TaqMan®

Campylobacter jejuni Detection Kit

Fast, easy, accurate detection

TaqMan® Pathogen Detection Kits

• Campylobacter jejuni

• Cronobacter sakazakii

• E. coli O157:H7

• Listeria monocytogenes

• Pseudomonas aeruginosa

• Salmonella enterica

• Staphylococcus aureus

Applied Biosystems Food Pathogen Detection System

A Growing DemandCampylobacter jejuni and Campylobacter coli cause more cases of bacteria diarrheal illness than Shigella and Salmonella combined. However, strains are difficult to cultivate and low levels may go undetected using selective enrichment. This has created a demand for a rapid C. jejuni test method that does not require isolation and culture.

Real-Time PCR DeliversThe TaqMan Campylobacter jejuni Detection Kit uses Real-Time PCR technology designed to provide:

• Faster time to results

• Excellent specificity and sensitivity

• Exceptional ease of use

• Reduced risk of contamination

Faster Time to ResultTraditional Campylobacter jejuni detection methods can take up to 6 days; immunoassay methods can take 2 days or more. After a 24 hour pre-enrichment step, time to result with the TaqMan Campylobacter jejuni Detection Kit is less than 4 hours and less than 28 hours total.

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Results in three easy steps

1. Enrich samples

2. Prepare samples

3. Run samples

Demonstrated Specificity and SensitivityThe TaqMan Campylobacter jejuni Detection Kit has demonstrated:

• 100% inclusivity for 27 strains of Campylobacter jejuni and Campylobacter coli

• 100% exclusivity for 29 other non-Campylobacter and non-jejuni or coli strains

• Detects down to 1 cfu in 25 grams or enriched food sample

Exceptional Ease of Use• Three easy steps

• Software-guided assay procedure

• No electrophoresis or post-PCR processing

The TaqMan Campylobacter jejuni Detection Kit gives you reliable results in just 3 simple steps. RapidFinder™ software guides you through the assay procedure, and displays results on an easy-to-read screen for fast, accurate interpretation.

Minimal Risk of ContaminationThe TaqMan assay uses a closed-tube format. Once pipetted, tubes remain closed throughout the amplification and detection process, minimizing risk of cross contamination.

Preformatted and Ready to UseThe TaqMan Campylobacter jejuni Detection Kit includes reagents for preparation and amplification of 100 samples, including:

• Campylobacter jejuni target assay mix

• Environmental master mix

• Negative control

Each reaction contains an Internal Positive Control (IPC) that controls for the presence of inhibitors for reliable negative results.

Part of a Growing Family Applied Biosystems offers a full line of TaqMan Pathogen Detection Kits:

• Campylobacter jejuni 1 (p/n 4366098)

• Salmonella enterica 1 Certified by AOAC and AFNOR (p/n 4366104)

• Cronobacter sakazakii 2 (p/n 4382492)

• E. coli O157:H7 1 (p/n 4366100)

• Listeria monocytogenes 1 (p/n 4366102)

• Pseudomonas aeruginosa 2 (p/n 4368604)

• Staphylococcus aureus 2 (p/n 4368606)

1 These kits have been validated as described in TaqMan® Pathogen Detection Kits–Validation Reference White Paper (stock number 116WP01-01).

2 These kits have been verified through Applied Biosystems’ internal verification procedures.

These kits are based on universal assay conditions, enabling you to run any combination of these assays together in the same sample run.

A Complete SolutionIn combination with Applied Biosystems Real-Time PCR Systems such as the 7300, 7500, 7500Fast, and StepOne™ Systems, RapidFinder software, and the PrepMan® Ultra Sample Preparation Reagent, the TaqMan Campylobacter jejuni Detection Kit offers a fully-integrated, single-vendor solution that provides superior performance, reliability, and ease-of-use.

For environmental (including soil, air and water), quality control, and food and finished products testing, and for research use only. Not for use in diagnostic procedures. This product has not been cleared or otherwise approved by the United States Food and Drug Administration or by any other regulatory body in any country, or under the European IVD Directive, for diagnostic or any other clinical purpose. The purchaser or user of this product agrees not to use this product for diagnostic or other clinical purposes.

NOTICE TO PURCHASER: LIMITED LICENSE. Use of the TaqMan® Campylobacter jejuni Pathogen Detection Kit (p/n 4366098) is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 6,127,155, 5,677,152 (claims 1-23 only), and 5,773,258 (claims 1 and 6 only), 5,210,015, 5,487,972, 5,804,375, 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product solely in environmental testing, quality control/quality assurance testing and food testing applications including reporting results of purchaser’s activities for a fee or other commercial consideration, and also for the purchaser’s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) is conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

© Copyright 2008, Applied Biosystems. All Rights Reserved. AB (Design), Applera, Applied Biosystems and PrepMan are registered trademarks and RapidFinder and StepOne are trademarks of Applera Corporation or its subsidiaries in the U.S. and/or certain other countries. TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Printed in the USA, 12/2008 Publication 116MI09-03