www.JantDx.com

Automated, Multiplexed Molecular Testing

Infectious Diseases Genetic Screening Pharmacogenetics (PGx) Carrier Predisposition Cancer Genetics

Your System of Choice

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Introducing our new fully automated NanoChip®XL analyzer:

FASTER. SIMPLER. SMARTER.

When the BEST

becomes Better!

The NanoChip®XL analyzer is a novel bench-top molecular microarray system enabling fully automated high throughput testing of DNA/RNA yielding results in the same working day with minimal hands-on time.

Applications include Infectious Diseases, Genetic Screening, Pharmacogenetics, Cancer Genetics and Carrier Predisposition to diseases, enabling medium-high throughput labs to combine all their molecular testing on one simple screening platform.

®XL analyzer:

FastImproved robotics and optical mechanism- faster cartridge initialization allowing for shorter run time

RobustAutomated maintenance procedure and optical normalization

Load & GoPCR On board allows for loading the PCR plate, reagents and cartridge, Pressing START and that’s it…you are all set!

Friendly & Flexible Very simple operation, including

touch screen option Up to 4 different assays in the same

run and on the same cartridge

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It’s as simple as 1,2...

The NanoChip®XL System is an automated platform capable of detecting multiple targets for individual samples, and of analyzing multiple samples on the same electronic microarray with walk-away automation. When using the NanoChip®XL System, molecular diagnostics labs are offered flexibility, cost savings and ease of use in a wide range of applications such as pathogen sequence detection, SNP genotyping and pharmacogenetics testing. CE-IVD approved kits are available for various applications.

The New System

1. Dispense the Master Mix to each well containing the extracted DNA sample.Seal the PCR plate with Microplate seals and load into the NanoChip®XL thermo cycler (PCR block)

2. Load the NanoChip® cartridge and reagents and start the run

Master Mix PCR plate

Reference Reagent Pack NanoChip® Cartridge

Position Sample Dataset pan-sal ipaH cadF tpi tcdA/B EH 18S ß-Gia COWP1 DF 16S Interpretation

1 012-5135 NC-GIP + - - - - - - - - Salmonella

2 012-5136 NC-GIP - + - - - - - - - Shigella

3 012-5137 NC-GIP - - + - - - - - - Campylobacter

4 012-5138 NC-GIP - - - + - - - - - Non toxinogenic C. difficile

5 012-5139 NC-GIP - - - + + - - - - Toxinogenic C. difficile

6 012-5140 NC-GIP - - - - - + - - - E. histolytica

7 012-5141 NC-GIP - - - - - - + - - Giardia

8 012-5142 NC-GIP - - - - - - - + - Cryptosporidium

9 012-5143 NC-GIP - - - - - - - - + D. fragilis

Example of Gastrointestinal Panel results demonstrated

NanoChip® Electronic Microarray Technology

The NanoChip® cartridge is the heart of our technology. It houses a CMOS semiconductor microchip, or “smart chip”, called the NanoChip®.

There are 400 test sites available on the NanoChip®, with each test site individually controlled by 12 electrical connections, which allow users to design and run molecular tests. Additionally, the smart chip technology allows for memory of which test siteshave been previously used.

Each test site consists of the silicon CMOS circuitry, platinum electrode and permeable gel layer. Digital commands are sent to indicate which individual test sites are to be activated by applying a positive or negative charge.

The permeable gel layer covering the chip contains streptavidin, which is utilitzed to non-covalently attach biotinylated DNA molecules (or capture oligos) to the test site. The strength of this streptavidin-biotin bond allows the amplicon to remain attached to the activated test site while excess amplicons are washed away, preventing cross contamination.

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A

T

Biotin

C

G

Biotin

Green Reporter

(Wt Allele)

Biotinylated Capture Probes

Amplified DNA Sample

Red Reporter (Mut Allele)

Example of one Heterozygotesample on one pad

Green Reporter (Pathogen 2)

Red Reporter (Pathogen 1)

Biotinylated Capture Probesfor Pathogen 1

Amplified DNA Sample from Pathogen 1

Example of MultiplexedPathogen ID on a single pad

Biotinylated Capture Probesfor Pathogen 2

Amplified DNA Sample from Pathogen 2

B B

Pathogen

SNP Application

SNP

Pathogen Application

Cat. # Product name Targets Tests/kit

899050Infection Control Panel

• Methicillin-resistant Staphylococcus aureus (MRSA)• Vancomycin-resistant Enterococci (VRE)• Carbapenemase - producing klebsiella pneumoniae (KPC)

96

899054 MRSA Panel spa & nuc • mecA • SCCmec 131

899061 Clostridium difficile Panel gdh • tcdA/tcdB 192

899051Gastrointestinal Panel Combi I

• Salmonella • STEC• Shigella • Cryptosporidium spp.• Campylobacter • Entamoeba histolytica• C. difficile (+Toxin) • Giardia lamblia

78

899057Gastrointestinal Panel Combi II

• Salmonella • Entamoeba histolytica• Shigella • Entamoeba dispar• Campylobacter • Dientamoeba fragilis• Giardia lamblia • Blastocystis spp.• Cryptosporidium spp.

96

899058*Gastrointestinal Panel Combi III

• Salmonella • Giardia lamblia• Shigella • Cryptosporidium spp.• Campylobacter • Entamoeba histolytica• STEC • Dientamoeba fragilis• Yersinia enterocolitica • Blastocystis spp.

78

899052Gastrointestinal Parasites Panel I

• Giardia lamblia • Entamoeba dispar• Cryptosporidium spp. • Dientamoeba fragilis• Entamoeba histolytica • Blastocystis spp.

131

899053*Gastrointestinal Bacteria Panel I

• Salmonella • Yersinia enterocolitica• Shigella • STEC• Campylobacter

131

899055Sexually Transmitted Infections Panel STI Plex

• C. trachomatis• N. gonorrhea• T. vaginalis

192

899056Sexually Transmitted Infections Panel STI Plex+

• C. trachomatis • N. gonorrhea • T. vaginalis• M. hominis

131

899017 HPV Screening Panel

• Genotyping of HPV High risk 16, 18• Identification of HPV High risk 26, 31, 33, 35, 39, 45, 51, 52,

53, 56, 58, 59, 66, 73, 68, 82• Identification of HPV Low risk 6 and 11

192

899019* HPV Genotyping Panel

• Genotyping of HPV High risk 16, 18, 26, 31, 33, 35, 39, 45,51, 52, 53, 56, 58, 59, 66, 73, 68, 82

• Genotyping of HPV Low risk 6, 11, 40, 42, 43,44, 54, 55, 61, 62, 67, 69, 70, 71,72, 81, 84,89

192

899062*Immunocompromised Respiratory panel

• Influenza A/B • HSV• Parainfluenza 1, 2, 3 • Legionella spp.• RSV A/B • Aspergillus spp.• Adenovirus • Pneumocystis jirovecii• CMV

96

Molecular Diagnostics

HA

I

- Infectious Diseases

GA

STR

OE

NTR

ITIS

STI

HP

VR

espi

rato

ry

• M. genitalium• U. urealyticum• U. parvum

* CE in-process

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Cat. # Product name Disease Tests/kit800272 CF14 Cystic Fibrosis (CFTR); 14 SNP's 64/128

800001 CF19 Cystic Fibrosis (CFTR); 19 SNP's 64/128

803001 CF70 Cystic Fibrosis (CFTR); 70 SNP's 76

800372AJP-12(Ashkenazi Jewish Panel)

Alpha 1 Antitripsin (PI) • Bloom Syndrome (BLM) • Fanconi Anemia C (FACC) • Familial Dysautonomia (FD) • Glycogen Storage Type 1a (GSD) • Mucolipidosis IV (ML4) • Maple Syrup Disease (MSUD) • NiemannPick Type A,B (NP) • Usher Syndrome (US) • Canavan (ASPA) • Nemaline • Joubert; 18 SNP’s

64/128

800472 Connexin Hereditary Hearing Loss; 3 SNP’s 96/192

800572 Tay Sachs Tay Sachs (HEXA); 6 SNP’s 96/192

800672 Gaucher Gaucher Type 1 (GBA); 7 SNP’s 96/192

800014 All-In-One 27 Diseases; 92 SNP’s 21

800018 Beduins Panel 9 Diseases; 9 SNP’s 131

Cat. # Product name Disease Tests/kit700103 FMF-12 Familial Mediterranean Fever; 12 SNP’s 96

899015 CVP5 Cardio Vascular Panel; 5 SNP’s 133

800016 BRCA-19 Breast Cancer; BRCA1/2; 19 SNP’s 64

Cat. # Product name Disease Tests/kit899010* CYP2C9/VKORC1 Cytochrome 450 64

899011* CYP2D6 Cytochrome 450 64

899012* CYP2C19 Cytochrome 450 64

- Genetic Carriers Screening

- Carriers Predisposition to Disease

Pharmacogenetics

* Under Development

Please contact us for detailedSNP's/targets lists.

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The SystemInstrument Specifications:

Instrument Dimensions:Height: 0.56 mDepth: 0.66 mWidth: 0.90 m

Instrument Weight: 105 kg

Voltage Rating: 100-120 or 220-240 VAC, 50/60 HzPower capacity: 650WFuse: 250 VAC / 5A

Environmental Restrictions:Operating Ambient Temp: 18°C to 30°COperating Ambient Humidity: 40 to 80%Altitude: up to 2000 m

Optics: Illumination Information:Red LED: Excitation Wavelength = 626 nmGreen LED: Excitation Wavelength = 525 nmEmission wavelength: 553 nm (green), 668 nm (red)Filters: Red, Green, Neutral Density

Detection: CCD Camera and Board

Computer Specifications: Portwell PPC, Core 2 Dou processor 17 inch flat touch screen display

The NanoChip®XL system is an automated platform for the detection of multiple targets across multiple applications

NanoChip®XL System NG-M800XL

400-site Cartridge NG-S800160

Fluidics Cartridge (4/pack) NG-S800161

Cartridge Specifications:

Assay Cartridge: Length: 104 mmWidth: 38 mmHeight: 15 mmLaminar-Flow Fluidic ChannelsSealed Fluidic Channels400-Site Microarray with CMOS LogicThermally Calibrated for Temperature ControlPermeation Layer Coating for DNA/RNA BindingCartridge-Tracking Barcode Label

Environmental Restrictions: Operating Temperature: 18-60°CStorage Temperature: 2-8°C

General purpose reagents needed in order to run the various assays

Low Salt Buffer NG-S800154

High Salt Buffer NG-S800155

Target Prep Buffer NG-S800156

NanoChip® Microplate

Seals (100/pack)

NG-S800061

Distributed by: Jant Pharmacal Corporation16530 Ventura Boulevard, Suite 512, Encino CA 91436 800.676.5565 818.986.8530 Fax 818.986.0235 i n f o @ a c c u t e s t . n e t

www.JantDx.comFor Research Use Only

NanoChip is a registered trademarks of Gamida B.V, The Netherlands.The cartridge and NanoChip 400 system and their use may be covered by one or more of following U.S. Patents or Applications: 5,605,662; 5,929,208; 6,017,696; 6,238,624; 5,632,957; 6,582,660; 5,849,486; 6,245,508; 6,048,690; 6,051,380; 6,518,022; 2003/0190632; 5,965,452; 6,258,606; 6,682,936; 6,687,048; 2002/0131899A1; 2004/0149582A1; 6,099,803; 6,726,880; 6,225,059; 6,068,818; 6,540,961; 6,254,827; 6,315,953; 6,821,729; 6,331,274; US-2004-0038420-A1; 6,306,348; 6,524,517; 6,303,082; 6,838,053; 2005/0158451A1; 6,960,298; 2005/0164283A1; 6,207,373; 6,395,493; 6,753,148; 6,468,742; 2003/0073122; 6,379,897; 6,492,122.

Please note: Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser›s own human in vitro diagnostics other than internal research and development and applied fields under non-real-time thermal cycler patents of Applied Biosystems LLC