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Transcript of Ascorbic Acid Saturation Test & Determination of Ascorbic Acid Content of Some Fruit Juices. 376 BCH...
![Page 1: Ascorbic Acid Saturation Test & Determination of Ascorbic Acid Content of Some Fruit Juices. 376 BCH Miss Tahani Al-shehri.](https://reader036.fdocuments.net/reader036/viewer/2022082318/56649e535503460f94b49186/html5/thumbnails/1.jpg)
Ascorbic Acid Saturation Test & Determination of Ascorbic Acid Content of Some Fruit Juices.
376 BCHMiss Tahani Al-shehri
![Page 2: Ascorbic Acid Saturation Test & Determination of Ascorbic Acid Content of Some Fruit Juices. 376 BCH Miss Tahani Al-shehri.](https://reader036.fdocuments.net/reader036/viewer/2022082318/56649e535503460f94b49186/html5/thumbnails/2.jpg)
OBJECTIVES
•To measure the concentration of ascorbic acid urine sample after administration of a dose of ascorbic acid.
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Introduction
•Vitamin C is a water-soluble vitamin that is necessary for normal growth and development.
•Water-soluble vitamins dissolve in water. The body cannot store them. Leftover amounts of the vitamin leave the body through the urine. That means you need a continuous supply of such vitamins in your diet.
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Introduction
Ascorbic acid Dehydroscorbic acid
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Functions of Vitamin C
•Vitamin C is required for the growth and repair of tissues in all parts of your body.
•It is necessary to form collagen•Vitamin C is one of many antioxidants in
the body * The body does not manufacture vitamin C
on its own ( Why ? )
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Deficiency of Ascorbic acid
•“ Scurvy “
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ascorbic acid saturation test
•Administered ascorbic acid is taken up by the tissues until they are saturated, after which there is a sharp rise in the urinary ascorbic acid excretion.
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Principle
•In a protein-free filtrate of plasma or urine, ascorbic acid has the property of decolorizing a blue dye, dichloro-phenolindophenol DCPIP and the amount that is decolorized is a measure of the amount of the vitamin C present.
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Principle
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Notes
• Estimation of urinary ascorbic acid when the specimen arrives in the laboratory must carried out
immediately .
• Ascorbic acid is unstable in urine and a special bottle containing 20 ml of glacial acetic acid must be used for the urine collection.
• Glacial acetic acid is corrosive and any spilt on the skin should immediately be washed off with a large volume of water.
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METHOD
1. Measure the volume of the urine. 2. Pipette 5 ml of urine into a 100 ml
volumetric flask and make up to the mark with 5% acetic acid.
3. Pipette 5 ml of DCPIP dye solution into a conical flask.
4. Titrate with standard ascorbic acid solution (0.02 mg/ ml) until the solution just becomes colourless.
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METHOD cont..
5. Calculate how many milligrams of ascorbic acid are equivalent to 5 ml of the dye solution.
6. Pipette 5 ml of dye into a conical flask. 7. Titrate with the dilute urine sample. The
titration must be done very rapidly, otherwise slow-reacting reducing substances present in the urine will contribute to the reduction of the dye
8. Repeat the titrate and take the average
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METHOD cont..
9. Calculate how much ascorbic acid present in sample urine diluted
10.Calculate milligrams of ascorbic acid in total urine collected .
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Results •Volume of urine =…………………………….•Mg of ascorbic acid equivalent to 5 ml of the
dye solution =…………………………………………
•The conc. Of ascorbic acid present in sample urine diluted =……………………………………
•Mg of ascorbic acid in total urine collected =…………………………………………………………