Agilent Intuvo 9000 GC/MS · 2019. 2. 8. · 2 1. GC/MS Agilent Agilent Fiehn GC/MS RTL UC Davis...
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Agilent Intuvo 9000 GC/MS GC/MS GC/MS Key word , GC/MS Authors 1 , 1 , 2 , 1 1. 2.
Transcript of Agilent Intuvo 9000 GC/MS · 2019. 2. 8. · 2 1. GC/MS Agilent Agilent Fiehn GC/MS RTL UC Davis...
Agilent Intuvo 9000 GC/MS
GC/MS
GC/MS
Key word , GC/MS
Authors
1, 1,
2, 1
1.
2.
2
1.
GC/MS
Agilent
Agilent Fiehn GC/MS RTL
UC Davis Oliver Fiehn
37.5
Van
Deemter
20~30cm/s
35~40cm/s
Agilent
JetClean
2
2.
1.5 mL
10 L
(20mg/mL ) 37 90
45 L MSTFA+1%TMCS
37 30
GC-MS : Agilent Intuvo 9000 GC/5977B MSD
DB-5ms Intuvo GC 30 m
0.25 mm, 0.25 m P/N: 122-5532-INT
: 1 L
: 250
: P/N: 5190-3169
: 10:1
: 250
: 150
MS : Scan (m/z: 50-600)
Precision Hydrogen Trace 500 cc for
Agilent (Peak Scientific instruments Ltd. )
: 60 (0.5 min)-20 /min-325 (5 min)
: 1.2 mL/min
:
: 60 (1 min)-10 /min-325 (10 min)
: 0.6 mL/min
MS Agilent Fiehn GC/MS RTL
( : G1676AA)
3.
1
Agilent Fiehn GC/MS
RTL 76
TICC (Total Current Ion
Chromatogram) ( 1)
TICC
2
3
1 TICC
( )
He
H2
2
( )
3
( GABA+3TMS( ) Malate( )
m/z quantifier
qualifier )
2
Panc-1
AsPc1
2.0 106
10 cm dish 2 PBS
Ribitol 2-Isopropylmalate
10
3
30 2
1
4
Panc-1 AsPc1
4 ( )
( 1 1
Z-score 1, 2
Panc-1 AsPc1
)
(n=4 ) 1
(He/H2)
1
Ribitol
Compound Name He H2 He/H2
Met +2TMS 1.69 1.81 0.93
Pro +2TMS 0.26 0.36 0.71
Citrate +4TMS 1.56 0.65 2.40
Thr +3TMS 1.29 1.37 0.94
Ala +2TMS 2.47 2.35 1.05
Pro +TMS 0.3 0.37 0.81
Panc-1AsPc1
成分1 (46.9 %)
成分
2(2
3.0
%)
4
Leu +TMS 1.36 1.21 1.12
Leu +2TMS 1.11 1.17 0.95
Ile +2TMS 1.04 1.11 0.94
Succinate +2TMS 2.71 2.69 1.01
Ser +3TMS 1.82 1.79 1.01
Malate +3TMS 1.8 1.55 1.16
Asp +3TMS 11.76 15.08 0.78
Gly +3TMS 0.43 0.44 0.97
Glu +3TMS 1.19 1.05 1.14
Ribitol +5TMS
（tennka )
0.99 1.01 0.98
He H2
3
500
Presh-SPE ACX
5
( 5 )
5 500
( )
4.
GC/MS
12
ヘリウム
⽔素
1) 12 (2018)
2) GC/MS JetClean
Pub No.GC-MS-201712AZ-
001
3) GC/MS Agilent Fiehn GC/MS
RTL
Pub
No.low_5990-3638JAJP
4) JetClean
GC/MS
Pub No. GC-MS-201802SG-002
5) E. Takeo et al., Solid-phase analytical derivatization for
gas-chromatography-mass-spectrometry-based
metabolomics, J Biosci Bioeng. 2017 Dec,
24(6), 700-706
www.agilent.com/chem/jp
0120-477-111
© Agilent Technologies, Inc. 2019
Printed in Japan, February 7, 2019
GC-MS-201902HR-001
