A&P 1 - Lab #2 Microscopy Cell Structure Mitosis.

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A&P 1 - Lab #2 Microscopy Cell Structure Mitosis

Transcript of A&P 1 - Lab #2 Microscopy Cell Structure Mitosis.

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A&P 1 - Lab #2

Microscopy

Cell Structure

Mitosis

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Rotatable Head

Ocular Lens

Objective Lens

Arm

Illuminator

Condenser

Stage

Base

Course Adjustment Knob

Fine Adjustment Knob

Mechanical Stage

Mechanical Stage Control

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Magnification

• Total magnification is achieved by multiplying the power of the ocular lens by the power of the objective lens

Total Magnification = Ocular Lens X Objective Lens

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Objective Lens

• Red Band = Low Power = 4

4(obj lens) X 10(ocular lens) = 40X

• Yellow Band = Medium Power = 10Yellow Band = Medium Power = 10

10(obj lens) X 10(ocular lens) = 100X10(obj lens) X 10(ocular lens) = 100X

• Blue Band = High Power = 100Blue Band = High Power = 100

40(obj lens) X 10(ocular lens) = 400X40(obj lens) X 10(ocular lens) = 400X

• White Band = Oil Immersion

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Follow the Rules!

• ALWAYS begin on LOW POWER– Once you have the specimen in focus– Move to medium power– Refocus, then you can move to high power

• Once you move off low power, you can NEVER, NEVER, NEVER use the coarse adjustment

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Orientation of Specimen

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Specimen is flipped horizontally & vertically

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Depth of Field

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Preparing a Wet Mount

Cover Slip 45O

Slowly LowerCover Slip

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Air Bubbles - Try Again!

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Types of Cells

• There are 2 types of cells:

Prokaryotic Eukaryotic (pro- before) (eu- true)

-kary = nucleus

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Prokaryotic Cells

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Prokaryotic Cells

Prokaryotic cells -lack a membrane-bound nucleus.-genetic material is present in the nucleoid

ALL prokaryotic cells are bacteria, and ALL bacteria are prokaryotic

The only place you will find prokaryotic cells in is the KINGDOM BACTERIA AND ARCHEBACTERIA

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Eukaryotic Cells

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Epithelial - Cheek Cells

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The Cell Cycle

• Interphase

• Mitosis– Prophase - prepare– Metaphase - middle– Anaphase - apart– Telophase - two

• Cytokinesis cyto- = cell -kinesis = to move

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The Cell Cycle

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Interphase

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Mitosis - Prophase

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Mitosis - Metaphase

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Mitosis - Metaphase

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Mitosis - Anaphase

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Mitosis - Anaphase

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Mitosis - Telophase

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Mitosis - Telophase/Cytokinesis

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Practice Identifying the Stages of Mitosis

• Mitosis

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