Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format –...

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Transcript of Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format –...

Page 1: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)
Page 2: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

Automated Immunoassays

• Multi-test immunoassays are the most widely used rapid test format – worldwide– ELISA / EIA (Assurance EIA, TECRA)– ELFA (Vidas)

• Benefits– Low cost/test– Low labor cost with automation– Capable of high throughput– Validated, proven technology

Page 3: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

Full automation of EIA procedure

Sample dispensing

Reagent delivery

Washing

Incubation

Photometric reading

Results interpretation

BioControl EIA AutomationBioControl EIA Automation

Page 4: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

Continuous Loading

Bi-directional LIMS Compatibility

High Throughput Up to 186 samples and controls per run Continuous loading

Highly adaptable open platform• Pre-programmed for TRANSIA® PLATE• Pre-programmed for Assurance EIA®

• Compatible with all EIA assays

BioControl EIA Automation BioControl EIA Automation OptionsOptions

Page 5: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

• BioControl EIA’s

Final Enrichment

Transfer 1 mL to Cluster tubes

Inactivationheating block

Load tubes and ReagentOn Gemini

• Vidas

Final Enrichment

Transfer 0.5 mL toVidas Test Strip

InactivationHeating Block

Load Test Strips andSPRs on Vidas

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Page 6: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

Comparison of Through-put

Throughput of 1 Gemini = 3 Vidas Systems

  Gemini Vidas

8 h 480 samples 168 - 280 samples

24 h 1600 samples 560 - 896 samples

Page 7: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

Gemini vs VidasCapability Gemini Vidas Winner

Max Capacity 288 samples / controls

30 samples / controls

Gemini

Continuous Loading Yes No Gemini

Multiple Assays per Run Yes Yes – limited by capacity

Gemini

Hands-on Time Minimized by high sample

capacity

Increased by low sample

capacity

Gemini

Individual Run Time Approx 2 hr Approx 1 hr Vidas

Overall Run Time Minimized by high sample

capacity

Increased by low sample

capacity

Gemini

Calibration 1 / year 28 / year Gemini

Barcode Reader Yes Yes Equivalent

LIMS Capability Yes Yes Equivalent

Page 8: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

• Automates each and every step

WASH

Washer • 8 channels • Aspirate / dispense needles• 3 wash buffers • Customized wash patterns

PIPET

Pipettor • Disposable tips• Capacitive liquid level detection• Pipetting pressure monitoring• 300 µL tips (10-300 µL) – Samples• 1100 µL tips (301-1000 µL) - Reagents

Page 9: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

• Automates each and every step

INCUBATE

2 incubator chambers • Independently controlled• Shaking capacity• Temperatures up to 45°C

3 ambient temperature positions

Unique modular design •Easy service & Maintenance•Minimizes down time•Reduces repair costs

READ

Absorbance reader • 400-700 nm wavelength• Single or dual wavelength• Range 0.0 – 3.0 OD

Page 10: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

• On-board Computer

Computer Ports • 3 USB ports for external peripheral

keyboard, mouse, printer, etc…• VGA monitor port • LAN cable connection• PS2 port

Touch-Screen Interface• Full function user interface

• Automates each and every step and MORE!

Page 11: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

Multiple Assays• Same plate –common protocol• Different plates – common protocol or

different protocols

Continuous loading of resources• Plates and tips• Samples, and reagents• Instrument will show when Plate 3 can be loaded

Continuous operation• Initiate new assays during run

• Flexible to meet your high throughput needs

Page 12: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

Accepts individual sample tubes or cluster tubes

12 loading bay lanes• Accepts both sample and reagent racks

Accepts reagent bottles directly from kit

• No reagent transfer

Page 13: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

Automated resource tracking• Reagent volume tracking• Disposable tip tracking

Resource verification• Are reagents present • Sufficient reagent volume• Sufficient tips

Automatic schedule optimization• Multiple plates / assays• Addition of new samples/ assays

Page 14: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)
Page 15: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

Assurance EIA Pathogen TestsAssurance EIA Pathogen Tests

Page 16: Automated Immunoassays Multi-test immunoassays are the most widely used rapid test format – worldwide – ELISA / EIA (Assurance EIA, TECRA) – ELFA (Vidas)

• TRANSIA® PLATE Salmonella Gold

• TRANSIA® PLATE Listeria

• TRANSIA® PLATEListeria

monocytogenes• TRANSIA® PLATE

Staphylococcal Enterotoxins

TRANSIA PLATE EIA Pathogen TRANSIA PLATE EIA Pathogen TestsTests

*TRANSIA® is also available for Allergen and Chemical Residue detection