ALTERNATIVES TO ALT - Diapharma · formalin-fixed paraffin-embedded or frozen tissue, flow...
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M30 AND M6 5 - NEXT GENERATION DILI MARKERS Ultimate Readout: Cell Death (Cyto)keratin 18 Safety Biomarkers for Drug Development For use in serum/plasma or cell culture applications DILI Avoid unwarranted progression of Phase 1 compounds destined to fail Prevent unwarranted discontinuation of drug development due to benign ALT elevations STOP GO M30 Apoptosense ® ELISA, M65 EpiDEATH ® ELISA, M65 ® ELISA, and M30 CytoDEATH™ are registered trademarks of VLVbio (Vivalavida AB). PEVIVA products are for research use only. See Pubmed for list of references. Necrosis Leakage of full-length K18 only intact K18 Nucleus K18 K18 M65 EpiDeath® ELISA Measurement of intact and cleaved K18 M65 EpiDeath® ELISA measures total cell death (necrosis and apoptosis) M6 M6 M5 M5 Apoptosis Disintegration of apoptotic bodies and release of K18 fragments Caspase cleavage of K18 caspase-cleaved K18 Asp396- Neoepitope Measurement of cleaved K18 only M30 Apoptosense® ELISA measures only apoptosis M30 Apoptosense® ELISA Y M5 Y M30 ALTERNATIVES TO ALT Is your research misled by your liver marker?
Transcript of ALTERNATIVES TO ALT - Diapharma · formalin-fixed paraffin-embedded or frozen tissue, flow...
M30 AND M65 - NEXT GENERATION DILI MARKERS
Ultimate Readout: Cell Death(Cyto)keratin 18 Safety Biomarkers for Drug Development
For use in serum/plasma or cell culture applications
DILI
Avoid unwarranted progression of Phase 1 compounds destined to fail
Prevent unwarranted discontinuation of drug development due to benign ALT elevations
STOP GO
M30 Apoptosense® ELISA, M65 EpiDEATH® ELISA, M65® ELISA, and M30 CytoDEATH™ are registered trademarks of VLVbio (Vivalavida AB).PEVIVA products are for research use only. See Pubmed for list of references.
Necrosis
Leakage offull-length K18
only intact K18
Nucleus
K18
K18
M65 EpiDeath® ELISA
Measurement of intact and cleaved K18
M65 EpiDeath® ELISA measures total cell death (necrosis and apoptosis)
M6 M6
M5 M5
ApoptosisDisintegration of
apoptotic bodies andrelease of K18 fragments
Caspase cleavageof K18
caspase-cleaved K18
Asp396-Neoepitope
Measurement of cleaved K18 only
M30 Apoptosense® ELISAmeasures only apoptosis
M30 Apoptosense® ELISA
YM5
YM30
A LT E R N AT I V E S TO A LTIs your research misled by your liver marker?
M 6 5 E p i D e a t h ® E L I S A ( R e s e a r c h u s e o n l y ) C A T # P 1 0 0 4 0
The M65 EpiDeath® ELISA is an updated version of the M65® ELISA and measures total CK18 in serum/plasma or cell culture samples. CK18 concentrations reflect total epithelial cell death (regardless of death mode).
M 3 0 A p o p t o s e n s e ® E L I S A ( R e s e a r c h u s e o n l y ) C A T # P 1 0 0 1 1
The M30 Apoptosense® ELISA is a unique biomarker assay that allows determination of epithelial cell apoptosis in blood samples. The assay is based on the unique M30 antibody which recognizes a neo-epitope of CK18 formed after caspase cleavage.
M30 Apoptosense® ELISA, M65 EpiDEATH® ELISA, M65® ELISA, and M30 CytoDEATH™ are registered trademarks of VLVbio (Vivalavida AB).PEVIVA products are for research use only. See Pubmed for list of references.
M 6 5 ® E L I S A ( R e s e a r c h u s e o n l y ) C A T # P 1 0 0 2 0
The M65® ELISA is the forerunner to the M65 EpiDeath® ELISA and is used to determine total epithelial cell death. Cell death mode (apoptosis or necrosis) can be determined by the combined use of the M65® ELISA and the M30 Apoptosense® ELISA. It is a biomarker for in vitro and in vivo studies using cell supernatant, serum or plasma samples.
M 3 0 C y t o D e a t h T M E L I S A ( R e s e a r c h u s e o n l y ) C A T # P 1 0 9 0 0
The M30 CytoDeath™ ELISA is intended for cell culture applications. Similar to the M30 Apoptosense® ELISA, this assay is based on the unique M30 antibody. The sensitivity and range of the assay have been optimized for in vitro applications (the assay is not suitable for serum/plasma samples). The M30 CytoDeath™ ELISA is a versatile tool to determine apoptosis in in vitro studies using monolayer cell cultures, multicellular spheroids or organ cultures. The ELISA is a powerful drug screening tool through its measurement of an accumulated-apoptosis product.
M 3 0 C y t o D e a t h T M A n t i b o d i e s ( R e s e a r c h u s e o n l y ) C A T # P 1 0 7 0 0 , P 1 0 7 5 0 , P 1 0 8 0 0 , a n d P 1 0 8 3 0
The M30 CytoDEATH™ antibody is used for the detection of epithelial cell apoptosis by immunohistochemistry in formalin-fixed paraffin-embedded or frozen tissue, flow cytometry or Western blotting. It detects caspase-cleaved human, monkey and bovine CK18 (but not the mouse, rat or canine protein). The M30 CytoDEATH™ mouse monoclonal antibody is available unlabeled, conjugated with biotin or fluorescein, or with orange dye.
M 5 a n d M 6 A n t i b o d i e s ( R e s e a r c h u s e o n l y ) C A T # P 1 0 6 0 0 , P 1 0 6 5 0 , P 1 0 8 0 0 , a n d P 1 0 8 3 0
The M5 and M6 Keratin 18 mouse monoclonal antibodies bind to conventional epitopes on Keratin 18 (CK18) between the caspase-cleavage sites aa238 and aa396. The antibodies are used for staining of epithelial cells by immunohistochemistry and for Western blotting. M5 Keratin 18 is specific for the human and monkey protein, whereas M6 Keratin 18 also binds to mouse, rat and dog CK18.
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M30 AND M65 - NEXT GENERATION DILI MARKERS
