An imagej plugin with cell tracking capabilities.
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Transcript of An imagej plugin with cell tracking capabilities.
![Page 1: An imagej plugin with cell tracking capabilities.](https://reader036.fdocuments.net/reader036/viewer/2022082816/56649d0c5503460f949df9a8/html5/thumbnails/1.jpg)
Manual Tracking
An imagej plugin with cell tracking capabilities
![Page 2: An imagej plugin with cell tracking capabilities.](https://reader036.fdocuments.net/reader036/viewer/2022082816/56649d0c5503460f949df9a8/html5/thumbnails/2.jpg)
Acquiring Manual Tracking The first step to acquiring the manual tracking
plugin is searching “imagej cell tracking” and clicking the first link.
• Once on the ibidi page select “Manual Tracking” from the left menu containing Product Categories
• From this page click the link shown below
![Page 3: An imagej plugin with cell tracking capabilities.](https://reader036.fdocuments.net/reader036/viewer/2022082816/56649d0c5503460f949df9a8/html5/thumbnails/3.jpg)
Acquiring Manual Tracking(cont.)
The next step is to download Manual Tracking from the purple link shown and move this file to the plugins folder of imagej
![Page 4: An imagej plugin with cell tracking capabilities.](https://reader036.fdocuments.net/reader036/viewer/2022082816/56649d0c5503460f949df9a8/html5/thumbnails/4.jpg)
Using Manual Tracking
Once in imagej, open the zvi file you wish to track.
Click the Plugins button and select Manual Tracking in the drop down menu
A new window should open. This window allows you to set the parameters for the track you are about to make.
The time interval and x/y calibration values can be selected here. A z value can also be calibrated if 3D tracking is being done.
![Page 5: An imagej plugin with cell tracking capabilities.](https://reader036.fdocuments.net/reader036/viewer/2022082816/56649d0c5503460f949df9a8/html5/thumbnails/5.jpg)
Using Manual Tracking
The Add track button in the top left should then be clicked.
Clicking on the nucleus will now produce xy points that pop up in a new window. If you continue to track the cell then the Manual Tracking program will produce distance and velocity values for the two points.
![Page 6: An imagej plugin with cell tracking capabilities.](https://reader036.fdocuments.net/reader036/viewer/2022082816/56649d0c5503460f949df9a8/html5/thumbnails/6.jpg)
Further Information
The two following links offer guidelines to doing other operations with the manual tracking plugin
http://rsb.info.nih.gov/ij/plugins/track/track.html http://
rsb.info.nih.gov/ij/plugins/track/Manual%20Tracking%20plugin.pdf