Antimalarial PP presentation

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Antimalarial Artemisinin Michelle Atkins Javier Ramirez

Transcript of Antimalarial PP presentation

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Antimalarial Artemisinin

Michelle AtkinsJavier Ramirez

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What is Malaria?

A disease of the blood caused by the Plasmodium parasite.

100+ species of malaria parasite exists.

The most common is Plasmodium falciparum.

214M cases worldwide in 2015.

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Anti-Malarial Artemisinin Works faster against Uncomplicated

Malaria. Artemisinin is extracted from the

plant Artemisia Annua: Used in Chinese Traditional

Medicine.

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The Problem

Artemisinin Molecule

A. Annua plant

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Objectives

Find a stable source of affordable artemisinin.

Produce high yield biological strains of arteminisinic acid.

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Hypothesis

“The discovery of a plant dehydrogenase and a second cytochrome will provide an efficient

biosynthetic route to artemisinic acid”.

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The Amyris Approach

2. Automated DNA Assembly

1. Design Biosynthetic Pathway

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Automated DNA Assembly (Cont.)

Next Generation Sequencing

DNA Regions are assembled in vivo in yeast

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Hi-RYSE (Hyper-Integration for Rapid Yeast Strain Engineering)

Several new DNA designs

Single step strain conversion

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Pathway to Artemisinic Acid

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Synthesis of Artemisinin

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Purification Optimization

Feedback Controlled Pulse-Feed Extractive Fermentation Process Increases the Yield from 8 g/L to 28 g/l.

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Conclusion Plant Extraction

Mass Production Worldwide Supply

Yeast