Assessing Vertical Microbial Transport in Bedrock Well Column › wp-content › uploads › 2017...
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Assessing Vertical Microbial Transport Assessing Vertical Microbial Transport Assessing Vertical Microbial Transport Assessing Vertical Microbial Transport
in Bedrock Well Columnin Bedrock Well Columnin Bedrock Well Columnin Bedrock Well Column
Eric J. Raes, P.E., LSRP
Engineering and Land Planning Engineering and Land Planning
Dora Ogles, Brett Baldwin, Kate Clark & Anita Biernacki
Microbial Insights Inc.
Kerry Sublette, Ph.D., Kate Key
University of Tulsa
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We We We We knowknowknowknow
That Hydrologist Like to
Draw Arrows
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We We We We knowknowknowknow
That Chemist Like using
Colors
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But what do we know about microbial But what do we know about microbial But what do we know about microbial But what do we know about microbial
migration?migration?migration?migration?
Let’s ask a friend.
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But what do we know about microbial But what do we know about microbial But what do we know about microbial But what do we know about microbial
migration?migration?migration?migration?
Let’s ask a friend.
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Microbial Release and Transport StudyMicrobial Release and Transport Study
0.100.120.140.160.180.20
met
er/d
ay
0.000.020.040.060.080.10
0.5 - 1 mm 0.25 - 0.5 mm 0.05 - 0.25 mm
met
er/d
ay
Sand Size Range
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Pilot Study Results - IW-1 and Piezometers
PZ-4
IW-1
PZ-3PZ-2 PZ-1
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Radius of Influence?Radius of Influence?
0.6016:30 05:42 18:54 08:06 21:18 10:30
-0 .100.080.260.440.620.80
DO
(mg
/L)
16:30 05:42 18:54 08:06 21:18 10:300.5100.5960.6820.7680.8540.940
Sp
Co
n(m
S/c
m)
16:30 05:42 18:54 08:06 21:18 10:3015.2015.3815.5615.7415.9216.10
Te
mp
(C)
WP-1.DAT
16:30 05:42 18:54 08:06 21:18 10:30-444.0-330.6-217.2-103.8
9.6123.0
OR
P(m
V)
16:30 05:42 18:54 08:06 21:18 10:305.806.046.286.526.767.00
pH
16:30 05:42 18:54 08:06 21:18 10:30
0.600.740.881.021.161.30 D
ep
th(m
)
DateTim e(M/D/Y )
12/07/11 12/14/11 12/20/11 12/27/11 01/02/12 01/09/12
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Star Pupils
••
GW Flow
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Anaerobic Bioreactors, the same but different
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100
1,000
10,000
100,000
8
10
12
14
16
(Cel
ls/m
L)
So
lven
t C
on
cen
trat
ion
s(u
g/L
)
MW-1 Solvent Concentrations vs. Time
0
1
10
0
2
4
6
(Cel
ls/m
L)
So
lven
t C
on
cen
trat
ion
s
Date
Tetrachloroethene Trichloroethene Vinyl Chloride cis, 1,2 DCE Dehalococcoides (DHC) tcaA Bav1
Legend
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Vertical Migration AssessmentVertical Migration Assessment
•• Chlorinated SolventsChlorinated Solvents
•• Deep water column with multiple fracturesDeep water column with multiple fractures
•• Non cooperative geoNon cooperative geo--chemical conditionschemical conditions
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MultiplMultiple e FracturFractureses
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ImplementationImplementation
TreatmentTreatment
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Chemical and GeoChemical and Geo--Chemical TrendsChemical Trends
3/18/2015 6/22/2015 9/17/2015
PCE 28.7 18.6 1.7
TCE 1230 676 7.5
cDCE 133 23.2 61.5cDCE 133 23.2 61.5
VC 0 0 1.9
Ethane 0 0 0
iron 316 103 8260826082608260
SO4 51.4 46.2 19.6
Methane 0 0.34 2040204020402040
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Microbial Ground Water ResultsMicrobial Ground Water Results
1.00E+03
1.00E+04
Microbial Populatons in GW
1.00E+00
1.00E+01
1.00E+02
3/1/2015 4/1/2015 5/1/2015 6/1/2015 7/1/2015 8/1/2015 9/1/2015
DHC BAV1 DHBt
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DHC vs DepthDHC vs Depth
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Chemical and GeoChemical and Geo--Chemical TrendsChemical Trends
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Chemical and GeoChemical and Geo--Chemical TrendsChemical Trends
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ImplementationImplementation
TreatmentTreatment
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Donor WarsDonor Wars
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Questions?Questions?