A.O.T.F Laser control
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Transcript of A.O.T.F Laser control
A.O.T.F Laser control
• Thermally controlled Tellerium Dioxide (TeO2) crystal
Selected Wavelength
Kr/Ar Laser Light
Transducer
Non DefractedWavelengths
Acoustic Termination
BeamDump
Acousto-Optic-Tunable-Filter Principle
Specifications
• Polarisation preserving >70:1 extinction
• > 85% transmision efficiency
• blocking > OD 4.0
• Noise = < 5.0% peak RMS (measured in reflection imaging mode as per std. commissioning spec.)
• switching frequency - how quick can you click a mouse button!
AOTF DELIVERS High quality simultaneous multi label imaging
•A comparison of classic Neutral Density (ND) attenuation versus AOTF laser control
Spectral separation considerations
AOTF allows more control of the laser intensities to allowbetter assessment of dye co-localisation
FITC channel (522/32) using ND filter
CY3 channel (HQ598/40) using ND filter
CY5 channel (680/32) using ND filter
The FITC channel is bleeding into the CY3 channel in this set-up
24 Bit merge of FITC/CY3/CY5 using ND filter
Cross talk between red and green channels
Fluorogram of FITC & CY3 channels showing FITC bleed through (cross talk)
FITC channel (522/32) using AOTF control
CY3 channel (HQ598/40) using AOTF control
CY5 channel (680/32) using AOTF control
24 Bit merge of FITC/CY3/CY5 using AOTF control
Fluorogram of FITC & CY3 channels with AOTF control - displaying little co-localisation
The Comparison Again
AOTF imaging ND filter imaging
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