An Introduction to the Histology of Lepidoptera Internal Anatomy... · •R.E. Chapman, The Insects...

96
An Introduction to the Histology of Lepidoptera Ray B. Nagle

Transcript of An Introduction to the Histology of Lepidoptera Internal Anatomy... · •R.E. Chapman, The Insects...

Page 1: An Introduction to the Histology of Lepidoptera Internal Anatomy... · •R.E. Chapman, The Insects Structure and ... •R.E. Snodgrass, Principles of Insect Morphology. Cornell Univ.

An Introduction to the

Histology of Lepidoptera

Ray B. Nagle

Page 2: An Introduction to the Histology of Lepidoptera Internal Anatomy... · •R.E. Chapman, The Insects Structure and ... •R.E. Snodgrass, Principles of Insect Morphology. Cornell Univ.

Histologic Preparation

• Tissue is fixed by cross-linking or

precipitating proteins.

• Tissue is dehydrated and embedded in

wax (paraffin).

• Thin (3-6um) sections are cut onto glass

slides using microtome.

• Sections are de-waxed, rehydrated, and

stained and coverslipped.

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Insect Histology

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Insect Histology Preparation

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The Integument

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Epidermis

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Intersegmental Cuticle

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Head Capsule vs Thoracic

integument

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Fig. 30

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Mechano receptors

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Trichogen and Gland Cells in

Larval Tubercle

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Fig. 29

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Lepidopteral Scales

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Fig. 32

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Simple Integumental Gland

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Epidermal Gland

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Fig. 26

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Apodeme

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Muscle attachment to Cuticle

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Fig. 10.5

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Molting (Apolysis) Epidermis in

G. incorrupta larvae

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Hardening the Pre-tanned

Exocuticle

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Male Reproduction

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Male Reproductive Tract

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Larval Testis

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Adult Testis

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Vas Deferens

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Seminal Vesicle

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Accessory Gland

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Female Reproduction

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Female Reproductive Tract

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Ovariole

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Maturing Ova

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Female Reproductive Tract

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Ostium Copulatrix and Vagina

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Arctiid Ova

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Fig. 12.14

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Crambidia cephalica

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H. Permaculata Accesory

Glands

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Female Accessory Glands in

Malacosoma

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Malacosoma ova covered with

spumaline

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Fig. 12.14

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Ostium copulatrix

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Bursa Copularix

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Spermatophore in Bursa Copulatrix

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Gastrointestinal System

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Fig. 190

Insect Gastrointestinal System

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Fig. 196

Larval GI Tract

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Fig. 191

Foregut (Stomodeum)

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Noctuid Larval Buccal Cavity

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Foregut-Midgut

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Proventriculus- Gastric Junction

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Gastrointestinal Tract

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Midgut

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Noctuid Larval Midgut

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Fig. 201

Midgut (Ventriculus)

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Peritrophic Membrane

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Fig. 202

Midgut Epiithelium

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Midgut in Larval Grammia

incorupta

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Midgut in Adult Arachnis picta

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Fig. 197

Changes in GI Tract with

Development

Larva

Pupa

Adult

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Gastrointestinal Tract

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Hindgut

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A. Picta Pylorus

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Midgut- Hindgut

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Colon and Rectum

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Fat Body in Larval Grammia

incorrupta

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Adult Fat Body

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Silk Gland

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Excretory System

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Malpighian Tubules Arising from

Midgut-Hindgut Transition

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Malpighian tubule

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Rectum

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Malpigian Tubules

Adherent to Hind Gut

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Nervous System

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Noctuid Larval Stemmata

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Compound Eye

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Adult Eye and Brain

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Ventral Nerve Cord

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Antennae

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Labial Palp

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Organ of vom Rath from Labial

Palp of Pieris rapae

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Respiratory System

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Larval Spiracles

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Noctuid Larval Tracheal System

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Tracheal System

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Circulatory System

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Heart

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Coelomocytes

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Melanization Reaction

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Parasite Encapsulation

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Acknowlegements

• William Meek

• Erika Dexter

• Edward Abril

• R.E. Chapman, The Insects Structure and

Function. Cambridge Univ. Press

• R.E. Snodgrass, Principles of Insect

Morphology. Cornell Univ. Press