium metal

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Risks:Increase fire and explosion hazard of flammable and combustibles

Potentially explosive when mixed with flammables or organics

Most are corrosive and poisonous


Class C : Oxidizing MaterialClass C : Oxidizing Material

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Precautions:Wear protective clothing and avoidpersonal contact especially eyes

Store away from flammables and organics and avoid heating

Store in non-corroding containers –corks, rubber stoppers, glass, certain plastics may not be appropriate



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Storage and Transportation:Store away from flammables and organics

Store in appropriate containers

Transport separate from flammables




45 WHMIS Controlled Products: WHMIS Controlled Products: Hazard Classes & SymbolsHazard Classes & Symbols

Examples:Any oxygen source including:

O2 gasperoxidesnitrates / nitriteschlorates / chloriteshypochlorites (bleach)perchloratesdichromatespermanganatespersulfates



Class D : Poisonous and InfectiousClass D : Poisonous and Infectious

Division 1 – Materials causingImmediate and Serious Toxic Effects (acute)

Division 2 – Materials causing Other Toxic Effects (chronic, delayed)

Division 3 – Biohazardous InfectiousMaterial

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Division 1 Toxic: Acute and/or Serious

Risks:

Harmful or lethal at small amountsLD50 (Lethal Dose 50) <50mg / kg body wt

May be toxic not only if ingested butalso if inhaled or absorbed throughskin or eyes

Many acute toxic compounds act ascarcinogens at lower levels



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Division 2 – Toxic: Chronic and/or Delayed

Risks:

Systemic damage with low levelrepeated exposure

Damage could include:Permanent illness or deathBirth defects in pregnant womenActivation of cancer formationSensitization to allergies



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Division 3 – Biohazardous Infectious Material

Risks:

Systemic damage with accidental or prolonged exposure

Damage could include:Acquiring a disease or infectionActivation of cancer formationSensitization to allergies



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Precautions:Wear protective clothing including labcoat, correct gloves, eye protection and if necessary an approved respirator

If appropriate work in a fumehood or BSC

Avoid all personal exposure includingskin contact, inhalation, ingestion andinjection

Clean even minor spills carefully toavoid creating dust or vapor



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Precautions:Beware of aerosols particularly duringsonication and homogenization

If working with human or animal samples ensure that you are immunized against Hepatitis A/B or Tetanus/Diphtheria respectively

If working with human blood assume it is HIV and Hepatitis B&C positive



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Storage and Transportation:Store tightly capped in appropriate location

Assume exterior is contaminated, always handle with gloves





Division 1 Toxic: Acute and/or Immediate

Examples:All halogens (Br, Cl, F, I)Cyanides & nitriles (HCN)Heavy metals like arsenic,cadmiumHeavy metal salts like nickel acetateHydrogen Sulfide, Nitrogen dioxide



Division 2 Toxic: Chronic and/or Delayed

Examples:Heavy metals like mercury & leadSolvents like formaldehyde, benzene, carbon tetrachlorideReagents like acrylamide, ethidiumbromideNumerous carcinogens, mutagens and teratogens



Division 3 Biohazardous Infectious MaterialIncludes all Risk Group 2-4 organisms (Those organisms that can cause disease in humans-consult Health Canada’s “Laboratory Biosafety Guidelines”, more details later)

Examples:Human blood, tissue and body fluidsAnimal blood, tissue and body fluidsTissue culture cell linesExperimental bacterial, viral and yeast cultures

Health Canada’s “Laboratory Biosafety Guidelines”


http://www.phac-aspc.gc.ca/ols-bsl/lbg-ldmbl/index.html


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Risks:Will burn human tissues including skin, eyes, mouth, throat & lungs

Will corrode many lab related materials particularly metals

Fumes may be environmentally damaging


Class E : Corrosive MaterialClass E : Corrosive Material

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Precautions:Wear appropriate protective clothingIf possible work in the fume hoodAvoid personal contactAvoid inhaling vaporsCleanup spills immediatelyStore in non-corroding containers –corks, rubber stoppers, glass, certain plastics may not be appropriate



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Storage and Transportation:Store away from combustibles andorganics

Store in non-corrosive containers

Transport separate from flammables

Transport securely to preventaccidental spillage




Examples:Most oxidizing agentsStrong acids & basesGases like hydrogen fluoride, hydrogen chloride, chlorine,bromine, sulfur dioxide


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Risks:Can react violently under certainconditions including:

contact with water or other chemicalsif heated or shakenif allowed to polymerize

Can release poisonous vapors when mixed with other chemicals


Class F : Dangerously ReactiveClass F : Dangerously Reactive

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Precautions:Determine unstable conditions (MSDS) and avoid these conditions

Wear protective clothing, especiallyeye protection

Open slowly and carefully

Use in fume hood

Store tightly capped in chemical cabinet or fume hood



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Storage and Transportation:Store away from potential “trigger”chemicals

Store in chemical cabinet or fume hood

Transport securely to prevent accidental dropping, spillageand radical temperature changes



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Examples:Picric acid: unstable when desiccated Ether: unstable peroxidesPerchloric acid: highly unstable whendehydrated (concentration 85% and higher)1,3-butadiene: vigorous polymerizationAlkali cyanides: releases cyanide gas when mixed with acids

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Canadian Canadian vsvs US symbolsUS symbols


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Education

Labels



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Supplier Labels:1. Product Name2. Supplier Name3. WHMIS Symbol(s)4. MSDS Reference

5. Precautions [Sigma 64+]

6. First Aid Measures

7. Risk Phrase(s)[Sigma 68+]

Workplace Labels:

1. Product Name

2. MSDS Reference

3. Safe Handling Instructions

Types of labelsTypes of labels

WHMIS Labels

Waste Tag Labels:

1. Product Name

2. Concentration

3. Hazard

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Supplier label Supplier label

Product IdentifierSupplier IdentifierHazard symbol(s)Risk phrase(s)Precautionary measuresFirst aid measuresReference to the MSDS

WHMIS Labels

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Laboratory Supply House Laboratory Supply House -- Supplier labelSupplier label

WHMIS Labels

3) Symbol

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Must be present on:products decanted or transferred from

an original containerproduct where original label is lost or

becomes illegibleproducts produced and used at the

workplace

Methanol

x

x

x

xx

MethanolFlammable, poisonous, harmful vaporKeep away from heat, sparks, flamesAvoid contact with eyes and skin

WHMIS Labels

Print your own WHMIS Workplace Labels

University of ManitobaWorkplace Label

Product Identifier

________________________________________________________________________________________________________________________________

Hazard (s)check all that apply

FlammableOxidizerToxic/poisonousCorrosive

pH: __________Reactive/explosi

veSensitizer/allerge

n

BiohazardCarcinogenMutagenTeratogenSkin/eye/lung

irritantOther specify:

_______________

Safe Handling /Personal Protection

No inhalationNo ingestionNo skin/eye

contactNo chronic

exposure

GlovesGlasses/gogglesN-95 maskOrganic vapor

maskOther:_________

____

Name: Dept:

Phone #: Date:_________________

Refer to Material Safety Data Sheets for more info

http://umanitoba.ca/admin/human_resources/ehso/media/WHMIS_Label.doc

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WHMIS Labels

Waste Tags University of ManitobaWaste Tag

To be attached to waste container

Chemical Name(s) Conc.

1. ___________________________2. ___________________________3. ___________________________4. ___________________________

____%____%____%____%

Total volume: ___________

Hazard (s)check all that apply

FlammableCorrosive

pH: __________ToxicReactiveOther specify:______________

BiohazardousOxidizer

If biohazardous, has this material been sterilized?YesNo

NOT FOR USE WITH RADIOACTIVE WASTE

Generator information

Name:_______________ Dept: ________________

Phone #: Start Date:_____________

Print your own Hazardous Waste TagsThese tags are not intended for Radioactive Waste.

Must be present on All containers of hazardous waste generated in the lab if:

Container does not have a correct supplier labelWaste is a cocktail of different compatible chemicals

http://www.umanitoba.ca/admin/human_resources/ehso/media/HWasteLabels.pdf

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Education

Labels



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Provide detailed information about the controlled product. Must be provided by the supplier.Must be no more the three years old from the date the MSDS is produced or revised.If you have synthesized a product, you must prepare a MSDS.Risk Group 2 and higher biological agents also require an MSDS. It is against the law to use controlled products in the workplace without the presence of an MSDS.

WHMIS MSDS:Material Safety Data Sheet

CC INFO Web MSDS (accessible from UM only)

Health Canada Biological Agent MSDSs

http://ccinfoweb.ccohs.ca/

http://www.phac-aspc.gc.ca/msds-ftss/index.html

73 WHMIS MSDS:Comparison with other standards

ANSI / EU / ILO / ISO StandardsSection

1. Product and Company Identification2. Composition / Information on Ingredients3. Hazards Identification4. First Aid Measures5. Fire Fighting Measures6. Accidental Release Measures7. Handling and Storage8. Exposure Controls/Personal Protection9. Physical and Chemical Properties

10. Stability and Reactivity11. Toxicological Information12. Ecological Information13. Disposal Considerations14. Transport Information15. Regulatory Information16. Other Information

WHMISSection

1. Product Information2. Hazardous Ingredients3. Physical Data4. Fire and Explosion Hazard5. Reactivity Data6. Toxicological Properties7. Preventive Measures8. First Aid Measures9. Preparation Information

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WHMIS MSDS:Example (ANSI / EURO / ILO / ISO Standards)

M A T E R I A L S A F E T Y D A T A S H E E T

SECTION 1. - - - - - - - - - CHEMICAL IDENTIFICATION- - - - - - - - - -NAME: (+)-AMETHOPTERIN* CATALOG #: 06563SECTION 2. - - - - - COMPOSITION/INFORMATION ON INGREDIENTS - - - - - -CAS #: 59-05-2 MF: C20H22N8O5 EC NO: 200-413-8SYNONYMS: AMETHOPTERIN * (+)-AMETHOPTERIN * AMETHOPTERINE * SECTION 3. - - - - - - - - - - HAZARDS IDENTIFICATION - - - - - - - - -LABEL PRECAUTIONARY STATEMENTS TOXIC; MAY CAUSE HARM TO THEUNBORN CHILD; MAY CAUSE GENETIC DAMAGE; MAY IMPAIR FERTILITY; TOXIC IF SWALLOWED; IRRITATING TO EYES……TARGET ORGAN(S): BLOOD; BONE MARROW……SECTION 4. - - - - - - - - - - FIRST-AID MEASURES- - - - - - - - - - -IN CASE OF CONTACT, IMMEDIATELY FLUSH EYES OR SKIN WITH WATER.SECTION 5. - - - - - - - - - FIRE FIGHTING MEASURES - - - - - - - - - -EXTINGUISHING MEDIA; WATER SPRAY; CARBON DIOXIDE; DRY CHEMICAL POWDER; WEAR SELF-CONTAINED BREATHING APPARATUS AND PROTECTIVE CLOTHING

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SECTION 6. - - - - - - - - ACCIDENTAL RELEASE MEASURES- - - - - - - - -WEAR SELF-CONTAINED BREATHING APPARATUS, HEAVY RUBBER BOOTS RUBBER GLOVES. SWEEP UP, PLACE IN A BAG AND HOLD FOR WASTE DISPOSALSECTION 7. - - - - - - - - - - HANDLING AND STORAGE- - - - - - - - - - -REFER TO SECTION 8.SECTION 8. - - - - - - EXPOSURE CONTROLS/PERSONAL PROTECTION- - - - - -WEAR APPROPRIATE NIOSH/MSHA-APPROVED RESPIRATOR, CHEMICAL-RESISTANTGLOVES, SAFETY GOGGLES, OTHER PROTECTIVE CLOTHING.SAFETY SHOWER AND EYE BATH.USE ONLY IN A CHEMICAL FUME HOOD.DO NOT BREATHE DUST.DO NOT GET IN EYES, ON SKIN, ON CLOTHING.WASH THOROUGHLY AFTER HANDLING.KEEP TIGHTLY CLOSED.SECTION 9. - - - - - - - PHYSICAL AND CHEMICAL PROPERTIES - - - - - - -APPEARANCE AND ODOR SOLID.

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SECTION 10. - - - - - - - - -STABILITY AND REACTIVITY - - - - - - - - -INCOMPATIBILITIES: STRONG OXIDIZING AGENTS; STRONG ACIDSMAY DECOMPOSE ON EXPOSURE TO LIGHT.PROTECT FROM MOISTURE.HAZARDOUS COMBUSTION OR DECOMPOSITION PRODUCTSTOXIC FUMES OF: CARBON MONOXIDE, CARBON DIOXIDE;NITROGEN OXIDESSECTION 11. - - - - - - - - - TOXICOLOGICAL INFORMATION - - - - - - - -ACUTE EFFECTS; TOXIC IF SWALLOWED; MAY BE HARMFUL IF INHALED; MAY BE HARMFUL IF ABSORBED THROUGH THE SKIN; CAUSES EYE AND SKIN IRRITATION MATERIAL IS IRRITATING TO MUCOUS MEMBRANES AND UPPER RESPIRATORY TRACT; EXPOSURE CAN CAUSE: NAUSEA, DIZZINESS AND HEADACHEBLOOD EFFECTS: CHRONIC EFFECTS: MAY CAUSE CONGENITAL MALFORMATION IN THE FETUS. MAY ALTER GENETIC MATERIAL; MAY CAUSE REPRODUCTIVE DISORDERS.THIS PRODUCT IS OR CONTAINS A COMPONENT THAT IS NOT CLASSIFIABLE AS TO ITS CARCINOGENICITY BASED ON ITS IARC, ACGIH, NTP OR EPA CLASSIFICATION.

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TARGET ORGAN(S): BLOOD BONE MARROW LIVER G.I. SYSTEM KIDNEYS IMMUNE SYSTEM LUNGSTARGET ORGAN DATABRAIN AND COVERINGS (CHANGES IN CEREBRAL SPINAL FLUID)SPINAL CORD (OTHER DEGENERATIVE CHANGES)SENSE ORGANS AND SPECIAL SENSES (OLFACTION TUMORS)SENSE ORGANS AND SPECIAL SENSES (OTHER EYE EFFECTS)BEHAVIORAL (CONVULSIONS OR EFFECT ON SEIZURE THRESHOLD)VASCULAR (BP LOWERING NOT CHARACTERIZED IN AUTONOMIC SECTION)LUNGS, THORAX OR RESPIRATION (FIBROSIS, FOCAL)LUNGS, THORAX OR RESPIRATION (RESPIRATORY OBSTRUCTION)LUNGS, THORAX OR RESPIRATION (COUGH)LUNGS, THORAX OR RESPIRATION (DYSPNAE)LUNGS, THORAX OR RESPIRATION (CYANOSIS)LUNGS, THORAX OR RESPIRATION (OTHER CHANGES)LUNGS, THORAX OR RESPIRATION (TUMORS)GASTROINTESTINAL (NAUSEA OR VOMITING)Etc, etc, etc.

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SECTION 12. - - - - - - - - - ECOLOGICAL INFORMATION - - - - - - - - - -DATA NOT YET AVAILABLE.SECTION 13. - - - - - - - - - DISPOSAL CONSIDERATIONS - - - - - - - - -DISSOLVE OR MIX THE MATERIAL WITH A COMBUSTIBLE SOLVENT AND BURN IN A CHEMICAL INCINERATOR EQUIPPED WITH AN AFTERBURNER AND SCRUBBER. OBSERVE ALL FEDERAL, STATE AND LOCAL ENVIRONMENTAL REGULATIONS.SECTION 14. - - - - - - - - - - TRANSPORT INFORMATION - - - - - - - - -CONTACT FLUKA CHEMICAL COMPANY FOR TRANSPORTATION INFORMATION.SECTION 15. - - - - - - - - - REGULATORY INFORMATION - - - - - - - - - -EUROPEAN INFORMATION TOXIC R 61 MAY CAUSE HARM TO THE UNBORN CHILD.SECTION 16. - - - - - - - - - - OTHER INFORMATION- - - - - - - - - - - -THE ABOVE INFORMATION IS BELIEVED TO BE CORRECT BUT DOES NOT PURPORT TO BE ALL INCLUSIVE AND SHALL BE USED ONLY AS A GUIDE. SIGMA, ALDRICH,

79 WHMIS MSDS:Know the location of your MSDS collection

80

The U of M requires all responsible owners of chemicals to maintain an inventory of the controlled products in their workplace on the web-accessible EHS Assistant database.

To obtain secure access to the database PIs/responsible owners can submit a

U of M WebU of M Web--AccessibleAccessibleChemical Inventory DatabaseChemical Inventory Database

WHMIS: Chemical Inventories

Database access link

Lab Registration Form

http://www.umanitoba.ca/admin/human_resources/ehso/ehs_db/ehsassist.html

http://umanitoba.ca/admin/human_resources/ehso/media/New_Principal_Investigator_Lab_Registration080826.doc

81

All containers must be labeled and tightly capped.Maximum container size in labs is 4L.Avoid exposure to heat and direct sunlightCabinets used for storage should not have exposed plumbingor electrical fixtures.Store by compatibility not alphabetically.

Flammables Store in flammable storage cabinets (often yellow).Acids Store in corrosive resistant acid cabinet,[EXCEPT chromic, nitric and perchloric acids which should be stored separately and glacial acetic should be stored as a flammable].

Bases Store separately (ex: sodium hydroxide).

Organic Solvents Store separately (ex: benzene, carbon tetrachloride).

More detailed chemical compatibility charts available in Part B of the

Chemical SafetyChemical SafetyChemical Storage Chemical Storage ––Minimum Requirements Minimum Requirements

Controlled Products Standard

http://umanitoba.ca/admin/human_resources/ehso/geninfo/cps.html

82

Glacial Acetic Acid Storage

Store glacial acetic acid in a flammable storage cabinet.As the acid is corrosive, secondary containment such as a bottle jacket will be required

Chemical SafetyChemical Storage - Special Notice!

Glacial Acetic Acid is a highly corrosive organic acid with the property of releasing Hydrogen gas when subjected to heat or comes in contact with strong oxidizers such as perchloric and nitric acids. At an LEL between 5.4% and UEL 16%, the acid may explode.

http://www.justritemfg.com/pix/Originals/12009.jpg

83

Chemical SafetyChemical SafetyChemical StorageChemical Storage-- Dangerous Storage CombinationsDangerous Storage Combinations

Oxidizers with Flammables Acids (conc.) with Bases (conc.)

Alkali metals- like Ca, K, Na withwater, CO2, CO, CCl4

Annhydrous Ammonia with halogens, Hg, HF, CaClO4

Acetic Acid with chromic, nitric or perchloric acid, peroxides, permanganates, hydroxides i.e. KOH

Chromic Acid with acetic acid, alcohol, naphthalene, glycerine, and other flammable liquids

Acetone with concentrated sulphuricor nitric acids

H2O2 with flammables, Cu, Cr, Fe or respective salts

Hypochlorites with acids Hydrogen Sulphide with Nitric Acid

Chlorine with ammonia, acetylene, butadiene, benzene, petroleum derivatives, sodium carbides

Chlorates (ClO3) with ammonium salts, acids, metal powders, sulphur, carbon

Cyanides (Alkaline) with acids Potassium chlorate with acids

See CPS Part B Pg. 35-39 for more charts

http://www.umanitoba.ca/admin/human_resources/ehso/geninfo/cps.html

84

Store in a well ventilated room (Note: Cold rooms are not ventilated!)

Transport securely to prevent accidental spillage Store Liquid N2 in a vented Dewer

Risks & Precautions:Risk of frost-biteRisk of samples explodingRisk of asphyxiationAvoid skin contactWear insulated gloves and eye protection

Chemical SafetyChemical Storage -Liquid NLiquid N22 & Dry Ice& Dry Ice

85

Radiation SourcesRadiation Sources

Radiation Emitting Devices (ex: X-rays)LASERsRadioactive Materials

Radioactive chemicalsSmall sealed sources of radioactive materialMachines and equipment containing large sealed sources of radioactive material

Federal Legislation mandates procedures, permits and training for users of radioactive material. Contact EHSO for more information.

Radiation Safety Program

86

Radiation Quick FactsRadiation Quick Facts


Amount of Radioactivity is measured in curies or becquerels1 Ci (curie) = 37 billion disintegrations per second (dps) or 37 GBq (gigabecquerel)Radiation Dose is measured in sieverts (Sv)

3 mSv= Annual environmental radiation exposure for non-smoker in Manitoba1 mSv / yr = CNSC (Federal Gov.) radiation exposure limit for U of M employee30-80mSv= Annual radiation exposure for smoker (1pk per day)50,000-60,000 mSv = radiation used clinically to kill cancer cells

Isotope Half life Type of radiation Shielding required

H-3 12.3 years Low energy beta particle None

C-14 5730 years Low energy beta particle None

S-35 87.5 days Low energy beta particle None

P-33 25.6 days Low energy beta particle None

P-32 14.3 days Mid energy beta particle 1cm lucite or wood

I-125 60.1 days Gamma/X-ray Lead

Cr-51 27.7 days Gamma/X-ray Lead

87

Radiation DetectionRadiation Detection

Scintillation Counterfor weekly contamination monitoring of

H-3, C-14, S-35, P-32, P-33, I-125, Cr-51

γ DetectorI-125, Cr-51

β-Particle DetectorP-32

Detects large amounts of C-14, S-35, P-33

Gamma Counterγ- emittersI-125, Cr-51


88

EHSO Controlled Inventory and Permit System – all users must have appropriate training before beginning work withradioactive materials; contact EHSO Radiation Safety Coordinator for more information or go to their website:

Radiation Safety ManualTrainingInternal InspectionsCNSC InspectionsSecurity for Radioactive Materials Radiation Safety Committee – provides guidance and direction to program development

Program ElementsProgram Elements


EHSO Radiation Safety web-site

http://www.umanitoba.ca/admin/human_resources/ehso/rad_safety/index.htm

89

New User RequirementsNew User Requirements

Every lab that works with radiochemicals MUST have a U of M Internal Radioisotope Permit. This permit lists the radiochemicals that can be used as well as the workers in that lab that are allowed to use these radiochemicals.New lab members can work with radiochemicals once they fulfill the following conditions:1. Complete a Radiation Course (1.5 days) offered by EHSO

Contact Leona Page or Eva Sailerova 789-3613 or 789-33592. Are added to the lab Radioisotope Permit

See your Supervisor3. If required by your Radioisotope Permit,

apply for and obtain a ring orwhole body dosimeter.See your Supervisor or Dept. Office


90

How to keep safeHow to keep safeRadiation Safety Program

All radiochemicals in use at the U of M are usedin trace amounts or are shielded in equipment.Decrease your exposure by:

Not removing shielding Limiting the time you spend in these areasMaximize the distance – it is safe to go within2 meters for a short period of time.Practice good personal hygieneGet training

All radioactive materials MUST be marked with the radioactive symbol (items, use/storage areas).Non-permitted workers in the area of radioactive labs must review the “Radiation Safety Pamphlet” at the link below.

Radiation Safety Pamphlet for Non-Permitted Workers

http://umanitoba.ca/admin/human_resources/ehso/media/RadSafetyPamphletnonpermittedworkers.pdf

http://umanitoba.ca/admin/human_resources/ehso/media/RadSafetyPamphletnonpermittedworkers.pdf

91

Entrance Signs for Permitted AreasEntrance Signs for Permitted Areas

Caution: Entry is permitted but be aware that Basic Level radioactive chemicals or sealed radioactive sources may be present. These will be marked with the trefoil.

The symbols below indicate to everyone the levels of associated potential radioactive hazards. (Explanatory note: Entrance signswere originally developed for caretakers / tradespersons.)

Do Not Enter: Special procedures may be required to reduce radiation hazard when an Intermediate Level radioactive chemical or spill is present. Entry is restricted to:

1) Workers listed on the Intermediate Permit2) Non-permitted workers if with a permitted worker3) Responding to a life or property threatening emergency.

Non-permitted workers are not allowed to touch any material labeled with the trefoil. Report immediately to EHSO.


92

Program ElementsProgram Elements

Biological Safety Program

The U of M Biological Safety Program is administered by EHSO under the direction of the Biological Safety Advisory Committee (BSAC)

The Biosafety Guide is a user resource for determining appropriate facility and operational procedures for working with biological material at the U of M and is based on relative legislation and guidelines

Biosafety Permit Applications register the use of biological materials with the university and are required for Biosafety Project Approvals.

Biosafety Project Approval Certificates for Granting Agency assurances are provided on behalf of the university by the Chair, BSAC, and require that all information registered on Biosafety Permit is current.

EHSO maintains a database of Biosafety Cabinet annual certifications.

New permit requirements have been implemented as of April 30, 2008

U of M Biosafety Website

http://www.umanitoba.ca/admin/human_resources/ehso/chembio_safety/biosafety.html

93

Relevant Legislation and GuidelinesRelevant Legislation and Guidelines


classifies the hazards of biological agents (Risk Groups 1-4) and describes physical and operational standards to work safely withthe different risk groups. (Containment Levels 1-4)

CFIA’s “Containment Standards for Veterinary Laboratories”similar to above but for animal pathogens

U of M Biosafety Guidelines

City of Winnipeg Sharps By-law

Local and Provincial Water and Waste Disposal Laws

Importation and Transportation Legislation

Health Canada’s “Laboratory Biosafety Guidelines”

CFIA’s “Containment Standards for Veterinary Laboratories”

U of M Biosafety Guidelines

http://www.inspection.gc.ca/english/sci/lab/convet/convete.shtml

http://www.inspection.gc.ca/english/sci/lab/convet/convete.shtml




94

Types of Biological Material Included Types of Biological Material Included


Cultured animal cells and the potentially infectious agentswhich these cells may contain. Primate body fluids and other potentially infectious clinical specimens.Tissue or microbial cultures, and materials contaminated by such cultures, stocks or specimens of micro-organisms. Containers or materials saturated with blood products. ParasitesAllergens Tissue from experimental animals including animal dander.Plant viruses, bacteria, fungi. Toxins (bacterial or plant). Vaccines Human anatomical waste (body parts or organs). Animal anatomical waste (carcasses, body parts, organs).

95

Biosafety Permit Requirements Biosafety Permit Requirements


Principal Investigator (PI) Permit ApplicationBiological Agent Inventory and Risk Assessment PI Lab Manual WHIP Lab SignageGeneric and Site-specific Biosafety Training for all persons working

with the agentsBiological Safety Cabinets (if required) certified annuallyEHSO site visitImmunization risk assessment

Biosafety Permit Application instructions can be found on the Biosafety Web site

http://umanitoba.ca/admin/human_resources/ehso/chembio_safety/biosafety.html

96

Top Ten Standard Operational PracticesTop Ten Standard Operational Practices


1. Obtain safety and hazard identification training.2. Wash hands regularly.3. Avoid eating, drinking and application of cosmetics or

contact lenses in lab.4. Never mouth pipette.5. Wear protective clothing including: lab coat, gloves,

eye and face protection.6. Minimize splashes and aerosols (all aerosol-producing

procedures should be done in the BSC).7. Decontaminate waste.8. Restrict or limit access to authorized and trained personnel only.9. Disinfect work surfaces daily and after spill.10. Limit use of sharps.

97

CLIP (Combined Laboratory Inspection Program)

As mandated by relevant regulatory bodies for Chemical, Biological, Radiation, Animal Care and Fire & Life Safety areas, EHSO will conduct periodic and/or annual inspections of all laboratories/workplaces using controlled products.The purpose of inspections is to integrate safety into the workplace by identifying hazards, assessing risks, and finding safer alternatives. Additionally information is gathered to evaluate and improve EHSO programs and training.A Lab Self-Inspection Checklist (SICL) has been developed to assist controlled product users to self-assess their lab in preparation for EHSO site visits and prepare standard operating procedures which are vital to keeping a laboratory/workplace safe and healthy.

For further information, visit the CLIP web-site

http://www.umanitoba.ca/admin/human_resources/ehso/rad_safety/Clip2.html.

98

General Lab SafetyGeneral Lab SafetyPersonal Protective EquipmentPersonal Protective Equipment

Safety EquipmentSafety Equipment

99 General Lab Safety:Potential Laboratory Hazards

Chemicals Includes all six WHMIS classes

Biohazards Cells, animals, biological / patient samples, viruses, bacteria

Allergens Chemical, animal, latex

Radioactive MaterialPhysical / Equipment Hazards

Electrical, sharps, hi/low temperature and pressureMixed hazards

Multiple simultaneous hazards

100

General Lab Safety:Routes of Entry of Hazardous Agents into Body

Inhalation: includes vapours, dust, mists, gases and biological agentsProtection: fume hood, masks, respirator or BSC as appropriate

Absorption: through skin and mucous membranes (eyes, nose, mouth)from splashes, vapours, or indirect contact from hands

Protection: lab coat, correct gloves, hand washing, fumehood use

Ingestion: via contaminated hands, by incidental contamination ofutensils, cups or water bottles or by swallowing phlegm following inhalation of chemicals

Protection: NO eating or drinking or makeup application in the lab

Inoculation: from accidental needle stick injury or cuts from broken glass or other sharps

Protection: limit use of sharps and if possible handle indirectly with forceps

101

Lab coats protect your clothes and your skin in the event of a reagent spill. They also help you avoid bringing contaminated clothing into your home. Must wear when working with controlled products.Should wear at all times when in the lab.Do Not wear lab coats (and gloves) in the following areas:

All offices, bathrooms, elevators, public hallwayCoffee/ lunch rooms, departmental librariesStudent carrel area outside of the lab Other non-lab areas of the building.

Lab CoatsLab Coats

General Lab Safety:Personal Protective Equipment

102


Gloves: Types and CharacteristicsGloves: Types and CharacteristicsLatex: a natural rubber

resists water, acids, alkalis, salts, ketonesNitrile: a synthetic rubber

better chemical protection, superior puncture andabrasion protection, better electrostatic dissipation

Neoprene: a synthetic rubbersuperior chemical protection, good for handling acids, caustics, alcohols, solvents

Vinyl: polyvinyl chlorideshort-term protection against acids, caustics and alcohols

Combination gloves:latex & nitrileneoprene & latexlatex & nitrile & neoprene

Heat & Cold resistant gloves

103 General Lab Safety:Personal Protective Equipment

Gloves: SelectionGloves: Selection

Choose a glove that is appropriate for the task / chemical.

Consult MSDS for any glove specifications

Consult supplier glove charts or check directly with manufacturer if unsure

Check for pin holes in gloves before puttingthem on

Change disposable gloves often


Gloves: RequirementsGloves: RequirementsFor tissue culture 4-6 mil latex or nitrile disposable glove

Handling toxic chemicals Nitrile, neoprene or combo as appropriate for chemical, check MSDS.

Handling corrosive chemicals or solvents

Reusable, chemically resistant, thick gloves, usually greater than 10 mil –nitrile, neoprene or combo-depending on contact time and concentration of chemical, check MSDS. There is one brand of disposable 8 mil nitrile gloves that offers dexterity and some chemical resistance. Contact EHSO for more info.

Working with RNA 4-6mil latex or nitrile disposable glovesRemoving items from the–80°C freezer or liquid

nitrogen

Thermal resistant gloves

Handling hot solutions or items

Thermal resistant gloves

105

Glove Examples

DisposableNitrile

Heat and ColdResistant

Reusable Chemically Resistant

DisposableLatex


Eye Protection / GogglesEye Protection / GogglesEyes are much more sensitive to chemical and physical damage than our skin and may not heal as wellEye protection should be worn at all time in the lab areas but it must be worn when:

- doing radioactive work- handling caustic or dangerously reactive chemicals- retrieving samples from liquid nitrogen- working with UV light (UVA&B filtering face shield)- cleaning chemical spills (non-vented goggles)- working with lasers

Consult with your supervisor to ensure that the available eye protection is appropriate for the task

107

Chemical and Vapour

Resistant Safety

Goggles(no direct

vent openings)Face Shield

Impact-Only Resistant Safety Goggles

(Direct vent holes therefore not chemical splash and vapour resistant)

Basic safety glasses

Eye and Face Protection Options

108


Masks and RespiratorsMasks and RespiratorsMasks protect only from particles / dust or aqueous mists that do NOT emit harmful vapors.Respirators come as full or half face and may be appropriate to work with hazardous vapours when using a fume hood is not feasible.

Respirators come with a variety of cartridges for work with biological and/or chemical material.Use of a respirator (including N95 masks) requires that the user

be fit tested by the EHSO.

Consult with your supervisor and EHSO to determine appropriate use of masks/respirators for your work.

109

Dust Mask

Half and Full Face Respirator(Must be fit-tested)

Powered Air Purifying

Respirator(PAPR)

N95 Masks(Must be fit-tested)

Respiratory Protection Examples

110

Every Lab MUST have the following available:Every Lab MUST have the following available:1. Lab coats2. Gloves (nitrile and latex or latex-like), chemically resistant as

appropriate and thermal resistant3. Eye protection (individual safety glasses, laser eyewear, goggles

and face shield as appropriate)4. Respiratory protection (dust masks, respirator, N95 as required)5. Functional fume hood with adequate space

Fume hoods should NOT be a chemical or equipment storage area, or permanent radioactive workstations. If your hood lacksspace to comfortably decant a 4L bottle of solvent into a beaker then it REQUIRES CLEANING (discuss with Principal Investigator / Supervisor)

General Lab Safety:Mandatory Protective Equipment

111

Hands-free sinkEye washSafety shower

Fire ExtinguishersSmoke AlarmsSprinklers

General Lab Safety:Safety Equipment

112

Fume HoodsFume HoodsDraws air from the lab through an opening in the sash. Contaminated air is expelled out an exhaust duct on the roof of the building. There is generally NO filtration of contaminated air. U of M air flow standards require a face velocity of 80-120 lfpm at a sash height of 11in (30cm). Larger sash heights decrease air flow while smaller sash heights increase air flow creating turbulence.

Protection:Offers personal protection only, NO sample or environmental protection.

Uses:Laboratory work involving poisonous, corrosive, odorous or flammable chemicals (if appropriate).

General Rules:Work with sash at height indicated on stickerWork with material at least 15cm behind face of sashDo not block rear baffles Do not use to dispose of solvents by evaporationDo not use for general chemical storage


113

Biological Safety Cabinets (BSC)Biological Safety Cabinets (BSC)Specially designed cabinet where laminar flowing air within the hood is HEPA filtered. When used properly the environment within the hood is sterile and operators are protected from exposure to biohazardous aerosols. Exhausted air from the hood is either HEPA filtered and blown into the lab or HEPA filtered and exhausted directly outdoors through ducting.

Protection:Offers personal, sample and environmental protection from biohazardous aerosols

Uses:Work with aerosol producing Risk Group2 organisms.

General Rules:Don’t disrupt laminar flow by blocking vents, overcrowding, by rapid lateral movements within or outside the hood, or by using a flameDon’t use with chemicalsDon’t disinfect with bleach alone unless followed with appropriate rinse. Bleach corrodes stainless steel.Don’t operate with UV light engaged


114 General Lab Safety:Safety Equipment

Taken from: http://www.cdc.gov/od/ohs/biosfty/bsc/bsc.htm#contents

Biological Safety Cabinets Work LayoutBiological Safety Cabinets Work LayoutA typical layout for working "clean to dirty" within a Class II BSC. Clean cultures (left) can be inoculated (center); contaminated pipettes can be discarded in the shallow pan and other contaminated materials can be placed in the biohazard bag (right). This arrangement is reversed for left-handed persons.

http://www.cdc.gov/od/ohs/biosfty/bsc/bsc.htm#contents

115


Vacuum Flask Set-up

The collection and trap flask should be placed in secondary containment.

116

Clean BenchClean BenchSpecially designed cabinet that provide vertical or horizontal HEPA fill laminar flowing air over the work space.

Protection:Offers sample protection only NO personal or environmental protection.

Uses:For lab work requiring a sterile environment that does NOT involve Risk Group 2 organisms. For example media preparation or plant culturing is fine but NOT tissue or cell culturing.

General Rules:Don’t disrupt laminar flow by blocking vents, overcrowding, by rapid lateral movements within or outside the hood.Don’t use with chemicalsDon’t disinfect with bleach alone unless followed with appropriate rinse. Bleach corrodes stainless steel.


117


Safe Transport of Controlled Products

Protective Container

Chemical Transport Cart

Note siderails, secondary containment and large wheels

118

General Lab SafetyGeneral Lab Safety

U of M Waste Disposal Procedures

119

Segregate Biological from Chemical from Radioactivewaste at source.

NO sink disposal of chemicals(with limited exceptions as per U of M waste disposal chart)

Develop waste disposal procedures before starting work.

Generation of mixed waste (e.g. radioactive and biological) may need special procedures and approval.

Questions should be directed to Environmental Management Coordinator, Paul Houle, at UM EHSO 474-6316

General GuidelinesGeneral Guidelines

General Lab Safety:Waste Disposal Procedures

120

Glass/Sharpsincludes broken glass, rigid plastic tips, pasteur pipettes, microscope slides (anything that can puncture the skin)

Chemicalsolid and liquid

Biohazardous / Biomedical materialsolid, liquid, sharps including all needles, syringes, razorand scalpel blades

Radioactive materialsolid and liquid

Old lab equipmente.g. centrifuges, incubators, refrigerators, freezers

Types of WasteTypes of Waste


121

NONO chemical, biological or radioactive contaminateschemical, biological or radioactive contaminatesPrepare a box lined with

a heavy plastic bag oruse a purchased glass/sharps disposal

container (Available from UM caretaking services)

Once fulla) seal plastic bagb) seal box with tapec) mark as ‘GLASS WASTE’ and dispose

with caretaker (UM) or directly in garbage bins (HSC)

Deface any inappropriate label on box before discarding

Glass WasteGlass Waste


122

ChemicalsChemicals

All chemicals must be disposed via UM EHSOAny generated waste must be labeled with the U of M Waste Tagwhile being collected or stored in lab. No evaporation of organics in the fume hood – cap bottles tightly.

Sink disposal (followed by copious amounts of water)is limited to:1) buffer or detergent or mild acid / base solutions (<1%) 2) <20% v/v methanol or ethanol solutions3) bleach containing solutions

(usually 1:5 dilution of stock or ~1% sodium hypochlorite)


U of M Hazardous Waste Tag

http://www.umanitoba.ca/admin/human_resources/ehso/emanagement/haz_waste_forms.html

123

Chemicals (continued)Chemicals (continued)


Chemicals or organics cannot be diluted with water for thepurpose of disposalEmpty bottles from toxic chemicals (acute & chronic) mustbe disposed via UM EHSOSome chemicals become explosive with extended storage and must be disposed before the expiry date (eg. ether, picric acid, tetrahydrofuran)Any questions regarding chemical waste disposal should be directed to Paul Houle at UM EHSO 474-6316

A complete list of disposal procedures is available at theU of M Hazardous Waste web-site

http://www.umanitoba.ca/admin/human_resources/ehso/emanagement/hazwaste.html

124

Decant the solvent drum completely.Do not pour contents down the sink.Do not rinse.Leave drum open in fume hood to allow residual solvent to evaporate.Once dry, deface all signs and symbolson the surface of the container.Discard container as regular waste with caretakers.

20L Ethanol or Methanol Drums 20L Ethanol or Methanol Drums


20L Drums from other solvents20L Drums from other solventsDo not evaporate contents.Dispose as chemical waste through the EHSO

XXXXXX

XX

125

Complete the chemical waste disposal form.

Bannatyne Campus Labs:Transport chemicals in secondary containment to T152 Basic Sciences Building between 11AM – 12PM on the days specified on the Chemical Waste Disposal Calendar

Fort Garry Campus Labs:Fax form to Hazardous Waste Coordinator at 474-7629 and wait for pick-up

Chemicals Waste: Solid and LiquidChemicals Waste: Solid and Liquid


Chemical Waste Disposal Forms

Waste Disposal Calendar

Chemical Transport Cart





126

Aspirate or pour off all liquids and dispose into designated biohazardous waste container lined with clear autoclave bags . This waste should contain NO hazardous chemicals.

BiohazardousBiohazardous Waste: Solid and DryWaste: Solid and Dry


Plain Clear Autoclave Bag

Note Large LabelUsed petri dishes, plasticware,

culture flasks, bench paper, gloves

127

Fill the bag only to the level where it can be taped shut, label and submit for autoclaving. Transport in secondary containment ifmoving between floors . During autoclaving the bag must be open to allow steam to access contents.After autoclaving place in green garbage bag and dispose as regular garbage.

BiohazardousBiohazardous Waste: Solid and Dry (continued)Waste: Solid and Dry (continued)

Note Large Label


EKF Lab

Minimum 1 hour @ 121°C

Regular garbage

Tape shut and label

http://images.google.com/imgres?imgurl=http://www.public.iastate.edu/~health/tour/images/autoclave.jpg&imgrefurl=http://www.public.iastate.edu/~health/tour/images/&h=500&w=375&sz=23&tbnid=Low5C1RVuHwJ:&tbnh=127&tbnw=95&start=4&prev=/images%3Fq%3Dautoclave%26hl%3Den%26lr%3D%26sa%3DG

http://images.google.com/imgres?imgurl=http://www.smm.org/sln/tf/k/kites/garbage.gif&imgrefurl=http://www.smm.org/sln/tf/k/kites/kites.html&h=199&w=170&sz=21&tbnid=xFQelUQviIUJ:&tbnh=98&tbnw=84&start=1&prev=/images%3Fq%3Dgarbage%2Bbag%26hl%3Den%26lr%3D%26sa%3DN

128

For the following:1) ALL needles, syringes, scalpel or razor blades2) ALL potential sharps contaminated

with human / animal tissue, fluids or blood.Dispose into a rigid, puncture-resistant, appropriately labeled containers , and once the container is ¾ filled, autoclave and discarded through the EHSO.

BiohazardousBiohazardous Waste: SharpsWaste: Sharps


Precautions:Do NOT remove needles from syringe or recapDo NOT bend needlesDo NOT overfill containers (greater than ¾ full)Do NOT autoclave chemically or radioactively contaminated sharps. These are placed in separate containers, labeled appropriately and given to the EHSO�.

http://images.google.com/imgres?imgurl=http://www.ehss.vt.edu/Programs/LSD/Biosafety/BiosafetyForLaboratoryWorkers/Images/BiohazardSymbol.jpg&imgrefurl=http://www.ehss.vt.edu/Programs/LSD/Biosafety/BiosafetyForLaboratoryWorkers/04_AdministrativeControls.htm&h=675&w=750&sz=24&tbnid=oeAUPgpXnXsJ:&tbnh=126&tbnw=140&start=62&prev=/images%3Fq%3Dsharps%2Bcontainers%26start%3D60%26hl%3Den%26lr%3D%26sa%3DN

http://images.google.com/imgres?imgurl=http://www.minresco.com/gif/trefoil.gif&imgrefurl=http://www.minresco.com/radioactive/radioactive03.htm&h=50&w=53&sz=2&tbnid=H4EK8Sf-5NAJ:&tbnh=50&tbnw=53&start=3&prev=/images%3Fq%3Dradioactive%2Btrefoil%26hl%3Den%26lr%3D%26sa%3DG

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129

For potential sharps contaminated with microbiological substances: e.g. bacteria, viruses and tissue cultures

Collected in rigid reusable containers , Autoclave and dispose as regular glassware waste.

BiohazardousBiohazardous Waste: Sharps (continued)Waste: Sharps (continued)


Sharps contaminated with human or animal fluid (excluding blades, needles and syringes)

Rigid, puncture-resistant, reusable containers that can be autoclaved

Discard as regular glassware waste

Autoclaveminimum1hr @ 121oC

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130

BiohazardousBiohazardous Waste: Liquids and Pathological Waste: Liquids and Pathological


Treat with a proven chemical method

(eg bleach)– OR –

Autoclave @ 121°C as appropriate for volume

Pathological Waste(e.g. animal body parts and carcasses)

RadioactivePathological Waste

Double bag and store carcasses and all related material in designated freezer for pick-up by Central Animal Care Services (CACS)

Requires pre-approval from Radiation Safety Program and CACS

non-chemicalnon-radioactive

Liquid Biohazard Waste

Dispose in sink with LOTS of water

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131

Radioactive Waste: Dry and LiquidRadioactive Waste: Dry and Liquid


All radioactive waste is disposed via U of M EHSO.

Collect each isotope separately.

Collect radioactive solid and liquid waste separately in approved containers.

Collect radioactive scintillation fluid and aqueous liquid waste separately.

Complete a yellow Radioactive Waste Tag, record activity in MBq and attach to container

For all liquid waste:write the full trade name or full chemical name of all the components on the tag.Attach the yellow waste tag to bottles of liquid with a string or twine.

“Yellow Tag” attached with string on radioactive liquid

waste container

Radioactive Waste Disposal Guidelinesand Calendar

http://www.umanitoba.ca/admin/human_resources/ehso/rad_safety/index.html

http://www.umanitoba.ca/admin/human_resources/ehso/rad_safety/index.html

132

Laboratory Equipment Disposal & Decommissioning:

U of M Decommissioning/Decontamination Form required for Laboratory Equipment leaving the laboratory for disposal or servicing

The principal investigator or departmental designate is responsible for ensuring that cleaning is done by trained lab personnel. Caretakers are NOT appropriate, and do not provide this service.

Equipment for disposal must be labeled with the Equipment Disposal sticker.

Forms, labels and procedures for cleaning and disposing of equipment are available from EHSO.

General Lab Safety: Waste Disposal Procedures

U of M Equipment Decommissioning Procedure

http://www.umanitoba.ca/admin/human_resources/ehso/chembio_safety/Decomm.html

133

*Charts should be posted in all labs andare available from the EHSO- Call 474-6633

The University of Manitoba WASTE DISPOSAL CHART FOR LABORATORIES

Laboratory Waste

Disposal through EHSO (474-6633)Method of Disposal determined by EHSO.

Incinerate Licensed Commercial Hazardous Waste Handler

Brady Landfill

Collect in a suitable container. Store Securely. Follow the EHSO Environmental Management Protocol. Fax a completed Hazardous Waste Form to EHSO (474-7629). At Bannatyne Campus, bring the completed Hazardous Waste Form along with the Hazardous Waste in Secondary Containment to Room T152, Basic Sciences Building on the scheduled days.

needles, blades, scalpels, tips. Pack chemically involved in puncture-proof containers. Pack biomedically involved in autoclavable sharps containers that must be autoclaved (see note 6).

Pour down a laboratory drain. Flush with copious amounts of cold water

1. NO CHEMICALS ARE TO BE LEFT FOR THE UNIVERSITY OF MANITOBA CUSTODIAL STAFF. 2. All hazardous waste must be labelled with EHSO Hazardous Waste Labels or original labels if original contents. 3. Empty reagent bottles are to be rinsed and have the labels defaced. 4. Contact manufacturer/supplier (e.g. Matheson) for disposal of empty/unused compressed gas cylinders (e.g. Lecture bottles etc.); contact EHSO for consultation. 5. Animal carcasses are to be incinerated. 6. All potentially infectious materials (biomedically involved) must be autoclaved or deactivated using a chemical sterilizing agent prior to disposal. 7. Consult also the University of Manitoba Biohazardous Waste Disposal Guidelines. 8. Radioisotope users should consult the University of Manitoba “Waste Disposal Chart for Radioisotope Users”.

134

*Charts should be posted in all labs and are available from the

EHSO-Call 474-6633

-or-

See last page of Biosafety Guide

Biohazadous Disposal Chart

Biohazard Waste Chart

http://www.umanitoba.ca/admin/human_resources/ehso/media/Bio_waste_Chart_pictures.pdf

http://www.umanitoba.ca/admin/human_resources/ehso/media/Bio_waste_Chart_pictures.pdf

136

Dealing withDealing withNonNon--Hazardous & Hazardous Hazardous & Hazardous

Chemical SpillsChemical Spills

General Lab SafetyGeneral Lab Safety

137

General Lab Safety:Spills - General Guidelines

Spills should never be cleaned up by untrained staff.Caretakers are not allowed to clean up any lab spills.The best time to learn about and practice cleaning up a spill is before it happens.Every lab that contains controlled products should have a basic spill kit.Spill kit location and use must be known by all lab members.

138 General Lab Safety:Spill - General Guidelines

Basic Lab Spill KitBasic Lab Spill KitThe “Basic Lab Spill Kit” should include the following:

1) A five gallon pail with lid for kit storage or to act as a wastepail in the event of a spill.

2) Universal absorbent pad and granular absorbent.3) Disposable and reusable

chemically resistant gloves.4) Non-vented splash goggles.5) Caution tape and duct tape.6) Sealed plastic transfer pipettes.7) Dustpan and brush.8) Disposal and autoclave bags.

139 General Lab Safety:Spills - General Guidelines

Additional Departmental Spill Kit SuppliesAdditional Departmental Spill Kit SuppliesIn addition to basic lab spill kits, Departments should also provide the following:

1) Emergency contact lists.2) Commercial mop and pail.3) Full size “washable” broom and dustpan. 4) Respirator with multi-purpose P100 cartridges

suitable for acids, ammonia, amines, chlorineorganic vapours as well as fine particulates,and dusts.

5) Additional “emergency” stash of disposable supplies like universal pads, caution tape, gloves, disposable bags and autoclave bags.


Basic spill typesBasic spill typesGlasswareBreakage

Non-hazardousLiquids & Solids

HazardousLiquids &Solids

Biohazardousmaterial

Radioactivematerial

Incident Report

Incident Report

Incident Report

Please file a departmental incident report for hazardous, biohazardousor radioactive spills.

An incident report should also be filed for any spills where a personal injury occurs.

141 General Lab SafetySpills - General Guidelines

Primary ConsiderationsPrimary ConsiderationsGlasswareBreakage




Radioactivematerial

1) Remain calm, tell other lab membersand ask for help.

2) Deal with personal or co-worker injuries or potential injuries (for example remove all contaminated clothing and wash contaminated skin).

3) If liquid is involved, obtain your lab spill kit and contain the spill with absorbent materials.

4) Tape off the area to avoid people from entering the spill zone.

5) Assess the cleanup procedure and obtain necessary equipment.

6) Off hours Inform Security Services

Incident Report

Incident Report

Incident Report


Glassware BreakageGlassware BreakageGlasswareBreakage




Radioactivematerial

Sweep up broken glass and discard as glassware waste.

Glassware Waste


NonNon--Hazardous Liquids and SolidsHazardous Liquids and SolidsGlasswareBreakage




Radioactivematerial

Wear a lab coat and spill kit gloves and dust mask (if necessary).

Sweep up solid chemical and broken glass and discard in a waste container labeled with the UM waste tag. Once done, cap and arrange for disposal via U of M EHSO.

For liquids, absorb with spill kit absorbent or paper towels or with a mop and discard in a waste container labeled with the UM waste tag. Arrange with UM EHSO for disposal. Wash the area with warm soapy water.

Clean and replace all cleanup equipment.


Hazardous Chemical SpillsHazardous Chemical SpillsGlasswareBreakage




Radioactivematerial

You will NOT attempt to clean up the spill:

1) If you are unaware of the compositionof the spill.

2) If the spill involves more than onechemical.

3) If the cleanup is beyond your capabilities.

If possible to do safely, contain the spill using the special absorbent pad from the spill kit and then call UM EHSO or your emergency contacts for assistance.

Life threatening situation can ONLY be handled by WFPS. Call 555 or 55(MICB).

Incident Report

WFPS= Winnipeg Fire & Paramedic Service


Hazardous Chemical SpillsHazardous Chemical SpillsGlasswareBreakage




Radioactivematerial

Wear a lab coat, spill kit gloves, safety goggles & appropriate respiratory protection.

For solids, sweep up chemical and broken glass and discard in waste pail.

For liquids, absorb with spill kit absorbents and discard in waste pail.

Once all waste has been collected cap the waste pail, label with the U of M waste tag and arrange for disposal via UofM EHSO.

Wash spill area with warm soapy water. If spilt chemical reacts with water seek assistance from EHSO.

Clean and / or replace all cleanup equipment.

Incident Report


BiohazardousBiohazardous SpillsSpillsGlasswareBreakage




Radioactivematerial

Restrict assess to area for 30 minutes to allow aerosols to settle.Wear a lab coat, heavy spill kit gloves, safety goggles & other face protection if required. Absorb all liquid with absorbent material in spill kit. Discard into double clear autoclave bags for autoclaving. Sweep up or use forceps to pick up any broken glass. Discard in disposable biological sharps autoclave waste container.Disinfect area with bleach or disinfectant for the appropriate time and then wash the spill area with warm soapy water.Autoclave or disinfect and replace all cleanup equipment.

Incident Report


Radioactive SpillsRadioactive SpillsGlasswareBreakage




Radioactivematerial

Minimize contaminated area,Minimize amount of wasteDo not spread accidentally

Wear a lab coat and disposable latexor nitrile gloves.

Absorb spill with minimal absorbent material.

Clean area with damp towel from outside in, monitoring success of cleanup.

Discard all soiled materials as radioactive waste (yellow tags)

All large spills MUST be reported to EHSO’sEva Sailerova or Leona Page

Incident Report


Getting Help from EHSOGetting Help from EHSOGlasswareBreakage




Radioactivematerial

You should get help from EHSO:1) If you are unaware of the composition of

the spill.2) If the spill involves more than one

chemical.3) If the cleanup is beyond your capabilities.Where possible, apply spill kit absorbent material, clear and secure the area and call:

UM Environmental Health and Safety474-6633 (9AM-5PM) or 474-9341 or 555 [UM] or 55 [HSC] (non-working hrs)Life threatening situation can ONLY be handled by WFPS. Call 555 or 55(MICB).

Incident Report

Incident Report

Incident Report

149

General Lab SafetyGeneral Lab SafetyBlood, Body Fluids or Other Blood, Body Fluids or Other

Biohazard ExposureBiohazard Exposure& Immunization& Immunization

150 General Lab SafetyBiohazard Exposure: Post-Exposure ProceduresHuman blood, primary cell lines or body fluids1) Get immediate first aid2) If puncture injury: a) encourage bleeding of injury site

b) wash injury site thoroughly with soap and waterc) cover area with sterile dressing if necessary

3) If eye/mucosa splash – flush with water for 15 minutes4) Report incident to supervisor 5) Seek medical advise within 1-2 hours as listed below. Bring your

immunization record if possible.6) Following treatment, file incident report.

Incident Report

Bannatyne Campus Staff

Call Occupational and Environmental Safety & Health (OESH) 787-3312 If no response proceed to HSC Emergency within 2 hours of exposure.

OESH Occupational Health Nurse NA618 - 700 McDermot Ave.

Monday to Friday: 8:30 am - 4:30 pm

If Exposure Occurs outside the above stated hours:Call OESH. Leave message stating name, faculty or department phone number and exposure circumstances. Proceed to HSC Emergency Clinic for assessment.

151 General Lab SafetyBiohazard Exposure: Post-Exposure ProceduresHuman blood or body fluids (continued) Incident Report

Fort Garry Campus Staff

Call the U of M Occupational Health Coordinator 474-6633If no response proceed to Victoria Hospital Emergency within 2 hours of exposure.

Occupational Health CoordinatorEnvironmental Health and Safety Office

191 Frank Kennedy BuildingMonday to Friday: 8:00 am - 4:00 pm

If Exposure Occurs outside the above stated hours:Report directly to Victoria Hospital Emergency Department, 2340 Pembina Hwy. Contact the U of M Occupational Health Coordinator the next day.

University Staff at St. Boniface Hospital or Research Centre

Call the St. Boniface Occupational Health Coordinator 237-2439

Occupational Health NurseSt. Boniface General Hospital Occupational

Health and SafetyMonday to Friday: 7:30 am - 4:00 pm

If Exposure Occurs outside the above stated hours:Report directly to St. Boniface General Hospital Emergency. Contact the St. Boniface Occupational Health Coordinator the next day.

152

General Lab SafetyBiohazard Exposure: Post-Exposure Procedures

Other exposures including animal blood or body fluids andtissue culture cell lines

Encourage bleeding.Wash well with soap and warm water.If necessary, report to appropriate Emergency Departmentfor tetanus or diptheria shot.

Incident Report

153

General Lab SafetyObtaining proper immunization

The principle of Universal Precautions assumes that all human blood and some body fluids are assumed to be positive for any blood-borne pathogens (e.g. HIV, Hepatitis B or C). The expectationis that all work is done with these materials according to Containment Level 2 facility design and operational procedures of Health Canada’s “Laboratory Biosafety Guidelines”.If you are expected to work with human samples or with animals,you should be immunized against Hepatitis B and Tetanus / Diphtheria respectively.See your supervisor or contact the Occupational Health Coordinator (EHSO) to obtain vaccination information about these or othervaccine-preventable pathogens

U of M Immunization Standard

http://www.umanitoba.ca/admin/human_resources/ehso/occ_health_comp/Immunization.html

154

Bleach (sodium hypochlorite) will corrode stainless steel if left in contact for an extended time.

To clean stainless steel equipment (BSC, incubators, water baths, sinks) use 70% ethanol or other non-corrosive disinfectant.

If you must use bleach to clean stainless steel equipment be sure to rinse off all the bleach with water.

Quick Note #1 Bleach and Stainless Steel

155

Shared research areas (e.g. cold rooms, specialized equipment rooms) or shared equipment (e.g. centrifuges, shaker incubators,microscopes) must be maintained by the individuals using this space or equipment.

Individual users are responsible to:1) Wipe down all equipment and report any problems

to department designate.2) Appropriately clean up all spills and wipe down

or wash counter tops.3) Discard old reagents, culture plates and solutions.4) Regularly sweep up the floors in areas that are NOT

entered by caretakers (e.g. cold rooms).

Quick Note #2 Joint Use Area & Equipment Cleanup

156 WHMIS and Safety Training:Self Assessment: You have now completed the Basic Laboratory Safety and WHMIS course.

To access your understanding of the course, review the sample questions on the following slides.

Once you are confident you can answer these types of questions you will arrange to meet with your departmental WHMIS Coordinator to complete your department’s official WHMIS Safety Test.

With the satisfactory completion of the WHMIS Safety Test your WHMIS coordinator will complete and sign his sections of the ‘Laboratory Safety Checklist for New Lab Personnel’. You will then need to meet with your direct employer* or official supervisor** to complete the other site-specific sections of the ‘Laboratory Safety Checklist’ and your lab safety training.

The completed and signed ‘New Laboratory Personnel Safety Checklist’ will then be returned to your departmental office. * Usually the principal investigator

** An individual whose supervisory duties are defined in their job description.

Laboratory Safety Check List for New Lab Personnel

http://umanitoba.ca/admin/human_resources/ehso/images/Laboratory_Safety_Checklist_for_New_Lab_Personnel_revised_Dec_13_07.doc

157 WHMIS and Safety Training:Self Assessment: Review Questions1) Who is your departmental WHMIS coordinator?

2) What does WHMIS stand for?What does MSDS stand for?

3) True or False- The principle goal of WHMIS training is to reduce employee injury and illness from exposure to hazardous materials in their workplace?

4) In your lab where is the nearest:Fire extinguisher?Safety shower?Eye wash station?Spill kit?

5) True or False- You will be discarding chemicals as described in the MSDS?

158 WHMIS and Safety Training:Self Assessment: Review Questions6) An MSDS should be consulted :

a) Only by safety professionalsb) Only in an emergencyc) Before you use a controlled productd) Occasionally to review hazard and precautionary informatione) a and df)c and d

7) Define the following symbols:

8) True or False – A controlled product could fall under more than one hazardous class and as such should have multiple symbols on its corresponding supplier label?

159 WHMIS and Safety Training:Self Assessment: Review Questions9) Three pieces of information required on a workplace label are:

a) Supplier identifier, product identifier and MSDS referenceb) Product identifier, emergency phone and WHMIS symbolc) Product identifier, risk & safe handling info, MSDS referenced) None of the above

10) Should controlled product clean-ups be done by house keeping staff? Why or why not?

11) True or False – Where appropriate, fumehoods should be used for any operation that might release chemical vapours or dusts?

12) True or False – I should ONLY autoclave aqueous solutions (or solids contaminated with aqueous solutions) that can support biologicalgrowth?

13) How do you control exposure to biohazardous substances?

14) Is bleach appropriate for cleaning a biosafetycabinet? Please explain?

160 WHMIS and Safety Training:Self Assessment: Review Questions15) What is the correct procedure for cleaning up a:

a) Toxic and volatile liquid chemical spill?b) Biohazardous spill outside the biosafety cabinet?c) Non-toxic solid chemical spill?

16) What is the location of your lab MSDSs?

17) Which of the following can NOT be disposed down the sink?a) 10% solution of SDS detergentb) 10X PBS (phosphate buffered saline)c) bleach neutralized baterial media from a liquid cultured) a solution containing 30% ethanol

18) List 3 places in your department where it is NOT appropriate to wear gloves or a lab coat even if they are clean.

19) When is it appropriate to eat or drink in the lab?

20) How would you decide what would be the bestglove to wear when working with a controlled product?

161 WHMIS and Safety Training:Self Assessment: Answers1) Who is your departmental WHMIS coordinator?

Department specific

2) What does WHMIS stand for?Workplace Hazardous Materials Information SystemWhat does MSDS stand for?Material Safety Data Sheet

3) True or False- The principle goal of WHMIS training is to reduce employee injury and illness from exposure to hazardous materials in their workplace?

4) In your lab where is the nearest:Fire extinguisher? Department / Lab specificSafety shower? Department / Lab specificEye wash station? Department / Lab specificSpill kit? Lab specific

5) True or False- You will be discarding chemicals as described in the MSDS? All chemicals are discarded through EHSO

162 WHMIS and Safety Training:Self Assessment: Answers6) An MSDS should be consulted :

a) Only by safety professionalsb) Only in an emergencyc) Before you use a controlled productd) Occasionally to review hazard and precautionary informatione) a and df) c and d

7) Define the following symbols: See slides 33 - 63

8) True or False – A controlled product could fall under more than one hazardous class and as such should have multiple symbols on its corresponding supplier label?

163 WHMIS and Safety Training:Self Assessment: Answers9) Three pieces of information required on a workplace label are:

a) Supplier identifier, product identifier and MSDS referenceb) Product identifier, emergency phone and WHMIS symbolc) Product identifier, risk & safe handling info, MSDS referenced) None of the above

10) Should controlled product clean-ups be done by house keeping staff? Why or why not? No, they do NOT have appropriate training.

11) True or False – Where appropriate, fumehoods should be used for any operation that might release chemical vapours or dusts?

12) True or False – I should ONLY autoclave aqueous solutions (or solids contaminated with aqueous solutions) that can supportbiological growth? Chemical or chemical solutions in which biological life can not grow, should never be autoclaved.

13) How do you control exposure to biohazardous substances? See slide 96

14) Is bleach appropriate for cleaning a biosafetycabinet? Please explain? See slide 154

164 WHMIS and Safety Training:Self Assessment: Answers15) What is the correct procedure for cleaning up a:

a) Toxic and volatile liquid chemical spill? See slide 144-145b) Biohazardous spill outside the biosafety cabinet? See slide146c) Non-toxic solid chemical spill? See slide 143

16) What is the location of your lab MSDSs? Lab specific

17) Which of the following can NOT be disposed down the sink?a) 10% solution of SDS detergentb) 10X PBS (phosphate buffered saline)c) bleach neutralized baterial media from a liquid cultured) a solution containing 30% ethanol

18) List 3 places in your department where it is NOT appropriate to wear gloves or a lab coat even if they are clean. See slide 101

19) When is it appropriate to eat or drink in the lab? NEVER!!!

20) How would you decide what would be the bestglove to wear when working with a controlled product? Refer to the MSDS

165 WHMIS and Safety Training:Self Assessment:Contact Your WHMIS Coordinator

Thank you for completing the University of Manitoba Basic Lab Safety & WHMIS Course.

You must now contact your departmental WHMIS coordinator to complete the WHMIS and Lab Safety Testand the Laboratory Safety Check List For New Lab Personnel.

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