Anatomy of the roots of nine species of Bromeliaceae (Poales) from ...
Barcoding of Bromeliaceae (Poales) - IBOL 2017 · Bromeliaceae will be provided based on the...
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Barcoding of Bromeliaceae (Poales) 1,2 Cyrannek, N., 1,2 Heller, S., 3 Leme, E.M.C., 1,2 Favre, A., 1,2 Paule, J., 4 Koch, M. & 1,2 Zizka, G. 1 Department Botany and Molecular Evolution, Senckenberg Research Institute Frankfurt, Germany 2 Institute Ecology, Evolution and Diversity, Faculty Biosciences, Goethe University Frankfurt, Germany 3 Marie Selby Botanical Gardens, Sarasota, Florida, USA 4 Center of Organismal Studies, University Heidelberg, Germany Background The angiosperm family Bromeliaceae comprises 3912 species (Butcher & Gouda 2017: http://botu07.bio.uu.nl/bcg/taxonList.php), almost all of them restricted to the Neotropics (a single species is native to West Africa). The family is characterized by exceptionally high morphological and ecological diversity as well as a very low DNA sequence variability. Most of the species are monocarpic, propagating vegetatively with lateral shoots. In several genera, plants are vegetatively very similar, which makes determination of bromeliads difficult (fig. 2). Especially in Botanic Gardens this is a problem, when plants are cultivated several years before flowering. Barcoding is a very promising approach to provide fast and cheap determination of bromeliads, however, the observed low genetic variability causes specific problems. Results In the course of a project (Evo-BoGa) funded by the German Federal Ministry of Education and Research (BMBF) to improve access and scientific use of living collections, a number of DNA markers was tested for their suitability for barcoding (nuclear: agt1, ETS, phyC; plastid: matK, ycf1, trnL-F, psbA- trnH). The highly variable nuclear marker agt1 was identified as suitable barcode marker for identification. Significance The barcoding approach will improve the availability of bromeliad collections in Botanic Gardens. Outlook At present, app. 400 species of subfamily Bromelioideae have been barcoded, app. further 500 species will be sequenced in the course of the project. A special web-portal for Bromeliaceae will be provided based on the BrassiBase database (URL: https://brassibase.cos.uni-heidelberg.de). Acknowledgements Federal Minstery of Education and Research Epiphytic bromeliad diversity in Panama (Foto: Daniel Caceres). Guzmania musaica, a tank forming bromeliad in flower (Foto: Daniel Caceres). Bromeliad collection in the private Botanic Garden “Refúgio do Gravatás” in Teresopolis. Note morphological similarity and very few specimens in flower (Foto: Sascha Heller). Alignment of agt1 with a highly variable part (left), a large indel (center) and the highly conserved part (right). Pairwise sequence similarity for agt1 for selected species of Bromelioideae throughout the subfamily. The marker agt1 showed exceptional variability. Other recommended barcoding markers for plants (e.g. matK, rbcL, psbA-trnH) did not provide sufficient resolution. ID 435
Transcript of Barcoding of Bromeliaceae (Poales) - IBOL 2017 · Bromeliaceae will be provided based on the...
Barcoding of Bromeliaceae (Poales) 1,2 Cyrannek, N., 1,2 Heller, S., 3Leme, E.M.C., 1,2 Favre, A., 1,2 Paule, J., 4 Koch, M. & 1,2 Zizka, G.
1 Department Botany and Molecular Evolution, Senckenberg Research Institute Frankfurt, Germany 2 Institute Ecology, Evolution and Diversity, Faculty Biosciences, Goethe University Frankfurt, Germany
3 Marie Selby Botanical Gardens, Sarasota, Florida, USA 4 Center of Organismal Studies, University Heidelberg, Germany
Background
The angiosperm family Bromeliaceae comprises 3912 species (Butcher
& Gouda 2017: http://botu07.bio.uu.nl/bcg/taxonList.php), almost all of
them restricted to the Neotropics (a single species is native to West
Africa). The family is characterized by exceptionally high morphological
and ecological diversity as well as a very low DNA sequence variability.
Most of the species are monocarpic, propagating vegetatively with
lateral shoots. In several genera, plants are vegetatively very similar,
which makes determination of bromeliads difficult (fig. 2). Especially in
Botanic Gardens this is a problem, when plants are cultivated several
years before flowering. Barcoding is a very promising approach to
provide fast and cheap determination of bromeliads, however, the
observed low genetic variability causes specific problems.
Results
In the course of a project (Evo-BoGa)
funded by the German Federal Ministry of
Education and Research (BMBF) to improve
access and scientific use of living collections,
a number of DNA markers was tested for
their suitability for barcoding (nuclear: agt1,
ETS, phyC; plastid: matK, ycf1, trnL-F, psbA-
trnH).
The highly variable nuclear marker agt1 was
identified as suitable barcode marker for
identification.
Significance
The barcoding approach will improve the
availability of bromeliad collections in Botanic
Gardens.
Outlook
At present, app. 400 species of subfamily
Bromelioideae have been barcoded, app.
further 500 species will be sequenced in the
course of the project. A special web-portal for
Bromeliaceae will be provided based on the
BrassiBase database
(URL: https://brassibase.cos.uni-heidelberg.de).
Acknowledgements
Federal Minstery of
Education and Research
Epiphytic bromeliad diversity in Panama (Foto: Daniel Caceres).
Guzmania musaica, a tank forming bromeliad in flower (Foto: Daniel
Caceres).
Bromeliad collection in the private Botanic Garden “Refúgio do Gravatás” in Teresopolis. Note morphological similarity and very few
specimens in flower (Foto: Sascha Heller).
Alignment of agt1 with a highly variable part (left), a large indel (center) and the highly conserved part (right).
Pairwise sequence similarity for agt1 for selected species of Bromelioideae throughout the subfamily. The marker agt1 showed
exceptional variability. Other recommended barcoding markers for plants (e.g. matK, rbcL, psbA-trnH) did not provide sufficient
resolution.
ID 435
