Appendix

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Appendix figure1. Disection of Cattleya flower. Source : http://www.nihongo.com/matsuura/Cattleya/Kakubu.htm.

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Appendix Figure 2. pDrive Cloning Vector Source : http://www1.qiagen.com/literature/images/pDrive_cloning_vector

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53 species of Cattleya from Mexico to South America - Cattleya aclandiae : Lady Ackland's Cattleya (Brazil) - Cattleya amethystoglossa : Amethyst-lipped Cattleya (Brazil) - Cattleya araguaiensis : Araguaia Cattleya (Brazil) - Cattleya aurantiaca : Orange Cattleya (Mexico to C. America). - Cattleya bicolor : Bicolored Cattleya (SE. Brazil)

- Cattleya bicolor subsp. bicolor (Brazil). Pseudobulb epiphyte - Cattleya bicolor subsp. canastrensis (Brazil) . Pseudobulb epiphyte - Cattleya bicolor subsp. minasgairensis (Brazil). Pseudobulb epiphyte

- Cattleya boissieri Colombia. - Cattleya bowringiana : Bowring's Cattleya (Mexico to Honduras). - Cattleya × brasiliensis (= C. bicolor × C. harrisoniana) (Brazil) . - Cattleya × brymeriana (= C. violacea × C. wallisii) (N. Brazil). - Cattleya candida (Colombia). - Cattleya × colnagiana (Brazil). - Cattleya × dayana (= C. forbesii × C. guttata) (Brazil). - Cattleya × dolosa (= C. loddigesii × C. walkeriana): Dolose Cattleya, Crafty Cattleya, Deceitful Cattleya (Brazil). - Cattleya dormaniana : Dorman's Cattleya (Brazil) - Cattleya dowiana : Queen of the Cattleyas, Dow's Cattleya (Costa Rica to Colombia).

- Cattleya dowiana var. aurea : Golden-yellow Cattleya (S. Panama to Colombia). Pseudobulb epiphyte - Cattleya dowiana var. dowiana (Costa Rica). Pseudobulb epiphyte

- Cattleya × dukeana (C. bicolor × C. guttata) (SE. Brazil). - Cattleya dupontii (Brazil). - Cattleya × duveenii ( = C. guttata × C. harrisoniana) (SE. Brazil). - Cattleya elegantissima (Venezuela). - Cattleya elongata : Cattleya with the Elongated Stalk (Brazil) - Cattleya forbesii : Forbes' Cattleya (Brazil)

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- Cattleya gaskelliana : Gaskell's Cattleya (Colombia to Trinidad). - Cattleya × gransabanensis (= C. jenmanii × C. lawrenceana) (Venezuela). - Cattleya granulosa : Granulose Cattleya (Brazil) - Cattleya × guatemalensis (= C. aurantiaca × C. skinneri.) : Guatemalan Cattleya (SE. Mexico to C. America). National flower of Guatemala - Cattleya guttata : Spotted Cattleya (Brazil). - Cattleya × hardyana ( = C. dowiana var.aurea × C. warscewiczii): Hardy's Cattleya (Colombia). - Cattleya harrisoniana : Harrison's Cattleya (SE. Brazil). - Cattleya herbacea (NE. Argentina). - Cattleya × hybrida (= C. guttata × C. loddigesii) (SE. Brazil). - Cattleya × imperator ( = C. granulata × C. labiata) (NE. Brazil). - Cattleya intermedia : Intermediate Cattleya (SE. & S. Brazil, Paraguay, Uruguay). - Cattleya × intricata (=. C. intermedia × C. leopoldii) (S. Brazil). - Cattleya iricolor : Rainbow-colored Cattleya (Ecuador to Peru). - Cattleya × isabella (.= C. forbesii × C. intermedia) (SE. Brazil). - Cattleya × itatiayae (SE. Brazil). - Cattleya jenmanii : Jenman's Cattleya (Venezuela to Guyana). - Cattleya × joaquiniana ( = C. bicolor × C. walkeriana) (Brazil) . - Cattleya × kautskyi (= C. harrisoniana × C.) (SE. Brazil). - Cattleya kerrii : Kerr's Cattleya (Brazil). - Cattleya labiata : Crimson Cattleya, Ruby-lipped Cattleya (Brazil) - Cattleya lawrenceana : Sir Trevor Lawrence's Cattleya (Venezuela, Guyana, N. Brazil). - Cattleya loddigesii : Loddiges' Cattleya (SE. Brazil to NE. Argentina).

- Cattleya loddigesii subsp. loddigesii (SE. Brazil to NE. Argentina). Pseudobulb epiphyte

- Cattleya loddigesii subsp. purpurea (Brazil). Pseudobulb epiphyte - Cattleya × lucieniana ( = C. forbesii × C. granulosa) (SE. Brazil). - Cattleya lueddemanniana : Lueddemann's Cattleya (N. Venezuela).

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- Cattleya luteola : Pale-yellow Cattleya (N. Brazil, Ecuador to Bolivia). - Cattleya maxima : Greatest Cattleya, Christmas Flower (Venezuela to Peru). - Cattleya × measuresii ( = C. aclandiae × C. walkeriana) (E. Brazil). - Cattleya mendelii : Mendel's Cattleya (NE. Colombia). - Cattleya × mesquitae ( = C. nobilior × C. walkeriana) (Brazil). - Cattleya × mixta ( = C. guttata × C. schofieldiana) (Brazil). - Cattleya × moduloi (C. schofieldiana × C. warneri) (Brazil). - Cattleya mooreana : Moore's Cattleya (Peru). - Cattleya mossiae : Easter Orchid, Mrs. Moss' Cattleya (N. Venezuela) - Cattleya motae (Brazil). - Cattleya nobilior : Noble Cattleya (WC. Brazil to Bolivia). - Cattleya patinii : Patin's Cattleya (Costa Rica to Venezuela, Trinidad). - Cattleya × patrocinii (= C. guttata × C. warneriana): Patrocinio's Cattleya (SE. Brazil). - Cattleya percivaliana : Christmas orchid, Percival's Cattleya (Colombia to W. Venezuela). - Cattleya × picturata ( = C. guttata × C. intermedia) (SE. Brazil). - Cattleya porphyroglossa : Purple-lipped Cattleya (Brazil). - Cattleya × resplendens ( = C. granulosa × C. schilleriana) (NE. Brazil) - Cattleya rex : King of the Cattleyas (Colombia to N. Peru). - Cattleya schilleriana : Consul Schiller's Cattleya (Brazil). - Cattleya schofieldiana : Schofield's Cattleya (Brazil) - Cattleya schroderae : Easter Orchid, Baroness Schroder's Cattleya (NE. Colombia). - Cattleya × scita (= C. intermedia × C. tigrina) (S. Brazil). - Cattleya skinneri : Flower of San Sebastian, Skinner's Cattleya (SE. Mexico to C. America). - Cattleya storeyi (Windward Is.) (Barbados.) - Cattleya × tenuata (= C. elongata × C. tenuis) (Brazil) . - Cattleya tenuis : Slender-stemmed Cattleya (NE. Brazil). - Cattleya tigrina (SE. & S. Brazil).

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- Cattleya trianae : Dr. Triana's Cattleya (Colombia). - Cattleya × undulata ( = C. elongata × C. schilleriana) (Brazil). - Cattleya velutina : Velvety Cattleya (Brazil) - Cattleya × venosa (= C. forbesii × C. harrisoniana) (Brazil). - Cattleya × victoria-regina ( C. guttata × C. labiata) (NE. Brazil). - Cattleya violacea : Superba of the Orinoco, Violet Cattleya (S. Trop. America). - Cattleya walkeriana : Walker's Cattleya (WC. & SE. Brazil). - Cattleya wallisii (N. Brazil). - Cattleya warneri : Warner's Cattleya (E. Brazil). - Cattleya warscewiczii : Warscewicz's Cattleya (Colombia). - Cattleya whitei (Brazil) - Cattleya × wilsoniana ( = C. bicolor × C. intermedia). (Brazil).

Source : http://en.wikipedia.org/wiki/Cattleya

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Appendix Table 1. Abbreviations of amino acids

Amino acid Three-letter abbreviation One-letter symbol Alanine Ala A Arginine Arg R

Asparagine Asn N Aspartic acid Asp D

Cysteine Cys C Glutamine Gln Q

Glutamic acid Glu E Glycine Gly G Histidine His H

Isoleucine Ile I Leucine Leu L Lysine Lys K

Methionine Met M Phenylalanine Phe F

Proline Pro P Serine Ser S

Threonine Thr T Tryptophan Trp W

Tyrosine Tyr Y Valine Val V

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Appendix Table 2. The genetic code

First position (5’end)

Second position

Third position (3’end)

U C A G Phe Ser Tyr Cys U Phe Ser Tyr Cys C Leu Ser Stop Stop A

U

Leu Ser Stop Trp G Leu Pro His Arg U Leu Pro His Arg C Leu Pro Gln Arg A

C

Leu Pro Gln Arg G Ile Thr Asn Ser U Ile Thr Asn Ser C Ile Thr Lys Arg A

A

Met Thr Lys Arg G Val Ala Asp Gly U Val Ala Asp Gly C Val Ala Glu Gly A

G

Val Ala Glu Gly G

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