AMS-01 STUBBLE TRIALS 2015

Independent herbicide

+ adjuvant trials

Replicated plots

By Agricultural

Academy

Institute of Agriculture

Karnobat, Bulgaria

Bulgaria Stubble Trials 2015

Independent trials carried out by KARNOBAT INSTITUTE

To test AMS-01 with Glyphosate 360 at various dose rates.

7 plots of replicated trials – annual weeds.

7 plots of replicated trials – perennial weeds.

Randomised complete block trial.

September treatment pre-planting of winter barley.

Trials site:

Agricultural Academy

Institute of Agriculture

Karnobat 8400

Bulgaria

Protocol

Location:

Field trials were carried in the experimental field of the Institute of Agriculture -

Karnobat. The field is located on leached, moderately heavy sandy clay to moderate

clay resinous. The soil profile reaches 100-110 cm, and the power of the humus layer

(A) ranges from 60 to 70 cm. The mechanical structure at the root layer is heavy sandy

clay. General humus content in the 0-60 cm horizon ranges from 2.48% to 2.86%.

The location of the experimental field of the Karnobat Institute of Agriculture is in the

northern part of South-Eastern Bulgaria. Climatically, the area belongs to the

transitional continental climatic zone with fairly long and cold spring, dry and hot

summers, prolonged and relatively moist autumn, relatively snowless winter with large

temperature variations and wind erosion.

Protocol

Weeds identified in the plots:

• Amaranthus retroflexus L.

• Amaranthus blitoides L.

• Chenopodium album L.

• Cirsium arvense (L.) Scop.

• Echinochloa crus-galli (L.) Beauv.

• Galinsoga parviflora Cav.

• Portulaca oleracea L.

• Setaria spp.

• Solanum nigrum L.

• Hordeum vulgare L.

Treatment Date: 25 September

Weather Conditions:

In the last ten days of September, average daily temperatures are in the range of 15.2 to 21.1°C.

On 24 September there fell 16.4 liters of rain per m2.

On 29 September - 14.0 l/m2.

Rainfall in October, l/m2:

06/10 - 8.6

09/10 - 5.1

10/10 - 9.5

11/10 - 5.8

12/10 - 2.3

13/10 - 3.2

Weather conditions were favourable for the growth and development of weeds.

Protocol

Randomised Block Design:

Total area of expertise (preparation, control, standard) all doses and repetitions - 700 m2

Area of treated fields (all fields that were treated with the compositions - one or more

repetitions fields): 1.25 x 10 = 12.5 m2 in quadruplicate.

Area of an option - 50 m2

Scheme experience - block method available options – randomised.

Products were applied in 200 L/ha of water.

Untreated Weeds – Actual Trials Site

Early kill of weeds is important for autumn and spring field work, to prepare for the following crop.

Speed of Activity

Product/Dose in L/Ha Weed Control -

5 days

Weed Control -

10 days

Weed Control -

21 days

Glyphosate 4.0 L/Ha 7.5% 50.0% 100%

Glyphosate 4.0 L/Ha

+ AMS 0.5 L/Ha10.0% 60.0% 100%

Speed of activity improved with AMS in critical early period.

Annual Weed Control

Speed of Activity

Annual Weed Control

AMS-01 increases speed of

activity of Glyphosate 360

Even at relatively high dose

of 4.0 L/ha

Gly

phosa

te

4.0

L/ha

7.5%

10%

20%

30%

40%

50%

60%

Gly

ph

osa

te 4

.0 L

/ha

+ A

MS

0.5

L/H

a

70%

80%

90%

10%

50% 60%

100%100%5 days

10 days

21 days

Gly

phosa

te

4.0

L/ha

Gly

phosa

te

4.0

L/ha

Gly

ph

osa

te 4

.0 L

/ha

+ A

MS

0.5

L/H

a

Gly

ph

osa

te 4

.0 L

/ha

+ A

MS

0.5

L/H

a

Speed of Activity

Product/Dose in L/Ha Weed Control -

7 days

Weed Control -

10 days

Weed Control -

21 days

Glyphosate 6.0 L/Ha 15 % 50 % 80 %

Glyphosate 6.0 L/Ha

+ AMS 0.5 L/Ha10 % * 55 % 90 %

Glyphosate 4.0 L/Ha

+ AMS 0.5 L/Ha20 % 45 % 100 %

Perennial Weed Control

* Anomalous Result

Speed of activity improved with AMS in critical early period.

AMS allowed reduced rate of Glyphosate – 30% saving.

Speed of Activity

Perennial Weed Control

Gly

phosa

te

6.0

L/ha

15%10%

20%

30%

40%

50%

60%

Gly

phosa

te 6

.0 L

/ha

+ A

MS 0

.5 L

/Ha

70%

80%

90%

10%

50% 55%

90%80%

7 days

10 days

21 days

Gly

phosa

te

4.0

L/ha

Gly

phosa

te

4.0

L/ha

Gly

phosa

te 4

.0 L

/ha

+ A

MS 0

.5 L

/Ha

Gly

phosa

te 4

.0 L

/ha

+ A

MS 0

.5 L

/Ha

20%G

lyphosa

te 4

.0L/ha

+ A

MS 0

.5 L

/Ha

45%

Gly

phosa

te 4

.0L/ha

+ A

MS 0

.5 L

/Ha

100%

AMS-01 increases

speed of activity

of Glyphosate 360

Reduced dose

+AMS gave 100%

after 21 days.

Comments

Heavy rain occurred 10 days after treatment by which time it appears all

products had been absorbed into the crop. No further assessments were

made until 21 days after application due to the weather conditions.

Comments

Comments from Associate Professor Dina Atanasova, Institute of Agriculture,

coordinator of the trials:

On Annual Weed Control:

On the 7th day of the treatment, in embodiments treated with a higher

dose and adjuvant AMS-01, symptoms of phytotoxicity were pronounced.

Our recommendations on the basis of observations from experience - after

10 days in areas infested with annual weeds, in co-treatment with

herbicides and adjuvant AMS-01, even in low doses, can be made

treatments to prepare the area for the next crops.

Comments

Comments from Associate Professor Dina Atanasova, Institute of Agriculture,

coordinator of the trials:

On Perennial Weed Control:

On the 7th day of the treatment there is already clear phytotoxicity in the form of

chlorosis and necrosis on the leaves, even at lower doses of herbicides. On the 10th

day of the treatment the symptoms cover almost the entire plant.

In visual observations of accounting for phytotoxicity, we had the impression that the

treatment with Glyphosate 360 SL together with adjuvant AMS-01 at a lower dose (400 ml/dka)

exhibits better efficacy compared to the application of Glyphosate 360 SL alone at higher

rates.

Our recommendations on the basis of observations from experience - after 21 days in co-

treatment with herbicides and adjuvant AMS-01, even in low doses, in areas infested with

perennial weeds can be made treatments to prepare the area for the next crops.

AMS-01 Visual Results

Glyphosate 4.0 L/Ha + AMS-01

10 days – Annual Weeds

Glyphosate 3.0 L/Ha + AMS-01

10 days – Annual Weeds

CONTROL PLOT

10 days – Annual Weeds

Cultivation is possible after

10 days.

AMS-01 Visual Results

Glyphosate 4.0 L/Ha + AMS-01

10 days – Perennial Weeds

Glyphosate 6.0 L/Ha + AMS-01

10 days – Perennial Weeds

Control Plot

10 days – Perennial Weeds

Glyphosate 6.0 L/Ha alone

10 days – Perennial Weeds

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