4.11 To investigate the growth of leaf yeast using agar plates and controls
AGAR PLATES
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Transcript of AGAR PLATES
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AGAR PLATES
*GROUP 1*
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Sterile petri dish containing agar and usually nutrients
Used to culture microorganisms
WHAT ARE AGARS?
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ABOUT AGARSAGARS
REGULAR
“BLOOD”
“SUGAR”
PREPOURED
ADDITIVES
SUSPEND IN H20
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AGAR
DEFINED
Chemicals required
UNDEFINED
Natural products
SELECTIVE
Certain organisms
PERMISSIVE
Many organisms
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Agar plates are used as a medium for growing microorganisms, usually
bacteriaAgar plates contain a gelatin, this is because this substance usually isn't
degraded by bacteria. This is why some plates contain different material
Used to help make decisions with prescribing medications or just for
identificationWHY?
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Commonly used due to high number of
microbes, fungus and bacteria that can be
grown (not selective) Beef broth and
extracts from yeast make it non selective
NUTRIENT AGAR
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Contains 5% blood (sheep)
Can be contaminated
from human contact
Blood Agar
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EMB AGAR
Eosin Methylene Blue
Isolates and differentiation of gram negative Bacilli
One of the many agars that are irritating to the eyes, skin and respiratory system
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Most of agar is made of chocolate,
1% hemoglobin (sheep)
Usually for Haemophilus growth,
fastidious bacteriaCan be
contaminated by human contactCHOCOLATE AGAR
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Usually for growth of gram negative
E.Coli grow into red colonies
AGAR POWDER:Sugar Lactose
Non Sugar
MACCONKEY AGAR
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10g lactose 20g peptone 5g bile salts "pinch" or small amount of 1mg/mL Bromcresol purple or Crystal violet solution. 15g agar1L distilled water
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Kills gram - and +Contains neomycin
antibiotic, which is naturally made from
Streptomyces fradiaeRelatively toxic but
can cause allergic reactions in some
people
NEOMYCIN AGAR
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AGAR TY[ESLB (Luria Bertani) AgarNon-nutrient AgarSabouraud AgarThayer-Martin AgarTryptic Soy AgarXLD Agar Xylose lysine DeoxycholateMannitol salt Potato dextroseMillers LB
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THE END
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