Automating SPE Extractions with the HT400E System

by

William AmoyalDisruptive Technologies

Disruptive Technologies history

• Distribution company founded in 2005• Based in Villecresnes, France• Cover France, Belgium, Switzerland and other

countries depending on product range• Agilent Service Partner for UV/Vis

spectrophotometry, HPLC and CE• 2 people

– William Amoyal, Sales Manager– Franck Do Vale, Service Manager

Disruptive Technologies product range

• Sample Preparation– Nucleic acides, protein and

small molecules extraction from hard-to-lyse tissue samples

– Online SPE system (couples with LCMS)

• Dissolution/Formulation– Dissolution baths, friability and

disintegration instruments• Physico Chemistry

– HT Log P and pKa analyzer– HT oligonucleotides purity

analyzer– HT proteins analyzer– HT DNA analyzer– HT Chiral analyzer– Kinetic solubility instruments

• Service– Agilent Channel Partner to

service their range of HPLC, UV Spectroscopy and CE

• Rapid microbiology with Micro PRO

• Analytical/preparative– OPLC chromatography

solutions for semi preparative applications (OPLC systems, pumps, sample applicator, video imaging and densitometry instruments, reagent sprayer.

– Spotter for MALDI and tissue MALDI imaging

– ELSD detectors– Type-C silica hydride HPLC

columns Flat sorbent beds for OPLC

– Accessories and consumables for CE and HPLC

– Validation kits for HPLC systems

Benefits of HT400E system

• Reliable– Based on reliable technology of HTA autosampler already

used by Agilent, Shimadzu, Perkin etc ... In their GC systems

• Reproducible– Very low CV% inter-assay <2,5%

• Flexible– Accepts many types of SPE cartridge, sample and target

vials

• User friendly– Intuitive “drag-and-drop” software

• Competitive Price– Highest cost-to-performance SPE system available on the

market

HT400E system with cooling unit

Main features of HT400E system

• Can be used as an SPE system or HPLC autosampler

• 20/29 samples max per tray

• 4 solvents available (more on request – max 11)

• Syringe and needle washing

• Software manager QUICK-PREP

• Sample tray Peltier cooling unit (optional)

Sample tray configurations

• 20/29 samples per TRAY

• Sample volume: ∞ ml

• SPE– 1ml , 3ml standard– 6 ml optional

• Target Volume:– 2 ml– 4 ml

• Syringe:– 1 ml– 2.5 ml

• Other on request

Ideal workflow

• Cartridge activation– Volume, n. of solvent, n. of cycles

• Cartridge drying– # of cycles

• Sample loading– Volume, # of cycles

• Sample washing– Volume, # of solvent, # of cycles

• Elution– Volume, # of solvent, # of cycles, different target vials

• Reagent addition– Volume, # of reagent, different target vials

• Washing syringe and needle– # of cycles

• Post-washing cartridge to re-use it

Easy-to-use Quick-Prep software

• Drag-and-drop software– Tools such as Draw, Dispense,

Mixing, Elution, Drying, Cartridge Picking, Cartridge Carry, Injection, Valve Washing, Cartridge Conditioning etc ... can be dragged and dropped in method making it possible to write methods in minutes

• Easy and intuitive software

• Easy set-up of the workstation (WorkTable)

• Easy-write work-up process (WorkFlow)

• Possibility to link methods

Application examples and results

• The following evaluation has been made using the VMA kit of Chromsystems

• The procedure has been as requested by Chromsystems protocol

Chromatogramme of VMA separation

Minutes

0 2 4 6 8 10 12 14

mV

olts

0

100000

200000

300000

400000

500000

600000

mV

olts

0

100000

200000

300000

400000

500000

600000

2,68

7 V

MA

3,68

3 is

o-V

MA

7,24

3

10,4

83 5

-HIA

A

13,8

17 H

VA

ECD - Analog Board 2

Retention TimeName

Reproducibility of VMA separation

Retention Time Area Name ISTD concentration Units

2,685 2177549 VMA 4,670 mg/L

3,680 3811619 iso-VMA 0,000 mg/L

10,478 4300813 5-HIAA 5,066 mg/L

13,837 3194021 HVA 4,826 mg/L

  1 2 3 4 5 6 7 8   AVR. S.D. R.S.D

                         

VMA 4,67 4,87 4,64 4,58 4,92 4,78 4,73 4,67   4,73 0,117 2,47%

5-HIAA 5,05 4,98 4,98 4,72 4,91 4,82 4,81 5,06   4,92 0,123 2,50%

HVA 4,83 4,66 4,87 4,73 4,74 4,73 4,64 4,83   4,75 0,083 1,75%

                         

Carry-over on pathological control

Minutes

0 2 4 6 8 10 12 14

mV

olts

0

200000

400000

600000

800000

mV

olts

0

200000

400000

600000

800000

2,5

85 V

MA

3,5

00 i

so

_V

MA

3,9

95

9,0

50 5

-HIA

A

12

,172 H

VA

13

,043

EC Detector - Analog Board 2

Retention TimeName

Retention Time Area Name ISTD concentration

Units

2,585 6645979 VMA 14,150 mg/L

3,500 3968477 iso_VMA 0,000 mg/L

9,050 9851053 5-HIAA 15,390 mg/L

12,172 7550284 HVA 12,711 mg/L

Pathological Control

Carry-over on water

Retention Time Area Name ISTD concentration

Units

2,585 6645979 VMA 14,150 mg/L

3,500 3968477 iso_VMA 0,000 mg/L

9,050 9851053 5-HIAA 15,390 mg/L

12,172 7550284 HVA 12,711 mg/L

Minutes

0 2 4 6 8 10 12 14

mV

olts

0

100000

200000

300000

400000

500000

mV

olts

0

100000

200000

300000

400000

500000

3,4

97 iso

_V

MA

EC Detector - Analog Board 2

Retention TimeName

WATER

Specifications

HPLC

Sample volume: Fixed loop

Syringe sizes: 0.5ml, 1ml, 2.5ml, 10ml (2.5ml as standard)

Derivatization time: User defined

Derivatization function: Choice of reagents

Needle wash: With solvent/sample

Loop wash: With solvent/sample

HPLC tray option: 110 sample positions

SPE

SPE cartridge volumes available: 1ml, 3ml

Tray capacity: 29 tubes (20 as standard)

Tray temperature control option: Peltier system. 10°C below ambient to +40°C

Solvent lines: Up to 10 (4 as standard), plus one wash solvent

Physical Characteristics

Dimensions (W x H x D): 510 x 735 x 400mm

Mass: 15.0kg

Power: 110/220V AC, 50/60Hz

For more information on HT400E system

• To see a video, click on picture besides in Diaporama mode or copy following URL in your browser

http://www.youtube.com/watch?v=XApeb60kGUs

• To download a brochure click on or copy following URL

HT400E SPE system brochurehttp://wamoyal.free.fr/LabHut/SPE/HT400E%20SPE%20system.pdf

Contact details at Disruptive Technologies

Contact

William Amoyal, Sales Manager

Disruptive Technologies

3 Allée des Camélias

94440 Villecresnes

Mobile: +33 (0)6 98 64 98 81

Email: wamoyal@disruptechno.com

Web: www.disruptechno.com