agriculture and environment testing - xema-medica.com reagents catalogue 2015.pdf · 3 ˜˚˛ 2015...

Reagents for food, agriculture

and environment testing 2015

1

ХЕМА 2015 Product Catalogue

Product List1.General information on reagents1.1. Monoclonal antibodies ..........................................................................................................................31.2. Antisera, neat ............................................................................................................................................31.3. Policlonal antibodies(gamma-globulin fraction of antiserum or avian amniotic fluid)31.4. Polyclonal antibodies (affinity purified) ..........................................................................................4

2. Antibodies for speciation for food control and forensic practice2.1. Serum albumins ......................................................................................................................................42.2. Haemoglobin ...........................................................................................................................................62.3. Uromodulin ..............................................................................................................................................72.4. Crustacean specific ................................................................................................................................82.5. Fish specific ..............................................................................................................................................82.6. Avian specific ..........................................................................................................................................82.7. Pork specific ..............................................................................................................................................82.8. Rodent specific ........................................................................................................................................8

3. Antibodies for determination of normal food constituents and allergens3.1. Prolamins .................................................................................................................................................113.2. Soy and other legumes ......................................................................................................................173.3. Vegetables, fruits and spices ........................................................................................................... 193.4. Nuts .......................................................................................................................................................... 213.5. Mushrooms ............................................................................................................................................ 263.6. Crustaceae ............................................................................................................................................. 273.7. Fish, amphibiae and lamprey .......................................................................................................... 283.8. Avian meats, eggs and other tissues ............................................................................................ 323.9. Mammal meats and other tissues ................................................................................................. 343.10. Milk ......................................................................................................................................................... 40

4. Antibodies against biologicals for agriculture and environmental control4.1. Ralstonia spp ......................................................................................................................................... 424.2. Fungal antigens ................................................................................................................................... 424.3. Artrhropodae ........................................................................................................................................44

5. Antibodies and conjugates for detection of LMW substances5.1. Antibiotics ...............................................................................................................................................465.2. Mycotoxins .............................................................................................................................................465.3. Plant steroids ......................................................................................................................................... 47

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http://www.xema-medica.come-mail: [email protected]

Dear Customers,

The Xema Corporate Group is one of the world’s leading biological companies providing antibodies and antigens for food, agriculture and environmental testing.Our major products include line of rapid tests for water quality, and detection of traces of human blood and urine, alcohol in beverage, pork meat and fat, and most important food allergens (fish, egg, milk, nut, soy, nuts). Please visit www.xematest.com to see more details of this product line. Xema also manufactures ELISA kits for laboratory use for detection of food constituents, allergens, contaminants, and control of environment.Please check our catalogue for short descriptions of our products you are interested. All reagents listed in this catalog are intended for in vitro research use only and not for in vivo applications. All reagents can be supplied in both small and large quantities according to the customers’ needs. Xema provides storage service for your bulk orders without additional charges. In addition, Xema produces a wide array of ready-made conjugates of our listed reagents for your direct applications. Xema also provides customers on-demand conjugation services for monoclonal and polyclonal antibodies to peroxidase, biotin, and various fluorescent dyes. Furthermore, we provide matched antibody pair information for ELISA, Western blotting, immunohistochemistry, and other biological applications.Please review this catalog or browse our website at www.xema-medica.com to find the reagent products that meet your needs. Moreover, if you have a collaborative project with Xema R&D department and agree for a joint publication, we will provide you all your required reagents without any charges. Please feel free to contact us for more details about our products and services. We will be happy to work with you, and answer any questions you may have.

Best regards

Contact info:Yuri LebedinGeneral ManagerXema group of [email protected] contact nearest Xema office (see www.xema-medica.com for details)

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1. General information on reagents

1.1. Monoclonal antibodies

Murine monoclonal antibodies are prepared from ascitic fluid as follows, if not stated otherwise

Purification methods Lon exchange or Protein A/G affinity (see actual Data Sheet)

Purity ≥95 % by SDS-PAGE

Concentration by OD280 0.5 - 10.0 mg/ml

Buffer 0.15 mM Tris + 0.15M NaCl + 0.05 % Na azide / 0.1M PBS + 0.05 % Na azide See actual Data Sheet

Storage Refrigerate at +2...+8 °C for no longer than 1 year, freeze for longer term

1.2. Antisera, neat

Purification methods High speed centrifugation and/or filtration

Purity Not applicable

Concentration by OD280 Not applicable

Buffer variants 0.05 % Na azide and.or 0.1 % BronidoxSee actual Data Sheet.

Storage Refrigerate at +2...+8 °C for no longer than 6 months, freeze for longer term

1.3. Polyclonal antibodies (gamma-globulin fraction of antiserum or avian amniotic fluid)

Purification methods Ammonium sulfate precipitation / Ion exchange (see actual Data Sheet)


Concentration by OD280 2.0-10.0 mg/ml

Buffer 0.1M PBS + 0.05 % Na azide and.or 0.1 % BronidoxSee actual Data Sheet


4


1.4. Polyclonal antibodies (affinity purified)

Purification methods Affinity purification on immunogen, adsorption on cross-reactive antigens (optional)


Concentration by OD280 0.5 - 10.0 mg/ml

Buffer 0.1M PBS + 0.05 % Na azide


2. Antibodies for speciation for food control and forensic practice

2.1. Antibodies to serum albuminsSpecies-specific antibodies to porcine serum albumin – see paragraph 3.9.

Murine monoclonal antibodies to serum albuminsSee table 2.1. below

Cat # Same as clone Id

Isotype IgG1

Immunogen Purified mammal albumins

Specificity All antibodies selected for high affinity for bovine or human serum albumin

Application Immunoassays, Western blotting

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Table 2.1. Monoclonal antibodies to serum albumins

Clone Id Human (HSA)

Cow (BSA) Swine Goat Sheep Horse Dog Cat Rabbit Rat

X01 + +++ +++ ++++ + + + ++ + +

X02 + +++ +++ ++++ + + + ++ ++ neg

X03 neg +++ ++++ ++++ +++ +++ + + ++++ neg

X04 ++++ +- ++++ +- neg +- +++ neg ++++ ND

X06 neg ++++ Neg +- neg +- +- ++ neg neg

X07 + +++ +++ +++ +++ ++ neg + neg neg

X12 ++++ ++++ ++++ ++++ ++++ neg ++++ ++++ ++++ ND

X13 +++ +++ +++ +++ + neg +++ +++ +- ++

X18 neg ++++ ++ ++++ +- neg neg +++ + ++++

X20 neg ++++ ++ neg +- neg neg neg neg neg

X40 neg +++ neg +++ +++ neg + +- neg neg

X418 ++++ ++++ ++ +++ ++ ND ++ ++ ND ND

Fig. 2.1. Monoclonal antibodies to serum albumins – blotting

9766

45

30

20

14

Сontrol X59 X12 X28 Ponceau

CS

A

HS

A

BS

A

BS

AH

SA

HS

A

HS

A

CS

A

BS

A

BS

A

BS

A

CS

A

MW, kD

SDS-PAGE 13% gel, w/o 2-MEBlotted mAbs (see clone Id’s on top) stained by anti-murine HRP and DAB chromogenAlbumins (see bottom):BSA – bovine; CSA – canine; HSA - human

6


Rabbit Antibodies to bovine serum albumin, affinity purified on antigen

Cat # AS280

Host Rabbit

Immunogen Purified bovine serum albumin

Specificity Cross-reactive to all mammal species


2.2. Antibodies to Human Haemoglobin (Hb)

Murine monoclonal antibodies to human haemoglobinSee table 2.2. below


Isotype IgG1

Immunogen Purified human haemoglobin

Specificity All antibodies selected for high affinity for human haemoglobin

Application Immunoassays for species-specific detection of human blood, Western blotting

Table 2.2. Murine monoclonal antibodies to human haemoglobin

Clone Id Isotype Species specificity* Pair Remark

X302 IgG1 human specific matched with X328

for human blood spot speciation

X305 IgG1 human specific matched with AS281


X317 IgG1 nonspecific

X328 IgG1 human specific matched with X302


*non-reactive to bovine, porcine, ovine, equine and avian Hb

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Fig. 2.2. Monoclonal antibody X328 to human haemoglobin- blotting

97

66

45

30

20

14

MW, kDM H HPl Pl

X328

SDS-PAGE 13 % gel, w/o 2-MEBlotted mAb stained by anti-murine HRP and DAB chromogenProteins (see bottom):PL – control antigen; H – human Hb

Rabbit Antibodies to bovine serum albumin, affinity purified on antigen

Cat # AS281

Host Rabbit

Immunogen Purified human haemoglobin


Application Immunoassays for species-specific detection of human blood (matched to mAb X305)

2.3. Antibodies to human uromodulin (Tamm-Horsfall protein, THP)

Murine monoclonal antibodies to human uromodulin

Cat # XT11, XT12, XT16

Isotype IgG1

Immunogen Purified human uromodulin

Specificity All antibodies selected for species specific binding of human uromodulin, nonreactive to canine, feline, bovine and equine THP

Application: Immunoass-ays for species-specific detection of human urine spots

8


Rabbit Antibodies to human uromodulin, affinity purified on antigen

Cat # AS287

Host Rabbit

Immunogen Purified human uromodulin


Application Immunoassays for species-specific detection of human urine spots (matched to mAb XT11, XT12, XT16)

2.4. Crustacean specific antibodies – see section 3.6

2.5. Fish specific antibodies – see section 3.7.

2.6. Avian specific antibodies – see section 3.8.

2.7. Porcine specific antibodies – see section 3.9.

2.8. Rodent specific antibodies

Rabbit Antibodies to heat-treated rat tissues, neat antiserum

Cat # AS369RC

Host Rabbit

Immunogen Heat-treated rat tissues (boiling for 30’)

Specificity Cross-reactive to mammal species

Application Immunoassays for rodent tissues

Rabbit Antibodies to heat-treated rat tissues, affinity purified on antigen

Cat # AS369RC A

Host Rabbit

Immunogen heat-treated rat tissues (boiling for 30’)



9


Rabbit Antibodies to raw rat tissues, neat antiserum

Cat # AS369RF

Host Rabbit

Immunogen Raw rat tissues



Rabbit Antibodies to raw rat tissues, affinity purified on antigen

Cat # AS369RF A

Host Rabbit

Immunogen Raw rat tissues



Canine Antibodies to heat-treated rat tissues, neat antiserum

Cat # cAS369RC

Host Dog




Porcine Antibodies to heat-treated rat tissues, neat antiserum

Cat # pAS369RC

Host Swine

Immunogen Heat-treated rat tissues (boiling for 30’



Porcine Antibodies to heat-treated rat tissues, affinity purified on antigen

Cat # pAS369RC A

Host Swine




10


Porcine Antibodies to heat-treated rat tissues, affinity purified on antigen, adsorbed on meat mix

Cat # pAS369RC AX

Host Swine


Specificity No reaction to mammal meats, weak reaction to chicken meat


Avian Antibodies to heat-treated rat tissues, affinity purified on antigen

Cat # yAS369RC A

Host Quail egg




Avian Antibodies to heat-treated rat tissues, affinity purified on antigen, adsorbed on meat mix

Cat # yAS369RC AX

Host Quail egg


Specificity Specific for rodent tissues


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3. Antibodies for determination of normal food constituents and allergens

3.1. Murine monoclonal antibodies to prolamins and other components of cerealsSee table 3.1. below


Isotype Various, see table

Immunogen Gliadin or hordein

Specificity See table for details and fig.3.1.1. for epitope map

Application Immunoassays for detection of gliadin, gliadin peptides, hordein or secalin; Western blotting

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Tabl

e 3.

1. M

onoc

lona

l ant

ibod

ies

agai

nst p

rola

min

s

All m

Abs s

trong

ly re

act w

ith gl

iadin

(++

+)

SPEC

IES RE

ACTIV

ITY

DGP f

usion

****

Clone Id

Isotype

Specificity by im-munoblotting

Rye (Secalin)

Monococcum

Oat (Avenin)

Barley (Hordein)

Sesame

Corn (Zein)

Heat resistance**

Water soluble***

rGliadin omega

DGP1

DGP2

DGP4

Bdg of Gliadin-biotin

R5* inhibition

Remark

Suggested epitope group

XGY1

IgG1

alpha

, bet

a, ga

mm

a, om

ega

++

++

selec

ted*

++

neg

neg

mod

erat

ene

gne

gne

gne

gne

gne

gno

pan-

prola

min,

PO

LYM

ERIC

epito

peI’

XGY2

IgG2

aalp

ha, b

eta,

gam

ma

neg

++

+-

+ne

gne

gN/

DN/

Dne

gne

gne

gne

gN/

Dno

gliad

in sp

ecifi

c, no

t su

itable

fo

r HRP

O lab

elling

by

perio

date

IV

XGY3

IgG1

non r

eac-

tive

neg

++

neg

selec

ted*

neg

neg

no+

++

neg

3+3+

3+1+

no

gliad

in sp

e-cifi

c, pr

efers

aesti

vum

wh

eat

V

XGY4

IgG1

gam

ma,

omeg

a+

++

+se

lecte

d*+

++

neg

good

+ne

g4+

4+4+

neg

noDG

P blot

ting

nega

tive

V

XGY5

IgG1

non r

eac-

tive

neg

++

neg

neg

neg

neg

mod

erat

e+

++

neg

+-

+-

+-

1+no

gliad

in sp

ecifi

cI a

13


Tabl

e 3.

1. M

onoc

lona

l ant

ibod

ies

agai

nst p

rola

min

s (c

onti

nued

)

Clone Id

Isotype


Rye (Secalin)

Monococcum

Oat (Avenin)

Barley (Hordein)

Sesame

Corn (Zein)

Heat resistance**

Water soluble***

rGliadin omega

DGP1

DGP2

DGP4


R5* inhibition

Remark

suggested epitope group

XGY6

IgG1

omeg

a, ga

mm

a+

++

+se

lecte

d*+

++

-ne

gex

celle

nt+

++

+ne

g2+

4+ne

gno

POLY

MER

IC ep

itope

II’

XGY7

IgG1

(bet

a),

gam

ma,

omeg

a+

++

neg

++

neg

neg

low+

++

neg

neg

neg

neg

1+no

I a

XGY8

IgG1

beta

, alp

ha?

++

neg

++

neg

neg

lowne

gne

gne

gne

gne

g2+

noIII

XGY9

IgG1

beta

, ga

mm

a+

++

+ne

g+

+ne

gne

glow

++

+ne

gne

gne

gne

g1+

noI a

XGY1

0Ig

G1ga

mm

a (o

meg

a)+

++

selec

ted*

++

neg

neg

good

+ne

g3+

3+3+

neg

noII

XGY1

1Ig

G1alp

ha, b

eta,

gam

ma

neg

++

neg

neg

+we

akm

oder

ate

neg

neg

+-

+-

+-

3+no

gliad

in sp

ecifi

c, DG

P blo

tting

+

++

IV

XGY1

2Ig

G1alp

ha, b

eta,

gam

ma,

omeg

ane

g+

+ne

gse

lecte

d*ne

gne

gm

oder

ate

++

neg

+-

+-

+-

3+no

IV

XGY1

3Ig

M(g

amm

a),

omeg

a+

++

weak

weak

neg

+m

oder

ate

neg

+ne

gne

gne

gne

gno

reco

gnize

s ze

insp

ecial

14


Tabl

e 3.

1. M

onoc

lona

l ant

ibod

ies

agai

nst p

rola

min

s (c

onti

nued

)

Clone Id

Isotype


Rye (Secalin)

Monococcum

Oat (Avenin)

Barley (Hordein)

Sesame

Corn (Zein)

Heat resistance**

Water soluble***

rGliadin omega

DGP1

DGP2

DGP4


R5* inhibition

Remark

Suggested epitope group

XGY1

5Ig

G1om

ega

neg

++

neg

++

neg

neg

low+

++

neg

neg

neg

neg

neg

noII

XGY1

6Ig

G1ga

mm

a, om

ega

neg

++

selec

ted*

++

neg

neg

no+

+ne

gne

gne

gne

gne

gno

III

XGY1

7Ig

G1ga

mm

a, (o

meg

a)ne

g+

+ne

g+

neg

neg

no+

++

-+

-+

-+

-ne

gno

I a

XGY1

8Ig

G1be

ta,

gam

ma

++

+se

lecte

d*+

+ne

gne

gno

neg

neg

neg

neg

1+1+

noI b

XGY1

9Ig

G1be

ta,

gam

ma,

(om

ega)

++

++

weak

++

neg

weak

no+

+ne

gne

gne

g+

-1+

+-

I b

XGY2

0Ig

G1be

ta,

omeg

a W

EAK

++

neg

neg

selec

ted*

neg

weak

good

neg

neg

neg

neg

neg

neg

no

Non r

eacti

ve

again

st DU

-RU

M w

heat

; co

nfor

mat

ion

epito

pe;

used

in K3

81

Duru

m EI

A

spec

ial

15


Tabl

e 3.

1. M

onoc

lona

l ant

ibod

ies

agai

nst p

rola

min

s (c

onti

nued

)

Clone Id

Isotype


Rye (Secalin)

Monococcum

Oat (Avenin)

Barley (Hordein)

Sesame

Corn (Zein)

Heat resistance**

Water soluble***

rGliadin omega

DGP1

DGP2

DGP4


R5* inhibition

Remark

suggested epitope group

XGY2

2Ig

G1N/

Dne

g+

+ne

gne

gne

gne

ggo

od+

+ne

gne

gne

gne

g1+

nogli

adin

spec

ific

XGY2

3Ig

G1N/

D+

++

neg

++

++

neg

low+

++

1+ne

gne

gne

g3+

noII

XGY2

4Ig

G1N/

Dne

g+

+ne

g+

++

neg

good

++

+3+

neg

neg

neg

4+2+

II

XGY2

5Ig

G1N/

D+

++

++

++

+ne

gne

gex

celle

ntne

g3+

neg

neg

3+N/

D1+

pan-

prola

min

I b

XGY2

6Ig

G1N/

D+

++

++

++

+ne

gne

glow

neg

3+ne

gne

g3+

N/D

nopa

n-pr

olam

inI b

N/D

not d

eter

mine

d

*se

lecte

dva

riable

with

in sp

ecies

(sor

t dep

ende

nt)

**he

at

resis

tanc

ehe

ating

to 10

0 °C,

30’

***

wate

r so

luble

disso

lved i

n 0.1M

PBS a

nd cl

eare

d by h

igh sp

eed c

entri

fuga

tion

****

DGP f

usion

an

tigen

reco

mbin

ant d

eam

idate

d glia

din ep

tides

(Zed

ira, G

erm

any)

DGPx

126

-mer

ic gli

adin

epito

pe

DGPx

233

-mer

ic +

26-m

eric

epito

pe

DGPx

433

-mer

ic +

26-m

eric

epito

pe +

DQ2

epito

pes

16


Fig 3.1.1 Epitope map of anti-gliadin antibodies

Data obtained based on cross-inhibition of binding of biotinylated antibodies to immobilized antigen. Numbers show XGY clone Id’s and suggested epitope groups (I, II, III, IV, V?).Repeated circles in XGY1 and XGY6 show suggested multiple epitopes, these mAbs show no self-inhibition.Group V is not confirmed by cross-inhibition: mAbs XGY3 and XGY4 do not inhibit other mAbs and are not inhibited by any of the mAbs.RT1 and RT2 are the mapping location of tracer antibodies from reference ELISA kits.

Fig. 3.1.2, 3.1.3 Monoclonal antibodies against gliadin – blotting, staining by Ponceau

omega

gamma

beta

alpha

ponceau 4 8 2 9 1 6mAbs XGY codes ascitic

16 4 7

20

Ponceau

14

11

10

15

13

19

18

12

17

mAbs XGY codes ascitic

I

SDS-PAGE 13 % gelBlotted mAb stained by anti-murine HRP and DAB chromogen.

Fig. 3.1.4 Monoclonal antibodies against recombinant fusion gliadin peptides– blotting, silver stained

Performed by Zedira GmbH, www.zedira.deAntigens: recombinant gliadin fusion proteins DGPx1, DGPx2, DGPx4, natural gliadinLeft panel: mAb XGY6; right panel: mAb XGY11

17


Fig. 3.1.5 Monoclonal antibodies against gliadin – immunohistochemistry

Rabbit Antibodies to sesame seed, neat antiserum

Cat # AS389

Host Rabbit

Immunogen Heated sesame seed extract

Specificity Highly reactive to all cereals

Application Immunoassays

3.2. Antibodies to soy and other legumes

Murine monoclonal antibodies to human soybean trypsin inhibitor (SBTI)

Cat # X259, X832, X841, X842

Isotype IgG1

Immunogen Purified SBTI

Specificity All antibodies selected for high affinity for SBTI

Application Immunoassay pairs: X259 or X832 vs X841 or X842

Murine monoclonal antibodies to soy glycinin

Cat # X16

Isotype IgG

Immunogen Heated soy extract

Specificity Acid glycinin peptide A5, 14 kDa (see Fig.3.2.1)


Paraffin-embedded section of human duodenum (duodenitis) stained by biotinylated mAb XGY2, streptavidin-HRP and DAB chromogen

18



Cat # X35

Isotype IgG


Specificity Basic glycinin peptide, 20 kDa (see Fig.3.2.1)



Cat # X49,X67,X68,X78,X84,X258, X285, X295

Isotype IgG


Specificity Acid glycinine peptides A1-A4 and basic glycinine peptide B3



Cat # X116

Isotype IgG


Specificity Conglycinin (72-70 kDa)+ acid glycinin A1-4

Application Immunoassays, matched to polyclonal antibody Cat# AS384 A


Cat # X292

Isotype IgG1


Specificity Acid glycinin peptides A1-A4, 40-45 kDa (see Fig.3.2.1)

Application Immunoassays, matched to polyclonal antibody Cat# AS384 A

Murine monoclonal antibodies to soy conglycinin

Cat # X89, X93

Isotype IgG


Specificity Conglycinin alpha, 60-70 kDa

Application Immunoassays, matched each other or to polyclonal antibody Cat# AS384 A

19


Fig. 3.2.1. Monoclonal antibodies to soy proteins- blotting.X16 X35 X89 X292 Ponceau

C G

GG G GG

C

C CC C C

SDS-PAGE 13 % gel, w/o 2-MEBlotted m Conglycinin (72-70 kDa)+ acid glycinin A1-4 Ab stained by anti-murine HRP and DAB chromogenProteins (see bottom):C – conglycinin; G- glycinin

Rabbit Antibodies to soybean antigens, affinity purified on antigen

Cat # AS384 A

Host Rabbit

Immunogen Raw soy extract

Specificity Weak cross-reactivity to peanut

Application Immunoassays for species-specific detection of soybean glycinin or conglycinin

Rabbit Antibodies to legumes, neat antiserum

Cat # AS384X

Host Rabbit

Immunogen Mixed extract of green pea, white bean and lentil

Specificity Mild cross-reaction to soybean and peanut


3.3. Antibodies to vegetables, fruits and spices

Murine monoclonal antibodies to orange See table 3.3. below


Isotype IgG

Immunogen Fresh orange pulp extract

Specificity Various, see table

Application Immunoassays for detection of orange, citrus fruits or common fruit antigens

20


Table 3.3. Monoclonal antibodies against orange

Cross -reactivities

Clone Id Fresh orange extract Orange juice Pairs for orange

juiceLemon

peel Peach juice Apple juice

X391 +++ neg no 2+ neg neg

X392 +++ neg no neg neg neg

X399 neg 3+ X395 neg 3+ neg

X395 + 3+ X399 neg 3+ neg

X396 +++ neg X395, X399 neg neg neg

Murine monoclonal antibodies to buckwheat

Cat # not yet established, 5+ clones

Isotype: IgG

Immunogen: fresh buckwheat extract

Specificity: buckwheat specific

Application: Immunoassays for detection of buckwheat

Murine monoclonal antibodies to garlic

Cat # X402, X417,X410,X420,X409,X404

Isotype: IgG

Immunogen: fresh garlic extract

Specificity: garlic specific

Application: Immunoassays for detection of garlic

Murine monoclonal antibodies to garlic and onion

Cat # X400,X408,X414,X415,X419

Isotype: IgG

Immunogen: fresh garlic extract

Specificity: specific for Allium species (garlic and onion)

Application: Immunoassays for detection of garlic and onion

Rabbit Antibodies to garlic/onion, neat antiserum

Cat # AS395G

Host Rabbit

Immunogen Fresh garlic extract

Specificity Cross-reactive to various plant extracts

Application Immunoassays for detection of garlic and onion

21


Murine monoclonal antibodies to mustard

Cat # XM3,XM14,XM64,XM79

Isotype: IgG

Immunogen: fresh mustard extract

Specificity: mustard specific

Application: Immunoassays for detection of mustard

Rabbit Antibodies to celery, neat antiserum

Cat # AS395C

Host Rabbit

Immunogen Fresh celery root extract

Specificity Cross-reactive to various plant extracts

Application Immunoassays for detection of celery

3.4. Antibodies to nuts

Rabbit Antibodies to walnut/pecan, neat antiserum

Cat # AS371

Host Rabbit

Immunogen Raw walnut extract

Specificity Highly reactive to walnut and pecan, cross-reactive to other nuts


Monoclonal antibodies to hazelnutSee table 3.4.1. below


Isotype IgG1

Immunogen Heated hazelnut extract

Specificity See table for details


22


Table 3.4.1. Monoclonal antibodies against hazelnut

Clone Id Cross-reactivity immunoassay pairs

X380 specific for hazelnut X382, AS372

X381 XR walnut X380, X382, AS372

X382 strong XR brazil nut, XR walnut X386, AS372

X383 XR walnut X380, X382, X386, AS372

X384 XR walnut, cashew, brazil X380, X382, AS372

X385 strongly prefers raw nut XR walnut not found

X386 specific for hazelnut X380, X382, AS372

X387 strong XR walnut and cashew X380, X382, AS372

X388 strong XR peanut, brazil nut; XR walnut X380, X382, AS372

X389 XR walnut X380, X382, AS372

Rabbit Antibodies to hazelnut, neat antiserum

Cat # AS372

Host Rabbit


Specificity Highly reactive to hazelnut, cross-reactive to other nuts


Rabbit Antibodies to hazelnut, affinity purified on antigen

Cat # AS372 A

Host Rabbit


Specificity Highly reactive to hazelnut, weak or no cross-reactive to other nuts


Monoclonal antibodies to peanut See table 3.4.2. below


Isotype IgG1

Immunogen Heated peanut extract



23


Table 3.4.2. Monoclonal antibodies against peanut

Cross-reactivity to nuts

Clone Id Comment on specificity Walnut Hazelnut Almond Brazil nut Immunoassay pairs

X377 raw peanut only - - - - AS373

X378 raw peanut only - - - - AS373

X373 XR almond (cooked only) 3+ + - - -

AS373, X370, X374, X375, X376, X377,

X378, X379

X375 strongly prefers raw peanut ++ - - -

X379heat resistant, XR almond

3+, cashew 2+, macadamia, brazil +-/1+

+ - +- + X370

X374 strong XR to macadamia - - - -

X370 raw peanut only - - - - X379

X376 heat resistant - - ++ - X373, AS373

Rabbit Antibodies to peanut, neat antiserum

Cat # AS373

Host Rabbit


Specificity Highly reactive to peanut, cross-reactive to other nuts


Rabbit Antibodies to peanut, affinity purified on antigen

Cat # AS373 A

Host Rabbit


Specificity Highly reactive to peanut, weak or no cross-reactive to other nuts


24


Monoclonal antibodies to almondSee table 3.4.3. below


Isotype IgG1

Immunogen Heated almond extract



Table 3.4.3. Monoclonal antibodies against almond

Clone Id Species specificity Comment on speciicity by Western blotting Immunoassay pairs

X50 common nuts antigen main storage protein (prunin) X147, X303, X335, X135

X135 almond specific weak staining of main storage protein, pattern X303/335 X50, self, X162

X140 XR pecan 2+ not found

X141 almond specific

X144 almond specific not found

X145 XR hazelnut 1+, coconut 4+ not found

X146 no data not found

X147 almond specific X50, X151

X151 almond specific weak staining of main storage protein, pattern X303/335 X147

X162 almond specific strong staining of main storage protein, pattern X162 X335, self


X303 no data weak staining of main storage protein, pattern X303/335 X50


X309 XR Walnut no

X335 XR hazelnut 2+ , Pecan 1+ strong staining of main storage protein, pattern X303/335 X50, X135, X162

25


Fig. 3.4. Monoclonal antibodies to almond- blotting.

W/o ME

9766

45

30

20

14

MW, kD

CT

RL

X303

X335

X142

X162

X135

X151

9766

45

30

20

MW, kD

X142

X18

X162

X135

X303

X151

X335

Ag

Ag

CT

RL

SDS-PAGE 13 % gel, w/o 2-ME (left panel) or with 2-ME (right panel)Blotted mAb stained by anti-murine HRP and DAB chromogenAg – preparation of raw almond extractmAb X18 (anti-albumin) was tested as control of nonspecific binding to the antigen

Rabbit Antibodies to almond, neat antiserum

Cat # AS374

Host Rabbit


Specificity Highly reactive to almond, cross-reactive to other nuts


Rabbit Antibodies to almond, affinity purified on antigen

Cat # AS374 A

Host Rabbit


Specificity Highly reactive to almond, weak or no cross-reactive to other nuts


Rabbit Antibodies to macadamia, neat antiserum

Cat # AS375

Host Rabbit

Immunogen Heated macadamia extract

Specificity Highly reactive to macadamia, cross-reactive to other nuts


26


Rabbit Antibodies to cashew, neat antiserum

Cat # AS376

Host Rabbit

Immunogen Heated cashew extract

Specificity Highly reactive to cashew, cross-reactive to other nuts


Rabbit Antibodies to brazil nut, neat antiserum

Cat # AS378

Host Rabbit

Immunogen Heated brazil nut extract

Specificity Highly reactive to brazil nut, cross-reactive to other nuts


Rabbit Antibodies to coconut, neat antiserum

Cat # AS379

Host Rabbit

Immunogen Heated coconut extract

Specificity Highly reactive to coconut, cross-reactive to other nuts


3.5. Antibodies to mushrooms

Monoclonal antibodies to death angel mushroom (Amanites spp)

Cat # Same as Clone Id

Isotype IgG

Immunogen Mycelial extract of Amanites fungus

Specificity See table below; non-reactive to edible mushrooms


Clone Id phalloides virosa

SBP4 ++ ++

SBP8 ++ +++++

SBP14 ++ +/-

SBP17 +/- +

SBP18 +/- +

27


Rabbit Antibodies to death angel mushroom (Amanites spp), affinity purified on antigen

Cat # AS891 A

Host Rabbit

Immunogen Extract of Amanites virosa

Specificity Cross-reactive to other mushrooms


3.6. Antibodies to Crustacea

Rabbit Antibodies to Crustacea, affinity purified on antigen

Cat # AS350 A

Host Rabbit

Immunogen Extract of boiled shrimp

Specificity Cross-reactive to all Crustacea and most of Arthropodae


Murine monoclonal antibodies to Crustacea

Cat # SKR1, SKR3, SKR4, SKR5, SKR6

Isotype IgG


Specificity Common 42 kDa protein of Crustacea, cross-react to most of Arthropodae

Application Immunoassay pairs: all mAbs against AS350; SKR1 vs SKR3 or SKR4

Fig. 3.6. Monoclonal antibodies to shrimp - blotting

CT

RL

SK

R6

SK

R3

SK

R5

9766

45

30

20

14

MW, kD S S S S SG G G G G

SDS-PAGE 13 % gel, w/o 2-ME Blotted mAb stained by anti-murine HRP and DAB chromogen

Antigens: S – boiled shrimp extract; G – heated antigen of gadfly

28


3.7. Antibodies to fish, amphibiae and lamprey

Monoclonal antibodies to bone fishSee table 3.7.1. below


Isotype Various, see table

Immunogen Boiled codfish extract



Table 3.7.1. Monoclonal antibodies against bone fish

Clone Id Isotype Species specificity

X21 IgG2b all bone fishes

X09 IgG1 selected fishes

X36 IgG2a/2b selected fishes, raw only

X37 IgG1/ IgG2a selected fishes

X65 IgG1 selected fishes

X80 IgG2a selected fishes, prefers cooked material, surimi 2+, frog 2+

X83 IgG2a selected fishes, raw only

X85 IgG2a selected fishes, prefers cooked material

X185 IgG2a selected fishes

Rabbit Antibodies to bone fish, affinity purified on antigen

Cat # AS363 A

Host Rabbit

Immunogen Extract of boiled codfish

Specificity Reactive to muscle tissue and innards of all bone fish species, cross-reactive to cartilaginous fishes and amphibiae (frog)


29


Rabbit Antibodies to bone fish, affinity purified on antigen, adsorbed on amphibiae

Cat # AS363 AX

Host Rabbit

Immunogen Extract of boiled codfish

Specificity Reactive to muscle tissue and innards of all bone fish species, weakly cross-reactive to cartilaginous fishes


Rabbit Antibodies to hydrolyzed bone fish (surimi), affinity purified on antigen

Cat # AS363KP A

Host Rabbit

Immunogen Extract of surimi (hydrolyzed codfish)

Specificity Reactive to hydrolyzed muscle tissue and innards of all bone fish species, cross-reactive to cartilaginous fishes


Monoclonal antibodies to cartilaginous fishSee table 3.7.2. below


Isotype Not determined

Immunogen Boiled mixed shark and sturgeon extract



Table 3.7.2. Monoclonal antibodies against cartilaginous fish

Clone Id Species specificity Immunoassay pairs

X24 selected fishes

X74 sturgeon, selected sharks and bone fish

X98 Selected sharks

X277 Sturgeon specific vs X315: sturgeon assay

X08 Selected sharks

X315 selected sharks, sturgeon vs X235: sturgeon assay

X219 selected sharks, sturgeon

X79 selected sharks

30


Table 3.7.2. Monoclonal antibodies against cartilaginous fish (continued)

Clone Id Species specificity Immunoassay pairs

X38 all fishes, strongly prefers cooked material

X64 selected sharks, sturgeon


X184 selected fishes, frog

X236 selected fishes



X88 selected fishes

X45 selected fishes

X53 selected fishes

X252 selected sharks, sturgeon vs X315: sturgeon assay

X235 sturgeon specific vs X315: sturgeon assay


Rabbit Antibodies to shark, neat antiserum

Cat # AS363A

Host Rabbit

Immunogen Extract of boiled Tunisian gray shark

Specificity Reactive to muscle tissue and innards of all sharks species, cross-reactive to other cartilaginous fishes, weak cross-reaction to bone fishes and amphibiae (frog)


Rabbit Antibodies to shark, affinity purified on antigen

Cat # AS363A A

Host Rabbit

Immunogen Extract of boiled Tunisian gray shark



31


Rabbit Antibodies to sturgeon, neat antiserum

Cat # AS363Y

Host Rabbit

Immunogen Extract of boiled sturgeon



Rabbit Antibodies to sturgeon, affinity purified on antigen

Cat # AS363Y A

Host Rabbit

Immunogen Extract of boiled sturgeon



Rabbit Antibodies to lamprey, neat antiserum

Cat # AS363M

Host Rabbit

Immunogen Extract of boiled lamprey

Specificity Reactive to muscle tissue and innards of lamprey, weak cross-reaction to fishes


32


3.8. Avian meats, eggs and other tissues

Murine monoclonal antibodies to avian tissue and blood antigens

Cat # SV1

Isotype IgG1

Immunogen Boiled hen’s egg white

Specificity Not determined, heat-resistant, species specific for hen

Application Immunoassays for species-specific detection of hen’s egg

Cat # SV2

Isotype IgG1


Specificity Not determined, heat-resistant component of avian blood, species nonspecific

Application Immunoassays for avian components

Cat # SCH8

Isotype No data

Immunogen Boiled chicken meat

Specificity Tropomyosin complex of chicken meat, weakly reactive to mammal meat, nonreactive to fish and frog


See table 3.8. below


Isotype IgG


Specificity Ovotransferrin (conalbumin), heat-resistant component of avian eggs, varies species nonspecific (see table)


33


Table 3.8. Monoclonal antibodies against ovotransferrin

Resistance to heating:

Clone Id Boiling 15’ Pan frying 15’ Resistance to drying Species coverage

X61 yes no no Pheasant, duck, austrich negative

X63 no no yes Pheasant, duck, austrich weak

X55 no no no quail, turkey negative, duck weak

X60 yes no no Hen specific

X62 no no no Hen specific

X66 yes no yes Duck negagive

X71 yes yes yes Duck, pheasant negative

X76 yes yes yes Duck, pheasant negative

X119 yes +- +- Duck, pheasant negative

X136 no no no Hen specific

X183 yes yes yes Pan-egg, weak turkey, quail

X190 yes +- no variable, depends on cooking

X199 yes no no Hen and turkey only

X208 yes no no Duck negative

X209 yes yes +- Duck weak

Rabbit Antibodies to egg white, affinity purified on antigen

Cat # AS361 A

Host Rabbit


Specificity Reactive to all major egg white allergens, no cross-reaction to chicken meat

Application Immunoassays for egg white proteins

34


Fig. 3.8. Species-specific monoclonal antibodies to egg white extract- Western blotting

Ovotransferrin76 kD

Ovalbumin45 kD

As

36

1 (

111

)

X5

5X

60

X6

2

X6

6

X7

6X

71

X11

9X

13

6

X1

83

X1

90

X1

99

X2

08

X2

09

Sv

2

1 2

SDS-PAGE 13 % gel, w/o 2-MEBlotted mAbs (see clone Id’s on top) stained by anti-murine HRP and DAB chromogen

3.9. Mammal meats and other tissues

Murine monoclonal antibodies to porcine serum albumin

Cat # X27, X28, X34, X39, X51, X56, X59

Isotype IgG1

Immunogen Porcine tissues

Specificity All antibodies selected for species-specific binding of porcine fat tissue and blood, nonreactive to blood and fat of other mammals

Application Immunoassays for species-specific detection of porcine blood, fat and innards*

*Some of the mAbs are only sold in terms of License Agreement

Fig. 3.9.1. Species-specific monoclonal antibodies to porcine serum albumins – blotting

Ponceau

X04

X51

X59

X56

X12

Control

9766

45

30

20

14

MW, kD PP P P P P PB B BOv Ov

SDS-PAGE 13 % gel, w/o 2-MEBlotted mAbs (see clone Id’s on top) stained by anti-murine HRP and DAB chromogenAlbumins (see bottom):B – bovine; Ov- ovine; P - porcine

35


Murine monoclonal antibodies to meat-specific antigens (components of skeletal muscle tissue). See table 3.9.1. below


Isotype IgG

Immunogen Meat extracts

Specificity All antibodies selected for high affinity binding to raw and cooked meat extracts

Application Immunoassays for species-specific and nonspecific detection of meat extracts*


Table 3.9.1. Monoclonal antibodies to meat-specific antigens - UPDATED

Clone Id Species specificity Suggested target Blotting

X641reactive against striated muscular tissue of

worms, molluscs, fish, amhibia, reptilia, birds, mammals

unknown compo-nent of tropomyosin

complexno data

X643

reactive against striated muscular tissue of fish, amphibia, reptilia, birds, mammals

in most of meats prefers COOKED material - marker of cooking


complexno data

X646

reactive against striated muscular tissue of birds, mammals

in most of meats prefers COOKED material - marker of cooking


complexno data

X214 reactive against pork; weakly reactive to several other meats

25 kDa (adenylatki-nase) Fig.3.9.5

X229 reactive against all meats, prefers raw meat 25 kDa (adenylatki-nase) Fig. 3.9.3

X264 reactive against pork, lamb; weakly reactive to several other meats high MW glycoprotein Fig. 3.9.3

X279 reactive against pork, lamb, horse; weakly reactive to several other meats 45/65 kDa protein Fig. 3.9.3

X232 reactive against striated muscular tissue of all mammals high MW glycoprotein Fig. 3.9.4

36


Table 3.9.1. Monoclonal antibodies to meat-specific antigens (continued)

Clone Id Species specificity Suggested target Blotting

XP53 prefers pork; reactive to several other meats tropomyosin Fig.3.9.5

X70 strictly pork meat specific ultra heat resistant high MW glycoprotein

Fig. 3.9.3, 3.9.5

X371 strictly pork meat specific ultra heat resistant high MW glycoprotein not shown

X372 strictly pork meat specific ultra heat resistant high MW glycoprotein not shown

Beef-negative murine monoclonal antibodies to meat-specific antigens (components of skeletal muscle tissue). See table 3.9.2. below


Isotype: IgG

Immunogen: Elk meat extract

Specificity: All antibodies selected for negative reaction to beef extract

Application: Immunoassays

Table 3.9.2. Beef-negative monoclonal antibodies to meat-specific antigens

Clone Id Isotype Species specificity Target by blotting immunoassay pairs

X100 IgG1 Horse / Elk / Chicken unknown X109, X134

X176 no data Horse / Elk 46>32 kDa (elk) X188

X101 no data Horse/ Elk / Dog unknown X188,X112

X112 IgG1 non-reactive in direct binding unknown X104, X105, X109, X134, X166, X101

X128 no data Horse/ Elk / Chicken unknown not found

X131 no data Horse / Elk / Chicken unknown not found

X178 no data Horse/ Elk / Dog unknown not found

X182 no data Horse/ Elk / Chicken unknown not found

X198 IgG1 Horse/ Elk / Chicken unknown not found

37


Table 3.9.2. Beef-negative monoclonal antibodies to meat-specific antigens (continued)

Clone Id Isotype Species specificity Target by blotting immunoassay pairs

X106 no data weakly reactive in direct binding unknown not found

X170 no data Horse/ Elk / Dog / Chicken unknown not found

X103 no data non-reactive in direct binding 20/27/35 kDa not found

X129 no data Horse/ Elk / Dog unknown not found

X142 IgG1 Horse/ Dog unknown not found

X104 no data All meats strong unknown X112, X166

X105 no data All meats strong unknown X112, X166

X143 IgG2a All meats strong unknown not found

X109 IgG1 weak Horse / Sheep / Chicken unknown X100, X112, X188 weak

X134 IgG1/ IgG2a mammals+ chicken (raw only) unknown

X100, X101, X112, X157, X166, X176,

X198, AS364

X188 IgG1 Horse / Sheep, weak poultry 20/27/35 kDa

X101, X109, X112, X130, X133, X176

X177 no data Horse / Chicken unknown X104, X105

X197 IgG1/ IgG2a All meats strong unknown not found

X157 IgG2a Horse / Elk / Sheep strong unknown not found

X166 no data Horse / Elk / Sheep / Dog/ Chicken

17/22/31 kDa (Elk) X112

X130 IgM Horse / Elk / Sheep / Dog/ Chicken

43/55 kDa (Elk) X188

X133 IgG1/ IgG2a Horse/ Elk / Chicken unknown X188

38


Fig. 3.9.2. Beef-negative monoclonal antibodies to meat tissue components – blotting

D

A B

C

PonceauPonceau

PonceauPonceau

9766

45

30

20

14

MW, kD

9766

45

30

20

14

MW, kD

9766

45

302014

MW, kD

9766

45

30

20

14

MW, kDE B S H

Control X166

E B S H E B S H

EB S HE BSH

E BSH E BSH

E BSH

E BSH

E BSH

EB S H EB S H

X188 X103X130

X232 X76

SDS-PAGE 13 % gel, with 2-MEBlotted mAbs (see clone Id’s on top of each part) stained by anti-murine HRP and DAB chromogenRaw meat extracts (see bottom):E – elk; H – horse; S – lamb; B – beef (negative control for all mAbs except mAb X232)

Fig. 3.9.3. Monoclonal antibodies to meat tissue components – blotting

9766

45

30

20

14

MW, kD

X264 X70 X279 X229Ponceau

P B S P B S P B S P B S P B S

SDS-PAGE 13% gel, with 2-MEBlotted mAbs (see clone Id’s on top of each part) stained by anti-murine HRP and DAB chromogenRaw meat extracts (see bottom):P - pork; B – beef; S – lamb

39


Fig. 3.9.4. Monoclonal antibody X232 to all mammal meats – blotting

976645

30

20

14

MW, kD

Ponceau X232

P S B E H P S B E H F C

SDS-PAGE 13% gel, without 2-MEBlotted mAb X232 stained by anti-murine HRP and DAB chromogenRaw meat extracts (see bottom):P - pork; S – lamb; B – beef; E – elk; H – horse; F – codfish (control); C – chicken (control)

Fig. 3.9.5. Monoclonal antibodies to cooked pork meat – blotting

97

66

45

30

20

14

MW, kD

X7

0

CT

RL

X2

32

X2

14

Xp

53

Po

nc

ea

u

SDS-PAGE 13 % gel, with 2-MEBlotted mAbs (see clone Id’s on the top) stained by anti-murine HRP and DAB chromogen

40


3.10. Antibodies to milk antigens

Murine monoclonal antibody to milk casein

Cat # X113

Isotype IgG1

Immunogen Hydrolyzed cow casein

Specificity Milk casein of various species

Application Immunoassays for casein

Murine monoclonal antibodies to lactalbumin See table 3.10. below


Isotype IgG

Immunogen Milk fractions

Specificity All antibodies selected for high affinity binding to milk fractions

Application Immunoassays for species-specific and nonspecific detection of milk*


41


Table 3.10. Monoclonal antibodies to milk-specific antigens

Cone Id Dried milk Fresh milk Goat milk BSA Human milk

XM29 4+ neg neg neg neg

XM21 4+ 2+ 2+ neg neg

XM23 4+ 2+ 1+ neg 1+

XM24 4+ 2+ 2+ neg 2+

XM34 4+ 2+ 1+ neg 1+

XM35 2+ 2+ 1+ neg 2+

XM37 2+ 2+ 1+ neg 1+

XM36 3+ 2+ 1+ neg neg

XM28 4+ 3+ +- neg neg

XM33 4+ 3+ +- neg 3+

XM42 4+ 4+ 1+ neg neg

XM43 4+ 4+ 1+ neg neg

XM44 4+ 4+ 1+ neg neg

XM45 4+ 4+ 1+ neg neg

XM47 4+ 4+ 1+ neg neg

XM50 2+ 2+ 2+ neg neg

XM51 2+ 2+ 2+ neg 3+

XM52 2+ 2+ 2+ neg neg

XM53 2+ 2+ 2+ neg neg

XM54 4+ 3+ 3+ neg 2+

XM55 2+ 2+ 2+ neg neg

XM56 3+ 2+ 3+ neg neg

XM57 3+ 2+ 3+ neg neg

XM58 4+ 4+ 4+ 4+ 2+

XM59 4+ 3+ 4+ neg 2+

XM60 2+ 2+ 1+ neg 2+

XM61 3+ 3+ 1+ neg 2+

42


4. Antibodies against biologicals for agriculture and environmental control

4.1. Rabbit Antibodies to Ralstonia species

Cat # AS861G,R,Y

Host Rabbit

Immunogen LPS of Ralstonia


Serotype specificity:

Cat# Target serotype Cross-reactive serotypes

AS861G RS7944 no

AS861R RS5712 no

AS861Y RS7859 RS7944

4.2. Rabbit Antibodies to fungal antigens

Cat # See table 4.2

Host Rabbit

Immunogen Mycelial extracts


43


Table 4.2. Rabbit antibodies to fungal antigens

Cat # Target genus Species specificityAffinity puru-

fied antibodies available

AS821F Aspergillus fumigatus, flavus, ochraceus yes

AS821N Aspergillus niger, carbonarius yes

AS824 Mucor, Rhizopus M.pusillus, R.nigricans yes

AS825 Phoma betae yes

AS826 Alternaria alternata yes

AS827 Fusarium various yes

AS829 Phytophtora common to all Oomycetes no

AS834 Botrytis alternata no

AS835 Pythium common to all Oomycetes no

AS836 Phaeoacremonium various no

AS840 Monascus alternata no

AS844 Claviceps (Ergot) various yes

AS845 Ascochyta cucumeris no

Fig. 4.2. Immunocytochemical staining of Rhizopus nigricans by rabbit antiserum

Fungal cells stained by antiserum, second anti-rabbit HRP and DAB chromogen

Monoclonal antibodies to Amanites spp


Isotype IgG

Immunogen Mycelial extract of Amanites fungus

Specificity See table below; non-reactive to edible mushrooms


44


Clone Id Phalloides Virosa

SBP4 ++ ++

SBP8 ++ +++++

SBP14 ++ +/-

SBP17 +/- +

SBP18 +/- +

4.3. Rabbit Antibodies to arthropodal antigens

Rabbit Antibodies to Dermatophagoides spp. antigen, neat antiserum

Cat # AS851

Host Rabbit

Immunogen Dermatophagoides pteronyssinus extract

Specificity Reactive to Dermatophagoides species mite extracts, cross-reaction to other mites and insects

Application Immunoassays for house dust mites

Rabbit Antibodies to Dermatophagoides spp. antigen, affinity purified on antigen

Cat # AS851 A

Host Rabbit

Immunogen Dermatophagoides pteronyssinus extract

Specificity Reactive to Dermatophagoides species mite extracts, weak cross-reaction to other mites, no cross-reactions with insects and crustaceans

Application Immunoassays for house dust mites

Murine monoclonal antibodies to Arthropodae

Cat # SKR1, SKR3, SKR4, SKR5, SKR6

Isotype IgG


Specificity Common 42 kDa protein of Crustacea, cross-react to most of Arthropodae

Application Immunoassay pairs: all mAbs against AS350; SKR1 vs SKR3 or SKR4

45


Rabbit Antibodies to arthropodal antigens

Cat # AS852

Host Rabbit

Immunogen Body extract of Treboluim castanei

Specificity Reactive to larvae, imago and feces of most Arthropodae

Application Immunoassays for insect contamination of foods

Table 4.3. Antibodies to arthropodal antigens

Species Order Family SKR1 SKR3 SKR4 SKR5 AS852

Chalcophora mariana Coleoptera Buprestidae 3+ neg neg neg neg

Cetonia aurata L. Coleoptera Scarabaeidae neg 1+ 1+ neg 2+

Melolontha hippocastani Coleoptera Scarabaeidae 2+ neg neg neg neg

Leptinotarsa decemlineata Coleoptera Chrysomelidae neg 1+ 1+ 1+ 2+

Graphosoma lineatum Heteroptera Pentatomidae 2+ +- neg neg 1+

Eurygaster sp. Heteroptera Scutelleridae neg 1+ 1+ neg 2+

Nepa cinerea Heteroptera Nepidae 4+ 2+ 2+ neg 1+

Gerris rufoscutellatus Heteroptera Gerridae neg +- neg neg 3+

Apis mellifera Hymenoptera Apidae 3+ 2+ 2+ 2+ 1+

Polister nimpha Hymenoptera Vespidae neg 2+ 2+ neg 2+

Gomphus megatissimus Odonata Gomphidae 3+ 2+ 2+ neg 2+

Calopteryx splendens Odonata Calopterygidae 1+ neg neg neg 3+

Platycnemis pennipes Odonata Platychemididae neg 3+ 3+ neg neg

Panorpa communis Mecoptera Panorpidae neg neg neg neg neg

Hybomitra Diptera Tabanidae +- 4+ 4+ neg 1+

Myrmeleon sp. Neuroptera Myrmeleontidae neg 2+ 2+ neg neg

Ectobius lapponicus Blattodea Blatellidae neg 3+ neg neg neg

Nauphoeta cinerea Blattodea Blaberidae neg 1+ 1+ neg neg

Raphidia sp. Raphidioptera Raphidiidae neg 1+ neg neg neg

46


5. Antibodies and conjugates for detection of LMW substances

5.1. Rabbit Antibodies to antibiotics, neat antisera

Cat # See table 5.1.

Host Rabbit

Immunogen See table 5.1.

Specificity Item and lot dependent, please inquire


Table 5.1. Immunoassay reagents for determination of antibiotics

Available reagents for immunoassays

Cat# Drug or drug class Immunogen BSA con-jugate

Ovalbu-min coin-

jugate

SBTI conjugate

HRP conju-gate

AS902 Chloramphenicol Chloramphenicol-NHS-BSA yes no no yes

AS905 Streptomycin Streptomycin-CONH-BSA yes yes no no

AS906 Tylosin Tylosin-CONH-BSA yes yes no no

AS909 Ciprofloxacin Ciprofloxacin-CONH-cBSA yes yes no no

AS930 Sulfonamides Sulfonamide-CH2-NH2-BSA yes yes yes no

5.2. Murine monoclonal antibodies against mycotoxins


Isotype See table 5.2.

Immunogen Conjugated mycotoxin

Specificity See table 5.2.

Application Immunoassays, immunoadsorbent columns

47


Table 5.2. Immunoassay reagents for determination of mycotoxins

All antibodies applicable for ELISA

Clone Id Isotype Target antigen Available conjugates

XJ978 IgG1 Aflatoxin B BSA, HRP

XM922 IgG1 T-2 toxin BSA

XC923 IgG1 Zearalenone (ZEN) BSA, Ovalbumin, HRP

XJ924 IgG1 Ochratoxin (OTA) BSA, Ovalbumin

XM925 IgG1 DON BSA, SBTI

XVC3 IgG1 Fumonisin BSA, Ovalbumin

5.3. Rabbit Antibodies to plant steroids (brassinosteroids), neat antisera

Cat # See table 5.3.

Host Rabbit

Immunogen See table 5.3.

Specificity Item and lot dependent, please inquire


Table 5.3. Immunoassay reagents for determination of plant steroids

Available reagents

Cat# Target analyte Immunogen Immunoreactivity BSA

conj

ugat

e

HRP

conj

ugat

eAS611 24S-methyl-

brassinosteroidsCastasterone-CMO-

BSA Brassinolide, castasterone yes yes

AS612 24R-methyl-brassinosteroids

24-Epicastasterone-CMO-BSA

24-epibrassinolide 24-epicastasterone

24-epi-6-deoxocastasteroneyes yes

AS613 24S-ethylbrassi-nosteroids

28-Homo-castasterone-CMO-

BSA

28-homobrassinolide 28-Homocastasterone yes yes

AS614 lactone-type brassinosteroids

28-Norbrassinolide-HS-BSA

brassinolide, 24-epibrassinolide,

28-homobrassinolide 28-norbrassinolide

yes yes

AS615 ketone-type brassinosteroids

28-Norcastasterone-HS-BSA

28-norcastasterone, 24-epicastasterone,

28-homocastasterone, castasterone

yes yes

48


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agriculture and environment testing - xema-medica.com reagents catalogue 2015.pdf · 3 ˜˚˛ 2015...

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Transcript of agriculture and environment testing - xema-medica.com reagents catalogue 2015.pdf · 3 ˜˚˛ 2015...