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Transcript of Alternative activation of macrophages by IL-4 impairs phagocytosis of pathogens but potentiates...
![Page 1: Alternative activation of macrophages by IL-4 impairs phagocytosis of pathogens but potentiates microbial- induced signalling and cytokine secretion by.](https://reader036.fdocuments.net/reader036/viewer/2022062421/56649f3e5503460f94c5f281/html5/thumbnails/1.jpg)
Alternative activation of macrophages by IL-4 impairs phagocytosis of pathogens but potentiates microbial-
induced signalling and cytokine secretion
by Audrey Varin, Subhankar Mukhopadhyay, Georges Herbein, and Siamon Gordon
BloodVolume 115(2):353-362
January 14, 2010
©2010 by American Society of Hematology
![Page 2: Alternative activation of macrophages by IL-4 impairs phagocytosis of pathogens but potentiates microbial- induced signalling and cytokine secretion by.](https://reader036.fdocuments.net/reader036/viewer/2022062421/56649f3e5503460f94c5f281/html5/thumbnails/2.jpg)
Alternative activation of MΦs decreases N meningitidis uptake.
Audrey Varin et al. Blood 2010;115:353-362
©2010 by American Society of Hematology
![Page 3: Alternative activation of macrophages by IL-4 impairs phagocytosis of pathogens but potentiates microbial- induced signalling and cytokine secretion by.](https://reader036.fdocuments.net/reader036/viewer/2022062421/56649f3e5503460f94c5f281/html5/thumbnails/3.jpg)
IL-4 priming of MΦs does not modify SRA and MARCO expression or decrease N meningitidis binding at MΦ surface.
Audrey Varin et al. Blood 2010;115:353-362
©2010 by American Society of Hematology
![Page 4: Alternative activation of macrophages by IL-4 impairs phagocytosis of pathogens but potentiates microbial- induced signalling and cytokine secretion by.](https://reader036.fdocuments.net/reader036/viewer/2022062421/56649f3e5503460f94c5f281/html5/thumbnails/4.jpg)
Alternative activation potentiates N meningitidis–induced proinflammatory cytokine secretion via MyD88, independent of SRA and MARCO. (A) WT ThioMΦs were cultivated 48 hours in the
presence or absence of IL-4 and incubated for 24 hours with or without N men...
Audrey Varin et al. Blood 2010;115:353-362
©2010 by American Society of Hematology
![Page 5: Alternative activation of macrophages by IL-4 impairs phagocytosis of pathogens but potentiates microbial- induced signalling and cytokine secretion by.](https://reader036.fdocuments.net/reader036/viewer/2022062421/56649f3e5503460f94c5f281/html5/thumbnails/5.jpg)
IL-4 impairs uptake of RdGnX-N meningitidis and increases antibacterial proinflammatory response in vivo.
Audrey Varin et al. Blood 2010;115:353-362
©2010 by American Society of Hematology
![Page 6: Alternative activation of macrophages by IL-4 impairs phagocytosis of pathogens but potentiates microbial- induced signalling and cytokine secretion by.](https://reader036.fdocuments.net/reader036/viewer/2022062421/56649f3e5503460f94c5f281/html5/thumbnails/6.jpg)
IL-4 pretreatment and N meningitidis challenge inhibit Akt phosphorylation, but stimulate p42/p44 and p38 pathways.
Audrey Varin et al. Blood 2010;115:353-362
©2010 by American Society of Hematology
![Page 7: Alternative activation of macrophages by IL-4 impairs phagocytosis of pathogens but potentiates microbial- induced signalling and cytokine secretion by.](https://reader036.fdocuments.net/reader036/viewer/2022062421/56649f3e5503460f94c5f281/html5/thumbnails/7.jpg)
Microscopy analysis of zymosan phagocytosis by untreated and IL-4–treated ThioMΦs.
Audrey Varin et al. Blood 2010;115:353-362
©2010 by American Society of Hematology
![Page 8: Alternative activation of macrophages by IL-4 impairs phagocytosis of pathogens but potentiates microbial- induced signalling and cytokine secretion by.](https://reader036.fdocuments.net/reader036/viewer/2022062421/56649f3e5503460f94c5f281/html5/thumbnails/8.jpg)
Schematic summary of effect of IL-4 priming on phagocytic and secretory capacities of MΦs.
Audrey Varin et al. Blood 2010;115:353-362
©2010 by American Society of Hematology