www.curiosis.com

Automated live cell imaging system

Celloger Mini

Instruction manual

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Developed and manufactured by CURIOSIS Inc. Email: info@curiosis.com Website: www.curiosis.com

CURIOSIS Inc. 4F, 10 Teheran-ro 38-gil, Gangnam-gu, Seoul 06221, South Korea Tel: +82-2-508-5237 Fax: +82-2-508-5246

Copyright © 2021, by CURIOSIS Inc. All rights reserved. Published in Korea.

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Table of contents

Package contents

Device layout

Getting started Installation

Network setup

Control panel guide

Basic operation 1. Device connection

2. Cell monitoring (Setup mode)

3. Details

Focusing

Manual adjustment

Autofocusing

Plate panel

Features on display

Time-lapse imaging 1. Scan position setup (Setup mode) 2. Scanning (Scan mode)

File storage and project name Time setup

3. View image file (Album mode) 4. Movie maker (Album mode)

Image analysis (Analysis mode) 1. Confluency 2. Growth curve

Safety information General guidelines Operating condition Safety standards

Cleaning and Maintenance Trouble shooting Product specifications Ordering information Appendix A. Connecting multiple devices Appendix B. How to insert Vessel or Vessel Holder Appendix C. Correct focus and light intensity selection

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5

6

13 13

14

17

20 20

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21

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23 23

24

25

26

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29

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30

32

33

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Package contents

Celloger Mini Live cell imaging device package includes the following items.

Item Quantity

CELLOGERTM MINI main device 1

Main power cable 1

PoE 1

LAN Cable (5M, 4.8M) 2

USB memory 1

-Quick manual 1

-IP Setting guide 1

-Celloger App 1

Vessel holder for dish 60mm (Optional) 1

Vessel holder for dish 35mm (Optional) 1

Vessel holder for T-flask 25cm2 (Optional) 1

When receiving the package,

• Check that all items listed above are included in your package.

• Examine the device carefully for any damage during shipping.

• Contact your local distributor or info@curiosis.com if any items are missing or damaged.

• Any loss or damage claims must be filed with the carrier.

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Device layout

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Getting started

Installation 1. Remove the product from the box.

When taking out the device from the box, ensure to hold the bottom of the device. Brightfield lamp is located at the center of the device and it is not a handle.

2. Take off the lens cover (black).

3-1. Connect to power cable (Previous version).

A. Plug the power cable into device and connect to PoE (P+ DATA OUT / Right side)

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B. Connect the device to PC using the connection cable and PoE (DATA IN / Left side)

3-2. Connect to power cable (Current version). A. Plug the power cable into PoE

B. Connect the device to PC using the connection cable and PoE

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Network setup 1. Click the “Start” button or the “Window” icon. 2. Click the “Setting” button. 3. Click the “Network & Internet” button. 4. Click the “Ethernet” button. 5. Click the “Network and Sharing Center” button. 6. Click the “Change adapter settings” button on the left menu. 7. Click the “Ethernet” button and right-click the mouse and click “Properties” in the window that appears. 8. Select “Internet Protocol Version 4 (TCP/IPv4)” and click “Properties”. 9. Select “Use the following IP address” and input “IP address” and “Subnet mask”. Note: “IP address” should be 192.168.2.##, and in ## field, input 2~254 except 10.

For “Subnet mask”, input 255.255.255.0.

The PC IP address should be set differently from the device IP address.

10. Click “OK”.

<Image Descriptions >

①

②

③

④ ⑤

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⑥

⑦

⑧ ⑨

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Control panel guide

① APP version : Application version of the software

② Start : Device connection

③ Status (Condition or Status)

: Preview : Scanning is underway

④ Frame Resolution

✓ Normal : 1296 x 970 pixel resolution. Used for general time-lapse imaging. ✓ High : 2592 x 1942 pixel resolution. Used for a single capture. Able to get a clearer image than

Normal resolution.

Caution! : To obtain the confluency data, you must capture or scan images using Normal. High does not perform analysis; it is only possible to analysis an image that is Normal resolution.

⑤

④

⑭

②

Lamp : Meaning that currently lamp is brightfield.

Objective Lens : Meaning that lens magnification is 4x.

③ ⑧

⑨

④ ⑩

⑪

⑥

⑦

⑫

⑭ ⑬ ②

⑤

⑯

⑮

①

③

○17 ⑱

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⑥ Image information ✓ Pixel Info(X,Y) : It shows the pixel intensity at the mouse cursor location (X,Y) on display.

Intensity ranges from 0 to 255. 1) Int : The value of intensity at the mouse cursor location on display. Higher intensity means

bright and lower intensity means dark. 2) Avg : Average of all intensity values on display

✓ Image Size : Total number of pixels ✓ Zoom : present magnification

⑦ Scale bar : A visual indication of distance and feature size (page 19)

⑧ Menu bar

✓ Setup mode: Position, focus, vessel type, Image format, autofocusing setting can be adjusted before scanning. ✓ Scan mode : It is a mode to set schedule for time-lapse imaging, showing the progress of time-lapse. ✓ Album mode : Mode to show the files taken with Celloger Mini and video can be made. ✓ Analysis mode : It calculates the confluence of images taken by Celloger Mini and growth curves can be drawn

for time-lapse images.

⑨ X-Y-Z position : It shows the current coordinates of X, Y, Z

⑩ Imaging control panel

⑪ AutoFocus : Execute autofocusing depending on the autofocus settings in streaming status (refer to page 18 for autofocus setting).

⑫

⑬ Preview ✓ Start Streaming : Preview on

✓ Stop Streaming : Preview off

⑭ Temperature & Humidity : Thermometer/hygrometer next to the LED light source in the bright field. Note: The temperature and humidity sensors of Celloger Mini are not calibrated and used only for the purpose of checking changes in value. If there is thermometer/hygrometer and calibration is required, contact Curiosis (via e-mail) to request calibration.

⑮ Blink me! : Upon pressing Blink me!, the LED indicator lights in white. Useful upon using multiple devices.

Lamp power : LED intensity

Brightness : Brightness of a camera

Contrast : Contrast between bright part and dark part

Measure : Measure the length of the desired part (page 18)

Select Layer : Composed of “BR”(brightfield image), “Cell”(confluency mark)(page 18)

Reset Zoom & Pan : Go back to Zoom and Pan state before adjustment

Temperature

Humidity

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Exchange : Function to make the stage come out of the device for easy mounting of vessel or vessel adapter. ⑯

✓ Connection status: Showing device connection status ✓ Device IP address : IP address of connected device ✓ Device version : Firmware version of connected device

○17 Display: It shows live or captured images.

⑱ Mode setting panel: Section changes depending on mode.

Device

IP address

Device

version

Connection

status

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Basic operation

1. Device connection 1. Run CellogerMiniApp. 2. After a window pops up, select the icon of the device to be connected in Connect Device and click OK and then the

device is connected.

Note : If the device icon is not shown on the window, please check the followings.

① Check the PC IP address setting. (page 8)

② In case of multiple devices, refer to APPx A. (page 30)

③ When Adapter is connected, select Network Adapter and select connected adapter by pressing an arrow.

①

②

① ②

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2. Cell monitoring [Positioning (position selection) and light intensity control] 1. Put the device in the incubator. 2. When the stage comes out by pressing , install the vessel. (In case of dishes or flasks, install the vessel

adapter first, then the vessel. Refer to Appx B for how to insert a vessel or vessel adapter (page 32). Upon installing

well plate, make sure that A1 is on the left front.

3. Select the vessel type in Setup mode. Well plate, dish, flask can be selected. Upon selecting vessel type, the vessel

type is displayed on the plate panel at the bottom right.

Note : A vessel adapter suitable for vessel type must be used. (use vessel adapter for vessels except for well plate)

4. Click and move desired vessel location on the plate panel with the mouse. (Upon clicking vessel or well in Plate

panel, it moves to center of vessel or well). 5. Upon clicking Start Streaming, a real-time image is shown on the display. [If the screen is black, light intensity

should be adjusted first (step 8)].

Plate panel

A1

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6. Use Jog or Manual position to adjust the position precisely. Rough adjustment is possible with Plate panel (page 17)

① Jog : Move to the center of the stage. : Move left and right. Using arrow button on the keyboard to move step by step. : Move up and down. Using arrow button on the keyboard to move step by step.

: Use the scroll bar to move left and right for rough adjustment.

: Use the scroll bar to move up and down for rough adjustment. : Stop the movement of stage

* It is possible to adjust motor speed and step distance.

Speed control: On the left circular slider, move the green arrow to change the value at the center of the circle (can select the value from 10 to 100%) Step control: On the right circular slider, move the blue arrow to change the value at the center and adjust the unit after opening combo box (alternative keyboard “ * ”, “ / ”) (1 um ~ 10 mm)

Note : If the speed is set at value exceeding 30%, there is a risk of device malfunction so setting the value less

than 30% is recommended.

② Manual Position After inputting the desired location in X, Y, Z and press Go, the location is changed.

①

②

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7. Adjust focus manually or using AutoFocus . Autofocus works depending on the AF set range so perform this job after adjusting AF with Jog (page 17). 8. On the Imaging control panel, adjust LED intensity, brightness and contrast by using scroll bar.

✓ Lamp power : Adjustable from 0 to 255

✓ Brightness : Adjustable from -2048 to 2047

✓ Contrast : Adjustable from 0 to 160

Note : The recommended values are as follows. Lamp power: 135, Brightness: 256, Contrast: 40. The higher the difference between cell and background, the better calculation of confluency (page 33).

9. Select Image Format and Frame Resolution in Imaging. Frame Resolution can also be selected on the left side of the display. 10. To save the image, right-click on the display and click Save As. The saved images can be seen in ScreenCaptured file. Two image files, one with raw image and the other one with feature are generated.

<Raw image> < Image with feature >

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3. Details

Focusing ➢ Manual adjustment Adjust focus in Jog by using . Possible to adjust focus by using “ + ”, “ - ” on the number pad of the keyboard. ➢ Auto focusing The Celloger’s Autofocus algorithm scans within a certain depth and selects the area where cells are clearly shown. Therefore, location should be set manually first and execute Autofocusing function. 1. Set the rough focus manually. 2. Adjust focus and press AutoFocus.

Note : In the AF panel in Setup mode, autofocusing can be set.

✓ Depth [mm] : Range of AutoFocus fuction ✓ Resolution [mm] : Scanning unit within depth ✓ ROI Section : The area can be selected and AutoFocus

algorithm is executed based on the selected area.

Plate panel Possible to set scanning order by right clicking the mouse on Plate panel. The table on the left side of the Plate panel shows Scan Position Set.

✓ Move to center position : Move to center position : Upon

clicking vessel on plate panel, it moves to the center.

✓ Move to mouse position : Upon clicking the desired location in the vessel on Ctrl + plate panel, it moves to the location clicked.

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✓ Is show ‘No’ : If it is checked, scanning order is displayed on the plate panel and if it is not selected, the number disappears.

✓ Is snap to well : After selecting this, it is possible to move from one well to another well using arrow keys on the keyboard.

✓ Scan direction : It shows the scanning order if all wells are

selected on the well plate. One Way scans in the

direction and Two Way scans in the direction. ✓ Set all centers : Set position is possible for centers of all

wells on the well plate.

✓ No : Scanning sequence ✓ X, Y, Z : Each coordinate ✓ Label : Well number ✓ AF : Display whether autofocusing is run on Time-lapse imaging or not.

✓ : Able to delete all scan positions designated To delete just one

scan position, select the location to be deleted and press Delete key on the keyboard.

✓ : Import the exported scan position table.

✓ : Save scan position table.

Features on display Display features of the Display screen. ➢ Measure, confluency mark (using icon located at the right bottom of Display)

① Measure Select one of diagonal, horizontal and vertical directions, obtain data by clicking one end to the other end of the area to be measured.

② BR : Streaming image or captured image layer Cell : It is the layer that shows the surface covered by adherent cell in colors when ‘run’ is pressed in the analysis mode. (refer to confluency analysis and color setting page 23)

①

BR Cell

②

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➢ Zoom, Pan, Capture, Scale bar, Histogram, Center mark, Video area Grid line (Using right-click of the mouse on Display)

✓ Reset Zoom & Pan : Reset after using Zoom, Pan functions ✓ Zoom : Click Ctrl + Mouse wheel to magnify images ✓ Pan : Click Shift + Mouse Drag to move images ✓ Save As… : Save images (raw image and captured image with features are

saved.) ✓ Stage Position : Show current position on the right top of Display ✓ Scale Bar : Show scale bar on the left bottom of Display ✓ Histogram : Display Intensity Histogram of images

✓ Center Mark : Display the reference point at the center of display in cross

mark

✓ Video Area : Area shown in the video upon making video files.

✓ Grid Lines : Draw grid line on Display and possible to change the number

of grid lines.

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Time-lapse imaging

1. Scan position setup (Setup mode)

1. Place the device into an incubator. 2. Mount vessel when the stages comes to the front after pressing . (Install vessel after installing vessel

adapter first in case of dish or flask.) 3. Select Vessel type and desired location on the plate panel. 4. Press Start Streaming. 5. Move to the desired location in the Setup mode. (page 15 step 6) 6. Adjust focus with Jog or Autofocus. (page 17) 7. Adjust brightness, contrast or lamp power, if necessary. (page 16 step 8) 8. Select Image Format and Frame Resolution on Imaging.

9. Designate the position by pressing on desired location. After designation, designated location is shown on the plate panel and scan position set (page 17 for more details about Plate panel and Scan position set).

Note : Note : If Auto Focus is checked in and Set Position is executed, autofocusing function is executed automatically. Align Image is location correction function. It corrects location if the location is stepped out compared with the previous cycle during scanning. However, if interval time is 6 hours or longer or changes in morphology are extreme within a short period of time, differences in image from previous cycle becomes large, having a negative impact so in this case, it is better to turn off the setting.

2. Scanning (Scan mode)

Designation of file storage location and project name In Scan mode, set the location (location where files will be saved) and project name. In the Location file, folder is created in the name of project name and date set. If location designation is skipped, the file is saved in the scan file and project name is not designated, folder name is created with the start time. Folder is created for each Set Position.

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Time setup 1. Set total imaging time and interval. Minimum interval is 1 minute and total time can be set for up to 60 days.

(HH:MM -> Hour:Minute, DD:HH:MM -> Day:Hour:Minute) Check whether storage is enough before starting scanning.

Important! It is recommended to set recommended interval time below depending on the number of scan position. If it is not complied with, condensation and stepping out may occur.

2. Start scan by pressing Start. To suspend scan for a while, press Pause. Press Pause again to restart. Press Stop to stop scanning.

✓ Total : Scan progress ✓ Cycle : Scanned set position/total set position ✓ Cycle Time Remaining : Time remaining until the start of

the next cycle ✓ Elapsed Time : Time since the scanning starts

✓ Start Date : Date and time of starting scanning

✓ End Date : Date and time of ending scanning

Important! Input the equipment and plate into an incubator for more than 30 minutes for warming up to prevent condensation before starting time-lapse.

3. View image file (Album mode) ➢ How to view in Album mode

1. Select Album mode.

2. Select on the right side of the screen. 3. Select the desired folder. Upon opening a folder, the images in the folder are sorted, and upon selecting it, it is

displayed on the display.

Position Recommended interval time

6-12 >10 min

12-48 >30 min

96 >1 hour

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➢ How to open the file directly Select the Scan file in the CellogerApp folder or designated folder and go into project name folder. You will find the images saved every cycle by Set position.

4. Movie maker (Album mode) 1. Select Album mode.

2. Select on the right side of the screen. Select the Scan folder or folder where you want to create videos. Upon opening the selected folder, the images in the folder are listed in the order of cycle on the right. The higher level folder shows Set position and lower level folder shows the images in the selected folder.

Note : To make a video file, data should be obtained from CellogerMiniApp.

3. Adjust Crop ratio and FPS. Crop ratio means video area and FPS means frame per second.

Note : It is recommended to set Crop ratio at 10 or higher.

4. Press Play button to check time-lapse images that are displayed consecutively from the point

when the button is pressed. Upon pressing Stop button, it is stopped.

5. Check the higher-level folder and press the Record button to save the video file. The file is automatically saved in the location of the higher level folder.

Note : Check the speed by opening the video file after recording as the speed of video is different on Play and Record.

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Image analysis

1. Confluency (Analysis mode)

1. Select Analysis mode. 2. On Source, select one from Current Image, Local Image, Time-Lapse Images.

✓ Current Image : Image that is shown on display now ✓ Local Image : Select image from images saved in a

computer ✓ Time-Lapse Images : Select a desired file among files

scanned in CellogerMiniApp.

3. Adjust parameters in Color.

Note : The alpha button controls the transparency or opacity of a color.

4. Conduct confluency analysis by pressing Run. 5. The green part below is confluency (%) and the area of cells analyzed is shown as cell mark.

(To turn off the cell mark, in , press for cancellation.)

<Result>

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Note : It is possible to see the image of the cycle you want by double clicking in the Time-Lapse Images, but cell mark does not follow.

2. Growth curve

1. Select Analysis mode. 2. On Source, select Time-Lapse Images and open the file from which you want to get the result. 3. Control parameters on Color. 4. Press Run to obtain confluency(%). It is possible to show confluency[%] in Data every cycle and obtain growth

curve on Graph.

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Safety information General guidelines

Install the device on a rigid and level places.

Operate the device in conditions described in the operating condition.

Use only the components provided and authorized by Curiosis.

Ensure the input voltage matches with the device’s power supply voltage.

Check if the power cable is properly grounded to avoid potential electric shock.

Disconnect the power cable when abnormalities occur.

Wait about 2-3 minutes for the device to boot.

Do not insert any metallic objects into the device through bottom and side air vent to avoid electrical shock causing personal injury or device damage.

Place the device around 10cm away from the surroundings for proper air-cooling.

Do not disassemble the device in any event. Contact your local distributor to arrange for service in case of malfunctioning.

Operate the device carefully as described in this manual.

Operating condition

Operating power 100~240 VAC

Electrical input 24 VDC, 2.5 A

Frequency ~50/60Hz

Operating temperature 10~40℃

Maximum relative humidity 20~95%

Installation site Indoor user only

Safety standards

European standards US standards

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Cleaning and Maintenance

When cleaning optical elements, use only a damp cloth to avoid scratching soft lens coatings.

Lightly wipe working surfaces of the CellogerMini with soft cloth dampened with 70 % ethanol or hydrogen peroxide(H2O2). Do not pour or spray liquids directly anywhere on the instrument.

If liquid spills on the instrument, turn off the power and wipe dry immediately.

In case of storage at a room temperature after taking it out of the incubator, dry it first in a dry and well-ventilated place and store it.

As the device uses X-Y-Z axis motor, it is sensitive to vibration and horizontality. It should be used in a flat place without vibration.

Never disassemble the instrument yourself. Do not remove any covers or parts that require the use of a tool to obtain access to moving parts.

Operators must be trained before being allowed to perform the hazardous operation.

Unauthorized repairs may damage the instrument or alter its functionality, which may void your warranty. Contact your local distributor to arrange for service.

Do not place the POE injector inside in the incubator.

Do not block the fan hole of the device.

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Trouble shooting Installation Device does not power up

✓ Check power source or contact your distributor. ✓ Replace power cable if it is in poor condition.

Cannot connect with CellogerMini device ✓ Make sure the device is connected to the computer. ✓ Unplug and re-connect all cables. Restart the CellogerMiniApp program.

Focus Focus is irregular

✓ Reload the vessel, so that it lies flat on the stage. Be sure vessel holder is mounted flat with respect to stage. ✓ Make sure the sample thickness is uniform.

Autofocusing does not work properly ✓ Before using Autofocusing, partially set the focus manually. ✓ Change the Autofocusing setting.

Image monitoring Image display is dark

✓ Click the Start Streaming button. ✓ Place sample on the Center of objective lens.

Image is not clear ✓ Carefully wipe off the objective lens with cotton swab. ✓ Eliminate any dust on culture dish and LED lamp. ✓ Remove any condensation on the lid of the culture dish.

Problem in saving files ✓ Check the unused volume of the hard disk.

Time-lapse images become dark and bright ✓ Warm up the instrument 30 minutes before monitoring. ✓ Eliminate dust on culture dish. ✓ Check for and remove any condensation on the lid of the culture dish. (In order to prevent condensation from

occurring, it is better to warm up the plate or dish lid slightly upon preparing the sample). ✓ To prevent any problem such as shaking of culture dish or inflowing light, pay special attention when you open

or close incubator door during monitoring. ✓ Check if the recommended Interval time and motor speed are complied with. ✓ Do not install the device close to the front/ door area of the incubator. Given that the incubator has high

temperature and humidity, the area that comes in frequent contact with cool outside air may have condensation. It is recommended to install it inside the incubator as much as possible.

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Data Cannot play the movie

✓ Video can be made only for time-lapse images obtained from Celloger Mini program.

Cannot analyze the confluency ✓ Check the images are obtained from CellogerMiniApp program; Analysis can be made only for images

obtained from CellogerMiniApp. ✓ Before analyzing, check if the focus is clear. ✓ Check if the resolution of images is in Normal; it is only possible to analyze an image that is in Normal

resolution. When a video is made, it is played as if cells are shaking a lot.

✓ If the interval time is too short, the images out of the set location may be taken due to malfunction of the device. Refer to recommended minimum interval time (page 21)

✓ If the interval time is long and align image is set, align image correction function may not work properly. Reduce interval time or cancel align image setting. (page 21)

Others The values in Temperature & Humidity of Celloger Mini are different from those of incubator or separately installed thermometer and hydrometer.

✓ The temperature and humidity sensors of Celloger Mini are not calibrated and used only for the purpose of checking changes in value. If there is thermometer/hydrometer and calibration is required, contact Curiosis (via e-mail) to request correction.

✓ The thermometer/hygrometer are located next to LED light source of Celloger Mini. If this area is clogged, correct measurement is not possible.

<High resolution result> <Normal resolution result>

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Product specifications Dimension 195 x 305 x 220 mm

Weight 4.5kg / 9.9lb

Objective Lens 4X

Imaging modes Brightfield

Camera 1.25MP / 5MP CMOS

Resolution 1296 x 970 / 2592 x 1942 pixel

Stage Motorized XYZ

Imaging positions Multiple

File export format TIFF, JPEG, PNG, AVI

Culture vessels Flask, dish, well plate, slide

Operating environment 5~40℃, 20~95% humidity

Power requirements 100-240V, ~50/60Hz

Output ports Ethernet

Computer External PC

O/S required Window 10

Processor (recommended) CPU 3G

Storage (recommended) 1TB

Monitor (Recommended) 1920 x 1080

Accessories Vessel holders (T-flask A25, Petri dish 35mm, Petri dish 60mm) PoE adapter, ethernet cable, USB memory

Warranty 1 year

Ordering information Catalog No. Product Name Description

CRCLG-MB01 CELLOGER Mini Live cell imaging system (Bright Field)

CRCLG-MBTF25 Vessel holder T-Flask A25cm2

CRCLG-MBPD35 Vessel holder Petri dish 35mm

CRCLG-MBPD60 Vessel holder Petri dish 60mm

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Appendix A. Connecting multiple devices

Curiosis assigns the same IP address to Celloger Mini. Therefore, when connecting multiple devices in one PC, device IP

change is necessary. In addition, switching hub or router with multiple LAN ports are necessary.

When multiple devices with the same IP address is connected to a PC and only one icon appears on Connect Device,

change the IP address of devices except for one device (Make sure that the same IP address of multiple devices is not

entered and device IP address is set differently from PC IP address.)

1. Select Device icon and double click the Device IP address at the right bottom of the screen to open Device

Status window.

Celloger Mini

PoE Injector

PoE Injector

Switch

PC

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2. Click Device IP and Read to bring up the Device IP.

3. Change the IP Address to 192.168.2.## and press Change. Put the numbers from 2 to 254 except for 10 in ##.

Note : If connection with PC is not possible because Device IP

address is lost, there is no way to know the IP address. In case

of changing address, changed IP address should be written

down on a separate sheet.

4. Turn off the device and then turn it on again.

5. Run CellogerMiniApp to check whether IP address has changed.

Note: The device currently connected and running can be identified by clicking or checking the Device IP

address at the bottom of the screen.

<Example>

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Appendix B. How to insert Vessel or Vessel Holder Select the adapter suitable for the vessel type. As shown in the figure below, tilt and insert the adapter or well plate. At this time, insert the area where it contacts with a ball plunger and insert it on the other side. Turn the knob upon inserting vessel into a vessel adapter or removing it from a vessel adapter.

Turn

Turn

Ball plunger

(located inside)

Area where it contacts with

a ball plunger Adapter or

Well plate

< Picture of inserting an adapter in a tilted state > < Picture showing complete mounting of an adapter >

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Appendix C. Correct focus and light intensity selection <1> When it is in focus: Most of the cells look clearly.

Raw image Image with cell mark <2> Out of focus: Some cells are difficult to see as they are separated from the background and are not visible.

Raw image Image with cell mark

<3> Wrong light intensity selection <4> Correct light intensity selection

Curiosis Inc.

HEADQUARTERS

4F, 10 Teheran-ro 38-gil Gangnam-gu, Seoul 06221

South Korea

TEL +82-2-508-5236 FAX +82-2-508-5246

Email: info@curiosis.com

FACTORY

400 Wonam-ro, Namsa-myeon Cheoin-gu, Yongin-si, Gyeonggi-do 17123

South Korea

TEL +82-31-339-6404 FAX +82-31-339-6409

www.curiosis.com