Agilent GPC Data Analysis Software for Agilent ChemStation 4 GPC Data Analysis Software for...

Click here to load reader

download Agilent GPC Data Analysis Software for Agilent ChemStation 4 GPC Data Analysis Software for ChemStation

of 112

  • date post

    31-Jan-2021
  • Category

    Documents

  • view

    0
  • download

    0

Embed Size (px)

Transcript of Agilent GPC Data Analysis Software for Agilent ChemStation 4 GPC Data Analysis Software for...

  • Agilent GPC Data Analysis Software for Agilent ChemStation

    Installing and Understanding your GPC Data Analysis Software

    s1Agilent Technologies

  • GPC Data Analysis Software for ChemStation

    Notices © Agilent Technologies, Inc. 2006

    No part of this manual may be reproduced in any form or by any means (including elec- tronic storage and retrieval or translation into a foreign language) without prior agree- ment and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

    Microsoft ® is a U.S. registered trademark of Microsoft Corporation.

    Manual Part Number G2182-90020

    Edition 03/06

    Printed in Germany

    Agilent Technologies Hewlett-Packard-Strasse 8 76337 Waldbronn, Germany

    Software Revision This guide is valid for B.01.01 revisions of the Agilent GPC Data Analysis Software for Agilent ChemStation software,

    Warranty The material contained in this docu- ment is provided “as is,” and is sub- ject to being changed, without notice, in future editions. Further, to the max- imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par- ticular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connec- tion with the furnishing, use, or per- formance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep- arate agreement shall control.

    Technology Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accor- dance with the terms of such license.

    Restricted Rights Legend If software is for use in the performance of a U.S. Government prime contract or subcon- tract, Software is delivered and licensed as “Commercial computer software” as defined in DFAR 252.227-7014 (June 1995), or as a “commercial item” as defined in FAR 2.101(a) or as “Restricted computer soft- ware” as defined in FAR 52.227-19 (June 1987) or any equivalent agency regulation or contract clause. Use, duplication or disclo- sure of Software is subject to Agilent Tech- nologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will receive no greater than Restricted Rights as

    defined in FAR 52.227-19(c)(1-2) (June 1987). U.S. Government users will receive no greater than Limited Rights as defined in FAR 52.227-14 (June 1987) or DFAR 252.227-7015 (b)(2) (November 1995), as applicable in any technical data.

    Safety Notices

    CAUTION

    A CAUTION notice denotes a haz- ard. It calls attention to an operat- ing procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

    WARNING

    A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per- formed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated condi- tions are fully understood and met.

  • In This Guide… This manual will help you to familiarize yourself with the Agilent Technologies GPC data analysis software. Further, the manual describes in detail all the features and evaluation parameters of the software. The index at the end of the manual allows to search easily for certain keywords and gives quick access to necessary information. There is also extensive online help available within Agilent ChemStation and the GPC data analysis software. The manual contains the following information:

    1 Installing the GPC Data Analysis Software

    Requirements and procedures to install or uninstall the Agilent GPC data analysis software

    2 Basic Theory of GPC

    The importance of gel permeation chromatography (GPC) in polymer characterization has strongly increased since its introduction by Moore1 and others. Quality assurance and control—even during production and processing stages—have increased in significance to retain international competitive ability. Also, exact knowledge of molecular weights and their distribution is important in polymer research in academia and industry.

    However, support for users in evaluation of GPC data has not found the necessary interest in the past. The improvement of GPC software has not kept pace with improvements and redevelopments of GPC and liquid chromatography (LC) components. As a result even to today GPC data often have to be evaluated manually.

    Through the increasing automation of polymer analysis, GPC software today should be both flexible and powerful and simplify the workload of the user, making effective use of powerful and expensive hardware.

    GPC is characterized by a series of specific marginal conditions, some of which differ considerably from the conditions of LC:

    • completely different calibration procedure for transformation of elution volume in molar masses

    • completely different separation mechanism—diffusion controlled exclusion instead of adsorption equilibration

    • basically different requirements to the system parameters which determine the reproducibility, measurement and evaluation accuracy

    GPC Data Analysis Software for ChemStation 3

  • • variety of time bases—measurement time, peak width, and so on

    • variety of analysis goals—molecular mass specification instead of qualitative or quantitative analysis

    • multidetection in GPC suggests parallel evaluation of all used detectors

    • evaluation procedure should automatically process volume correction between the different detectors, so that only one calibration relation will be needed for all connected detectors

    3 Introduction to the GPC Data Analysis Software

    The Agilent GPC data analysis software integrates seamlessly into your existing Agilent ChemStation software and adds GPC data analysis features to it. The product directly uses the Agilent ChemStation support for Agilent instrumentation and fully utilizes its data acquisition and sample handling features. The Agilent GPC data analysis software supports conventional GPC analyses with multiple calibration methodologies:

    • narrow standard calibration

    • universal calibration

    • broad standard calibration

    • integral calibration

    • automated recalibration

    Data processing is limited to concentration detectors (like UV, DAD, RID or any signal detector connected via Agilent 35900E A/D converter); it does not handle light-scattering or viscometric detection, nor does it support special GPC evaluation strategies like copolymer analysis and chemical heterogeneity studies. Users who need that functionality should look for specialized GPC data systems. Agilent GPC data analysis software offers 3 different modes of GPC data processing:

    • automatic GPC data processing for single runs and sequences (on-line and off-line)

    • manual reprocessing of data files from the standard Agilent ChemStation data analysis

    • interactive reprocessing of GPC runs for fine-tuning demanding samples in the Agilent GPC data analysis software window.

    The Agilent GPC data analysis software is capable of multitasking and can run these modes in parallel. While one sequence of samples is analyzed and new data are acquired and reported, stored data files can be loaded and

    4 GPC Data Analysis Software for ChemStation

  • reprocessed starting from the standard Agilent ChemStation data analysis or from the interactive Agilent GPC data analysis software screen using different calibration files.

    This tutorial describes briefly the tasks to set up a narrow standard calibration and run samples and recalibration standards using a single detector configuration. For further reference go and find details in Chapter 4, “Interactions Between Agilent ChemStation and the Agilent GPC Data Analysis Software and Chapter 5, “Software Windows and Menus. This section requires that you are familiar with operating the standard Agilent ChemStation.

    4 Interactions Between Agilent ChemStation and the Agilent GPC Data Analysis Software

    This chapter describes the how to acquire and process data files starting from the Agilent ChemStation Data Analysis view. The individual tasks and menu items are described in detail.

    5 Software Windows and Menus

    This chapter describes all windows and menu items of the Agilent GPC data analysis software window.

    The menu bar appears on the upper edge of the display regardless of which window is selected. The possible menu options are context specific that means that each active window has its own menu entries. Only the menu item window does not change irrespective of the currently active window.

    6 Appendix

    This chapter provides supplementary information about the software such as system verification.

    7 References

    This chapter gives the literature references quoted in the manual.

    GPC Data Analysis Software for ChemStation 5

  • 6 GPC Data Analysis Software for ChemStation