Anetta Kuczyńska - Plant Phenotyping...Anetta Kuczyńska Institute of Plant Genetics, Polish...

Anetta Kuczyńska

Institute of Plant Genetics,

Polish Academy of Science,

Poznań, Poland

EPPN Plant Phenotyping Symposium, Barcelona 2015

INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY

EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND

POLAPGEN-BD: Biotechnological tools for breeding cereals

with increased resistance to drought

Task 2. Genetic maps and the location of QTLs associated with

barley resistance to the water deficiency

Project carried out under Innovative Economy Programme 2007-2013, Action 1.3,

Subaction 1.3.1. within the subject „Biological progress in agriculture and

environment protection”



EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND


THE AIM OF THE PROJECT

1. The general aim of the project was to enhance

competitiveness of breeding companies

producing cereal varieties and development of

biotechnological services in Poland. This is the

action that will lead to a biological progress.

2. Environmental protection.

3. Creating prototype biotechnological services,

which could be provided to breeders and

agricultural producers.


EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND

A system approach is achieved by adopting a model of tolerance to drought

stress containing ecophysiological, morphological, anatomical, metabolic,

proteomic, and molecular levels considered in the context of genetics.

TOPICS & PARTNERS



EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND

THE AIM OF THE STUDY

Mapping of quantitative trait loci (QTL) affecting yield and its

components in European/Syrian spring barley (Hordeum vulgare L.)

cross combinations under optimal and water shortage conditions.

genotyping

From

phenotyping QTL



EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND

PLANT MATERIAL

Three populations consisted of 100 RILs (F8) each, generated via SSD

technique in POLAPGEN project:

Maresi

(German, semidwarf

malting variety)

Cam/B1/CI 08887/CI

05761 (Syrian variety)

Maresi x Cam/B1/CI, Lubuski x Cam/B1/CI, Georgie x Harmal.



EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND

RIL POPULATIONS - OBTAINING

Fig. Steps in the procedure of obtaining RIL combined with in vitro culture: a) spikes and immature kernels from

which the embryos were dissected; b) embryos placed on B5 medium; c) embryos developed after 4 days of

culture; d) plantlets after 8 days of in vitro culture; e) plants transplanted in pots; f) spike plants after flowering stage



EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND

1. pF 2.2-3.0 – optimal conditions

GREENHOUSE EXPERIMENT

• the soil was taken from the Grabów Experimental Station (E 21º 39’,

N 51º 21’);

• the soil texture and properties were characterized by the Institute of

Agrophisics, Lublin;

• fertilization according to the Institute of Soil Science and Plant Cultivation,

Puławy;

• water retention curve pF;

The pF value is defined as a logarithm of the pressure p

(expressed in cm of water head) necessary for water removal

from soil capillaries.

– levels of soil moisture:

2. pF 3.2-3.6 – water deficit



EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND

EARLY DROUGHT (TREATMENT I)

Water shortage was imposed at the three leaf stage

(phase 13 in the BBCH scale) and maintained for 10 days.



EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND

LATE DROUGHT (TREATMENT II)

Water shortage was imposed at the flag leaf stage (phase 37 in the

BBCH scale) and maintained for 14 days.



EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND

MEASURMENTS OF SOIL MOISTURE

pF 3,2 3,4 3,6 3,8 4,2

Humidity %w/w

8,0 6,75 5,7 4,75 3,6



EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND

PHENOTYPING Traits

1. Grain weight per main spike (g)

2. Grain weight per lateral spike (g)

3. Grain weight per plant (g)

4. 1000-grain weight (g)

5. Length of main spike (cm)

6. Length of lateral spike (cm)

7. Number of grains per main spike

8. Number of grains per lateral spike

9. Number of spikelets per main spike

10. Number of spikelets per lateral spike

11. Fertility of main spike

12. Fertility of lateral spike

13. Length of main stem (cm)

14. Number of productive tillers per plant

15. Number of tillers per plant

16. Productivity of tillers

17. Straw weight per plant (g)

18. Heading stage (days)

Average

of 10 plants

Plants secured for biometric

observations



EUROPEAN UNION

EUROPEAN REGIONAL

DEVELOPMENT FUND

1H.2

8670-3880.0ABC09402-1-2-2751.23220-7232.1

6195-21373.74456-4435.0

3101-111 7372-12537.6

5494-31612.5Bmac021313.72609-35014.6

2901-113725.2

1906-42929.64226-570 5318-43630.1GBM121630.66792-194531.6Bmac006332.55194-111834.4

GBM1334 6081-85036.9

2265-36339.3596-103541.45381-195042.96382-396 409-16432314-1412 3277-446

43.3

5547-29447.011603-44548.010070-143548.87299-18348.9ABC16273-1-1-4849.5Bmac0032 1386-208850.76142-154452.04665-88252.26655-97853.4

2036-102757.1

1670-36961.0

1H_1

713-9720.0

Bmac01541.7

7083-7176.834-4727.22881-9357.56250-10569.0

9757-58212.7ABC08077-pHv131-0213.18867-459 GBM516213.71347-106114.1

ABC11290-sfp44-0618.0

13589-17520.9

4625-141327.25336-40027.3HVHVA127.53786-220427.8

4962-129531.35048-168532.6

557-1297 6547-136334.4

4978-103036.1

ConsensusGBS0554-4 ABC05061-1-1-1593671-59

43.3

9022-54343.7WMC1E844.0Bmag057944.6

4057-2114 13095-18747.7

1H_3

8670-3880.0ABC09402-1-2-2751.23220-7232.1

6195-21373.74456-4435.0

3101-111 7372-12537.6

5494-31612.5Bmac021313.72609-35014.6

2901-113725.2

1906-42929.64226-570 5318-43630.1GBM121630.66792-194531.6Bmac006332.55194-111834.4

GBM1334 6081-85036.9

2265-36339.3596-103541.45381-195042.96382-396 409-16432314-1412 3277-446

43.3

5547-29447.011603-44548.010070-143548.87299-18348.9ABC16273-1-1-4849.5Bmac0032 1386-208850.76142-154452.04665-88252.26655-97853.4

2036-102757.1

1670-36961.0

1H_1

713-9720.0

Bmac01541.7

7083-7176.834-4727.22881-9357.56250-10569.0

9757-58212.7ABC08077-pHv131-0213.18867-459 GBM516213.71347-106114.1

ABC11290-sfp44-0618.0

13589-17520.9

4625-141327.25336-40027.3HVHVA127.53786-220427.8

4962-129531.35048-168532.6

557-1297 6547-136334.4

4978-103036.1

ConsensusGBS0554-4 ABC05061-1-1-1593671-59

43.3

9022-54343.7WMC1E844.0Bmag057944.6

4057-2114 13095-18747.7

1H_3

3452-13550.09490-8430.8

HVM366.0

5880-254710.71865-39611.2GBM121411.67766-49212.48787-145912.77747-105612.9Bmag069216.0

1447-46426.2

3709-71628.4

6338-682 2964-382ABC03253-1-2-279

30.4

2651-177432.61341-84133.1

2417-92440.93122-90947.25379-595 ABC12560-1-1-42147.45113-62447.76384-866 GBM145947.92580-145648.4Bmac009351.25184-116353.24467-37453.3EBmac0684b54.9Bmag072055.77489-44256.210489-58658.87729-56560.02284-173860.34037-91662.8334-116463.52809-27164.5Bmag071164.84377-57165.54164-17665.98065-120366.85644-20670.07549-78270.5ABC09163-1-3-31372.26117-150772.5ConsensusGBS0705-180.13469-115281.87612-37582.78632-180983.46024-109583.5Bmag012584.16752-101384.82020-53989.75837-42793.1ABC10472-1-2-24793.6682-76793.85347-58593.911660-36595.6EBmatc003998.33206-67099.4ConsensusGBS0348-2100.0ABC13569-1-1-107100.8ABC04580-1-4-420101.42688-1022102.6111-499102.98501-449103.2GBM1309103.46996-838104.111979-928105.13180-1771106.714832-296108.6866-442108.95088-59109.0871-462109.27487-390109.4ABC01334-1-1-55109.5868-675109.74266-387111.5ABC09941-1-1-100 3000-1074113.2ConsensusGBS0379-1113.5EBmag0793114.2ABC01791-1-1-110 HVM54114.4GBM1469114.81250-923115.22464-1228 6328-736115.34472-658115.51613-291116.52822-739 285-2932117.53608-2133 6990-661121.27826-869122.23179-497122.43840-348122.5ABC10785-1-1-82122.66157-1233122.74241-445122.84240-749 252-556123.1GBM1462127.1

5419-760142.79910-427 6419-1680143.21449-1103 1344-930144.9

2H_6

1H.1 2H GBM12800.0

ConsensusGBS0194-14.61831-241 13924-4035.75533-3406.13344-11476.6GBM14506.77060-12376.95945-7487.61499-2908.83646-19848.97044-7059.41440-114812.44701-239516.02995-71717.2EBmac070519.43886-31320.75646-56822.23688-129124.84593-200729.91325-31130.84844-173731.16156-139932.12765-40632.36634-26335.76805-1110 3933-107738.04035-9938.34105-141738.7Bmag082842.1GBM144442.32838-66342.6Bmag013144.96491-29545.3ABC13089-1-2-47846.64728-173546.9ABC05866-1-1-31147.1ABC04375-1-1-17347.25657-1187 ABC05852-1-2-6586818-411

47.3

ConsensusGBS0697-1 3354-1212861-1941 ABC14167-1-2-250Bmag0138a 7716-7219658-234 10248-9543143-1276 2588-2294707-421

47.4

2288-126 6171-9565020-145 ConsensusGBS0471-11630-1150 GBM12137728-341

47.5

10353-119 2676-2564213-2378

47.6

ABC11783-1-1-99 2066-113347.7Bmag060347.8Bmac020947.9ABC10667-1-1-28848.23892-247249.65038-103552.02804-183252.5ABC08184-2-1-3554.1ABC18717-1-3-21555.9ABC14384-1-1-5356.36364-64556.74184-39357.28180-45057.72616-256057.9ABC19175-1-2-37558.81961-28760.25385-72260.35128-183160.5HVM3360.8GBM149561.4ABC13107-1-3-29961.810317-44861.91827-95862.42372-70362.5ABC10084-1-2-36363.21176-1547 2447-5461639-613

63.4

2677-50163.93470-13465.04618-155965.34150-39865.48722-51270.2HVM6075.5Bmag022577.4GBM109079.5GBM140583.6GBM123384.0Bmag0010b85.5ABC11028-1-1-6486.11898-580 6302-25087.33674-135288.13791-152588.9ABC18746-1-2-10990.86871-94595.27241-55395.71435-67095.87867-58596.61038-75497.3Bmag060698.83315-749101.22500-1514101.75488-1097103.4ABC07496-pHv1343-02103.99282-205104.476-1059104.86716-823105.33965-353105.45260-462105.84026-655107.82335-1614108.7ABC08260-1-1-108109.0ABC06381-1-5-73109.1Bmag0013116.8ABC05919-1-2-157 2847-48511657-398 9618-3722754-1027

123.5

EBmac0541124.9GBM1288132.1EBmag0705137.5

3H_8

ABC13678-1-2-3690.05334-15331.1

Bmag08773.45008-24023.64270-1844.2

7818-96711.0

EBmac070818.35840-65919.4ConsensusGBS0271-220.34787-174620.64737-36821.8

ABC38781-pHv2346-0128.04643-86728.87782-41029.6ConsensusGBS0632-329.9

265-122934.28752-52335.72346-31836.6GBM142037.4

scssr2553842.3

3H_5

3H.1

GBM12800.0

ConsensusGBS0194-14.61831-241 13924-4035.75533-3406.13344-11476.6GBM14506.77060-12376.95945-7487.61499-2908.83646-19848.97044-7059.41440-114812.44701-239516.02995-71717.2EBmac070519.43886-31320.75646-56822.23688-129124.84593-200729.91325-31130.84844-173731.16156-139932.12765-40632.36634-26335.76805-1110 3933-107738.04035-9938.34105-141738.7Bmag082842.1GBM144442.32838-66342.6Bmag013144.96491-29545.3ABC13089-1-2-47846.64728-173546.9ABC05866-1-1-31147.1ABC04375-1-1-17347.25657-1187 ABC05852-1-2-6586818-411

47.3

ConsensusGBS0697-1 3354-1212861-1941 ABC14167-1-2-250Bmag0138a 7716-7219658-234 10248-9543143-1276 2588-2294707-421

47.4

2288-126 6171-9565020-145 ConsensusGBS0471-11630-1150 GBM12137728-341

47.5

10353-119 2676-2564213-2378

47.6

ABC11783-1-1-99 2066-113347.7Bmag060347.8Bmac020947.9ABC10667-1-1-28848.23892-247249.65038-103552.02804-183252.5ABC08184-2-1-3554.1ABC18717-1-3-21555.9ABC14384-1-1-5356.36364-64556.74184-39357.28180-45057.72616-256057.9ABC19175-1-2-37558.81961-28760.25385-72260.35128-183160.5HVM3360.8GBM149561.4ABC13107-1-3-29961.810317-44861.91827-95862.42372-70362.5ABC10084-1-2-36363.21176-1547 2447-5461639-613

63.4

2677-50163.93470-13465.04618-155965.34150-39865.48722-51270.2HVM6075.5Bmag022577.4GBM109079.5GBM140583.6GBM123384.0Bmag0010b85.5ABC11028-1-1-6486.11898-580 6302-25087.33674-135288.13791-152588.9ABC18746-1-2-10990.86871-94595.27241-55395.71435-67095.87867-58596.61038-75497.3Bmag060698.83315-749101.22500-1514101.75488-1097103.4ABC07496-pHv1343-02103.99282-205104.476-1059104.86716-823105.33965-353105.45260-462105.84026-655107.82335-1614108.7ABC08260-1-1-108109.0ABC06381-1-5-73109.1Bmag0013116.8ABC05919-1-2-157 2847-48511657-398 9618-3722754-1027

123.5

EBmac0541124.9GBM1288132.1EBmag0705137.5

3H_8

ABC13678-1-2-3690.05334-15331.1

Bmag08773.45008-24023.64270-1844.2

7818-96711.0

EBmac070818.35840-65919.4ConsensusGBS0271-220.34787-174620.64737-36821.8

ABC38781-pHv2346-0128.04643-86728.87782-41029.6ConsensusGBS0632-329.9

265-122934.28752-52335.72346-31836.6GBM142037.4

scssr2553842.3

3H_5

3H.2

6133-7740.01996-6520.87385-7631.33026-10111.4ABC14522-1-8-3503.0GBM12214.23551-25376.52533-7737.42055-9479.0HVM4010.22065-313511.23432-29011.5GBM132317.11593-159717.62129-192818.21595-110718.86589-121121.9917-52223.02769-124523.38901-144623.814765-38830.41227-1323 10255-52930.75726-41434.93193-67135.3ABC09891-1-1-2835.55475-135536.0Bmac0303c36.56249-57236.96215-539 3190-6445298-828

37.1

4636-138037.2454-1502 3984-192037.4788-842 EBmac0906HVM03

37.7

1077-45738.0Bmac018138.37942-94839.5ABC10254-1-2-25040.01169-94440.32776-63741.8294-956 3078-219041.95889-1154 1304-47842.010346-9242.13716-91042.62274-122643.69208-91944.1Bmag049044.73127-27345.1GBM150946.75273-89447.7ABC09662-1-3-35247.910321-36449.14139-88849.9ABC09432-1-1-16052.26464-111552.411293-49053.93549-74355.1ABC14714-1-1-16256.3ConsensusGBS0589-156.9GBM129957.14407-134459.53416-69259.96172-773 EBmac077561.84807-132863.98463-46665.22196-19565.6ConsensusGBS0461-368.64564-60470.9ABC20090-1-1-27571.42400-95672.04039-168672.84986-121473.64133-60174.25245-30476.5ABC12093-1-2-23179.74535-136680.22574-41080.44361-186780.6EBmac0701 EBmac067981.4ConsensusGBS0288-185.541-69586.76519-81288.02614-152288.2WMS693.1ABC12417-1-1-4693.74160-136593.9ABC02813-1-4-326 4773-100994.14152-573 1954-119897.1HVM6797.32297-125098.21659-301 1010-126898.62878-574100.1954-1377101.1GBM1453101.2

4H_6

4H

ConsensusGBS0136-70.02057-4122.85148-14143.61872-13724.37185-3706.01769-545 2795-170712.61066-211013.9GBM127019.92188-42520.5885-10421.7GBM135522.45771-9124.03164-138625.412210-48028.61588-53730.06964-41432.73580-33134.95448-29836.22176-89138.53379-103740.04445-191140.24191-26841.21041-144141.4Bmag080741.66018-23541.83764-66641.9ABC04725-1-1-25442.2Bmag017345.06854-30947.62298-152647.94258-1498 ABC02895-1-4-23148.18220-1223 4313-48211016-603

48.6

4895-145 210-45049.51490-95949.73656-341 28-22949.81911-5551.0832-104051.1828-545 5873-88051.3397-28852.0851-80452.14721-133552.23658-131052.8ABC13045-1-1-22652.95017-407 2559-17963506-668

53.2

5232-204153.34656-25553.46487-131553.55497-66153.67282-138653.72259-48854.0Bmag000954.1HVM3154.2GBM501255.03348-39556.36205-68356.54203-121056.75554-197156.81565-51457.0ABC10738-1-1-25857.31140-150858.24064-172458.63048-134959.05187-75259.73773-75660.1Bmag061360.7EBmac080663.77361-937 EBmac06024454-1080

64.2

5172-92664.39251-85267.83987-1078 5965-138771.02968-1066 5926-79872.81969-132273.7578-58774.1428-151974.678-107375.0ABC12239-1-1-31675.78504-785 5684-60177.9ConsensusGBS0708-679.14374-60979.59981-66582.11473-111582.7ABC03519-1-3-13283.1ABC08038-1-3-16083.91597-15884.31007-65184.710535-21784.95225-70385.1GBM1087 ConsensusGBS0388-186.0GBM140486.45074-113586.93363-179598.2

6H_2

6H

1800-11010.0

Bmag01201.9

1178-2799.85796-118911.23492-67811.4

2378-49814.7

1894-213218.0

6628-130225.37397-85427.013008-35228.7EBmac075529.913108-41234.1382-262436.44137-79836.92966-62737.36433-12437.5277-93537.78758-56437.8ConsensusGBS0084-137.9ABC11252-1-2-25438.81590-54438.95595-29739.312368-20739.7Bmac015640.03579-70342.38923-70743.83818-112345.61437-68746.3Bmag013546.9

7H_16

1404-640.0

GBM13268.27634-6598.48365-4549.23313-14439.3452-4989.6EBmac06039.82124-984 8142-67610.25777-35410.3ABC03024-1-3-36812.06353-52415.01213-195916.02585-290119.8398-124420.1Bmag091420.9AF022725A23.5ConsensusGBS0356-124.2scssr07970a26.4GBM146426.7239-776 1792-3725467-1663 ABC10361-1-3-3168390-328

28.4

4679-159430.112239-66230.4943-310732.72669-101234.37810-113 5695-92235.6EBmatc001636.7HVCMA37.2497-38637.6ABC14535-1-1-7540.4Bmac018741.4ABC04803-1-1-39241.8

9820-45548.61107-39249.11518-62449.6

ABC09320-1-1-11258.0Bmac0167 ABC06618-1-2-3141997-77 EBmac08271290-729 5480-826

58.7

5138-26559.25-159359.34849-124862.2ABC05818-1-2-27162.82429-192964.1

5212-140975.1

11442-59286.7

7H_15

9728-10980.0GBM11261.73359-11181.8

2148-4983.6

Bmag020612.3

6394-944 1660-34713.8HVM04 6517-60214.9

1073-91621.5

6093-57225.03187-107325.8

7H_5

1800-11010.0

Bmag01201.9

1178-2799.85796-118911.23492-67811.4

2378-49814.7

1894-213218.0


7H_16

1404-640.0


28.4


9820-45548.61107-39249.11518-62449.6


58.7

5138-26559.25-159359.34849-124862.2ABC05818-1-2-27162.82429-192964.1

5212-140975.1

11442-59286.7

7H_15

9728-10980.0GBM11261.73359-11181.8

2148-4983.6

Bmag020612.3

6394-944 1660-34713.8HVM04 6517-60214.9

1073-91621.5

6093-57225.03187-107325.8

7H_5

1800-11010.0

Bmag01201.9

1178-2799.85796-118911.23492-67811.4

2378-49814.7

1894-213218.0


7H_16

1404-640.0


28.4


9820-45548.61107-39249.11518-62449.6


58.7

5138-26559.25-159359.34849-124862.2ABC05818-1-2-27162.82429-192964.1

5212-140975.1

11442-59286.7

7H_15

9728-10980.0GBM11261.73359-11181.8

2148-4983.6

Bmag020612.3

6394-944 1660-34713.8HVM04 6517-60214.9

1073-91621.5

6093-57225.03187-107325.8

7H_5

7H.1

7H.2

7H.3

9100-9780.04334-48210.63498-76114.14570-59114.3GBM122917.5Bmac016317.6ABC10705-1-1-26317.78118-100517.8ABC08327-1-1-353 152-32912005-188

17.9

4166-1090 8631-46918.17276-942 ConsensusGBS0527-52664-314 Bmac0306aBmac0113 4154-3643012-1125

18.2

5565-1908 8320-9554517-217 1286-9904392-450 5850-23478852-318

18.3

1215-862 8201-8618.49766-78718.5ConsensusGBS0654-418.62283-2913 7741-52718.76820-58618.94930-160419.012045-83 ABC09365-1-3-3787140-595 2146-2256

19.3

4565-1583 6367-45319.62505-85019.92482-12622.14234-194426.07839-633 2906-117726.47504-48526.9

5H_5

3417-14510.05206-7870.6166-1541.77310-9963.0scssr023064.4

10207-10248.1

847-139612.7

ABC01741-1-4-29915.32594-17216.44684-77517.1

3997-79618.8

5H_4

GBM14260.0scssr15334 GBM15064.83928-5135.79745-62810.6GBM148311.23685-89414.7Bmag022315.03333-120915.26833-65815.8370-44316.2Bmag081221.8956-47322.2ABC14689-1-9-39923.310318-57225.03398-163 4706-94026.810669-18827.36315-91428.5ABC15617-1-3-26528.82208-27929.56913-123530.73478-102431.4ABC11221-1-3-41036.3139-126343.93800-81444.23877-192344.512756-23344.7GBM500845.29566-1214 GBM143845.4ConsensusGBS0531-145.610047-33848.32352-27748.67167-46648.93985-82449.15844-101152.2211-25952.31164-268354.47639-12254.91697-63655.55571-64056.02091-63956.34795-78257.0ABC03900-1-2-406 7523-44058.41394-122258.6314-55959.05156-73759.56553-32860.2ConsensusGBS0138-261.5GMS06162.65286-48663.76050-162564.02520-718 2395-208364.33412-57964.7ABC04352-pHv108-0167.0447-8867.84346-84069.0407-259 1461-52970.0ConsensusGBS0086-571.51306-40872.03477-124872.92746-150173.5GMS02774.3EBmac082474.54050-2006 203-11288682-406

76.1

ConsensusGBS0397-16 1204-110476.57337-388 603-726054-1050

78.2

3759-138579.15126-1311 3443-1234Bmag0222

81.9

ConsensusGBS0451-182.9GBM149083.15925-56584.611470-47885.19864-22585.24015-115385.33720-52 5163-89685.47342-53585.62867-37385.93931-143487.03390-20588.3ABC07987-1-6-27088.65893-128589.4ConsensusGBS0269-190.45428-14690.93391-213793.2485-136993.62167-335 4066-32893.7ABC09278-1-4-6993.96735-75494.44845-123 4117-47294.5552-188 585-34297.16781-107397.93362-64498.14103-138698.32244-3247100.26761-490100.95145-1355101.62144-852102.110386-329102.35219-773103.04658-1237104.22493-660 6116-709105.16851-867105.22978-938108.1scssr03907109.512925-332116.0

5H_2

9100-9780.04334-48210.63498-76114.14570-59114.3GBM122917.5Bmac016317.6ABC10705-1-1-26317.78118-100517.8ABC08327-1-1-353 152-32912005-188

17.9


18.2

5565-1908 8320-9554517-217 1286-9904392-450 5850-23478852-318

18.3


19.3

4565-1583 6367-45319.62505-85019.92482-12622.14234-194426.07839-633 2906-117726.47504-48526.9

5H_5

3417-14510.05206-7870.6166-1541.77310-9963.0scssr023064.4

10207-10248.1

847-139612.7

ABC01741-1-4-29915.32594-17216.44684-77517.1

3997-79618.8

5H_4


76.1

ConsensusGBS0397-16 1204-110476.57337-388 603-726054-1050

78.2

3759-138579.15126-1311 3443-1234Bmag0222

81.9


5H_2

9100-9780.04334-48210.63498-76114.14570-59114.3GBM122917.5Bmac016317.6ABC10705-1-1-26317.78118-100517.8ABC08327-1-1-353 152-32912005-188

17.9


18.2

5565-1908 8320-9554517-217 1286-9904392-450 5850-23478852-318

18.3


19.3

4565-1583 6367-45319.62505-85019.92482-12622.14234-194426.07839-633 2906-117726.47504-48526.9

5H_5

3417-14510.05206-7870.6166-1541.77310-9963.0scssr023064.4

10207-10248.1

847-139612.7

ABC01741-1-4-29915.32594-17216.44684-77517.1

3997-79618.8

5H_4


76.1

ConsensusGBS0397-16 1204-110476.57337-388 603-726054-1050

78.2

3759-138579.15126-1311 3443-1234Bmag0222

81.9


5H_2

5H.3

5H.1

5H.2

1H 2H 3H 4H 5H 6H 7H

A consensus linkage map of barley constructed using three

RIL mapping populations and used for QTL analysis.

Mikołajczak K. et al. Quantitative trait loci for yield and yield-related traits in spring

barley populations derived from crosses between European and Syrian cultivars.

under review

EXPERIMENTS CARRIED OUT IN THE GREENHOUSE


Variation of the spike morphology


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EXPERIMENTS CARRIED OUT IN THE FIELD



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Setup experiment 1436PK

Phenotyping of 95 RIL barley lines plus 4 control varieties

8x replicates (2xcarrier per line; 4-pot config per carrier)

2x treatment conditions (control normal watering, stress conditions no watering)

Pre-cultivation phase: 4th of August – 3rd of September 2014

04.08.2014 Sowing of seeds in 96-well tray, soil mixture (Substrate 2 + Sand, 4:1)

24x seeds of each genotype distributed over 24x trays (1xreplicate/tray)

Greenhouse climate conditions:

16°C day/14°C night temperature

>65% relative humidity

Long day conditions (6.00-22.00) additional light

Continuous watering of trays as required (manually done by gardeners)

Transplanting of the plants in individual pots

20.08.2014 Transplanting of single plants in 3.5L pots for LemnaTec system

Soil mixture (Substrate 2 + Sand, 4:1)





Continuous watering of pots as required (manually done by gardeners)



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Phenotyping: 3rd of September – 8th October 2014

01.09.2014 Transfer pots from greenhouse to LemnaTec phenotyping facilities

28das (days after sowing)





Automatically watering based on watering regime (target weight)

Start watering 03.09.2014

Start imaging 04.09.2014

08.09.2014 Start stress (35das)

No/low watering to 20% field capacity

Normal watering 70% FC (control)

22.09.2014 Start recovery phase

Adding 300ml absolute watering to each pot

23.09.2014 additional target watering to reach 90% FC

25.09.2014 Fertilization, Combo Hakaphos blau (200ml absolute volume per plant)

01.10.2014 Fertilization, Combo Hakaphos blau (200ml absolute volume per plant)

Rotation of the carriers: each day for one lane, every 3rd day in between the lane for 11 positions



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All lines (RILs, varieties) (left) and varieties only (right)



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Post-cultivation: from 7th of October 2014

07.10.2015 Finish phenotyping, transfer of a selection of plants

(around 800 plants) for post-cultivation, harvest

Fig. Biometric analysis of spikes:

A – main spikes collected from a pot;

B – measurement of spikelets number per spike;

C – measurement of spike length



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Principal component biplot for traits observed after harvest; green – lines non-stressed, red – lines stressed

PC1 (36.34%)

PC

2 (

15.2

8%

)



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Institute of Plant Genetics Polish Academy of Science:

Maria Surma

Tadeusz Adamski

Paweł Krajewski

Karolina Krystkowiak

Krzysztof Mikołajczak

Piotr Ogrodowicz

Hanna Ćwiek

Leibniz Institute of Plant Genetics and Crop

Plant Research, OT Gatersleben, Germany:

Astrid Junker,

Kathleen Weigelt-Fischer,

Thomas Altmann



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Anetta Kuczyńska - Plant Phenotyping...Anetta Kuczyńska Institute of Plant Genetics, Polish...

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Transcript of Anetta Kuczyńska - Plant Phenotyping...Anetta Kuczyńska Institute of Plant Genetics, Polish...