Measurement, analysis and prediction of UV filter absorption into the stratum corneum
Atopic eczema: The role of stratum corneum lipids in the ...
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The handle http://hdl.handle.net/1887/20911 holds various files of this Leiden University dissertation. Author: Janssens, Michelle Title: Atopic eczema : the role of stratum corneum lipids in the skin barrier Issue Date: 2013-05-30
Atopic eczema:
The role of stratum corneum lipids in
the skin barrier
Michelle Janssens
The investigations described in this thesis have been co-supervised by Dr. P.J. Caspers and Dr. A.P.M. Lavrijsen and were performed at the division of Drug Delivery Technology at the Leiden Academic Centre for Drug Research (LACDR), Leiden University, Leiden, The Netherlands. This research is supported by the Dutch Technology Foundation STW, which is part of the Netherlands Organisation for Scientific Research (NWO) and partly funded by the Ministry of Economic Affairs (project number 10064). In addition, the following companies provided substantial financial support to the research: Astellas, Evonik Industries, RiverD International B.V. and Unilever. The printing of this thesis was financially supported by STW. © 2013 Michelle Janssens. All rights reserved. No part of this thesis may be reproduced or transmitted in any form or by any means without written permission of the author. Cover image and formatting: Thijs van den Hoven. Printed by Proefschriftmaken.nl || Uitgeverij BOXPress
Atopic eczema:
The role of stratum corneum lipids in
the skin barrier
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden
op gezag van Rector Magnificus prof. mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties
te verdedigen op donderdag 30 mei 2013
klokke 13.45 uur
door
Michelle Janssens
geboren te Roosendaal en Nispen
in 1985
Promotiecommissie
Promotor: Prof. Dr. J.A. Bouwstra
Co-promotores: Dr. P.J. Caspers
Dr. A.P.M. Lavrijsen
Overige leden: Prof. Dr. M. Danhof
Prof. Dr. W. Jiskoot
Prof. Dr. E. Proksch
Dr. A. El Ghalbzouri
Voor mijn ouders
Aan Thijs
Table of contents
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List of abbreviations
AE atopic eczema
ANOVA analysis of variance
APCI atmospheric pressure chemical ionization
aSMASE acid sphingomyelinase
ATR attenuated total reflectance
a.u. arbitrary unit
AUC area under the curve
BCA bicinchoninic acid
BSA bovine serum albumin
CCD charge-coupled device
CER ceramide
CERS ceramide synthase
CHOL cholesterol
ED electron diffraction
ELA2 elastase 2
ELOVL elongation of very long chain fatty acids
FEG field emission gun
FFA free fatty acid
FLG filaggrin gene
FTIR Fourier transform infrared spectroscopy
FWHM full width half maximum
hex hexagonal
HPLC high performance liquid chromatography
IS internal standard
KLK kallikrein
LB lamellar body
LC-MS liquid chromatography-mass spectrometry
LEKTI lympho-epithelial Kazal-type-related inhibitor
LPP long periodicity phase
MCT mercury-cadmium-telluride
MUFA mono-unsaturated fatty acid
NeS Netherton syndrome
NMF natural moisturizing factor
orth orthorhombic
PAR2 proteinase-activated receptor 2
PCA 2-pyrrolidone-5-carboxylic acid
PPAR peroxisome proliferator-activated receptor
S/N signal to noise ratio
SAXD small angle X-ray diffraction
SB stratum basale
SC stratum corneum
SCD stearoyl-coenzyme A desaturase
SCORAD scoring atopic dermatitis
SD standard deviation
SEM standard error of the mean
SG stratum granulosum
SPINK5 serine protease inhibitor Kazal-type 5
SPP short periodicity phase
SS stratum spinosum
TEM transmission electron microscope
TEWL transepidermal water loss
TSLP thymic stromal lymphopoietin
UCA urocanic acid
Ceramide nomenclature
AdS α-hydroxy fatty acid amide-linked to a dihydrosphingosine base
AH α-hydroxy fatty acid amide-linked to a 6-hydroxysphingosine base
AP α-hydroxy fatty acid amide-linked to a phytosphingosine base
AS α-hydroxy fatty acid amide-linked to a sphingosine base
EOdS esterified ω-hydroxy fatty acid amide-linked to a dihydrosphingosine base
EOH esterified ω-hydroxy fatty acid amide-linked to a 6-hydroxysphingosine base
EOP esterified ω-hydroxy fatty acid amide-linked to a phytosphingosine base
EOS esterified ω-hydroxy fatty acid amide-linked to a sphingosine base
NdS non-hydroxy fatty acid amide-linked to a dihydrosphingosine base
NH non-hydroxy fatty acid amide-linked to a 6-hydroxysphingosine base
NP non-hydroxy fatty acid amide-linked to a phytosphingosine base
NS non-hydroxy fatty acid amide-linked to a sphingosine base
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