Annex A M. PHIL PROGRAMME IN MOLECULARâ€BIOLOGY, VIROLOGY and IMMUNOLOGY
Transcript of Annex A M. PHIL PROGRAMME IN MOLECULARâ€BIOLOGY, VIROLOGY and IMMUNOLOGY
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Annex A
M. PHIL PROGRAMME IN MOLECULAR”BIOLOGY, VIROLOGY and IMMUNOLOGY
NATIONAL CENTRE OF EXCELLENCE IN VIROLOGY AND IMMUNOLOGY
For M. Phil Degree, 24 credit hours course work +06 credit hours for one year of original
research work and for Ph.D degree additional 18 credit hours course work + 30 credit hours of
original research work will be required.
M Phil. Semester-I Course No. Course Title Credit Hours Status
MVI-800 Gen & Mol. Virology 3 Core
MVI-810 Cell and Mol. Biology 3 Core
MVI-930 Biosafety and Bioethics 3 Core
MVI-830 Gen & Mol. Immunology 3 Core
Semester –I Total Credits 12
M.Phil. Semester-II Course No. Course Title Credit Hours Status
MVI-804 Bacteriophage Biology 3 Core
MVI-860 Viral Pathology 3 Core
MVI-850 Signal Transduction 3 Core
MVI-901 Bioinformatics 3 Elective
MVI-xxx Elective – II 3 Elective
Semester – II Total Credits 12
MVI-899 M Phil. Thesis Research 6 Compulsory
Total Credits 30
List of Elective_II. Courses MVI-810 Recombinant DNA techniques (3 Credit Hours) MVI-820 Microbiology (3 Credit Hours) MVI-834 Immune Pharmacology (3 Credit Hours) MVI-880 Molecular & General Toxicology (3 Credit Hours) MVI-920 Lab. Diagnostic Techniques (3 Credit Hours)
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Annex B
PH D COURSE WORK IN MOLECULAR”BIOLOGY, VIROLOGY and IMMUNOLOGY
NATIONAL CENTRE OF EXCELLENCE IN VIROLOGY AND IMMUNOLOGY PhD. Semester-I Course No. Course Title Credit Hours Status
MVI-810 Adv. in Mol. Biology 3 Core
MVI-801 Adv. in Virology 3 Core
MVI-xxx Elective – I 3 Elective
Scientific writing and presentation skills Non credit course
Total Credits 9
PhD. Semester-II Course No. Course Title Credit Hours Status
MVI-851 Medical Genetics 3 Core
MVI-870 Vaccinology 3 Core
MVI-xxx Elective – II 3 Elective
Total Credits 9
MVI-999 PhD Thesis Project: 30
Total Credits 48 (excluding MPhil Credits)
Elective Courses: MVI-840 Trends in Pharmacology MVI-852 Immunogenetics MVI-861 Molecular Mechanisms of Disease MVI-910 Stem Cell Studies MVI-940 Experimental Therapeutics MVI-950 Plant viruses and diseases
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Annex C
NCVI Core Curriculum MPhil/PhD program
Following is the list of course that will be initially offered in NCVI. With the induction of more students and faculty, additional course will be offered and some will be modified. The changes will be made periodically by the academic body of NCVI and sent to Academic and Examination (A & E) body of NUST for final approval.
Major Courses CODE List
Serial No Course Code Course Title .
1 MVI-80 Virology
2 MVI-81 Molecular and Cell Biology
3 MVI-82 Microbiology
4 MVI-83 Immunology/Immune disorders
5 MVI-84 Pharmacology (Viral, Immune etc)
6 MVI-85 Medical Genetics/ Targeted Gene Therapy
7 MVI-86 Viral Pathology
8 MVI-87 Vaccinology
9 MVI-88 Metabolism/Toxicology
10 MVI-89 Biotechnology/Fermentation
11 MVI-90 Bioinformatics
12 MVI-91 Stem Cell Studies
13 MVI-92 Lab Diagnostic Techniques/Instrumentation
14 MVI-93 Biosafety/Bioethics
15 MVI-94 Experimental Therapeutics
16 MVI-95 Plant viruses and diseases
Each course has further Subdivisions as listed below.
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Serial No
Course Code Course Title .
1
MVI-80
Virology
CODE CONTENTS 800 GENERAL VIROLOGY 801 Basic Concepts in Virology 802 ADVANCES IN VIROLOGY
803 MECHANISTIC VIROLOGY
804 Bacteriophage Biology 805
2
MVI-81
Molecular Biology/Cell Biology
CODE CONTENTS 810 MOLECULAR BIOLOGY
811 CELL BIOLOGY-I
812 CELL BIOLOGY -II
813 GENOMICS AND PROTEOMICS
814 815
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MVI-82
Microbiology
CODE CONTENTS
820 MOLECULAR MICROBIOLOGY_1
821 MOLECULAR MICROBIOLOGY_2
822 823 824 825
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MVI-83
Immunology/Immune Disorders
CODE CONTENTS 830 CLINICAL IMMUNOLOGY 1 831 CLINICAL IMMUNOLOGY 2 832 ANTIVIRAL IMMUNOLOGY
833
834 835
5
MVI-84
Pharmacology (Viral, Immune etc)
CODE CONTENTS 840 PHARAMACOLOGY
841 842 843 844 845
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MVI-85
Medical Genetics/ Targeted
Gene Therapy
CODE CONTENTS 850 GENERAL GENETICS
851 MEDICAL GENETICS
852 IMMUNOGENETICS
5
853 GENETHERAPY
854 855
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MVI-86
Microbial Pathology
CODE CONTENTS 860 Viral Pathology 861 Molecular Mechanisms of Disease 862 863 864 865
8
MVI-87
Vaccinology
CODE CONTENTS 870 VACCINOLIOGY
871 872 873 874 875
9
MVI-88
Metabolism/Toxicology
CODE CONTENTS 880 MOLECULAR TOXICOLOGY
881 882 883 884 885
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MVI-89
Biotechnology/Fermentation
CODE CONTENTS 890 BIOTECHNOLOGY
891 892 893 894 895
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MVI-90
Bioinformatics
CODE CONTENTS 900 BIOSTATISTICS
901 BIOINFORMATICS
902 903 904 905
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MVI-91
Stem Cell Studies
CODE CONTENTS 910 STEM CELL STUDIES 911 912 913
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914 915 916
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MVI-92
Lab Diagnostic
Techniques/Instrumentation
CODE CONTENTS 920 LAB DIAGNOSTICS/INSTRUMENTATION
921 922 923 924 925
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MVI-93
Biosafety/Bioethics
CODE CONTENTS 930 ISO-9007 931 932 933 934 935
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MVI-94
Experimental Therapeutics
CODE CONTENTS 940 EXPERIMENTAL THERAPEUTICS
941 942 943 944 945
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MVI-95
Plant Viruses and Diseases
CODE CONTENTS 950 PLANT VIROLOGY
951 STRATEGIES TO COMBACT PLANT VIRUSES
952 953 954 955
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MVI-800: GENERAL VIROLOGY (3 CREDIT HOURS)
COURSE CONTENTS: This is an introductory level course that describes the history of virology,
development of the concepts of viruses, and various biological and molecular aspects of bacterial,
animal and plant viruses. The course is divided into 3 parts.
COUSRE CONTENTS:
200 years of discoveries
General aspects of virology
Viral Classification and Structure
Modes of infection
Cellular Models of virus propagations
Replication and pathogenesis in a comparative fashion.
Host-virus interactions
Transformation and oncogenesis
Immunopathology
Host defense mechanisms
Antiviral pharmacology and applied virology
Bacteriophages
Plant viruses and their associated diseases
What is next for Virology?
MVI-800: BASIC CONCEPTS IN VIROLOGY (3 CREDIT HOURS)
LEARNIG OBJECTIVES: Viral life cycle, mode of replication and infection will be discussed.
Cytopathic effects of viral infection, lytic and lysogenic infection and viral gene expression and host
defenses will be discussed.
COURSE CONTENTS:
Introduction to Bacteriophages and viruses
Virus Genomes/ Viral Genetics: Linear, Circular, DNA or RNA
Viral Infection: Attachment, penetration, uncoating
Viral Gene Expression: Early and late gene expression
Viral Replication: Lytic and latent life cycles
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Viral Pathogenesis: Virulence, Cytopathic effects, Host responses
Virus spread through insect vectors
Phenotype mixing and pseuodtypes
Viral Transformation
Prions, Viroids and Virosoids
RNA transforming viruses
DNA transforming viruses
Host Defense Mechanisms
Apoptotic or Anti-apoptotic Viruses
Immune activation
Anti-Viral Pharmacology
Antiviral drugs
BOOKS RECOMMENDED:
Lytic mode of lambda development by D. Freidman and M. Gottesman, cold spring harbor
laboratory, cold spring harbor, N.Y.
Single stranded DNA by R.L. Sinsheimer.
Principles of Virology by S.J. Flint.
Fields Virology by B. Fields
Principles of Virology by Knipe.
Principles of Virology by Alan J. Cann.
MVI-802: ADVANCES IN VIROLOGY (3 CREDIT HOURS)
LEARNING OBJECTIVES: Details of the life cycle of different classes of viruses will be
thought. This course is deigned in such as way that students will be able to distinguish target specific
pathways of various classes of viruses. By understanding the common factors that contribute to
diseases caused by viral infections, it will help students to develop strategies to prevent or resolve
such infections.
COURSE CONTENTS
Hepresvirues (HSV-1, HSV-2, HCMV, EBV, PRV, VZV, HSV-6, HSV-8)
Retroviruses (HIV-1, HIV-2, HTLV-1, HTLV-2)
Defective Retroviruses and Endogenous Retrotransposons
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Hepatitis viruses (HAV, HBV, HCV, HDV, HEV, HGV)
Entroviruses (Coxsackieviruses, Echovirus, Hepatitis A, Norwalk and Norwalk like viruses,
Reovirus, and Rotavirus)
Influenza and cold viruses
Neurotropic viruses [lymphocytic choriomeningitis virus (LCMV) and Measles virus (MV)]
Adenoviruses (Ad-1, Ad-2, Ad-6)
Picornaviruses (Poliovirus, rhinovirus)
Togaviruses (Rubella, Yellow fever, encephalitis)
Polyoma viruses (SV40, JC, and Papilloma)
Coronaviruses
Parvoviruses (ssDNA)
Reoviruses (dsRNA)
Poxviruses
Plus stranded RNA viruses vs. Negative-stranded RNA viruses
Yeast viral like particles (VLPs) (Ty-1, Ty-2, Ty3)
Insect viruses (Dengue, Tick born)
Primate viruses (Ebola and Marburg)
Avian viruses (Bird flu)
BOOKS RECOMMENDED:
Hepatitis B and C carrier to cancer by Sarin.
Retroviruses by Coffin and John M.
Human Retroviruses by Bryan R.
Interaction by retroviruses and Herpes viruses by H. J. Kung.
Human Papilloma viruses by D.J.McCance.
Human Papilloma viruses by Clare (EDT) Davy.
The coronaviridae by Stuart G. Siddenn.
Molecular Biology of Hepatitis B virus by Koshy.
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MVI-803: MECHANISTIC VIROLOGY (3 CREDIT HOURS)
LEARNING OBJECTIVES: Details of the potential mechanism(s) of viral replication and
transcription and translation control will be thought. This course is deigned in such as way that
students will be able to design antiviral approaches that will target specific pathways of viral life
cycle.
COURSE CONTENTS
Oncogenic viruses and potential role of oncogenes
Neurotropic viruses and neurovirulence
Balance between neuroprotection and immunopathogenesis
Mechanism(s) of latency
Packaging/Encapsidation of viral genome
Mechanisms of replication of RNA viruses
Mechanisms of replication of DNA viruses
Control for viral RNA processing
Control of RNA transport and stability
Mechanism of antiviral therapy
Cell to cell spread of viruses
Glycobiology relevant to viral pathogenesis and control
Intracellular Transport mechanism(s) of mature viral particles
Signal transduction pathways perturbed by viral infection and/gene expression
Cellular and animal model of virus propagation
Use of viral vectors as gene therapy
Development of surrogate/pseudo type models for viral genes expression
LAB PRACTICALS
Bacteriophage Isolation (M13, lambda, Mu)
Transduction vs. transposition assay
Purification of replicative forms of M13 ssDNA
Viral isolation and Plaque Assay (Hepresviruses, HSV-1 and HCMV)
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MVI-804: BACTERIPHAGE BIOLOGY (3 CREDIT HOURS)
LEARNING OBJECTIVES: Historical overview of pages will be presented. The structural
organization, genetics, attachment, penetration and their replication will be discussed. The
bacteriophages of marine bacteria constitute the highest amount of biodiversity in nature which will
be compared with terrestrial bacteriophage ecology. The use of bacteriophages as therapy to cure
multi-drug resistant bacteria, for cleaning the contaminated polluted rives/garbage will be discussed
COURSE CONTENTS:
General properties of phages
Historical overview of phages
Classification of gram positive and gram negative phages
Large DNA, small DNA and RNA phages
Attachment and Penetration
Synthesis of protein and nucleic acids
Bacteriophage lysis: Mechanism and regulation
Phages and transposable elements (Mu Biology)
Lambda phage
T3, T7 and T4 phages
P1 (Cre lox) system
Defective phages and phage-like objects
Evolution and natural biology of phages
Evolutionary conservation and relation to eukaryotic viruses
Relation of bacteriophages to bacterial ecology
The health value of bacteriophages (Phage Therapy)
Synthetic bacteriophages
Use of bacteriophages in Physics (Nano wires and electrodes)
Biocide bacteriophages for environmental surfaces
Bacteriophages of industrial importance
Control of bacteriophage contamination
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MVI-810: MOLECULAR BIOLOGY (3 CREDIT HOURS)
LEARNING OBJECTIVES: This course will discuss basic principles of molecular biology,
structures of macromolecules with emphasis on the mechanisms that dictate the fundamental
processes for the transfer of DNA->RNA-> protein.
COURSE CONTENTS:
Structure and origin of RNA and DNA
Molecular structure and organization of Genes, Genome and Chromosomes
Gene Transcription
Regulation of Transcription Initiation, role of GTF and TSFs
DNA Replication
DNA Recombination (site specific and non-homologous)
RNA Processing, Nuclear Transport, and Post-Transcriptional Control
Control of Translation
Selection of mRNAs to be translated
mRNA degradation and interference
Protein activity: Post-translational modifications, activation, localization, and degradation
Protein structure and function
LAB PRACTICALS
Nucleic Acid Biochemistry and Analysis:
DNA isolation (Plasmid and Chromosomal)
Agarose and Polyacrylamide Gel Electrophoresis
Electrophoresis Mobility Shift Assays (EMSA)
Chromatography
Spectrophotometer Techniques
Molecular Cloning and subcloning
Vector preparation, restriction mapping
Polymerase chain reaction (PCR) of a target gene
Agarose gel electrophoresis of gene PCR product
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Analysis of restriction digest, ligation into a vector
Transformation to E. coli and yeast
Selection and Screening, Plasmid preparation and large scale purification
Southern Blot vs. Northern blot analysis
DNA sequencing and Alignments-Review bioinformatics tools
Protein Translation: Open Reading Frames
BOOKS RECOMMENDED
Molecular Biology of the Gene by Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, and
R. Losick, Eds., (2003). (5th edition) New York, Benjamin Cummings ISBN 0-8053-4635-X
The Cell by Bruce Albert and Dennis Bray, (3rd Edition), Garland Publishing Inc, New York and
London Gene VIII By Lewin Benjamin Eds 2004. Oxford University press, Inc, New york.
Biochemistry by Victor L. Davidson, Donald B. Sittman. 3rd Edition, 1993, Harwal Publication Co.
Cell and Molecular Biology by Gerald Karp.1996 John Willey and Sons, Inc. London.
Genetics by Peter J. Russel. 5th Ed. 1997, Benjamin-Cummings Publishing Company.
MVI-811: CELL BIOLOGY-I (3 CREDIT HOURS)
LEARNING OBJECTIVES: This course is designed to help students understand workings of the cell structure and function at
cellular and molecular level. In this course molecular basis of cell structure and function is
discussed which will provide students a detailed account of functional physiology of the cells.
COURSE CONTENTS:
Introduction
Cell Organization
Cell Architecture
Membrane Structure and Function
Bio Transport
Vesicular Transport
Transport Signals
Nuclear Transport
Bio Energetic
Mitochondrial Energy Conversion
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Chloroplast Energy Conversion
Cytoskeleton
Cell Shape
Cell Contractility
Cell to cell Communication
Electrochemical Signaling
Synaptic and Sensory Transduction
Biochemical Signaling
Receptor Ligand Interactions
Second Messengers
Signaling Cascades
Cell Cycle and Apoptosis
Phases of Cell Cycle and Cell Division
Regulation of Cell Growth and Death
Specialized Cell Systems
BOOKS RECOMMENDED:
Gene VIII By Lewin Benjamin, Eds 2004. Oxford University press, Inc, New york.
Molecular Biology of the Gene by Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, and
R. Losick, 5th Ed. 2003. New York, Benjamin Cummings ISBN 0-8053-4635-X
Molecular Biology of the Cell by Bruce Albert and Dennis Bray, 3rd Ed. Garland Publishing Inc,
New York and London.
Biochemistry by Victor L. Davidson, Donald B. Sittman. 3rd Ed. 1993, Harwal Pub Co.
Cell and Molecular Biology by Gerald Karp.1996 John Willey and Sons,Inc. London.
MVI-812: MEMBRANE TOPOLOGY & SIGNAL TRANSDUCTION (3CREDIT HOURS)
LEARNING OBJECTIVES:
This course is designed to help students understand workings of the cell structure and function at
cellular and molecular level that various viruses utilize in their life cycles. In this course molecular
basis of cell structure and function is discussed which will provide students a detailed account of
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functional signaling cascade physiology of the cells. Furthermore, this course will discuss the key
components of the immune system at cellular and molecular level.
COURSE CONTENTS:
Cell Adhesion and ECM
Cell adhesion molecules and protein targeting
Collagen Matrix and Non-collagen Matrix
Metastasis
Protein sorting
An overview of protein targeting
Peroxisomal protein targeting
Secretary protein targeting
Membrane proteins
Functions of cell surface receptors
Post-translation modifications
Golgi protein sorting
Vesicular Transport
Cytoskeleton and force generation
Actin dynamics and Actin myosin interaction and regulation
Microtubule dynamics (dynamic instability model)
MAPS and microtubule motors
Cilia and flagella structure and movement
Pathways of intracellular signal transduction
P38 MAPK, JNK, ERK pathways
RAS and RAF pathways
Oxidative stress and anti-stress signaling (GST, MnSOD)
GTPase and their role in cancer
Ion channels expression, regulation and its relations to diseases
Purinergic signaling (p2X and p2Y)
PKA, PKB (AKT) PKC pathways
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JAK/STAT and TGF-ȕ/smad pathways
NF-せB signaling
Hedgehog Wnt and Notch pathways
G-Protein coupled membrane receptors signaling
Steroid hormones and nuclear receptors signaling
Neurotransmitters, Opioids receptors and Eicosanoids signaling
Peptide hormones and growth factors signaling
Feedback and crosstalk signaling network
Cell Signaling and its Applications in Disease and Therapeutics
CELL BIOLOGY LAB PRACTICALS (01 CREDIT HOUR)
Cell and Tissue Culture
Membrane fractionation
Transient and Stable DNA Transfections
Tissue processing
Immunohistochemistry
Microscopy
Chromatin Immunoprecipitation
BOOKS RECOMMENDED:
Gene VIII By Lewin Benjamin Eds 2004. Oxford University press, Inc, New york.
Molecular Biology of the Gene by Watson, J. D., T. A. Baker, S. P. Bell, A. Gann, M. Levine, and
R. Losick, 5th Ed. 2003. New York, Benjamin Cummings ISBN 0-8053-4635-X
The Cell by Bruce Albert and Dennis Bray, 3rd Ed. Garland Publishing Inc, New York and London.
Biochemistry by Victor L. Davidson, Donald B. Sittman. 3rd Ed. 1993, Harwal Pub Co.
Cell and Molecular Biology by Gerald Karp. 1996, John Willey and Sons,Inc. London.
NCVI-813: GENOMICS AND PROTEOMICS (3 CREDIT HOURS)
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LEARNING OBJECTIVES: This course aims at offering an insight into genomics and proteomics
of biological systems, their diversity and various aspects as well.
COURSE CONTENTS:
Yeast genome as a model eukaryotic genome system
Genome diversity and evolutionary time-scale
Human Genome project, it implications on viral infections
Quantitative genetics
Linkage, crossing over and gene mapping in Prokaryotes
Linkage, crossing over gene mapping in Eukaryotes
Introduction to DNA microarrays
Expression profiling using microarrays
DNA microarray experimental design
DNA microarray data analysis and interpretation
Application of functional genomic research in the study of viruses
Proteome analysis
Techniques of proteomics
Proteomes in health and viral disease
Limitations and future development
BOOKS RECOMMENDED
Genomics, Proteomics and Bioinformatics by A. Malcolm Campbell and Laurie J. Heyer. 2002.
Benjamin Cumming Publishing Company.
Proteomics: From protein sequence to function by Pannington, S and M.J.Dunn. 2001. Spring
Verlag.
Introduction to Proteomics, Tools for the New Biology by Daniel C. Liebler. 2001. Humana
Press.
DNA Microarray: A molecular cloning manual by David Bowtell and Joseph Sambrook. 2003.
Cold Spring Harbor Laboratory Press, New York.
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MVI-820: MOLECULAR MICROBIOLOGY (3 CREDIT HOUR)
LEARNING OBJECTIVES: This is an introductory level course that describes various
biological and molecular aspects of bacteria. Our coverage will focus almost entirely on
bacteria that infect humans and cause serious disease.
COURSE CONTENTS:
The Bacterial Cell: An introduction to the structure of the bacterial cell.
Bacterial identification in the diagnostic laboratory versus taxonomy.
Taxonomic characterization of bacteria. Approaches to rapid diagnosis
Nutrition, Growth and Energy Metabolism: Anaerobic and aerobic metabolism. Metabolism
of sugars and fatty acids
Cell Envelope, spores and Macromolecular Biosynthesis: Structure and synthesis of the cell
walls of gram-positive and gram negative bacteria
Antibiotics - Cell Envelope: The mode of action of beta-lactate antibiotics.
Antibiotics - Protein Synthesis, Nucleic Acid Synthesis and Metabolism: The mode of action
of antibacterial chemotherapeutic agents. Antibiotic susceptibility testing.
Genetic Exchange: The mechanisms of gene transfer in bacteria; Insertion sequences,
transposable genetic elements and plasmids.
Genetic Regulatory Mechanisms: The structure and transcription of bacterial genes. The
molecular mechanisms that bacteria use to regulate gene activity. Inducible and repressible
operons. The molecular mechanisms involved in catabolite repression and attenuation. The
ways bacteria regulate enzyme activity.
Enterobacteriaceae: Enterobacteriaceae, Vibrio, Campylobacter and Helicobacter
Streptococci: Groups A, B and D streptococcus, pathogenesis, diagnosis. Streptococcus
pneumonia and Staphylococci. Streptococcus and pneumonia, Staphylococcus infections,
food poisoning, toxic shock Neisseria and Spirochetes: Syphilis, Lyme disease,
leptospirosis, gonorrhea, meningitis.
Anaerobes and Pseudomonas - Opportunistic Infections. Clostridia, gas-gangrene, tetanus,
botulism, pseudomonads.
Zoonoses: Listeria, Francisella, Brucella, Bacillus and Yersinia Plague, Anthrax,
Brucellosis, Listeriosis.
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General Aspects of Bacterial Pathogenesis: Exotoxins and endotoxins
LAB PRACTICALS
General Microbiological Laboratory Techniques
Media preparation
Identification of gram negative and positive strains
Growth curves for bacteria
Bacteriophage isolation
Bacterial conjugation for Plasmids transfer
Genetic complementation
Biosafety Guidelines
BOOKS RECOMMENDED
Medical Microbiology & Immunology by Warren Levinson & Ernest Jawetz. 7th Ed. 2003.
McGraw-Hill Publications. ISBN 0-07-122973-6.
Principles of Virology, Molecular Biology, Pathogenesis, and Control by S.J. Flint, L.W.
Enquist, R.M. Krug, V.R. Racaniello, and A.M. Skalka. 2nd Ed. 2000. ASM Press.
Fundamental Virology edited by D.M. Knipe and P.M Howley. 4th Ed. 2001, Lippincott Williams
and Wilkins.
MVI-830: BASIC IMMUNOLOGY (3 CREDIT HOURS)
LEARNING OBJECTIVES: This course will cover basic concepts of immunology and the
mechanism by which T and B lymphocytes acquire high level of specificity. Surface molecules will
be described in detail, followed by accessory and natural killer cells and human lymphocyte antigen.
Autoimmunity and tolerance will also be discussed.
COURSE CONTENTS:
The immune system
Origins of the immune system
Antibody structure and function and antibody diversity
Helper T cells and their activation
Organs of the lymphatic system
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T lymphocyte development and differentiation
B cell lymphocyte Development and Differentiation
Cell-cell interaction (T cells and Antigen presentation cells)
Non specific Defense Cells (Natural Killer cells)
Monocytes and Dendritic cells
HLA system (genomic organization, molecule structure, class I/II alleles and antigen
presentation).
The complement system
Innate immunity
Leukocyte migration
Pathological Immune mechanisms and tolerance
BOOKS RECOMMENDED
Introduction to Immunolgy by J.W. Kinhall, 1983, Macmillan Pub Co.
Immunology by Richard A.Goldsby, Thomas j.Kindt, Barbara A. Osborne, Janis Kuby, 2005. W.H
Freeman and Co.
Cellular and Molecular Immunology by Abbas, 2005. Elsevier Pub Co.
Color Atlas of Immunology by G.R. Burmester, A. Pezzutto, 2006. Thieme Stuttgart, New York.
MVI-831: LABORATORY APPLICATIONS OF IMMUNOLOGY ( 3 CREDIT HOURS)
COURSE OBJECTIVE: This course is designed to describe techniques for antibody production,
antigen detection, Immunofluorescence, and Immunohistochemistry.
COURSE CONTENTS
Antibody production (monoclonal and polyclonal)
Antigen-Antibody interaction
Definitions and precipitation techniques
Techniques of electrophoresis
Agglutination Techniques/complement binding Reaction
ELISA, RIA, and Immunoblotting
Immunrofluorescence, Immunohistology
Cellular Immunity
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Cell Isolation techniques
Test of T cell Functions
Antigen-specific Test
Assay procedure for characterizing antigen specific T cells
Humoral Immunity
Test for B-cell Function
Molecular Biological Methods
Analytical Techniques
MVI-832: IMMUNE DISORERS. (3 CREDIT HOURS)
LEARNING OBJECTIVES: Major human diseases attributed due to defects in immune system
will be discussed and immunological mechanism that governs the onset of key human diseases will
be eluded for their potential as therapeutic potential. Genes that contributes for these defects will
also be studied.
COURSE CONTENTS
Immunodeficiencies (Sever combined immunodeficiency syndrome)
Humoral and cellular Immunodeficiencies
Granulocytic Deficiencies
Complement Deficiencies and Defects
HIV Structure and Replication
Course of HIV Infection
Diagnosis and Treatment of HIV Infection
Hemolytic diseases and cytopenias
ABO blood group system Rhesus and other blood group systems
Mechanisms of hemolytic and antigen detection autoimmune diseases
Drug induced hemolysis
Hemolytic due to warm antibodies
Hematological Diseases
Acute Leukemias
Overview of Lymphoma Classification
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Hodgkin’s disease
T-Cell Lymphomas
B-Cell Lymphomas
Plasma Cell Dyscrasias
Multiple Myeloma
Cryoglulinemia
Amyloidsis
Tumor Immunology
Detection and identification of Tumor Antigens
Immune Escape Mechanisms of Tumor Antigens
Immunotherapeutic Strategies (I)
Immunotherapeutic Strategies (II)
Transplantation of Autologous
Bone Marrow/Heatopoietic Stem Cells
Transplantation of Allogenic bone Marrow/Hematopietic Stem Cells
Clinical Aspects of Organ Transplantation
MVI-833: CLINICAL IMMUNOLOGY 2 (3 CREDIT HOURS)
COURSE OBJECTIVE: Major human diseases attributed due to defects in immune system will be
discussed and immunological mechanism(s) that governs the onset of key human diseases will be
elucidated for their therapeutic potential.
COURSE CONTENTS
Musculoskeletal Diseases
Clinical features of Rheumatoid Arthritis
Synovial Changes in Rheumatoid Arthritis
Pathogenesis of Rheumatoid Arthritis
Juvenile Chronic Arthritis
Clinical features of Spondylarthritis
Pathogenesis of Spondylarthritis
Gout, Ploychondritis, and Bechet’s syndrome
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Skin Diseases
Urticaria
Contact Allergies
Atopic Dermatitis and Leukocytoclastic vasculities
Psoriasis and Bullous Skin Diseases
Gastrointestinal Diseases
Atrophic Gastritis, Whipple’s disease and Sprue
Chronic Inflammatory Bowl Diseases
Autoimmune Liver Diseases
Respiratory Diseases
Bronchial Asthma and Allergic Rhinitis Sarcoidosis and Idiopathic
Pulmonary Fibrosis
Extrinsic Allergic alveolitis Tuberculosis
Renal Diseases
Immunological mechanisms
Glomerulonephritis (I)
Glomerulonephritis (II) and Interstitial Nephritis
Metabolic Diseases
Autoimmune Thyroid Diseases
Diabetic Mellitus and Autoimmune Polyglandular Syndrome
Heart disease
Rheumatic fever, Myocarditis, and Postinfection syndrome
Neurolgical Diseases
Multiple Sclerosis
Autoantibody-mediated Diseases
Myasthenia Gravis and Lambert-Eaton syndrome
Ophthalmic Diseases
Anatomy and Pathogenesis
Extraocular inflammations
Uveitis (I)
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Uveitis (II) and Ocular Manifestation of Systemic Disease
Reproduction Immunology
Reproduction immunology
Gene therapy
Modern approaches and tools for gene therapy
JOURNALS RECOMMEDED
ANNUAL REVIEW OF IMMUNOLOGY, JOURNAL OF IMMUNOLOGY, EUROPEAN
JOURNAL OF IMMUNOLOGY, IMMUNOLOGY TODAY, ADVANCES IN IMMUNOLOGY,
CRC CRITICAL REVIEWS IN IMMUNOLOGY and HUMAN GENE THERAPY
MVI-840: PHARMOCOLOGY (Viral, Immune) (3 CRDIT HOURS)
Immune Pharmacology
(NSAIDS) and Glucocorticoids
Antimetabolites, Cyclophosphamide, Sulfasalazine, and Gold
Cyclosporin A, Mycophenolate, and Leflunomide
Nucleoside and non-nucleoside analogs
ATP-signaling and Viral response
Antiretroviral update
Xenobiotic Transport defects
Side effect of drugs and liver metabolism
Troxacitabine in acute leukemia
Varicella vaccine and fatal outcome in leukaemia
MVI-850: GENERAL GENETICS (3 CREDIT HOURS)
LEARNING OBJECTIVES: Medical genetics involves any application of genetics to medical
practice. The objectives of this course is to provide students with breadth and depth of knowledge to
make them realize the role of genetics in medicine and why the knowledge of medical genetics is
important for today’s health care facilitators and also for scientists and clinical researchers who are
interested in conducting research to elucidate the genetic basis of inherited disorders whose numbers
is increasing rapidly.
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COURSE DESCRIPTION:
This course includes detailed presentation of structure and function of genes, DNA analysis,
chromosomes and chromosomal aberrations; gametogenesis, Mendalian versus Non-Mendelian
inheritance; genomics and population genetics.
COURSE CONTENTS:
Medical Genetics in Perspective:
Developmental genetics-basic concepts
The human chromosomes
The life cycle of somatic cell
Meiosis
Human gametogenesis and fertilization
Medical relevance of mitosis and meiosis
The Human Genome: Structure and Function of Genes and Chromosomes:
DNA structure; A brief view
The central dogma: DNA RNA Protein
Fundamentals of gene expression
Structure of human chromosome
Variation in gene expression and its relevance in medicine
Techniques of Gene Analysis:
Analysis of individual DNA and RNA sequences
Methods of nucleic acid analysis
In Situ Hybridization to chromosomes
DNA sequence analysis
Methods of protein analysis
Pattern of Single-Gene Inheritance:
Terminology
Genetic disorders with Classical Mendelian Inheritance
Autosomal recessive inheritance
Pattern of autosomal dominant inheritance
X-linked inheritance
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Atypical pattern of inheritance
Genetic Variation in Populations:
Genetic diversity in human populations
Phenotypes, genotypes and gene frequencies
The Hardy-Weinberg law
Frequency of X-linked genes and genotypes
Factors that disturb Hardy-Weinberg equilibrium
DNA profiling- a practice test of Hardy-Weinberg assumptions
Gene Mapping and Human Genome Project:
Physical mapping of human genes
Mapping human genes by linkage analysis
Applications of human genome mapping
The human genome project
RECOMMENDED BOOKS:
Essential of Genetics by William Klug, Michael Cummings. Pearson Pretice Hall 2005.
Human Genetics by Ricki Lewis. McGraw Hill 2005
Genetics in Medicine: Robert Nussbauon, Roderide Mclnnes by Huntington Willard Thompson and
Thompson. Saunder 2004
Medical Genetics by Lynn Jorde, John, Michael Bamshad, Raymond White. Mobsy 2003.
Medical Genetics at a Glance by Dorian Pritchard, Bruce Korf. Blackwell S. Publications 2003.
MVI-851: MEDICAL GENETICS (3 CREDIT HOURS)
LEARNING OBJECTIVES: Emphasis is placed on the clinical application of Genetics. Such
topics include genetic assessment and counseling with prenatal diagnosis; chromosomal disorders;
single gene defects attributed due to viral integration; immunogenesis and cancer genetics;
multifactorial disorders congenital malfunctions; population screening; prevention and control;
treatment of genetic autoimmune immune diseases.
Clinical Cytogenetic:
Introduction to Cytogenetic
Chromosomal abnormalities
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Mendelian disorders with Cytogenetic effects
Cytogenetic analysis in cancer
Disorders of autosomes and sex chromosomes
Principles of Molecular Diseases:
Effect of mutation on protein function
Mutations disrupting the functions of biologically normal proteins
Hemoglobinopathies and HIV/HCV infection correlations
Molecular and Biochemical Basis of Genetics:
Diseases due to mutations in different classes of proteins
Enzyme Defects critical for viral infection
Defects in viral receptors proteins (chemokine receptors)
Disorders of structural proteins
Neurodegenerative disorders
Pharmacogenetic diseases
Genetics of Disorders with Complex Inheritance:
Genetic analysis of qualitative disease traits
Genetic analysis of quantitative traits
Genetic mapping of complex traits
Diseases with complex inheritance
Cancer Genetics:
Cancer as a genetic disorder
Characteristics of cancer cells
Genes that cause cancer-oncogenes; tumor suppressor p53, WLM, Rb, BRCC1
Genetic changes that cause some cancer-case studies
Cancer prevention, diagnosis and treatment
Diagnosis Strategies for Genetic Disorders:
Indication for prenatal diagnosis
Genetic counseling for prenatal diagnosis
Methods of prenatal diagnosis
Emerging technologies for prenatal diagnosis
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Laboratory investigations
Effect of prenatal diagnosis on the prevention and management of genetic diseases
Population screening (neonatal screening)
Overall impact of genetic disease
Types of genetic diseases
Current state of treatment of genetic diseases
Special consideration in treating genetic diseases
Treatment strategies
Genetic Counseling and Risk Assessment:
Genetic counseling
Case management in genetic counseling
Determining recurrence risks
Genetics and Society:
Population screening for genetic diseases
Ethical issues in medical genetics
Eugenic and dysgenic effects on gene techniques
RECOMMENDED BOOKS:
Genetics in Medicine by Robert Nussbauon, Roderide Mclnnes. Huntington Willard Thompson and
Thompson. Saunder 2004.
Essential of Genetics by William Klug, Michael Cummings. Pearson Pretice Hall 2005.
Human Genetics by Ricki Lewis. McGraw Hill 2005.
Medical Genetics by Lynn Jorde, John, Michael Bamshad, Raymond White. Mobsy 2003.
Medical Genetics at a Glance by Dorian Pritchard, Bruce Korf. Blackwell S.
MVI-852 IMMUNOGENETICS (3 CREDIT HOURS) INTRODUCTION
Genetics of AB and TCR genes
Mechanism of AB diversity
Somatic recombination
Somatic hypermutation
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B-cell receptor genes
T-cell receptor genes
RAG genes, Knockout mice
Inherited immune disorders.
Hyper Sensitivity
Autoimmunity
Immune Suppression.
MVI-860: VIRAL PATHOLOGY (3 CREDIT HOURS)
LEARNING OBJECTIVES: Major objective of this course will be to understand the viral
induced pathogenesis. The symptomatic of the disease and its progression into fatalities will be
discussed. The molecular pathways that lead to the induction of viral induced oral hairy leukoplakia,
insulin resistance, encephalitis, demyelination hemophagocytic Lymphohistiocytosis, choleostasis
hemorrhagic fever, hepatic fibrosis, cirrhosis and hepatocellular Carcinoma (HCC) will be studied.
COURSE CONTENTS
HCV -induced hepatic fibrosis
Viral induced fatty liver diseases (steatosis)
Hepatocellular carcinoma (HCC)
Viral induced arthrosclerosis
Viral induced ascitis
Viral induced insulin resistance
Viral meningitis and encephalitis
CMV retinitis
Falvi and foliovirus induced hemorrhagic fever
Antibody dependent enhancement (ADE) of Dengue Virus
Oncogenic viral pathology
Viral induced choleostasis
HIV/EBV induced Oral Hairy Leukoplakia (OHL)
Kaposi’s Sarcoma
Viral induced karatitis and conjunctivitis
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Viral induced myocarditis and inflammatory cardiomyopathy
Viral induced Emphysema
Virus induced autoimmune diseases
Virus Induced Demyelination
EBV-induced Hemophagocytic Lymphohistiocytosis
MVI-870: VACCINOLIOGY (3 CREDIT HOURS)
LEARNING OBJECTIVES: This course is deigned in such as way that students will be able to
design vaccines and their testing in yeast and mammalian cell, eggs and various animal models. The
history of vaccine, their efficacy, potential draw back/risks, impurities, cost effective, methods will
be discussed.
COURSE CONTENTS
History of vaccines
Viral proteins as potential targets for vaccine
Live versus attenuated vaccines
Role of multinational companies in vaccines production
DNA as vaccine
Peptide & Subunits vaccine
Adjuvants in vaccines
Population Genetic analysis: immunity to vaccine
Recombinant vaccine
Animal models of vaccine testing
Cost-effective approaches for production of new vaccines
Human testing and efficacy of vaccines: ethical issues
Recommendations of the Advisory Committee on Immunization Practices (ACIP)
Quadrivalent vaccine against human papillomavirus to prevent high-grade cervical lesions
Understanding the demand and supply of popular vaccine
Launching of vaccine and clinical trials
Anti-rabies immunoglobulin preparation based on F(ab')2 fragments
Effect of Panavir/Zanamivir on influenza A virus reproduction
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Vaccine safety
RECOMMENDED BOOKS:
Recombinant vectors in vaccine development by Fred Brown.
The Vaccine book by Barry R. Bloom.
Vaccine S by Plotkin_Orenstein.
Mass vaccine global aspect-progress and obstacles by Plotkin.
Pre-clinical and Clinical development of new vaccines by Fred Brown.
JOURNALS RECOMMENDED: VACCINE, JOURNAL OF ANTIMICROBIAL
CHEMOTHERAPY, CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, MEDICAL
MICROBIOLOGY AND IMMUNOLOGY
MVI-880: MOLECULAR TOXICOLOGY (3 CREDIT HOURS)
LEARNING OBJECTIVES: This course will provide a general understanding of the science of
toxicology as it relates to mankind and the environment at macro and molecular levels.
COUSE CONTENTS:
Fundamental concepts of Toxicology
Dose-response relationships
Effect of and viral infection and vaccines on liver toxicity
Absorption of toxicants, distribution and storage of toxicants
Biotransformation and elimination of toxicants.
Classes of toxins and their respective toxic effects on humans, animals and the environment.
The mechanisms of toxicity, describes medical, biochemical and genetic aspects of toxicology
including regulation of gene expression
Regulatory Toxicology, i.e., risk assessment and the chemo dynamics of viral contaminants in
the environment.
COURSE CONTENTS:
Part I: Basic Concepts
History and Basic Principles of Toxicology
Measurement of Toxicity: Toxicokinetics during viral infection
Absorption, Distribution, Storage, Elimination
Biotransformation: Metabolism and bioactivation of toxins
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Part II. Various classes of Toxicants:
Chemical Toxins
Toxicology of gaseous pollutants
Hydrocarbons
Halogenated hydrocarbons
Aromatic amines and N-nitroso compounds
Metals
Polymers
Cosmatics
Biochemical toxins
Insecticides and pesticides
Animal venoms and poisons
Plant toxins
Microbial toxins
Food and water borne toxins
Drugs and biopharmaceuticals
Part III. Mechanisms of Toxicity:
Organ System Toxicology
Hematotoxicity
Hepatotoxicity
Pulmonotoxicology
Nephrotoxicology
Neurotoxicology
Dermatotoxicology
Oto-oculartoxicology
Immunotoxicology
Genotoxicology
Carcinogenesis: DNA damage, DNA repair
Toxicogenomics: Induction and regulation of gene expression by toxins
Regulatory Toxicology:
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Toxicity assays
Risk Assessment
Controlling health hazards
Detoxification during and after viral infections
Bioremediation
Phytoremediation
BOOKS RECOMMENDED
Principles of toxicology by Williams and Roberts. 2nd Ed. A wiley Interscience publication.John
wiley and sons, Inc, New york
Toxicology by Hans Marquardt, Siegfried Schafer, Roger O. McClellan 1999. Academic Press Inc.
London.
Industrial Toxicology by Williams and Burson. 1989. John Willey and Sons.Inc.
Environmental Toxicology by Sethi, Iqbal, Satake, Mido. 2nd Ed.
Casarette & Doull’s Essentials of Toxicology by Curtis D.Klassen. John B. Watkins. 2003. Mc
Graw Hill.
Molecular Toxicology by P. David Josephy. Oxford University press.
Handbook of Toxicology by Michael Derelanko and Manfred Hollinger CRC Press 2004.
MVI-890: BIOTECHNOLOGY (3 CREDIT HOURS)
LEARNING OBJECTIVES: This course addresses the isolation, genetic manipulation, use of
organisms (commonly genetically modified) or their fermented food production, agriculture,
pharmaceutical discovery and production, molecular diagnosis, vaccine production, transgenic
animal formation, human gene therapy, forensic applications, microbial-base bioprocessing
pharming, bioterrorism and future of biotechnology. The course will consist of three parts:
COURSE CONTENTS:
Approaches of biotechnology
Specific applications
Social issues
I. APPROACHES OF BIOTECHNOLOGY:
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Structural Genomics:
Genome sequencing
Protein structure determination
Functional Genomics:
Bioinformatics
DNA microarray
Proteomics
ii) Modifying Protein Production and Function:
Protein engineering
Antisense technology
II. SPECIFIC APPLICATIONS:
Food Biotechnology:
Viral infection in food
Animals and microbes
Human Health and Diagnosis:
Viral diagnostics.
Forensic applications
Industrial Applications:
Bioprocessing
Microbial-based bioprocessing and Farming
Biosensors for detection of viruses
Recovering viral genomes and sequences
Environmental Applications of virus infection:
Bioremediation
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Waste management with virus contamination
Phytoremediation
Biofertilizers
SOCIAL ISSUES:
Rights and Privileges:
Intellectual property
Ethical implications
Risk and regulations
Perception and Fears about Viruses and Biotechnology:
Contrasting public and scientific perceptions of vaccines
Bioterrorism and bio defense
Biotechnology: The Future:
Purpose and expected outcomes
Technological advances in the pipeline
Third world participation in vaccine and biotechnology-bridging the gap
RECOMMENDED BOOKS:
Understanding Biotechnology: George Acquaah, Pearson Prentice Hall (2004).
MVI-900: BIOSTATISTICS (3 CREDIT HOURS)
LEARNING OBJECTIVES: This course is designed for students to understand different
computational biological algorithms, construct data models, use of molecular databases, use of
commonly available software for the analysis of bimolecular sequences and structures in viral and
cellular genes. They should be able to interpret the results, describe common post-genomic
experimental technologies associated with high-throughput data production.
COURSE CONTENTS:
Descriptive Biostatistics
Types of numerical data
Data sampling methods
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Graphical display of Data
Measurement of central tendency
Measurement of dispersion
Probability and probability distributions
Chi-Square Test
T-Test
Module II: Inferential Biostatistics
Sampling Distributions
Confidence Intervals
Baye’s Theorem
Hypothesis Testing
Experimental Design
Introduction to operating systems
Basics of Linux operating system
Words Processing, Spreadsheet and Presentation Skills
Internet for Biologists
Introduction to programming languages
Introduction to perl programming language
Programming skills in perl
Perl applications in bioinformatics
Introduction to databases
COBRA – Common Object Request Broker Architecture
MySQL database management system
MVI-901: BIOINFORMATICS ( 3 CREDIT HOURS)
Module I: Introduction to Bioinformatics
Definitions of bioinformatics and related fields
Objective and scope of bioinformatics
Kind of data used in bioinformatics
Data integration and data analysis
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Major biological databases
Module II: Sequence Alignment
Pairwise Sequence Alignment
Methods and tools of sequence alignment
Multiple Sequence Alignment
Module III: Phylogenetic Analysis
Phylogenetic trees
Methods of Phylogenetic analysis
Methods of evaluating phylogenies
Module IV: Gene Prediction
Introduction
Methods of gene prediction
Gene prediction tools
Module V: Protein Folding/Structure
Protein structure and its classification
Protein classification methodology and tools
Protein structure databases and visualization tools
Protein structure prediction
Protein function prediction
Module VI: Genomics and Proteomics
Introduction to genomics
Tools for genome analysis
Approaches for Genome-wide scan
Introduction to proteomics
Tools for proteome analysis
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LAB PRACTICLES:
Familiarization with Linux OS
Introduction to perl scripting
Creating a simple bioinformatics database
Accessing the publicly available databases
Pair wise and multiple sequence alignment
BLAST database and genome annotation
An Introduction to the vector/primer design Program
Protein structure prediction for known folds
Protein structure prediction for unknown folds
Visualization of biomolecular structures
BOOKS RECOMMENDED:
Fundamentals of Biostatistics by Bernard Rosner, Duxbury 2000.
An Introduction to Biostatistics by Glover & Mitchell, McGrawHill 2002.
Beginning of Perl for Bioinformatics by J Tisdall, O’Reilly 2002.
Bioinformatics: Sequence and Genome Analysis by DW. Mount, Clod Spring Harbor 2002.
Bioinformatics: Genes, Proteins & Computers by C.A. Orengo, Advanced Text 2003.
Bioinformatics: A practical guide to the analysis of genes and proteins by Baxevanis &
Ouellette, Wiley 2005.
TUTORIAL/ PRESENTATION (2 CREDIT HOURS)
SKILL ENHANCEMENT
LEARNING OBJECTIVES: This is a presentation skill enhancement course, which will enable
students to prepare seminar of assigned topics covering recent knowledge in the field. Evaluation
will be based on the quality of the review, oral presentation and comprehension of the subject. The
exact details of the activities will be described by the faculty accordingly.
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MVI-903: RESEARCH SEMINAR. (2 CREDIT HOURS)
PROPOSAL ASSIGNMENTS
LEARNING OBJECTIVES: NCVI will encourage working with clinicians and/other scientists to
develop translational overlaps so the field of investigation could be enhanced. This is a skill
enhancement course, which will enable students to participate in to variety of activities of their
choice. These will include either preparing a review in a given subject or preparing a research
proposal covering recent knowledge in that field. The exact details of the activities will be described
by the faculty time to time accordingly.
MVI-910: STEM CELL STUDIES (3-CREDIT HOURS) This course is designed to educate students about the new concepts of stem cell research. Primary
cell culture techniques, pluripotent cell isolation, dentriatic cells injection techniques will be taught.
COURSE CONTENTS
Hematopiotic stem cell/lineage
Bone marrow transplantation
Embryonic vs. Endodermal lineage.
Mesenchymal vs. Neural lineage
Proliferation markers
Cytokines & growth factor for stem cell culture
In vitro cell tracking (flowcytometry/FACS)
Chemical labeling of proteins and cells
Stable isotope labeling in culture medium
Bead based separation systems
Fluorescent and Colorimetric labeling & detection
HLA-typing
T-cell receptor gene transfer by lentiviral vectors in adoptive cell therapy
Karyotyping.
MVI-920 LAB DIAGNOSTICS/INSTRUMENTATION (3 Credit hours; TO BE OFFERED)
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MVI-930 BIOETHICS/BIOSAFETY (3 Credit hours)
LEARNING OBJECTIVES: This course will be offered into 2 parts. First part is designed to
educate students about work ethics, creating a culture for dedicated research, honesty/scientific
credibility, sexual harassment, clinical misconduct, patients confidently and plagiarism. Second part
will deal with safe handling of key chemicals, solvents, safe acids, base, liquid nitrogen fire, viruses,
bacteria and carcinogens. Proper Instrumental handling to protect oneself in case of accidents
MVI-940: EXPERIMENTAL THERAPEUTICS (3 Credit hours) LEARNING OBJECTIVES: This course is designed to search for the potential approaches that
will arrest the viral-induced pathogenesis and leukemia. The oxidative and anti oxidative signaling
pathways that are perturbed in many viral and immune diseases will be studied.
COURSE CONTENTS: Introduction
Experimental drug design
A virological benefit from an induction/maintenance strategy: the Forte trial
Interferon: cellular executioner
Therapeutic issues in HIV/HCV-coinfected patients
Tumor therapy mediated by lentiviral expression of shBcl-2 and S-TRAIL
Antihyperalgesic effect of a recombinant herpes virus
Silymarin (milk tussle) as potential therapeutics for oxidative stress
Flavinoid and Opinopid compounds as antiviral
Antioxidant therapies for cancer and viral infection (SAM, NAC)
Mucosal immunotherapy for alzheimer's disease with viral vectors
Genetic polymorphisms in the chemokine and chemokine receptors
Transition metal therapies during viral infection (zinc supplementation)
Role of cyclic nucleotide phosphodiesterase inhibitors as novel therapeutic agents for the
treatment of human lymphoid malignancies.
PDE4 inhibitors in primary human chronic lymphocytic leukemia (B-CLL)
AND-34, an SH2 domain-containing protein their role in viral diseases
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Focal adhesion protein p130Cas and role in human breast cancer cells
Hsp90, thioredoxin reductase, and other cytosolic translocation factors as therapeutics
Caspase and protease inhibitors to arrest viral replication
SiRNA/ShRNA and antisense approaches to arrest gene expression
BOOKS RECOMMENDED: (List to be provided later)
MVI-95: PLANT VIRUSES AND DISEASES (3 CREDIT HOURS)
Plant Viruses and their nomenclature
Sugar Cane Viruses
Woody plant virus
Cotton Curl Leaf virus
Other Cash crop viruses
Delivery of foreign genes into plants (Agro bacterium Ti based vs. shotgun approaches)
Plant viruses life cycle
Host interactions
Spread through insect vectors
Possible Antiviral approaches for Plant viruses
Viroids, Virosoids.
RECOMMENDED BOOKS:
Interaction between plant viruses and their vectors by R T Plumb.
Plant viruses and viral diseases volume XIII by Frans.
Identification of plant viruses methods and experiments by Nordun.
Atlas for insects and insects plant viruses by Karl.