• Introduction • Electrode/electrolyte interface • Biopotential electrodes
“Integrated CNS and CV monitoring via telemetry in behavior … · 2019-04-11 · PhysioTel...
Transcript of “Integrated CNS and CV monitoring via telemetry in behavior … · 2019-04-11 · PhysioTel...
© 2012 Data Sciences International
The Gold Standard in Physiologic Monitoring
“Integrated CNS and CV monitoring via
telemetry in behavior studies”
Thomas Penning
European Manager at Data Sciences International
Measurement Behavior 2012
- Tutorial -
© 2012 Data Sciences International
Agenda
DSI Company Overview
New telemetry products for large & small animals
Key features for accurate & reliable chronic monitoring
New possibilities by the use of customized antennas
Examples for combined CNS/CV + behavior monitoring
Discussion, Q&A Session
© 2012 Data Sciences International
Questions?
© 2012 Data Sciences International
DSI Business Field
DSI was founded in 1984 and
growed to the global leader in
implantable telemetric
physiological monitors
Founded in 1986 as spinout of
Buxco Company focused in
physiological signal analysis
More than 70 years experience in
medical instrumentation and high
reputation for signal conditioning
& recording
Provider of telemetric & hardwired solutions
for cardiovascular, CNS and respiratory
monitoring & analysis in preclinical research
© 2012 Data Sciences International
28 Years of history (founded 1984)
180+ Employees
Systems in 48 Countries
18 Sales offices
500,000+ Subjects monitored
1000+ Publications
30+ Patents
20% Revenue spending in R&D
Data Sciences in Figures
Brian Brockway
DSI founder
© 2012 Data Sciences International
Implantable Telemetry System
© 2012 Data Sciences International
DSI Wireless Monitoring Parameters
Core Body Temperature
Blood Pressure
Respiration (breath frequency)
ECG – electrocardiogram
EMG - electromyogram
EOG – electro-oculogram
EEG – electroencephalogram
LVP, PAP,
Intra-Pleural Pressure
SNA
Bladder Pressure
Intracavernosal Pressure
Many More…
© 2012 Data Sciences International
Implantable Family
Wide application
Reduced stress
Minimized variables
Increased data accuracy
Pressure Temperature
Biopotential
© 2012 Data Sciences International
New Telemetry Offerings
Physiotel HD Series
Physiotel Digital
Receiver RSC-1 / RSC-3
DSI Business Services
© 2012 Data Sciences International
What is ‘HD’?
HD = Hybrid Digital telemetry…
New transmitter for small animals
Improved reliability & performance
More signals & parameter combinations
Provide improved reliability & performance.
Improved animal welfare
Works with existing hardware*
* Software update required
© 2012 Data Sciences International
PhysioTel HD Platform Overview
Digital encoded data
Device ID & serial number
Calibration values
Battery status and battery “ON” time
High performance pressure sensing
No wrong data due crosstalk
Automated Xmitter configuration
© 2012 Data Sciences International
HD-S21 Dual-pressure Small Animal Telemetry
Signals Measured
Two pressures
Biopotential
Temperature
Activity
Intended Species
Rat
Ferret
Guinea pig
Marmoset
Battery: 2 month
Volume: 5.9cc
Weight: 8g
Implantable at an animal body weight of 175g
© 2012 Data Sciences International
Application opportunities with HD-S21
LV
P
B
P
EC
G
Chronic monitoring applications:
– Left ventricular pressure + blood pressure (BP)
– Pulmonary artery pressure + BP
– Pleural pressure + BP
– Pulse wave velocity
– Intra-ocular pressures
– Uterus pressure + BP
– Bladder pressure + BP
Precise, reliable, high fidelity data is
available with an HD-S21.
© 2012 Data Sciences International
LVP, BP & ECG with HD-S21
© 2012 Data Sciences International
HD-S21 Chronic Parameter Data
© 2012 Data Sciences International
HD-X11 Transmitter
Signals Measured
Pressure
ECG, EMG or EEG
Temperature
Activity
Intended Species
Mouse
Hamster
Juvenile rat
Plus more!
Volume: 1.4cc
Weight: 2.2g
Battery Life: 30 days
IP implantable from a min. body weight of 30g
SC implantable from a min. body weight of 19g
© 2012 Data Sciences International
New Capabilities with the HD-X11
Consolidated study of multiple
parameters:
Reduce in animal use
Higher statistical relevance
More complete physiologic
assessment in CV studies
HD-X11 components
held together with
flexible circuit
© 2012 Data Sciences International
Simultaneous BP & ECG from a Single Mouse!
© 2012 Data Sciences International
HD-X11 parameter data
© 2012 Data Sciences International
PhysioTel Digital Implants
Model L21 2 Pressures
1 Biopotential
Temperature
Activity
Battery Life: 12 weeks
Model L11 1 Pressure
1 Biopotential
Temperature
Activity
Battery Life: 15 weeks
Technical
Data
© 2012 Data Sciences International
Spec Comparison
D70 - PCT L11
Weight 47grams 56 grams
Volume 32cc 29cc
Pressure Drift < 2 mmHg/month max. < 2 mmHg/month max.
Battery Life 14 weeks 15 weeks
RF - Range 1m 3-5m
Bandwidth Biopotential 1 – 100Hz 0.1 – 100Hz
Biopotential Range Fixed Programmable
© 2012 Data Sciences International
Implantation in Primates
BP catheter with
radio opaque marker
Intravascular (negative) ECG
Leadin superior vena cava
Positive ECG lead
loop
Antenna inserted in an
intramuscular pocket
© 2012 Data Sciences International
System Equipment
In the animal
In the cage
In the room
DSI Telemetry
D70
RMC-1
DEM
PhysioTel
L21, L11
TRX-1
CLC
PhysioTel
Digital
© 2012 Data Sciences International
Key specs of the system
Digital data
No cross talk
Less disturbances
Auto-configuration
Longer range (3-5m)
Less receivers
Multi animals/receiver
Group housing (up to 12)
© 2012 Data Sciences International
Example Canine System
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Accurate Biopotential Sensing
Biopotential
Expanded bandwidth 0.1 – 100 Hz
Solid Tip electrode for clean ECGs
© 2012 Data Sciences International
Primate Data
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Dog Data
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Minipig Data
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Example Primate Set-up
© 2012 Data Sciences International
Surgery Support for DSI Products
Training Workshops
Quarterly courses at DSI
Harlan Courses in EU
Labs at AALAS,CALAS, ASR
Surgical Support Services:
Surgical Videos and manuals
Experienced surgeons at DSI
Customer reference sites
Trained implant services vendors
Taconic
Charles River Laboratories
The Jackson Laboratory
DSI Support
Trained Technical Sales Representatives,
Tech Support Staff
© 2012 Data Sciences International
Keys for accurate chronic monitoring
Pressure Sensing Techniques
Battery challenges
Sampling frequency and bandwidth
Housing and environment
© 2012 Data Sciences International
Sensing Technology Considerations
© 2012 Data Sciences International
Summary – Sensing Technology
Good adoption of frequency to phys. signals
High temperature stability (less drift)
Proven reliability in chronic applications
Easy to remove without damage
Inexpensive replacement (refurbishment)
Antithrombotic & proven biocompatibility
© 2012 Data Sciences International
Maintenance cost
Self maintenance with re-gel procedure
Transmitter exchange program
Durablity Cost
solid tip fluid filled
0 €
500 €
1,000 €
1,500 €
2,000 €
2,500 €
3,000 €
HD-X11 new Refurbishment
© 2012 Data Sciences International
Exchange Program
Factory site renewal of:
Catheter
Lead electrodes
Housing
Battery
Calibration
Sterilization
Sterile Package
Last approx. 3 weeks
Returned transmitter “As New”
Warranty of up to 18 month*
* MWP = Maximum Warranty Period (Type depended)
© 2012 Data Sciences International
Signal Frequency Characteristic
Drop in amplitude
Slow rise time
Inaccurate contractility
Increase of noise
More disturbances
Same physiol. info
higher
Bandwidth
smaller
Bandwidth Hz kHz
The frequency characteristic of a catheter is the ability to follow
pressure changes over time. The higher the HR the higher needs to
be the bandwidth of the catheter.
© 2012 Data Sciences International
LVP signal in the frequency domain
HR = 320bpm = 5.3 Hz This amplitude spectrum shows which
catheter frequency would be necessary
for a accurate reproduction of a
ventricular pressure signal.
Higher frequency portions then the heart
frequency are seen due the non-
sinusoidal shape of the LVP signal
© 2012 Data Sciences International
Catheter Frequency Characteristics
The frequency characteristic of
a catheter depends on material
and length.
The shorter the catheter the
higher the bandwidth
Empirically the bandwidth for
BP monitoring should be
5 times higher than the heart
frequency.
© 2012 Data Sciences International
Rat LVP – two catheter types Time rel. Time abs. Date Num Sys LVEDP DP HR dP/dt Time rel. Time abs. Date Num Sys LVEDP DP HR dP/dt
0000:29:53 11:18:15 1/27/2009 397 151 21 131 320 9394 0000:29:53 11:18:15 1/27/2009 397 151 21 131 320 9111
0000:29:53 11:18:15 1/27/2009 398 151 20 131 320 9607 0000:29:53 11:18:15 1/27/2009 398 151 20 131 320 9138
0000:29:53 11:18:15 1/27/2009 399 150 19 131 321 9603 0000:29:53 11:18:15 1/27/2009 399 150 20 130 320 9192
0000:29:54 11:18:16 1/27/2009 400 149 19 129 318 9394 0000:29:54 11:18:16 1/27/2009 400 149 19 129 319 9151
100Hz
2 kHz
© 2012 Data Sciences International
Catheter Bandwidth for BP Monitoring
higher
Bandwidth
smaller
Bandwidth
Drop in amplitude
Slow rise time
Inaccurate contractility
Increase of noise
More disturbances
Same physiol. info
Hz kHz
Depends on the HR of the animal
BP Rat: 300bpm (5Hz) * 5 = 25Hz
Mouse 900bpm (15Hz) * 5 = 75Hz
© 2012 Data Sciences International
Drift Compensation
Bridge in DSI transmitter housing Bridge in solid tipped catheters
Cath. Tip
Electronic housing
Sensing technology of HD
devices have a small
temperature drift and long
term offset drift due
advanced compensation
technology
© 2012 Data Sciences International
Reliability / Patency
DSI Pressure Sensing Catheter
Solid Tip Catheter
Non-Compressible Gel
Fragile latex membrane
Bloodstream
around pressure
sensing tip and
antithrombotic
treatment
prevents clothing
Long term
reliability of the
gel-tip tested and
proved in 1000+
chronic studies.
© 2012 Data Sciences International
Charging Challenges
No defined distance between charger & implant.
Energy transmission creates heat
Impact on temperature monitoring
Impact on animal behavior
Impact on animal welfare
Electrical field can create disturbances
More artifacts in signal recording
Makes refurbishment not obsolete
Necessary exchanges of catheter & lead material
Recommended annual factory re-calibration
© 2012 Data Sciences International
Battery Operation
Lifetime specified for continuous operation
Battery can be turn ON/OFF by a magnet,
Exchange by refurbishment procedure,
Advantages of Battery Devices:
Longer operation times without maintenance,
Known battery status,
Vendor warranted operation time,
No recharging required (impact on behavior and temperature)
No tissue irritations
Accurate temperature monitoring
Typical Battery Lifetime:
PA-C10 = 1.5month; CTA-F40 = 6 month; TA-F40 = 11month
© 2012 Data Sciences International
What’s the max. sampling rate?
Limited by Carrier Frequency
Typically 5x transmitter bandwidth
Sampling rate can be increased by interpolation
Example:
TF50-W-F2 for Sympathetic Nerve Analysis (SNA)
Bandwidth: 1kHz
Sampling rate: 5kHz
© 2012 Data Sciences International
Use of customized antennas
Transmission range restricted by energy consumption
Other influences on transmission ranges: Interferences
Metal shielding
Environmental noise
01
23
45
Small animal implant HD-X11
Large animal implant D70-EEE
Large animal implant PT Digital
Small animal implant HD-X11 Large animal implant D70-EEE Large animal implant PT Digital
Series1 0.2 1 5
Transmission range / m
© 2012 Data Sciences International
Behavioral data: running wheels, drinking and feeding activity, stimulus, etc.
Combine with video based Behavior Analysis. DSI data into Observer XT/Noldus
Combine with other Behavioral analysis systems: Laboras/Metris
Direct and Dynamic data exchange with Excel: scoring, develop homemade systems, Control output
Telemetry + Environmental + Behavioral data
© 2012 Data Sciences International
Telemetry + Environmental data
© 2012 Data Sciences International
Telemetry + Behavioral data
© 2012 Data Sciences International
Food intake behavior and CV monitoring
© 2012 Data Sciences International
CNS and Behavior Monitoring
© 2012 Data Sciences International
FAQ’s
How can I interface between Noldus behavior systems
and DSI telemetry?