Alison Van Eenennaam, Ph.D.Animal Biotechnology and Genomics Education Animal Biotechnology How,...

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Animal Biotechnology and Genomics Education Animal Biotechnology How, What and Why? Alison Van Eenennaam, Ph.D. Cooperative Extension Specialist Animal Biotechnology and Genomics [email protected] http://animalscience.ucdavis.edu/animalbiotech/ Vallejo 5/10/2006

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  • Animal Biotechnology and Genomics Education

    Animal Biotechnology How, What and Why?

    Alison Van Eenennaam, Ph.D. Cooperative Extension Specialist

    Animal Biotechnology and Genomics

    [email protected]

    http://animalscience.ucdavis.edu/animalbiotech/ Vallejo 5/10/2006

    mailto:[email protected]

  • Animal Biotechnology and Genomics Education

    What is Biotechnology ?

    Biotechnology - The application of science and engineering to living organisms.

  • Animal Biotechnology and Genomics Education

    Animal biotechnology

    Artificial selection (breeding programs)

    Artificial Insemination

    Embryo transfer

    Using DNA information for the marker-assisted selection of superior animals

    Cloning

    Genetic engineering

  • Animal Biotechnology and Genomics Education

  • Animal Biotechnology and Genomics Education

  • Animal Biotechnology and Genomics Education

    Manx cats Munchkin cats

  • Animal Biotechnology and Genomics Education

    Animal biotechnology

    Artificial selection (breeding programs)

    Artificial Insemination

    Embryo transfer

    Using DNA information for the marker-assisted selection of superior animals

    Cloning

    Genetic engineering

  • Animal Biotechnology and Genomics Education

    Cloning by embryo splitting – has been done for 20 years

  • Animal Biotechnology and Genomics Education

    Dolly the first adult cell cloned

    (1996)

  • Animal Biotechnology and Genomics Education

    What is DNA ?

  • Animal Biotechnology and Genomics Education

    Cloning by Nuclear Transfer at Cloning by Nuclear Transfer at

    Tissue biopsy is taken from donor cow, Daisy

    Donor cells are grown in tissue culture

    Donor cell nucleus is transferred to recipient egg

    Cloned embryo is transferred to surrogate mother

    Pregnancy is monitored by ultrasound

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    The two cloned calves,

    Dot and Ditto, on display at the State Fair,

    were born May 2003

    Cloning by Nuclear Transfer at Cloning by Nuclear Transfer at

    Tissue biopsy is taken from donor cow, Daisy

    Donor cells are grown in tissue culture

    Donor cell nucleus is transferred to recipient egg

    Cloned embryo is transferred to surrogate mother

    Pregnancy is monitored by ultrasound

    Em

    bry

    o T

    ran

    sfe

    r La

    bo

    rato

    ryE

    mb

    ryo

    Tra

    nsfe

    r La

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    rato

    ry UCUCDAVISDAVISCloning by Nuclear Transfer at Cloning by Nuclear Transfer at

    Tissue biopsy is taken from donor cow, Daisy

    Donor cells are grown in tissue cultureDonor cells are grown in tissue culture

    Donor cell nucleus is transferred to recipient egg

    Donor cell nucleus is transferred to recipient egg

    Cloned embryo is transferred to surrogate mother

    Cloned embryo is transferred to surrogate mother

    Pregnancy is monitored by ultrasoundPregnancy is monitored by ultrasoundPregnancy is monitored by ultrasound

    Em

    bry

    o T

    ran

    sfe

    r La

    bo

    rato

    ryE

    mb

    ryo

    Tra

    nsfe

    r La

    bo

    rato

    ry UCUCDAVISDAVIS

    The two cloned calves,

    Dot and Ditto, on display at the State Fair,

    were born May 2003

    Cloning by “Nuclear Transfer”

    (take nucleus and transfer it)

  • Animal Biotechnology and Genomics Education

    4/11/06

    Many animal species have been since been cloned from adult cells

  • Animal Biotechnology and Genomics Education

    Regancrest Emory Derry died unexpectedly.

    Who’s Buying? $20,000

  • Animal Biotechnology and Genomics Education

    •Full Flush Unable to supply market demand for his semen

    Who’s Buying?

  • Animal Biotechnology and Genomics Education

    The FDA continues to call for a voluntary prohibition of the marketing of milk or meat from

    SCNT clones and their offspring

  • Animal Biotechnology and Genomics Education

    Cloning used to multiply endangered species

    Bessie

    Surrogate Mother

    Noah

    Gaur Ox

    Who’s Buying?

  • Animal Biotechnology and Genomics Education

    Specialty Cattle Producers

    Starlight: record 77 inches ‘tip to tip’

    Who’s Buying?

  • Animal Biotechnology and Genomics Education

    Who’s Buying? http://www.savingsandclone.com/

    $50,000

  • Animal Biotechnology and Genomics Education

    Shift gears to Genetic Engineering

  • Animal Biotechnology and Genomics Education

    What is DNA ?

  • Animal Biotechnology and Genomics Education

  • Animal Biotechnology and Genomics Education

  • Animal Biotechnology and Genomics Education

    World Record 97 lb 4 oz. chinook salmon

    (Les Anderson, Kenai River, AK 1985)

  • Animal Biotechnology and Genomics Education 18 month old salmon

  • Animal Biotechnology and Genomics Education

    A B

    Genetically engineered animals for pharmaceutical production

    Cloned transgenic cows for human protein production

  • Animal Biotechnology and Genomics Education

    Nexia produces recombinant proteins in the milk of transgenic BELE® goats from which they are extracted. BioSteel™ filaments are used in applications where strength and

    flexibility are required, such as medical devices or body armor.

    http://www.nexiabiotech.com/

  • Animal Biotechnology and Genomics Education

    “Transplantation-friendly” miniature GE pigs….

    lack the allergenic proteins that cause the rejection of animal organs when used in

    human xenotransplantation surgeries

  • Animal Biotechnology and Genomics Education

    EnviropigTM (Low-phosphorus manure) http://www.uoguelph.ca/enviropig/

    Nature Biotechnology, 19, 741 - 745, (2001).

  • Animal Biotechnology and Genomics Education

    Animals, especially pets, occupy a special place in our society. The public is often unaware of animal agricultural practices.

  • Animal Biotechnology and Genomics Education

    Genetically engineered fish and cloned cats – oh my...

    12/2004

    http://www.glofish.com/default.asphttp://www.savingsandclone.com/index2.html

  • Animal Biotechnology and Genomics Education

    Interfering with the integrity or telos of an animal

    ‘will lead to abnormal outcomes’

    ‘man is playing God’

    ‘will cause economic disaster’

  • Animal Biotechnology and Genomics Education

    What’s up in the blogosphere ?

    Posted by Christina “Will cloned food sources by labeled as such? I am certainly not in support of it, will avoid it and will continue to support organic food sources as much as possible. What are our leaders thinking and why, as a US Citizen and registered voter, don't I get a say in this? Very disturbing!”

    Posted by Quignoff “There are many people that obviously do not know that we eat cloned, genetically engineered foods every single day. Nearly all of the plant foods that we eat have been engineered. Besides, our bodies have no idea that the food it's eating is cloned or not; dna is dna is dna as far as our stomachs and the microbes that live there are concerned.”

    Posted by Troy

    “Why not? Dead cow is dead cow regardless of its possible parentage.”

    http://www.supermarketguru.com/page.cfm/14894

  • Animal Biotechnology and Genomics Education

    Genetic

  • Animal Biotechnology and Genomics Education

    10

    20

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    50

    FIVE

    QUESTIONS

    GENETIC JEOPARDY

    40

  • Animal Biotechnology and Genomics Education

    ANSWER 1

    It is the application of

    science and engineering to

    living organisms

  • Animal Biotechnology and Genomics Education

    What is biotechnology ?

    QUESTION 1

  • Animal Biotechnology and Genomics Education

    ANSWER 2

    It is the dairy cow

  • Animal Biotechnology and Genomics Education

    QUESTION 2

    What is the black and white

    cow (Holstein breed) ?

  • Animal Biotechnology and Genomics Education

    ANSWER 3

    It is the process of making a

    genetically identical copy of

    an individual.

  • Animal Biotechnology and Genomics Education

    QUESTION 3

    What is cloning ?

  • Animal Biotechnology and Genomics Education

    ANSWER 4

    It is the name for all of the

    DNA in each cell of an

    organism.

  • Animal Biotechnology and Genomics Education

    QUESTION 4

    What is the genome ?

  • Animal Biotechnology and Genomics Education

    ANSWER 5

    She was the first animal

    cloned from an adult cell.

  • Animal Biotechnology and Genomics Education

    QUESTION 5

    Who was Dolly (the sheep) ?

  • Animal Biotechnology and Genomics Education

    FINAL JEOPARDY

    It is a linear molecule that is

    shaped like a double helix and it is made up of pairs of

    nucleotides

  • Animal Biotechnology and Genomics Education

    FINAL JEOPARDY

    What is DNA ?

  • Animal Biotechnology and Genomics Education