AFM in Biology Workshop - Nano/Bio Interface Center...sponsored by the University of Pennsylvania...

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New AFM Technology for Biological Applications – Cell Mechanics, High Resolution Imaging and More AFM is experiencing an evolution with new techniques and instrumentation for the characterization of biological samples. This informative workshop sponsored by the University of Pennsylvania Nano-BIO Interface Center and Oxford Instruments Asylum Research, will cover topics including cell mechanics, high resolution imaging, combined AFM and optical integration, nano-injection methods and the latest AFM instrumentation innovations. The first day will include lectures from leading AFM scientists in the field. The second day will include hands-on demonstrations relating to the lecture topics. This free workshop is ideal for both novice and experienced AFM users. Invited Speakers Dennis Discher and Irena Ivanovska, University of Pennsylvania Jan Hoh, Johns Hopkins University Matt Caporizzo, University of Pennsylvania Meg Grady, University of Pennsylvania Biao Han, Drexel University, School of Biomedical Engineering Rob Cain and Sophia Hohlbauch, Oxford Instruments Asylum Research Location Feb. 12 Lectures: University of Pennsylvania Singh Center for Nanotechnology Forum Room, 3rd Floor 3205 Walnut St. Philadelphia, PA 19104 Feb. 13 Demos: Room 010, Cypher™ AFM Room 014, MFP-3D-BIO™ AFM Room 020, MFP-3D™ AFM Registration Registration is free, however, all attendees must register as seating is limited. Registration can be found at www.asylumresearch.com/bioworkshop Contacts UPenn Matt Brukman, [email protected] Asylum Research Nushaw Ghofranian, [email protected] Asylum Research, an Oxford Instruments company (805) 696-6466 www.AsylumResearch.com [email protected] DNA double helix imaged with Cypher AFM and blueDrive photothermal excitation. AFM in Biology Workshop University of Pennsylvania, February 12-13, 2015 AFM Asylum Research High-resolution force map of an MRC-5 cell imaged with the MFP-3D-BIO AFM.

Transcript of AFM in Biology Workshop - Nano/Bio Interface Center...sponsored by the University of Pennsylvania...

Page 1: AFM in Biology Workshop - Nano/Bio Interface Center...sponsored by the University of Pennsylvania Nano-BIO Interface Center and Oxford Instruments Asylum Research, will cover topics

New AFM Technology for Biological Applications – Cell Mechanics, High Resolution Imaging and MoreAFM is experiencing an evolution with new techniques and instrumentation for the characterization of biological samples. This informative workshop sponsored by the University of Pennsylvania Nano-BIO Interface Center and Oxford Instruments Asylum Research, will cover topics including cell mechanics, high resolution imaging, combined AFM and optical integration, nano-injection methods and the latest AFM instrumentation innovations. The first day will include lectures from leading AFM scientists in the field. The second day will include hands-on demonstrations relating to the lecture topics. This free workshop is ideal for both novice and experienced AFM users.

Invited SpeakersDennis Discher and Irena Ivanovska, University of Pennsylvania Jan Hoh, Johns Hopkins University Matt Caporizzo, University of Pennsylvania Meg Grady, University of Pennsylvania Biao Han, Drexel University, School of Biomedical Engineering Rob Cain and Sophia Hohlbauch, Oxford Instruments Asylum Research

LocationFeb. 12 Lectures: University of Pennsylvania Singh Center for Nanotechnology Forum Room, 3rd Floor 3205 Walnut St. Philadelphia, PA 19104

Feb. 13 Demos: Room 010, Cypher™ AFM Room 014, MFP-3D-BIO™ AFM Room 020, MFP-3D™ AFM

RegistrationRegistration is free, however, all attendees must register as seating is limited. Registration can be found at www.asylumresearch.com/bioworkshop

ContactsUPenn Matt Brukman, [email protected]

Asylum Research Nushaw Ghofranian, [email protected]

Asylum Research, an Oxford Instruments company

(805) 696-6466 • www.AsylumResearch.com • [email protected]

DNA double helix imaged with Cypher AFM and blueDrive photothermal excitation.

AFM in Biology Workshop

University of Pennsylvania, February 12-13, 2015

AFMAsylum Research

High-resolution force map of an MRC-5 cell imaged with the MFP-3D-BIO AFM.

Page 2: AFM in Biology Workshop - Nano/Bio Interface Center...sponsored by the University of Pennsylvania Nano-BIO Interface Center and Oxford Instruments Asylum Research, will cover topics

Feb. 12 Lectures Room

8:30 Registration at Forum Room

9:00 Welcome – Matt Brukman, University of Pennsylvania and Rob Cain, Asylum Research

Session 1: AFM in Biology

9:30 Sophia Hohlbauch, Asylum Research

Introduction to AFM for biologists

10:00 Jan Hoh, Johns Hopkins

Characterization of nanometer scale protein-patterned substrates for cell culture

10:30 Break

Session 2: Instrument Innovations – AFM Made Easier

11:00 Rob Cain, Asylum Research

High resolution imaging and nanomechanics; demonstration

12:00 Lunch

Session 3: Cell Mechanics

1:00 Matt Caporizzo, UPenn

Determining the viscoelasticity of single cells using Variable Indentation Rate Rheology by Laplace Transform, VIRRAL.

1:30 Prathima Nalam, UPenn

Tip based, force modulation AFM using iDrive™: Measuring nano-rheological properties of hydrogels

2:00 Biao Han, Drexel University, School of Biomedical Eng. Science and Health Systems

Time dependent mechanical properties using the MFP-3D AFM

2:30 Break

3:00 Dennis Discher & Irena Ivanovska, UPenn

Matrix to cytoskeleton to nucleus: mechanics of stem cell differentiation

Session 4: Combined AFM/Optical Microscopy and Other Complementary Techniques

3:30 Sophia Hohlbauch, Asylum Research

Combined AFM and optical imaging

4:00 Meg Grady, UPenn

Nano-injection for biological applications

4:30 Concluding remarks

Feb. 13 Demonstrations

9:00-10:30am

Room 014 Elasticity of cells

Room 010 blueDrive: Imaging and nanomechanics

11:00-Noon

Room 014 AFM and optical image overlay

Room 010 blueDrive: Imaging and nanomechanics

1:00-2:30

Room 014 Elasticity of cells

Room 020 Other topic to be announced

AFM in Biology Workshop

University of Pennsylvania, February 12-13, 2015

AFMAsylum Research

Asylum Research, an Oxford Instruments company

(805) 696-6466 • www.AsylumResearch.com • [email protected]