An Analysis of Amoxicillin Through GC/MS and Later FTIR Presentation
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Transcript of An Analysis of Amoxicillin Through GC/MS and Later FTIR Presentation
An Analysis of Amoxicillin Through GC/MS and Later FTIR
Establish the importance of the experiment◦ Handouts◦ Surveymonkey.com
62 participants
Survey Conducted
Primary Survey Questions
63%
37%
Question 1: Do you purchases pharmaceuticals in Mexico?
Yes No
14%
39%
18%
29%
Question 2: Why do you not purchase the products in Mexico?
Do Not Trust Them
Unsafe to Travel There
Too Far Away
Other
Primary Survey Questions - Continued
Pe-nacillin
31%
Amoxicillin31%
Erythromycin1%Lisinopril
1%
Valium3%
Vicodin3%
Ambien3%
Codeine4%
Demerol1%
Metformin4%
Steriod5%
Others13%
Question 5: What product(s) do you purchase in Mexico?
*selected all that applied
Primary Survey Questions - Continued
Yes63%
No30%
They Are Better8%
Question 11: Do you believe that they are of the same quality?
Cheaper69%No Prescription Is
Needed10%
I Live There5%
Other15%
Question 9: What is the primary reason you purchase Pharmaceuticals in Mexico?
Amoxicillin Samples to TestCVS
Pharmacy
GlaxoSmithKline
AMSA Laboratorios
Purchased from Medicinesmexico.com
GC/MS - AutoSampler
SampleSolvent
GC/MS – Gas Chromatography
GC/MS – Mass Spectrum
Now What?
Tetrahydrocannabinol - THC
C21H30O2
314.47 g/mol
A method to chemically modify a compound◦ Increase volatility◦ Decrease volatility
Focuses on O, S, N, and P functional groups with an active Hydrogen available
What is Chemical Derivatization?
Silylation produces silyl derivatives to increase volatility. ◦ Replaces active H with TMS.◦ Solvent = Pyridine
Acylation target carbohydrates and amino acids
Alkylation converts organic acids into esters by transferring an alkyl group
Three Types of Derivatization
Amoxicillin Structure
C16H19N3O5S = 365.4 grams/mol
MTBSTFA = C9H18F3NOSi (n-methyl-n-t-butyldimethylsilytrifluoroacetamide)
◦ 10,000 times more stable◦ Suitable for GC/MS analysis
Derivatization Reagent - MTBSTFA
C6H15Si
115.27 g/mol
C9H18F3NOSi
241.33 g/mol
+
MTBSTFA Reaction with Amoxicillin
0.0154 grams Amoxicillin 100 µL 0.85 M Acetonitrile 20 µL MTBSTFA Sat Overnight 150 µL Hexane *Centrifuged 15 Minutes 1 mL of Hexane (solvent)
Batch 1 Preparation – GC/MS
*Centrifuge Technique – GC/MS1. 2.
3. 4.
Batch 1 Run 1 Data – GC/MS
GC Parameters: Inlet – 240 ºC Oven temp. start at 60 ºC, Ramp up to 300 ºC at 40 ºC/min Flow rate of He = 1mL/min. Splitless
MS Parameters: Ion mode - 70 ev Solvent Delay 3 min. Scan – 50-500 amu
Batch 1 Run 1 Data – GC/MS
Batch 2 Preparation – GC/MSS1
0.1002 g of Amoxicillin 100 µL of Acetonitrile 1 mL of Pyridine (Solvent) 20 µL of MTBSTFA
S2 0.1012 g of Amoxicillin 100 µL of Acetonitrile 1 mL of Methanol
(Solvent) 20 µL of MTBSTFA
S1 & S2 *Centrifuged 20 min. Water bath 100 ºC 20
min.
Batch 2 Run 2, S1& Run 3, S2 Data – GC/MS
GC Parameters: Inlet – 250 ºC Oven temp. start at 40 ºC, Ramp up to 340 ºC at 20 ºC/min Flow rate of He = 1mL/min. Splitless
MS Parameters: Ion mode - 70 ev Solvent Delay 3 min. Scan – 50-500 amu
S1
S2
Batch 3 Preparation – GC/MS60 ºC
0.0983 g of Amoxicillin 100 µL of Acetonitrile 1 mL of Pyridine (Solvent) 20 µL of MTBSTFA *Centrifuged 20 minutes Water Bath 60 ºC 1 hour
40 ºC 0.1012 g of Amoxicillin 100 µL of Acetonitrile 1 mL of Methanol (Solvent) 20 µL of MTBSTFA *Centrifuged 20 minutes Water Bath 40 ºC 1 hour
Batch 3 Run 4, 40ºC & Run 5, 60 ºC Data – GC/MS
GC Parameters: Inlet – 250 ºC Oven temp. start at 40 ºC, Ramp up to 340 ºC at 20 ºC/min Flow rate of He = 1mL/min. Splitless
MS Parameters: Ion mode - 70 ev Solvent Delay 3 min. Scan – 50-600 amu
40 ºC
60 ºC
Batch 3 Run 5, 60 ºC Data – GC/MS
Batch 2 & 3 Data – GC/MS40 ºC
60 ºC
S1, 100 ºC
Water Bath Temp. Better Peaks
Batch 2 S1 Data – GC/MS
Batch 2 & 3 Data – GC/MS( +) 6-Aminopenicillanic Acid 4-Thia-1azabicycloheptane-2-carboxylic acid, 6-amino-3,3-dimethyl-
7-oxo(-) 6-Aminopenicillanic Acid
Handedness Chiral Molecule is a
molecule that is the same but arranged in a non-superimposable way, typically due to Carbon
C8H12N2O3S MW = 216.0568
H
Inconsistency Begins – GC/MS
What it should be
S1?, Run 6
GC Parameters: Inlet – 250 ºC Oven temp. start at 40 ºC, Ramp up to 340 ºC at 20 ºC/min Flow rate of He = 1mL/min. Splitless
MS Parameters: Ion mode - 70 ev Solvent Delay 3 min. Scan – 50-600 amu
Back to Working? – GC/MS
What it should be
S1, Run 19
GC Parameters: Inlet – 250 ºC Oven temp. start at 40 ºC, Ramp up to 340 ºC at 20 ºC/min Flow rate of He = 1mL/min. Splitless
MS Parameters: Ion mode - 70 ev Solvent Delay 3 min. Scan – 50-600 amu
Batch 4 – GC/MSStandard 1
0.1016 g of Amoxicillin 100 µL of Acetonitrile 1 mL of Pyridine (Solvent) 20 µL of MTBSTFA
Standard 2 0.0751 g of Amoxicillin 100 µL of Acetonitrile 1 mL of Pyridine (Solvent) 20 µL of MTBSTFA
Standard 3 0.0492 g of Amoxicillin 100 µL of Acetonitrile 1 mL of Pyridine (Solvent) 20 µL of MTBSTFA
Standard 4 0.0246 g of Amoxicillin 100 µL of Acetonitrile 1 mL of Pyridine (Solvent) 20 µL of MTBSTFA
Standards 1-4 *Centrifuged 20 min. Water bath 100 ºC 20 min.
GC Parameters: Inlet – 300 ºC Oven temp. start at 40 ºC, Ramp up to 340 ºC at 20 ºC/min Flow rate of He = 1mL/min. Splitless
MS Parameters: Ion mode - 70 ev Solvent Delay 3 min. SCAN - 50-600 amu
Batch 4 Sequence Run– GC/MS
GC Parameters: Inlet – 300 ºC Oven temp. start at 40 ºC, Ramp up to 340 ºC at 20 ºC/min Flow rate of He = 1mL/min. Splitless
MS Parameters: Ion mode - 70 ev Solvent Delay 3 min. SCAN – 50-600 amu
Standard 1Standard 2
Standard 3Standard 4
Batch 4 Sequence Run Calibration Curve – GC/MS
1000
020
000
3000
040
000
5000
060
000
7000
080
000
9000
0
1000
000
2000000
4000000
6000000
8000000
10000000
f(x) = 48.3028118594698 x + 3283814.61584145R² = 0.467955549500605
Graph 1: Concentration (PPM) Versus Area
Concentration (PPM)
Are
a
1000
020
000
3000
040
000
5000
060
000
7000
080
000
9000
0
1000
000
2000000
4000000
6000000
8000000f(x) = 48.3028118594699 x + 3283814.61584144R² = 0.52529673457978
Graph 2: Concentration Averages (PPM) Versus Area Averages
Concentration (PPM)
Are
a
Name Concentration (PPM) Area Average AreasStandard 4 21964 3124602Standard 4 21964 3059293Standard 4 21964 3087453Standard 3 43929 7201948Standard 3 43929 6591084Standard 3 43929 6425105Standard 2 67054 8825625Standard 2 67054 7083085Standard 2 67054 6681811Standard 1 90714 6788287Standard 1 90714 5668981Standard 1 90714 7278867
3090449.333
6739379
7530173.667
6578711.667
Table 1: Concentration Versus Areas
Batch 4, Standard 4 SIM Mode – GC/MS
S1, Run 30
GC Parameters: Inlet – 250 ºC Oven temp. start at 40 ºC, Ramp up to 340 ºC at 20 ºC/min Flow rate of He = 1mL/min. Splitless
MS Parameters: Ion mode - 70 ev Solvent Delay 3 min. SIM – 114, 142.5, 160
amu
SIM Mode focuses on specific m/z
Syringe filtered standards into new vials Ran sequence in SIM Mode - 160 m/z Results were inconclusive Build up suspected
Batch 4 Sequence 2 – GC/MS
Batch 5 – GC/MS Stock Solution 1026 PPM Amoxicillin Made 1000 PPB solution of Amoxicillin
Standard 1 2 mL of 1000 PPB Solution 0.5 mL of 5 N KOH
Standard 2 1 mL of 1000 PPB Solution 1 mL of DI Water 0.5 mL of 5 N KOH
Standard 3 0.5 mL of 1000 PPB Solution 1.5 mL of DI Water 0.5 mL of 5 N KOH
Standards 1-3 Water bath 50 ºC 20 min. Cooled for 60 Minutes + 1 mL of Acetic Acid + 5 mL of (7:1 ratio) Hexane: Ethyl Acetate solution Centrifuged for 15 minutes Top organic layer extracted to new test tubes Evaporated to dryness (several days) + 20 μL of MTBSTFA + 100 μL of Acetonitrile Heated in heating block at 110 °C for 15 minutes + 0.5 methanol solvent
Each standard tested several times No signs of Amoxicillin or 6-
Aminopenicillanic Acid
Batch 4 standard dilutions 100 μL of each respective standard into new
vial + 900 μL of Methanol solvent No signs of Amoxicillin or 6-Aminopenllinc
Acid
Batch 6 – GC/MS
Batch 7 – GC/MS
Test Samples 1 & 2 Water bath 60 ºC 15 min. Cooled for 60 minutes + 1 mL of Acetic Acid + 5 mL of (7:1 ratio) Hexane: Ethyl Acetate solution Centrifuged for 15 minutes Top organic layer extracted to new test tubes Evaporated to dryness (several days) + 20 μL of MTBSTFA + 100 μL of Acetonitrile Heated in heating block at 110 °C for 15 minutes + 0.5 methanol solvent
New Stock Solution of 10,000 PPM Amoxicillin Test Sample 1
2 mL of 1000 PPM Stock Solution 0.5 mL of 5 N KOH
Test Sample 2 2 mL of 10,000 PPM Stock Solution 0.5 mL of 5 N KOH
Each test sample tested several times No signs of Amoxicillin or 6-
Aminopenllinc Acid
Temperature affects the 8.10 peak of 6-Aminopenicillanic Acid
Amoxicillin as a whole cannot be seen in GC/MS
Conclusions from GC/MS Analysis
Amoxicillin - FTIRKBr + Amox
KBr + Starch
Standard Preparation - FTIRStandard 1
0.9916 grams of KBR 0.0017 grams of Amoxicillin 0.1026 grams of Starch
Standard 2 0.9970 grams of KBR 0.0034 grams of Amoxicillin 0.1003 grams of Starch
Standard 3 1.006 grams of KBR 0.0073 grams of Amoxicillin 0.1000 grams of Starch
Standard 1-3
Each standard tested 3 times
Starch Wavelength Height Ratios = 2150
Amoxicillin's Wavelength Height Ratios = 1775, 1687, 1686, 1685.5, 1519
Amoxicillin Standard Run Example - FTIR
2150
1775
16871686
1685.5
1519
Standard Data - FTIRStarch Abs. Ratio 1 Ratio 2 Ratio 3 Ratio 4 Ratio 5 Amox
1775 1687 1686 1685.5 1519 Height 2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150 Percentage1 1.0940 1.5688 1.5560 1.5855 1.4680 0.8225 1.3301 1.9074 1.8918 1.9277 1.7848 1.630%2 1.1990 1.5670 1.5550 1.5827 1.4540 0.8651 1.3860 1.8114 1.7975 1.8295 1.6807 1.630%3 1.1150 1.5706 1.5570 1.5881 1.4740 0.8749 1.2744 1.7952 1.7796 1.8152 1.6848 1.630%
Averages 1.1360 1.5688 1.5560 1.5854 1.4653 0.8542 1.3302 1.8380 1.8230 1.8574 1.7168 1.630%
Starch Abs. Ratio 1 Ratio 2 Ratio 3 Ratio 4 Ratio 5 Amox1775 1687 1686 1685.5 1519 Height 2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150 Percentage
1 1.2554 1.5946 1.6020 1.6060 1.4403 0.7923 1.5845 2.0126 2.0220 2.0270 1.8179 3.2787%2 1.2709 1.5905 1.5599 1.6060 1.4439 0.8025 1.5837 1.9819 1.9438 2.0012 1.7993 3.2787%3 1.2735 1.5934 1.6031 1.6084 1.4416 0.8105 1.5713 1.9659 1.9779 1.9845 1.7787 3.2787%
Averages 1.2666 1.5928 1.5883 1.6068 1.4419 0.8018 1.5798 1.9868 1.9812 2.0042 1.7986 3.2787%
Starch Abs. Ratio 1 Ratio 2 Ratio 3 Ratio 4 Ratio 5 Amox1775 1687 1686 1685.5 1519 Height 2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150 Percentage
1 1.7942 2.2060 2.2900 2.2400 1.8740 1.0290 1.7436 2.1438 2.2255 2.1769 1.8212 6.8034%2 1.7975 2.2030 2.2180 2.2260 1.8710 1.0395 1.7292 2.1193 2.1337 2.1414 1.7999 6.8034%3 1.7856 2.2005 2.2165 2.0140 1.8935 0.9207 1.9394 2.3900 2.4074 2.1875 2.0566 6.8034%
Averages 1.7924 2.2032 2.2415 2.1600 1.8795 0.9964 1.8041 2.2177 2.2555 2.1678 1.8926 6.8034%
Table 1: Standard 1 Data
Table 2: Standard 2 Data
Table 3: Standard 3 Data
RunAmoxicillin At Absorbance Height
RunAmoxicillin At Absorbance Height
RunAmoxicillin At Absorbance Height
1.0940/0.8225 = 1.3301(0.0017g Amox/ (0.0017g Amox + 0.1026 g Starch)) = 1.630%
Y Axis X Axis
Calibration Curves - FTIR
Sample 1 Ratio Interpretation - FTIR
Starch Abs. Ratio 1 Ratio 2 Ratio 3 Ratio 4 Ratio 51775 1687 1686 1685.5 1519 Height 2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150
1 - - - - - 0.5355 - - - - -2 - 3.9652 3.8270 3.7990 2.6780 0.5347 - 7.4157 7.1573 7.1049 5.00843 - 3.5608 3.5601 3.5599 2.6500 0.5342 - 6.6657 6.6644 6.6640 4.9607
Averages - 3.7630 3.6936 3.6795 2.6640 0.5348 - 7.0407 6.9108 6.8845 4.9846
Table 4: Average Ratios for Sample 1Amoxicillin At Absorbance Height
Run
Ratio 1 Ratio 2 Ratio 3 Ratio 4 Ratio 51775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150
1 - - - - - - - - - -2 - 7.4157 7.1573 7.1049 5.0084 - 78.7612% 66.0248% 91.9826% 101.5664%3 - 6.6657 6.6644 6.6640 4.9607 - 68.3662% 60.0643% 84.3602% 100.1124%
Averages - 7.0407 6.9108 6.8845 4.9846 - 73.5637% 63.0445% 88.1714% 100.8394%
RunPercentage Based Off of Calibration Curve With Respective Ratio
Table 5: Sample 1 Determined Average Percentage Based of Multiple Calibration Curves With Respective Height Ratios
Weight of Pill (g) 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150
1 - - - - - - - - - -2 - 78.7612% 66.0248% 91.9826% 101.5664% - 498.0068 417.4748 581.6061 642.20413 - 68.3662% 60.0643% 84.3602% 100.1124% - 432.2795 379.7866 533.4094 633.0106
Averages - 73.5637% 63.0445% 88.1714% 100.8394% - 465.1431 398.6307 557.5078 637.6074
- 46.4762 26.6496 34.0802 6.5008
- 465.14 ± 46.48 398.63 ± 26.65 557.51 ± 34.08 637.61 ± 6.50
(+) - 672.6441 517.6123 709.6647 666.6314
(-) - 257.6421 279.6491 405.3509 608.5833
Standard Deviation of Average Content of Amoxicillin (mg)
Concentration of Amoxicil lin (mg) and Standard Deviation
95% Confidence Interval of Amoxicillin (mg) ± Range
RunPercent Based Off of Calibration Curve With Respective Ratio
0.6323
Concentration (mg) Based Off of Wegiht of Pill and %Table 6: Sample 1 Determined Concentration (mg) of Amoxicil lin Determined from the Weight Pill (g) & Percentage
((7.4157 – 1.7325)/7.2158)) = 78.7612%0.6323 g X 78.7612% = 498.0068
3.9650/0.5347 = 7.4157
Sample 2 Ratio Interpretation - FTIR
Starch Abs. Ratio 1 Ratio 2 Ratio 3 Ratio 4 Ratio 51775 1687 1686 1685.5 1519 Height 2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150
1 2.5618 2.4935 2.5085 2.5160 1.9151 0.4569 5.6069 5.4574 5.4903 5.5067 4.19152 2.6050 2.4635 2.4862 2.4975 1.9185 0.4585 5.6816 5.3730 5.4225 5.4471 4.18433 2.5980 2.4507 2.4546 2.4560 1.9208 0.4579 5.6737 5.3520 5.3606 5.3636 4.1948
Averages 2.5883 2.4692 2.4831 2.4898 1.9181 0.4578 5.6541 5.3941 5.4244 5.4391 4.1902
Table 7: Average Ratios for Sample 2
RunAmoxicillin At Absorbance Height
Ratio 1 Ratio 2 Ratio 3 Ratio 4 Ratio 51775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150
1 5.6069 5.4574 5.4903 5.5067 4.1915 50.1877% 51.6219% 45.8671% 64.3538% 76.6796%2 5.6816 5.3730 5.4225 5.4471 4.1843 51.0439% 50.4512% 45.0473% 63.3241% 76.4599%3 5.6737 5.3520 5.3606 5.3636 4.1948 50.9540% 50.1613% 44.2987% 61.8808% 76.7800%
Averages 5.6541 5.3941 5.4244 5.4391 4.1902 50.7285% 50.7448% 45.0710% 63.1862% 76.6398%
Table 8: Sample 2 Determined Average Percentage Based of Multiple Calibration Curves With Respective Height Ratios
RunPercentage Based Off of Calibration Curve With Respective Ratio
Weight of Pill (g) 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150
1 50.1877% 51.6219% 45.8671% 64.3538% 76.6796% 289.6834 297.9614 264.7447 371.4502 442.59472 51.0439% 50.4512% 45.0473% 63.3241% 76.4599% 294.6255 291.2041 260.0129 365.5068 441.32663 50.9540% 50.1613% 44.2987% 61.8808% 76.7800% 294.1063 289.5313 255.6922 357.1758 443.1740
Averages 50.7285% 50.7448% 45.0710% 63.1862% 76.6398% 292.8051 292.8989 260.1499 364.7109 442.36512.7159 4.4633 4.5278 7.1704 0.9448
292.81 ± 2.72 292.90 ± 4.46 260.15 ± 4.53 364.71 ± 7.17 442.37 ± 0.94
(+) 297.3837 300.4235 267.7832 376.7993 443.9579
(-) 288.2265 285.3744 252.5166 352.6226 440.7722
Concentration of Amoxicill in (mg) and Standard Deviation
Concentration (mg) Based Off of Wegiht of Pill and %Table 9: Sample 2 Determined Concentration (mg) of Amoxicill in Determined from the Weight Pill (g) & Percentage
RunPercent Based Off of Calibration Curve With Respect ive Ratio
0.5772
95% Confidence Interval of Amoxicillin (mg) ± Range
Standard Deviation of Average Content of Amoxicillin (mg)
Sample 3 Ratio Interpretation - FTIR
Starch Abs. Ratio 1 Ratio 2 Ratio 3 Rat io 4 Rat io 51775 1687 1686 1685.5 1519 Height 2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150
1 4.0998 - - - 3.2080 0.7082 5.7890 - - - 4.52982 5.6195 3.4923 3.5105 3.5195 3.2560 0.7055 7.9653 4.9501 4.9759 4.9887 4.61523 3.7330 5.0030 5.1712 5.9810 3.2286 0.7037 5.3048 7.1096 7.3486 8.4994 4.5880
Averages 4.4841 4.2477 4.3409 4.7503 3.2309 0.7058 6.3530 6.0298 6.1622 6.7440 4.5777
Table 10: Average Ratios for Sample 3
RunAmoxicillin At Absorbance Height
Ratio 1 Ratio 2 Ratio 3 Ratio 4 Ratio 51775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150
1 5.7890 - - - 4.5298 52.2765% - - - 86.9853%2 7.9653 4.9501 4.9759 4.9887 4.6152 77.2359% 44.5911% 39.6474% 55.3989% 89.5862%3 5.3048 7.1096 7.3486 8.4994 4.5880 46.7229% 74.5179% 68.3380% 116.0883% 88.7596%
Averages 6.3530 6.0298 6.1622 6.7440 4.5777 58.7451% 59.5545% 53.9927% 85.7436% 88.4437%
Table 11: Sample 3 Determined Average Percentage Based of Multiple Calibration Curves With Respective Height Ratios
RunPercentage Based Off of Calibration Curve With Respective Ratio
Weight of Pill (g) 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150 1775:2150 1687:2150 1686:2150 1685.5:2150 1519:2150
1 52.2765% - - - 86.9853% 312.7182 - - - 520.34632 77.2359% 44.5911% 39.6474% 55.3989% 89.5862% 462.0250 266.7441 237.1710 331.3963 535.90463 46.7229% 74.5179% 68.3380% 116.0883% 88.7596% 279.4965 445.7662 408.7980 694.4403 530.9601
Averages 58.7451% 59.5545% 53.9927% 85.7436% 88.4437% 351.4132 356.2551 322.9845 512.9183 529.070397.2221 126.5877 121.3587 256.7109 7.9494
351.41 ± 97.22 356.26 ± 126.59 322.98 ± 121.36 512.92 ± 256.71 529.07 ± 7.95(+) 515.3164 921.4278 864.8111 1659.0482 542.4719(-) 187.5100 -208.9175 -218.8421 80.1389 515.6687
Concentration of Amoxicill in (mg) and Standard Deviation
95% Confidence Interval of Amoxicillin (mg) ± Range
0.5982
Standard Deviation of Average Content of Amoxicillin (mg)
Percent Based Off of Calibration Curve With Respect ive Ratio Concentration (mg) Based Off of Wegiht of Pill and %Table 12: Sample 3 Determined Concentration (mg) of Amoxicill in Determined from the Weight Pi ll (g) & Percentage
Run
Conclusions from FTIR Analysis
GlaxoSmithKline
AMSA Laboratorios
CVS Pharmacy
465.14mg ± 46.48mg292.81mg ± 2.72mg
Or442.37mg ± 0.94mg
529.07mg ± 7.95mg
Somewhat possible view Amoxicillin through GC/MS
Further testing of Amoxicillin Sample 2 through
Final Remarks
AMSA Laboratorios
THE END