Identification of stripe rust resistance gene in candidate

Egyptian and CIMMYT wheat genotypes by Molecular

Markers Abu Aly , A.A.; A.A. Shahin; Doaa R. EL-Naggar and M. A. Ashmawy

Wheat Disease Research Department, Agricultural Research Center, Institute of Plant Pathology, ARC, Egypt

Email: a_aziz_egypt2007@yahoo.com, a.a.shahin@hotmail.com

Table1. Seedling response of monogenic lines and some

Egyptian and CIMMYT wheat genotypes to two Pst races and their adult plant reactions in the field over the 2012/13 and 2013/14 growing seasons.

Genotypes

Seedling infection

type (IT)

Disease severity at

adult stage

198E56 128E28 2012/13 2013/14

Yr1/6*Avocet S 0 0 0 0

Yr5/6*Avocet S 0 0 0 0

Yr6/6*Avocet S 8 7 60S 50S

Yr7/6*Avocet S 9 7 70S 70S

Yr8/6*Avocet S 8 7 60S 40S

Yr9/6*Avocet S 7 6 40S 40S

Yr10/6*Avocet S 2 0 0 0

Yr15/6*Avocet S 0 0 0 0

Yr17/6*Avocet S 2 1 10MR 5MR

Yr18/6*Avocet S 7 6 10S 10MS

Yr27/6*Avocet S 4 3 5MS 5MS

Yr32/6*Avocet S 2 2 0 0

YrSP/6*Avocet S 2 2 10MR 10MR

YrSK/6*Avocet S 8 7 10MR 5MR

Misr1 8 7 5MR 5MR

Misr2 7 7 10MR 5MR

Gemmeiza 9 7 8 5MS 10S

Gemmeiza 10 8 7 10MS 10MR

Gemmeiza 11 7 7 10MS 10MS

Sakha 93 7 8 30S 20MS

Sids-12 7 7 30S 10S

Sids-13 7 7 10MS 10MS

Giza 171 6 6 5MS 5MS

Line 6043 6 6 5R TrR

Line 6085 7 7 5MR 10MR

Line 6086 7 8 5R 10R

Line 6107 7 6 TrMR 5MR

Fig1. Marker Yr17 tested on diverse wheat genotypes and run on a agarose

gel. Lanes (1) monogenic Yr17; (2) Misr1; (3) Misr2 ;(4)Sakha93; (5)Sids12; (6)Sids13; (7)Gemmeiza9; (8)Gemmmeiza10; (9) Gemmeiza11; (10)Giza171; (11)Line6043; (12)Line6085; (13) Line6086; (14) Line6107. The arrow shows the fragment which is associated with Yr17.

Selected Reference: Robert O, Abelard C, Dedryver F (1999). Identification of

molecular markers for the detection of the yellow rust

resistance gene Yr17 in wheat. . In: Molecular Breeding, ,

5:167-175.

Introduction Bread wheat (Triticum aestivum L.) is the first important and

strategic cereal crop for the majority of world's populations and

Egypt as well. Stripe or yellow rust, caused by Puccinia

striiformis f. sp. tritici, is an important wheat disease and causes

considerable yield losses in wheat growing areas worldwide.

The objectives of the present study were to evaluate the

monogenic lines (Yr's) and candidate wheat genotypes under

greenhouse and field condition. In addition to identify of

resistance genes by three DNA markers.

Materials and Methods The present work was carried out at Sakha Agricultural

Research Station under greenhouse and field condition.

Fourteen monogenic lines and thirteen wheat genotypes

(nine Egyptian wheat cultivars and four entries from

CIMMYT-8STEMRRS) were evaluated at the seedling

stage under greenhouse conditions and the same germplasm

was also evaluated under field conditions against stripe rust.

In addition, Yr9, Yr17 and Yr18 resistance genes were

identified by molecular marker (Robert et al. 1999).

Results and Discussion Data showed that Yr1, Yr5, Yr10, Yr15, Yr17, Yr32 and

YrSP monogenic lines exhibited high resistance against

stripe rust reaction at seedling response, where the infection

type ranged from 0 to 2 with two races i.e. 198E56 and

128E28 (Table 1). Data also showed that at adult stage only

Yr1, Yr5, Yr10, Yr15, and Yr32 gave disease severity with

complete resistant reaction to stripe rust followed Yr17,

YrSP and YrSk, where their disease severity were 5MR-

10MR. Nine Egyptian cultivars and four lines from CIMYT

tested at seedling stage were susceptible (IT 6-8). Misr-1,

Misr-2, Gemmezia-10, Line 6043, Line 6085. Line 6086

and Line 6107 showed a ranged of adult plant responses

TrR to 10MR and the remainder had variable degree

susceptibility 5MS to 30S. Thirteen genotypes were

screened with three DNA markers aimed at detecting the

presence of Yr9, Yr17, and Yr18. The 1100bp diagnostic

for Yr9 was present in 7 genotypes i.e. Misr-1, Misr-2,

Sids-12, Sids-13, Gemmeiza-9, Gemmeiza-10 and

Gemmeiza-11 a 252bp fragment in Line6043 (8STEMRRS)

from CIMMYT was indicative of Yr17, (Fig. 1), and Yr18

was present in most Egyptian and CIMMYT genotypes

tested.

252bp

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