Supplementary material

Interindividual Variability of Soil Arsenic Metabolism by Human Gut

Microbiota Using SHIME Model

Naiyi Yin a, b, Huili Du a, b, Pengfei Wang a, b, Xiaolin Cai a, b, Peng Chen b, Guoxin Sun b,

Yanshan Cui a, b, *

a College of Resources and Environment, University of Chinese Academy of Sciences, Beijing

101408, People’s Republic of China

b Research Center for Eco-Environment Sciences, Chinese Academy of Sciences, Beijing

100085, People’s Republic of China

*Corresponding Author

Tel: +86-10-6967 2968

E-mail: cuiyanshan@ucas.ac.cn

2 Tables; 3 Figures.

Table S1

Correlation matrix between concentrations of Fe, Mn, and As in colon digests (n = 95).

Fe Mn As

Fe 1.000 -0.430** 0.740**

Mn 1.000 -0.527**

As 1.000

**Correlation is significant at the 0.01 level

Table S2

Relative abundance of gut bacterial genera in this study according to previous reports.

Genus Relative abundance Taxa References

Adult Child

Aeromonas 0 0.002 k__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Aeromonadales;f__Aeromonadaceae;g__Aeromonas; Bentley and Chasteen (2002)

Alcaligenes 0.082 0.713 k__Bacteria;p__Proteobacteria;c__Betaproteobacteria;o__Burkholderiales;f__Alcaligenaceae;g__Alcaligenes; Bentley and Chasteen (2002)

Alistipes 2.416 0.414 k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Rikenellaceae;g__Alistipes; Yu et al. (2016)

Alkaliphilus 0.011 0.004 k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae_2;g__Alkaliphilus; Fisher et al. (2008)

Bacteroides 35.868 41.447 k__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Bacteroidaceae;g__Bacteroides; Newman et al. (1997)

Bifidobacterium 0.087 0.241 k__Bacteria;p__Actinobacteria;c__unidentified_Actinobacteria;o__Bifidobacteriales;f__Bifidobacteriaceae;g__Bifidobacterium; Lee et al. (2008)

Bilophila 0.624 0.065 k__Bacteria;p__Proteobacteria;c__Deltaproteobacteria;o__Desulfovibrionales;f__Desulfovibrionaceae;g__Bilophila; Yu et al. (2016)

Cloacibacillus 4.995 0.189 k__Bacteria;p__Synergistetes;c__Synergistia;o__Synergistales;f__Synergistaceae;g__Cloacibacillus; Yu et al. (2016)

Clostridium 0 0.085 k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae_1;g__Clostridium; Stolz (2006)

Desulfovibrio 0.722 13.222 k__Bacteria;p__Proteobacteria;c__Deltaproteobacteria;o__Desulfovibrionales;f__Desulfovibrionaceae;g__Desulfovibrio; Kocar et al. (2010)

Escherichia-Shigella 18.470 2.930 k__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacteriales;f__Enterobacteriaceae;g__Escherichia-Shigella; Saltikov and Olson (2002)

Exiguobacterium 0.225 0.004 k__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Family_XII;g__Exiguobacterium; Cai et al. (2016)

Klebsiella 3.443 4.589 k__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacteriales;f__Enterobacteriaceae;g__Klebsiella; Maeda et al. (1992)

Lactobacillus 0.022 0.011 k__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus; Lee et al. (2008)

Methanosarcina 0.015 0 k__Archaea;p__Euryarchaeota;c__Methanomicrobia;o__Methanosarcinales;f__Methanosarcinaceae;g__Methanosarcina; Wang et al. (2014)

Proteus 0.145 0.186 k__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacteriales;f__Enterobacteriaceae;g__Proteus; Shariatpanahi et al.( 1981)

Pseudomonas 0.052 0.215 k__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas; Chen et al. (2013)

Shewanella 0.041 0.004 k__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Alteromonadales;f__Shewanellaceae;g__Shewanella; Lee et al. (2007)

Sulfurospirillum 0.013 0.061 k__Bacteria;p__Proteobacteria;c__Epsilonproteobacteria;o__Campylobacterales;f__Campylobacteraceae;g__Sulfurospirillum; Zobrist et al. (2000)

Veillonella 0 0.009 k__Bacteria;p__Firmicutes;c__Negativicutes;o__Selenomonadales;f__Veillonellaceae;g__Veillonella; Bentley and Chasteen (2002)

Fig. S1. As bioaccessibility (mean ± SD) in small intestinal (Intestine) and colon (C12, C24,

and C48: colon 12, 24, and 48 h) phases of eight As-contaminated soils (n = 3). Note the

different scales for the As bioaccessibility (y-axis). Abbreviations: PA, PBET-SHIME (Adult);

PC, PBET-SHIME (Child); UA, UBM-SHIME (Adult); UC, UBM-SHIME (Child).

Fig. S2. Concentration (mean ± SD) of chromatographically detected As species in colon

digests of eight As-contaminated soils (n = 3). Note the different scales for the As

concentrations (y-axis). Abbreviations: PA, PBET-SHIME (Adult); PC, PBET-SHIME

(Child); UA, UBM-SHIME (Adult); UC, UBM-SHIME (Child).

Fig. S3. The gut microbiota composition profiles at the phylum level in the adult and child.

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