1-Filling Solutions sterilized by filtration are to be

filled under aseptic conditions. The containers and closures must be properly cleaned, sterilized and made available for use at this point in the process.

the product at this stage should be adequately protected so that it does not pick up any particulate or microbial contamination from the environment.

2-Sealing :

Sealing of the filled container should be done as soon as possible to prevent the contents from being contaminated. Sealing represents the final aseptic procedure. Ampoules are sealed by melting a portion of the glass neck with a fine jet of flame.

3-Sterilization Sterilization is process of complete

destruction or removal of all forms of life. Except in case of thermolabile substances, parenteral products are commonly sterilized after filling and sealing in the final containers and the process is called terminal sterilization.

Most useful sterilization method is Autoclaving which employs steam under pressure. It is probably the most effective method for sterilization of aqueous liquids or substances that can be reached or penetrated by steam.

Most useful sterilization method is Autoclaving which employs steam under pressure. It is probably the most effective method for sterilization of aqueous liquids or substances that can be reached or penetrated by steam.