Anti-Pollution

Introduction...................................................................................................................................................................................................................3Pollution - The Dirty Details......................................................................................................................................................................................................4Damaging Effects....................................................................................................................................................................................................................5-6Product Categories & Efficacy.................................................................................................................................................................................................7 Hair Care Efficacy................................................................................................................................................................................................................8 Hair Pollution Protection Assay....................................................................................................................................................................................9 Skin Care Efficacy.............................................................................................................................................................................................................10 Anti-Pollution Assay.......................................................................................................................................................................................................11 Hair Care Product Recommendations.....................................................................................................................................................................12 Skin Care Product Recommendations.....................................................................................................................................................................13References........................................................................................................................................................................................................14Sample Formulations..............................................................................................................................................................................................................15 UV Hair Protection Spray..............................................................................................................................................................................................16 Environmental Defense Lotion...................................................................................................................................................................................17 Restore & Protect Sulfate Free Shampoo...............................................................................................................................................................18 Age Fighting Micellar Water........................................................................................................................................................................................19

Table of Contents

Active Concepts has built itself upon three pillars: innovative technology platforms, market leading customer support, and products with a purpose. For almost two decades, Active Concepts has diligently embarked on an endeavor to develop complex, active ingredients that provide formulators with unmatched function, in order to meet and exceed the rapidly increasing consumer demand for more effective cosmetic technologies. Active Concepts prides themselves on their ability to identify market niches. We then design and develop unique, high quality actives through innovative biotechnology for the beauty industry.

Utilizing our extensive expertise in the science of fermentation has led us to innovate active ingredient concepts across our technology platforms. Our command of several technology platforms allows for the transformation of naturally derived compounds into truly functional bioactive cosmetic ingredients. Today, Active Concepts thrives in the rapidly evolving marketing environment by harnessing the latest technological advances to create novel products useful for brand differentiation.

At Active Concepts, utilizing our various technology platforms provides products with a purpose. Our actives are supported by biological concepts, clinical studies - in vitro and in vivo - and comprehensive marketing documentation to support our customers in positioning their products with the highest market impact. As new topics of discussion and development arise within the industry, we meet these research challenges head on to provide exceptional products across all industry platforms and stand behind them with efficient and effective testing methods.

In addition, flexible and dynamic usage of our technology platforms allows for innovative development through modular design. We offer customized product development for brand differentiation to provide unique ingredients that are unavailable to others on the market. Our alternate sourcing allows customers to optimize cost and decrease lead times while obtaining customized blends and products with preferred solvents, preservatives, and delivery systems.

Active Concepts offers innovation at the intersection of biochemistry and technology to inspire those creating tomorrow’s cosmetics.

Introduction

Pollution - The Dirty Details

Pollution is the introduction of contaminants into an otherwise natural environment. Ironically, as industrialization has improved quality of life around the globe tremendously, it has simultaneously increased the pollutants we live with on a daily basis. Air pollution, which may include microscopic particles of smoke, acid, and soot, can have a direct impact on health and wellness. As our first line of defense, the skin is consistently exposed to environmental stressors. Hair is also subject to extensive damage as pollution strips the hair of luster and strength leaving behind lifeless strands and an irritated scalp.

Regardless of where you live, pollution exposure is prevalent in rural and urban communities. UVB light from the sun causes the buildup of free-radicals on and under the skin leading to fine lines and wrinkles. Public water is another commonly overlooked source of pollution, capable of stripping the skin of its natural minerals. Severe or prolonged pollution links to DNA damage and cell mutation, which leads over time to uneven skin tone, accelerated aging, dark spots, and even cancer. Even when the stress environment is no longer present, DNA modification continues to occur following extended, unprotected exposure. If you do not live on a bustling city street, pollution can still affect daily life. Even if we never step outside again, we will still age thanks to indoor pollution. Household cleaners, furniture glue, some personal care products, dust, and volatile organic compounds from electronic equipment are just a few possible pollution providers. As the number of consumers living urban lifestyles continues to grow around the globe, it seems that many are turning toward beauty products to protect themselves from the impact of pollution. Worldwide opportunities are available for cosmetic brands as the effects of pollution are a prominent and ever-growing concern for conscious consumers. For years, the industry has successfully developed and marketed products that protect against UV induced skin damage. Now, scientists and formulators are focusing on warding off the effects of air pollution in major cities. Air pollution in Asia is at an all-time high as auto emissions, cigarette and industrial smoke have allowed cosmetics brands to expand their influence into skin and hair care that works to actively fight pollution. The Environmental Protection Agency is leading the charge against air pollution in the East. Now, Europe and North America are becoming as concerned about anti-pollution claims as Asian countries.

Damaging Effects

Oxidative stress and activated inflammatory responses are the main biological mechanisms involved with pollution damage. Oxidative stress occurs when the body's cells are not able to remove high levels of free radicals that are responsible for aging and tissue damage. Our cells utilize antioxidants to counteract free radicals, but when pollution exposure throws off the balance oxidative stress occurs.

There is a focus on reversing free-radical damage with the use of anti-pollution products: beyond traditional antioxidants like vitamins A and E - which only neutralize oxygen-based free radicals and not pollution’s nitrogen-based free radicals. Major anti-pollution claims for cosmetics emphasize film-forming, anti-oxidant, and anti-inflammatory capabilities.

Figure 1. Schematic representation of skin affected by particulate matter compared to skin protected with anti-pollution cosmetics.

The latest standard of pollution, the EPA’s PM 2.5 (Particulate Matter 2.5, air pollutants with a diameter of 2.5 micrometers or less) are tiny particles in the air that reduce visibility and have implications in overall air quality.

These fine particulate particles are often times smaller than the pores through which the skin breathes. They are capable of penetrating deeper layers of the epidermis causing inflammation, dehydration, and a cellular reaction that can lead to the loss of elasticity and firmness.

Although, even simple surface interactions can drastically alter the composition of the skin. Pollution can break down collagen and the skin’s lipid layer, impairing surface barrier function, causing dryness, acne, accelerated aging, and skin cancer.

Untreated skin susceptible to particulate matter Treated skin protected from particulate matter

The damaging effects of particulate matter 2.5, disturbing the skin barrier

Anti-pollution cosmetics offer protection from environmental particulate matter through film forming, antioxidant properties, and anti-inflammatory benefits

. ..

...

..

. ...PM 2.5

Damaging Effects

The popularity of anti-pollution products is on the rise as consumers around the world are living the urban lifestyle. The number of products launched containing anti-pollution claims will increase in coming years, and will have an impact on all beauty categories especially facial skincare, color cosmetics, haircare, soap and shower items.

Consumers with increased awareness of potential hair pollution are purchasing products aimed to fight UV rays and environmental pollution. Cosmetics manufacturers are turning to a range of ingredients to fight pollution’s negative effects. Plant extracts are especially popular due to consumer's current high demand for green formulations.

As pollution causes free radical damage and oxidative stress, accelerated skin aging is undeniable. Cellular processes are responsible for skin to heal and repair itself, but intracellular damage will impair these processes leading to fine lines, wrinkles, breakouts, and blemishes.

Figure 2. UV radiation and pollution influence on hair. Protected vs not protected.

Figure 3. Free radical's damaging effects cause oxidative stress on a cellular level.

Product Categories & Efficacy

Hair Care EfficacyThe deleterious effects of pollution in skin and hair care has become a new frontier for combative active ingredients. Environmental pollutants are results of automobile exhaust gas, industrial emissions, and even emissions from simple household chores such as cooking and cleaning. Hair is subject to such environmental aggressions as well as UV irradiation and, unlike skin, hair is vulnerable and lacks self-protection mechanisms.

Some pollution particles can be forty times smaller than a normal hair fiber. These particles will bind to the hair and penetrate into the follicles resulting in free radicals, oxidative stress, and cell damage. The hair pollution protection assay assesses the ability of our ingredients to protect the hair from the oxidative effects of air pollution.

Pollutant cigarette smoke is a suitable substance to perform testing, as it contains all key pollution components, such as reactive oxygen species (ROS), reactive nitrogen species, and electrophilic aldehydes. Reactive oxidants, as well as free radicals from cigarette smoke, are closely associated with oxidative stress and secondary oxidative events, such as lipid peroxidation. Hair care ingredients with anti-pollution capabilities protect the hair from environmental stressors, but at Active Concepts, we do not stop there. We offer multifunctional products to not only protect the hair, but also improve moisturization, render antioxidant benefits, reduce inflammation, and strengthen overall hair quality. Active ingredients work to protect and repair hair, one strand at a time.

Product to be tested applied to

hair swatches with 4 various testing groups.

Hair swatches exposed to

cigarette smoke in a smoke chamber.

Hair lipid peroxidation assessed for

each hair sample.

The MDA-TBA adduct

generated is quantified

colorimetrically.

Figure 4. Procedure for Hair Pollution Protection Assay.

Hair Pollution Protection AssayExposure to environmental pollution can result in dry, brittle hair with decreased strength and elasticity as well as an irritated scalp. Pollution may encourage sebum production on the scalp resulting in oily hair strands and clogged pores. Long-term exposure to pollution conditions increase the likelihood of excess hair loss as well as degradation of the hair texture resulting in rough, broken, and lackluster hair strands.

00.20.40.60.8

11.21.41.6

Untreated Control Shampoo/ConditionerControl

2.0% ACB PisumSativum Peptide in

Shampoo andConditioner

2.0% ACB PisumSativum Peptide

Saturation with Rinse

2.0% ACB PisumSativum Peptide

Saturation withoutRinse

nmol

/µL

Malonaldehyde Lipid PeroxidationAverage MDA Values

Figure 5. Average MDA values for 16810 ACB Pisum Sativum Peptide.

Evaluating the lipid peroxidation of hair can determine the ability of an ingredient to provide pollution protection. Lipid peroxidation refers to the oxidative degradation of lipids. Lipids keep hair hydrated and protected by providing a coat or shield on the hair strands. In this process, free radicals take electrons from the lipids, resulting in cell damage. Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms reactive aldehydes such as MDA, or malondialdehyde, as natural byproducts. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. In the study, evaluation of the active ingredient determines its effect on the inhibition of lipid peroxidation of hair samples exposed to air pollution. Lower levels of MDA correlate with efficient pollution protection for hair.

Skin Care EfficacyIn order to lead the market in customer service and continue to produce products with a purpose, we must continue to effectively support each level of the personal care industry with value added benefits for finished anti-pollution goods. Consumer awareness has led to the desire for ingredients with multi-functional protection and the demand for efficacious materials.

Product to be tested applied

to the skin. Experimental, control, and untreated

areas.

Micronized charcoal added to each testing area to mimic

particulates found in

polluted air.

Each area is washed 5 times with deionized

water.

Images taken pre- and post-

wash are compared and

analyzed.

Figure 6. Procedure for Anti-Pollution Assay.

The role of pollution in the appearance of the premature wrinkles and age spots has become a new frontier in anti-aging active ingredients. While we have known about the harmful effects of pollution on our health for years, new research indicates air pollution plays a detrimental role in extrinsic aging. Carbon and metal micro particles found in polluted air embed in the dermis causing oxidative stress, initiating the inflammatory cascade leading to the breakdown of collagen, elastin, and other structural components in the skin. Additionally, polyaromatic hydrocarbons overstimulate the aryl hydrocarbon receptors on keratinocytes and melanocytes resulting in the hyperpigmentation and the appearance of age spots. Providing a physical barrier will prevent embedment of carbon particles, thus reducing the signs extrinsic aging. The pollution protection assay assesses the ability of our ingredients to provide immediate protection from carbon air pollution.

Standard Assay: Inhibition of PM 2.5 The latest standard of pollution, the EPA’s PM 2.5 (Particulate Matter 2.5, air pollutants with a diameter of 2.5 micrometers or less) are tiny particles in the air that reduce visibility, and have implications in overall air quality. These fine particulate particles are many times smaller than the pores through which the skin breathes. They are capable of penetrating deeper layers of the epidermis causing inflammation, dehydration, and a cellular reaction that can lead to the loss of elasticity and firmness. Although even simple surface interactions can drastically alter the composition of the skin, pollution can break down collagen and the skin’s lipid layer. This can impair surface barrier function causing dryness, acne, accelerated aging, and even skin cancer. Figures 7-12 depict the results from the pollution protection assay for 16586-Phytofuse Renew®. Images undergo color analysis to depict results in optical density values and pigmentation histograms.

Anti-Pollution AssayPreventing adherence of the micronized charcoal residue indicates the product’s ability to provide barrier protection against everyday air pollution. At the end of the day, we want to be able to wash off any pollution particles or contamination that we may have encountered during the day. Pollution protection ingredients offer a defensive layer against unwanted pollution to keep skin healthy and youthful.

Figure 7. Phytofuse Renew® Pre-Wash. Figure 8. Phytofuse Renew® Post-Wash.

Figure 10. Untreated Control Pre-Wash. Figure 11. Untreated Control Post-Wash.

Figure 12. Untreated Histograms Quantifying Charcoal Residue.

Figure 9. Phytofuse Renew® Histograms Quantifying Charcoal Residue.

Hair Care

Product Code & Name INCI Name Mechanism Multifunctional Benefits

22045 Active.Lite® HairPolyquaternium-80 & Water & Hydrolyzed Pea Protein & Selaginella Lepidophylla Extract

Ionically binds to hair cuticle offering long-term protection from harsh hair bleaching processes

• Chemically Resilient Material• Utilizes Matrix-style Scaffolding• Strengthens Hair

16810 ACB Pisum Sativum Peptide Pisum Sativum (Pea) PeptideEffectively conditions and moisturizes strands through its superior film forming and deep conditioning properties

• Improves Combability• Great for Hair and Skin• Antioxidant Protection

16586 Phytofuse Renew® Selaginella Lepidophylla Extract

Imparts protective and moisture-retentive benefits associated with the secondary metabolites produced by the Resurrection Plant

• Improves Barrier Function• Natural silicone replacement• Antioxidant Protection

16882 Phytofuse Rejuvenate® Salvia Hispanica Seed ExtractIsolated polysaccharides from Chia create a synthetic scaffolding that promotes wound healing and aid in cellular proliferation

• Natural Film-Former• Pro-Collagen Synthesis• Antioxidant Protection

20342PF ACB Yerba Santa Glycoprotein PF Lactobacillus/Eriodictyon Californicum Ferment Extract

Mucopolysaccharides and glycoproteins work to counteract excessive dryness and deliver lasting moisture

• Enhance Aesthetics• Intense Moisturization• Hydration & Lubricity

20742 AcquaSeal® Coconut Cocos Nucifera (Coconut) Fruit ExtractFractionated lipids seal in moisture and transform the skin into a luxuriously soft, hydrated surface.

• Moisturizing• Perceivable Sensorial Attributes• Antioxidant Protection

The cuticle is the outermost layer of the hair, which is formed by tightly packed scales. The ideal position of the cuticle for maximum defense against pollution and moisture retention is laying flat and sealed. When the cuticle becomes lifted due to thermal, mechanical, chemical, or environmental stress from pollution, it becomes prone to additional damage, lacks moisture, and requires additional protection.

Skin Care

Product Code & Name INCI Name Mechanism Multifunctional Benefits

10550 ABS Moringa Pterygosperma Seed Extract G Glycerin & Moringa Oleifera Seed Extract

Capitalizes on the benefits associated with moringa seeds while offering a protein free product that effectively increases collagen production while providing strong antioxidant attributes

• Increases Barrier Protection• Nourishes and Conditions Skin• Antioxidant Protection

20431PF ACB Tonka Bean Bioferment PF Lactobacillus/Dipteryx Odorata Seed Ferment Filtrate

Emits visible blue light to counteract dulling effects of other wavelengths and increase luminescence for healthy and revitalized skin

• Antioxidant Protection• Promotes Collagen Synthesis• Improves Moisturization

20342PF ACB Yerba Santa Glycoprotein PF Lactobacillus/Eriodictyon Californicum Ferment Extract

Mucopolysaccharides and glycoproteins work to counteract excessive dryness and deliver lasting moisture

• Improves Barrier Function• Intense Moisturization• Antioxidant Protection

21005PF AC Biopolymer Chia PF Coconut Acid & Salvia Hispanica Seed Extract

Modified protein with a lipid moiety that disrupts the surface tension of sebum on the skin by binding to excess sebum.

• Sebum Control• Film Former• Antioxidant Protection

20742 AcquaSeal® Coconut Cocos Nucifera (Coconut) Fruit ExtractFractionated lipids seal in moisture and transform the skin into a luxuriously soft, hydrated surface

• Moisturizing• Improves Barrier Function• Reduces Transepidermal Water Loss

16882 Phytofuse Rejuvenate® Salvia Hispanica Seed ExtractIsolated polysaccharides from Chia create a synthetic scaffolding that promotes wound healing and aid in cellular proliferation

• Anti-Inflammatory• Pro-Collagen Synthesis• Antioxidant Protection

Anti-pollution cosmetics are essential for protecting the skin from free radical damage and other extrinsic, environmental stressors. It is well documented that UV irradiation exacerbates the formation of hyperpigmentation (age spots) on the skin, while other irritants can cause inflammation, poor collagen synthesis, and transepidermal water loss. Using anti-pollution cosmetics to help protect the skin from extrinsic stressors caused by pollution can in turn help to maintain a healthy and balanced complexion.

1. E, Moeglin. et al. 2014. Skincare fights back to protect against pollution.

2. M, Yeomans. et al. 2014. Mintel confirms anti-pollution is dominating Asia beauty claims.

3. Clarins. et al. 2015. Don’t let pollution get under your skin! Clarins Mag.

4. S, Pitman. Et al. 2015. P&G looks to expand its anti-pollution skin care offering.

5. J, Mills. et al. “Why beauty brands are publishing Anti-pollution Claims.” Gcimagazine.com.

6. Mintel et al. 2014. Anti-pollution claims on beauty products in Asia Pacific grow by 40% in two years.

7. D, Utroske. et al. 2014. Olay’s research in Beijing shows the effects of air pollution on skin health.

8. A, McDougall. et al. 2015. Opportunities lie ahead as anti-pollution trend continues.

9. F, Bernardin. et al. 2015. China vs. Asia: The different faces of anti-ageing.

References

Sample Formulations

Trade Name INCI Name % VendorPhase A

Water Water (Aqua) 81.88 Local

Zemea® Propanediol Propanediol 1.00 DuPont, Tate, & LyleKeltrol® CG Xanthan Gum 0.12 CP Kelco

ACB Tonka Bean Bioferment PF Lactobacillus/Dipteryx Odorata Seed Ferment Filtrate 2.00 Active Concepts

Phytofuse Renew® Selaginella Lepidophylla Extract 4.00 Active ConceptsIsostearamidopropyl Morpholine Lactate Isostearamidopropyl Morpholine Lactate 1.00 ParchemCetiol® 767 PEG-6 Caprylic/Capric Glycerides 1.00 BASFAC Keratin Amino Acids PF Keratin Amino Acids 1.00 Active ConceptsPhase B Citric Acid Citric Acid (10%) Q.S. Sigma-Aldich

Leucidal® SF Complete Lactobacillus Ferment & Lactobacillus & Cocos Nucifera (coconut) Fruit Extract 4.00 Active Micro Technologies

1. Add xanthan gum to the propanediol. Mix Well.2. Heat deionized water to 60°C.3. Add xanthan gum/propanediol mixture. Allow to mix.4. Add all the remaining ingredients individually with mixing.5. Allow to cool while mixing.6. Add Phase B. Allow to mix.7. Adjust pH to 5.5 with a 10% Citric Acid solution if necessary.

Procedure

UV Hair Protection Spray

Trade Name INCI Name % VendorPhase AWater Water (Aqua) 42.40 LocalLiposorb® L-20 Polysorbate 20 1.00 VantagePhase BGlycerin U.S.P. Natural 96% Glycerin 4.00 Cognis Corp.Keltrol® CG Xanthan Gum 0.20 CP KelcoPhase CDermol 25B C12-15 Alkyl Benzoate 23.15 Alzo International Inc.

TNSS75MZCMZinc Oxide (And) Ethylhexyl Methoxycrylene (And) C12-15 Alkyl Benzoate (And) Polyhydroxystearic Acid (And) Hydrogen Dimethicone

9.80 Kobo Products

Phytomulse® Coconut Cetearyl Alcohol & Glyceryl Stearate & Coceth-20 3.45 Active ConceptsAcquaSeal® Coconut Cocos Nucifera (coconut) Fruit Extract 0.50 Active ConceptsPhase DMSS-500W Silica 3.00 Kobo Products

Sepigel™ 305 Polyacrylamide (And) C13-14 Isoparaffin (And) Laureth-7 1.00 Seppic

Phase EPhytofuse Rejuvenate® Salvia Hispanica Seed Extract 2.00 Active ConceptsACB Wakame Bioferment Advanced Undaria Pinnatifida Cell Culture Extract 1.00 Active ConceptsAC DermaPeptide Tightening PF Hydrolyzed Wheat Protein 2.00 Active ConceptsAC CytoPure PF Crypthecodinium Cohnii Extract 2.50 Active Concepts

ACB Bio-Chelate VC Ascorbic Acid/Orange/Citrus Limon/Citrus Aurantifolia Polypeptides 2.00 Active Concepts

Phase F

Leucidal® SF Complete Lactobacillus Ferment & Lactobacillus & Cocos Nucifera (coconut) Fruit Extract 2.00 Active Micro Technologies

1. Combine Phase A into main beaker and heat to 70°C.2. In a separate container, combine Phase B ingredients into a slurry.3. Add Slowly to Phase A while mixing.4. Add Phase C to main beaker under homogenization.5. In a separate container, combine Phase D ingredients and heat to 70°C.6. Add Phase D to main beaker while cooling to 45°C.7. Once cooled, add Phase E and F to main beaker.

Procedure

Environmental Defense Lotion

Trade Name INCI Name % VendorPhase A

Water Water (Aqua) Q.S. Local

AC Milk Hydrolysate SF Hydrolyzed Milk Protein 1.00 Active ConceptsPhytofuse Rejuvenate® Salvia Hispanica Seed Extract 2.00 Active Concepts

AC Kerazyme® Protect Hydrolyzed Keratin & Bambusa Arundinacea Leaf Extract & Trametes Versicolor Extract 5.50 Active Concepts

Phase BGlycerin Glycerin 3.00 Dow ChemicalKeltrol® Xanthan Gum 1.00 CP KelcoPhase CWheat Germamidopropyl Betaine Wheat Germamidopropyl Betaine 20.00 Mackam WGBAC Foaming Soy PF Sodium Cocoyl Hydrolyzed Soy Protein 15.00 Active ConceptsCoco/Sunfloweramidopropyl Betaine Coco/Sunfloweramidopropyl Betaine 10.00 Mackam WGBPhase DAC Keratin Hydrolysate 30 PF Hydrolyzed Keratin 4.00 Active ConceptsAC Silk Amino Acids PF Silk Amino Acids 4.00 Active Concepts

ACB Yerba Santa Glycoprotein PF Lactobacillus/Eriodictyon Californicum Ferment Extract 2.00 Active Concepts

Active.Lite® Hair Polyquaternium-80 & Water & Pisum Sativum (Pea) Peptide & Selaginella Lepidophylla 5.00 Active Concepts

Phase ELeucidal® Liquid Leuconostoc/Radish Root Ferment Filtrate 4.00 Active Micro Technologies

AMTicide® Coconut Lactobacillus & Cocos Nucifera (Coconut) Fruit Extract 4.00 Active Micro Technologies

Citric Acid 50% Solution Citric Acid 0.20 Spectrum Chemical

1. Mix and charge water into main beaker and begin propeller mixing. A vortex should form.2. Charge remaining Phase A ingredients and begin heating to 70°C. Mix until homogenous.3. Pre-mix Phase B ingredients then add to main beaker and mix until homogenous.4. Switch to sweep mixing. At 75°C add each Phase C ingredient individually to the main beaker.5. Allow each ingredient to disperse completely before adding the next. Begin force cooling.6. At 50°C, add Phase D ingredients individually to the main beaker and mix until homogenous.7. Once mixture is below 40°C add Phase E ingredients mixing thoroughly.8. Adjust pH if necessary using Citric Acid 50% solution.

Procedure

Restore & Protect Sulfate Free Shampoo

Trade Name INCI Name % VendorPhase AWater Water (Aqua) 72.25 LocalTetrasodium EDTA Tetrasodium EDTA 0.05 Spectrum ChemicalUSP Glycerin Vegetable Glycerin 3.00 Spectrum ChemicalCocamidopropyl Betaine Cocamidopropyl Betaine (35%) 6.00 BASF

AMTicide® Coconut Lactobacillus & Cocos Nucifera (Coconut) Fruit Extract 4.00 Active Micro Technologies

Leucidal® Liquid SF Lactobacillus Ferment 4.00 Active Micro TechnologiesPhytofuse Renew® Selaginella Lepidophylla Extract 1.00 Active ConceptsAC Soluble Elastin 10 PF Hydrolyzed Elastin 2.00 Active ConceptsACB Pisum Sativum Peptide Pisum Sativume (Pea) Peptide 2.00 Active ConceptsACB Phyto-Biotics Bifidus Lactobacillus Bifidus Lysate Extract 5.00 Active ConceptsPolysorbate 20 Polysorbate 20 0.30 Spectrum ChemicalPolysorbate 80 Polysorbate 80 0.30 Spectrum ChemicalFragrance Fragrance (Optional) 0.10 N/ASodium Hydroxide Sodium Hydroxide (25%) Q.S. Spectrum Chemical

1. Charge water to vessel.2. Using appropriate mixing, add ingredients in descending order, waiting for each to be dissolved before adding the next.3. If fragrance is desired, pre-mix Polysorbate 20, Polysorbate 80, and fragrance, add to batch.4. Adjust pH to 5.0 with NaOH solution.

Procedure

Age Fighting Micellar Water

Active Concepts LLC, AsiaKaohsiung, Taiwan

www.activeconceptsllc.comTel + 886 73599900

info@activeconceptsllc.com.tw

Active Concepts JapanTokyo, JAPAN

www.activeconceptsllc.com Tel +813 62311022

info-tokyo@activeconceptsllc.com

Active Concepts S.r.l. Milano ITALY

www.activeconcepts.it Tel +39 02 90360719

info@activeconcepts.it

Active Concepts, LLCLincolnton, NC. USA

www. activeconceptsllc.comOffice: +1 (704) 276 7100

info@activeconceptsllc.com