AFM in Biology Workshop - Nano/Bio Interface Center...sponsored by the University of Pennsylvania...
Transcript of AFM in Biology Workshop - Nano/Bio Interface Center...sponsored by the University of Pennsylvania...
New AFM Technology for Biological Applications – Cell Mechanics, High Resolution Imaging and MoreAFM is experiencing an evolution with new techniques and instrumentation for the characterization of biological samples. This informative workshop sponsored by the University of Pennsylvania Nano-BIO Interface Center and Oxford Instruments Asylum Research, will cover topics including cell mechanics, high resolution imaging, combined AFM and optical integration, nano-injection methods and the latest AFM instrumentation innovations. The first day will include lectures from leading AFM scientists in the field. The second day will include hands-on demonstrations relating to the lecture topics. This free workshop is ideal for both novice and experienced AFM users.
Invited SpeakersDennis Discher and Irena Ivanovska, University of Pennsylvania Jan Hoh, Johns Hopkins University Matt Caporizzo, University of Pennsylvania Meg Grady, University of Pennsylvania Biao Han, Drexel University, School of Biomedical Engineering Rob Cain and Sophia Hohlbauch, Oxford Instruments Asylum Research
LocationFeb. 12 Lectures: University of Pennsylvania Singh Center for Nanotechnology Forum Room, 3rd Floor 3205 Walnut St. Philadelphia, PA 19104
Feb. 13 Demos: Room 010, Cypher™ AFM Room 014, MFP-3D-BIO™ AFM Room 020, MFP-3D™ AFM
RegistrationRegistration is free, however, all attendees must register as seating is limited. Registration can be found at www.asylumresearch.com/bioworkshop
ContactsUPenn Matt Brukman, [email protected]
Asylum Research Nushaw Ghofranian, [email protected]
Asylum Research, an Oxford Instruments company
(805) 696-6466 • www.AsylumResearch.com • [email protected]
DNA double helix imaged with Cypher AFM and blueDrive photothermal excitation.
AFM in Biology Workshop
University of Pennsylvania, February 12-13, 2015
AFMAsylum Research
High-resolution force map of an MRC-5 cell imaged with the MFP-3D-BIO AFM.
Feb. 12 Lectures Room
8:30 Registration at Forum Room
9:00 Welcome – Matt Brukman, University of Pennsylvania and Rob Cain, Asylum Research
Session 1: AFM in Biology
9:30 Sophia Hohlbauch, Asylum Research
Introduction to AFM for biologists
10:00 Jan Hoh, Johns Hopkins
Characterization of nanometer scale protein-patterned substrates for cell culture
10:30 Break
Session 2: Instrument Innovations – AFM Made Easier
11:00 Rob Cain, Asylum Research
High resolution imaging and nanomechanics; demonstration
12:00 Lunch
Session 3: Cell Mechanics
1:00 Matt Caporizzo, UPenn
Determining the viscoelasticity of single cells using Variable Indentation Rate Rheology by Laplace Transform, VIRRAL.
1:30 Prathima Nalam, UPenn
Tip based, force modulation AFM using iDrive™: Measuring nano-rheological properties of hydrogels
2:00 Biao Han, Drexel University, School of Biomedical Eng. Science and Health Systems
Time dependent mechanical properties using the MFP-3D AFM
2:30 Break
3:00 Dennis Discher & Irena Ivanovska, UPenn
Matrix to cytoskeleton to nucleus: mechanics of stem cell differentiation
Session 4: Combined AFM/Optical Microscopy and Other Complementary Techniques
3:30 Sophia Hohlbauch, Asylum Research
Combined AFM and optical imaging
4:00 Meg Grady, UPenn
Nano-injection for biological applications
4:30 Concluding remarks
Feb. 13 Demonstrations
9:00-10:30am
Room 014 Elasticity of cells
Room 010 blueDrive: Imaging and nanomechanics
11:00-Noon
Room 014 AFM and optical image overlay
Room 010 blueDrive: Imaging and nanomechanics
1:00-2:30
Room 014 Elasticity of cells
Room 020 Other topic to be announced
AFM in Biology Workshop
University of Pennsylvania, February 12-13, 2015
AFMAsylum Research
Asylum Research, an Oxford Instruments company
(805) 696-6466 • www.AsylumResearch.com • [email protected]