ÄKTA avant 25 Product...
Transcript of ÄKTA avant 25 Product...
Table of Contents31 Introduction ..........................................................................................................31.1 Instrument view .....................................................................................................................................71.2 Liquid flow path .....................................................................................................................................
122 General specifications .........................................................................................122.1 System specifications .........................................................................................................................122.2 Enviromental requirements .............................................................................................................132.3 Module specifications .........................................................................................................................
193 Material conformity .............................................................................................193.1 Material definitions ..............................................................................................................................203.2 Materials of construction ..................................................................................................................
2 ÄKTA avant 25 Product Documentation 28991000 AH
Table of Contents
1 Introduction
Purpose of this documentThis document provides an overview of ÄKTA avant 25, general specifications and ma-terial conformity. For more information about ÄKTA avant 25, refer to the user documen-tation.
1.1 Instrument view
Front sideThe front side of ÄKTA avant 25 is illustrated below.
Built-in fraction collector
ÄKTA avant 25 Product Documentation 28991000 AH 3
1 Introduction
Standard configuration of thewet side
The standard configuration of ÄKTA avant 25 is illustrated below. See Standardmodules,on page 5 for label descriptions.
V9-pHV9-Inj V9-IBV9-IAC9U9-MU9-DV9-CV9-O
Q9P9 B
FR-902
V9-S
M9
P9-S
R9 (Sample)
P9 A R9 (System)
System pump flow restrictorSample pump flow restrictor
Available modulesÄKTA avant 25 is always delivered with the standard modules installed, but one or moreoptional modules may be added to the flow path. The following tables contain informationon standard modules and optional modules.
4 ÄKTA avant 25 Product Documentation 28991000 AH
1 Introduction1.1 Instrument view
Standard modules
DescriptionStandard module
A high precision pump, which delivers buffer in purification runs.System pump P9 A
A high precision pump, which delivers buffer in purification runs.System pump P9 B
A high precision pump which delivers sample or buffer in purificationruns.
Sample pump P9-S
Reads the system pressure after System pump A, System pump Band Sample pump.
Pressure monitors R9
Prevents the system from siphoning if the flow path after the Systempumps or Sample pump is open.
Pump flow restrictor
Mixes the buffers delivered from the system pumps to a homoge-neous buffer composition. Three mixer chambers are available forÄKTA avant 25 and their volumes are: 0.6 ml, 1.4 ml (mounted atdelivery) and 5 ml.
Mixer M9
Directs sample onto the column.Injection valve V9-Inj
Inlet valve for System pump A with seven inlet ports and integratedair sensor.
Inlet valve A V9-IA
Inlet valve for System pump B with seven inlet ports and integratedair sensor.
Inlet valve B V9-IB
Inlet valve for Sample pump with eight inlet ports (seven sample in-lets and one buffer inlet) and integrated air sensor.
Sample inlet valve V9-IS
Allows automatical mixing of four different solutions.Quaternary valve Q9
Connects up to five columns to the instrument, and directs the flowto one column at a time. The Column valve features two integratedpressure sensors. The valve allows the user to choose flow directionthrough the column, or to bypass the column.
Column valve V9-C
Enables the pH electrode and flow restrictor FR-902 to be includedin the flow path or bypassed during a run. The pH electrode may becalibrated when installed in the pH valve.
pH valve V9-pH
Directs the flow to the Fraction collector, Fraction collector 2 or anyof the ten outlet ports or waste.
Outlet valve V9-O
Measures the UV/Vis absorbance at up to three wavelengths simul-taneously in the range 190-700 nm.
UV monitor U9-M
Detects the UV/Vis absorbance and is connected to U9-M.UV detector U9-D
ÄKTA avant 25 Product Documentation 28991000 AH 5
1 Introduction1.1 Instrument view
DescriptionStandard module
Continuosly measures the conductivity of buffers and sample solu-tions.
Conductivity monitor C9
Built-in flexible fraction collector that can collect up to 576 fractions.A cooling function protects the fractions from heat degradation.
Built-in fraction collector
Optional modules
DescriptionModule
Second inlet valves for System pump A, to extend the number ofinlets up to 14.
Second Inlet valve A V9-A2
Second inlet valves for System pump B, to extend the number of in-lets up to 14.
Second Inlet valve B V9-B2
Inlet valve with eight inlet ports. No integrated air sensor.Extra Inlet valve V9-IX
Second inlet valve for Sample pump to extend the number of sampleinlets up to 14.
Second Sample inlet valveV9-S2
A 4-port, 4-position valve, which can be used to customize the flowpath.
Versatile valve V9-V
Enables the use of up to five loops connected to the instrument.Loop valve V9-L
Valve which connects five additional columns to the instrument,extending the number of columns up to 10. The valve allows theuser to choose flow direction through the column, or to bypass thecolumn.
Second Column valve V9-C2
Valve which adds 12 outlet ports to the system, giving a total of 21outlets.
Second Outlet valve V9-O2
Valve which adds 12 outlet ports to the system, giving a total of 32outlets.
Third Outlet valve V9-O3
Sensor which prevents air from being introduced into the flow path.External air sensor L9-1.5 or L9-1.2
Module which receives analog or digital signals from, or transfersanalog or digital signals to, external equipment that has been con-nected to the system.
I/O-box E9
Monitor which measures the UV absorbance at a fixed wavelengthof 280 nm.
Second UV monitor U9-L
6 ÄKTA avant 25 Product Documentation 28991000 AH
1 Introduction1.1 Instrument view
DescriptionModule
Monitor which measures the conductivity of buffers and samplesolutions.
Second Conductivity monitorC9
Round fraction collector that can collect up to 175 fractions.Second Fraction collector F9-R
1.2 Liquid flow path
Illustration of tubingThe liquid flow path and system functionality can be extended in multiple ways to fit theuser's needs. One or more optional components can be added to the flow path. Externalequipment can also be connected to the instrument via the I/O-box E9.
The illustration shows a detailed flow chart for ÄKTA avant 25. The flow chart showsconnected tubing between instrument components.
ÄKTA avant 25 Product Documentation 28991000 AH 7
1 Introduction1.1 Instrument view
Inlet tubingThe table below shows the labels, diameters, and standard lengths of the inlet tubing.The table also shows which tubing is mounted on delivery.
MountedLength(mm)
TubingDescriptionLabel
A1 and A2mounted
1500FEP, o.d. 1/8″, i.d. 1.6 mmInlets to Inlet valve AA1-A7
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1 Introduction1.2 Liquid flow path
MountedLength(mm)
TubingDescriptionLabel
B1 and B2mounted
1500FEP, o.d. 1/8″, i.d. 1.6 mmInlets to Inlet valve BB1-B7
Yes1500FEP, o.d. 1/8″, i.d. 1.6 mmInlets to Quaternary valveQ1-Q4
Yes1000FEP, o.d. 1/8″, i.d. 1.6 mmBuffer inlet to Sample inletvalve
Buff
S1 and S2mounted
700FEP, o.d. 1/8″, i.d. 1.6 mmInlets to Sample inlet valveS1-S7
Yes110FEP, o.d. 1/8″, i.d. 1.6 mmFrom Quaternary valve toInlet valve A
InQA
Yes110FEP, o.d. 1/8″, i.d. 1.6 mmFrom Quaternary valve toInlet valve B
InQB
Yes220FEP, o.d. 1/8″, i.d. 1.6 mmFrom Inlet valve A to Sys-tem pump A
InA
Yes220FEP, o.d. 1/8″, i.d. 1.6 mmFrom Inlet valve B to Sys-tem pump B
InB
Yes220FEP, o.d. 1/8″, i.d. 1.6 mmFrom Sample inlet valve Bto Sample pump
InS
High pressure tubingThe table below shows the labels, diameters, and standard lengths of the high pressuretubing. The high pressure tubing is mounted on delivery.
MountedLength(mm)
TubingDescriptionLabel
Yes340PEEK, o.d. 1/16″, i.d. 0.75 mmSystem pump A left toRestrictor A
1A1
Yes340PEEK, o.d. 1/16″, i.d. 0.75 mmSystem pump A right toRestrictor A
1A2
Yes100PEEK, o.d. 1/16″, i.d. 0.75 mmRestrictor A to Pressuremonitor
2A
Yes340PEEK, o.d. 1/16″, i.d. 0.75 mmSystem pump B left toRestrictor B
1B1
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1 Introduction1.2 Liquid flow path
MountedLength(mm)
TubingDescriptionLabel
Yes340PEEK, o.d. 1/16″, i.d. 0.75 mmSystem pump B right toRestrictor B
1B2
Yes100PEEK, o.d. 1/16″, i.d. 0.75 mmRestrictor B to Pressuremonitor
2B
Yes340PEEK, o.d. 1/16″, i.d. 0.75 mmSample pump left to Re-strictor S
1S1
Yes340PEEK, o.d. 1/16″, i.d. 0.75 mmSample pump right toRestrictor S
1S2
Yes100PEEK, o.d. 1/16″, i.d. 0.75 mmRestrictor S to Pressuremonitor
2S
Yes280PEEK, o.d. 1/16″, i.d. 0.75 mmPressure monitor to Mixer3
Yes485PEEK, o.d. 1/16″, i.d. 0.75 mmPressure monitor S to In-jection valve
3S
Yes400PEEK, o.d. 1/16″, i.d. 0.75 mmMixer to Injection valve4
Yes180PEEK, o.d. 1/16″, i.d. 0.50 mmInjection valve to Columnvalve
5
Yes160PEEK, o.d. 1/16″, i.d. 0.50 mmColumn valve to UVmonitor
6
Yes100PEEK, o.d. 1/16″, i.d. 0.50 mmUV monitor to Conductiv-ity monitor
7
Yes165PEEK, o.d. 1/16″, i.d. 0.50 mmConductivity monitor topH valve
8
Yes215PEEK, o.d. 1/16″, i.d. 0.50 mmpH valve to Outlet valve9
Yes75PEEK, o.d. 1/16″, i.d. 0.50 mmTo Flow restrictor1R
Yes75PEEK, o.d. 1/16″, i.d. 0.50 mmFrom Flow restrictor2R
Yes1280PEEK, o.d. 1/16″, i.d. 0.50 mmOutlet valve to Fractioncollector
Frac
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1 Introduction1.2 Liquid flow path
Outlet tubingThe table below shows the labels, diameters, and standard lengths of the outlet tubing.
MountedLength(mm)
TubingDescriptionLabel
No1000ETFE o.d. 1/16″, i.d. 1.0 mmOutlets from Outlet valveOut1 -Out32
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1 Introduction1.2 Liquid flow path
2 General specifications
2.1 System specificationsDataParameter
Benchtop system, external computerSystem configuration
UNICORN™ 6.0 or later versionControl system
EthernetConnection between PC and instrument
860 x 710 x 660 mmDimensions (Length x Depth x Height)
116 kgWeight (excluding computer)
100-240 VAC, 50-60 HzPower supply
800 VAPower consumption
IP 21, wet side IP 22Enclosure protective class
• Inlet: FEP tubing, inner diameter (i.d.) 1.6 mm,Tubing connector 5/16″ + Ferrule (yellow), 1/8″
• Pump to Injection valve: PEEK tubing, i.d.0.75 mm, Fingertight connector, 1/16″
• After Injection valve: PEEK tubing, i.d. 0.50 mm,Fingertight connector, 1/16″
• Outlet and waste: ETFE tubing, i.d. 1.0 mm,Fingertight connector, 1/16″
Tubing and connectors
2.2 Enviromental requirementsDataParameter
-25°C to 60°CStorage and transport temperature range
4°C to 35°COperating temperature range
20% to 95%, non-condensingRelative humidity
See ÄKTA avant Operating Instructions.Chemical environment
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2 General specifications
2.3 Module specifications
System pumps
DataParameter
Piston pump, metering typePump type
0.001 to 25 ml/min (normal range)Flow rate range
0.01 to 50 ml/min (column packing flow)
0 to 20 MPa (0 to 200 bar)Pressure range
0.35 to 10 cPViscosity range
• Accuracy: ±1.2%
• Precision: RSD < 0.5%
(Conditions: 0.25 to 25 ml/min, < 3 MPa, 0.8 to 2 cP)
Flow rate specifications
Sample pump
DataParameter
Piston pump, metering typePump type
0.001 to 50 ml/minFlow rate range
0 to 10 MPa (0 to 100 bar)Pressure range
0.7 to 10 cPViscosity range
• Accuracy: ±2%
• Precision: RSD < 0.5%
(Conditions: 0.25 to 50 ml/min, < 3 MPa, 0.8 to 3 cP)
Flow rate specifications
Mixer
DataParameter
Chamber with a magnetic stirrerMixing principle
0.6 ml, 1.4 ml (default) or 5 mlMixer volume
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2 General specifications2.3 Module specifications
Gradient formation
DataParameter
Binary: 0.25 to 25 ml/minGradient flow rate range
Quaternary: 1 to 25 ml/min
• Binary: ±0.6%
• Quaternary: ±1%
(Conditions: 5 to 95 B. 0.5 to 25 ml/min, 0.2 to 2 MPa, 0.8 to2 cP)
Gradient composition accuracy
Valves
DataParameter
Rotary valvesType
6 standard valves, up to 6 optional valvesNumber of valves
Inlet valve, Sample inlet valve , Injection valve, Column valve,pH valve, Outlet valve
Functions
Second Inlet valve, Loop selection valve, Versatile valve,Second Column valve, Extra Outlet valve, Extra Inlet valve
Options
Quaternary valve
DataParameter
4-port solenoid actuated membrane valveType
Quaternary gradients or BufferProFunctions
Number of inlets
DataParameter
7, expandable to 14Inlet A
7, expandable to 14Inlet B
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2 General specifications2.3 Module specifications
DataParameter
7, expandable to 14Sample inlet
4Quaternary inlet
Pressure monitors
DescriptionItem
4Number of sensors
After System pump, after Sample pump, integrated in columnvalve (pre-column and post-column)
Placement of sensors
0 to 20 MPa (0 to 200 bar)Range
±0.02 MPa or ±2% whichever is greaterAccuracy
Air sensors
DescriptionItem
Integrated in Inlet A, Inlet B and Sample inletPlacement of sensors
Before sample inlet valve, after injection valveOptional external placement
UltrasonicSensing principle
UV monitors
DescriptionItem
Up to 2Number of monitors
U9-M: 190 to 700 nm in steps of 1 nm, up to 3 wavelengthsWavelength range
U9-L: 280 nm
-6 to 6 AUAbsorbance range
U9-M: within ± 2% at 0 to 2 AULinearity
U9-L: within ± 5% at 0 to 2 AU
ÄKTA avant 25 Product Documentation 28991000 AH 15
2 General specifications2.3 Module specifications
DescriptionItem
U9-M (2 mm cell at 280 nm): ≤ | 0.2 mAU | AU/hDrift
U9-L (2 mm cell): ≤ | 0.2 mAU | AU/h
U9-M: < 0.08 mAUNoise
U9-L: < 0.1 mAU
0 to 2 MPaOperating pressure
Standard:Optical path length 2 mmCell volume 2 µlTotal volume: 11 µl
Flow cells: U9-M
Option:Optical path length 10 mmCell volume 8 µlTotal volume 12 µl
Optical path length 0.5 mmCell volume 1 µlTotal volume 10 µl
Standard:Optical path length 2 mmCell volume 2 µlTotal volume: 30 µl
Flow cells: U9-L
Option:Optical path length 5 mmCell volume 6 µlTotal volume 20 µl
Conductivity monitor
DescriptionItem
Up to 2Number of monitors
0.01 to 999.99 mS/cmConductivity reading range
±0.01 mS/cm or ±2%, whichever is greater,
(within 0.3 to 300 mS/cm)
Accuracy
0 to 5 MPa (0 to 50 bar)Operating pressure
22 µlFlow cell volume
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2 General specifications2.3 Module specifications
Temperaturemonitor integratedin Conductivity monitor
DescriptionItem
0°C to 99°CReading range
±1.5°C within 4°C to 45°CAccuracy
pH monitor
DescriptionItem
0 to 14pH reading range
±0.1 pH unitAccuracy
(within pH 2 to 12, temp. within 3°C from calibration temp.)
0 to 0.5 MPa (0 to 5 bar)Operating pressure
76 µlFlow cell volume
Outlet valve fractionation
DescriptionItem
10, expandable to 32Number of outlets
0.1 to 20000 mlFraction volumes
142 µl (with pH electrode and flow restrictor off-line)Delay volume (UV – Outlet valve)
Built-in fraction collector
DescriptionItem
Up to 576Number of fractions
• 3, 5, 8, 15, 50 ml tubes
• 250 ml bottles
• Deep well plates: 96 / 48 / 24
Vessel types
ÄKTA avant 25 Product Documentation 28991000 AH 17
2 General specifications2.3 Module specifications
DescriptionItem
Automatic recognitionVessel type selection
0.1 to 250 mlFraction volumes
Automatic, DropSync or accumulatorSpillage-free modes
Covered vessels and climate control (settable 6°C to 20°C)Protection of fractions
NoOrganic solvents
518 µl (with pH electrode and flow restrictor off-line)Delay volume (UV – dispenser head)
Fraction collector F9-R, 2nd
DataParameter
Up to 175Number of fractions
3 ml, 8 ml, 15 ml or 50 ml tubesVessel types
0.1 to 50 mlFraction volumes
DropSyncSpillage-free mode
YesFractionate flammable liquids
240 µl (with pH electrode and flow restrictor off-line)Delay volume (UV – dispenser head)
320 x 400 x 250 mmDimensions (W x D x H)
5 kgWeight
I/O box
DataParameter
2 analog in, 2 analog out4 digital in, 4 digital out
Number of ports
In +/- 2 VOut +/- 1 V
Analog range
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2 General specifications2.3 Module specifications
3 Material conformity
3.1 Material definitions
IntroductionThe tables below list the primary wetted materials in the flow path and the pump rinsingsystem of the ÄKTA avant 25 system.
Primary flow path
AbbreviationMaterial
ECTFEEthylene ChloroTriFluoroEthylene
ETFEEthylene TetraFluoroEthylene
FEPFluorinated Ethylene Propylene
FPM/FKMFluorinated Propylene Monomer
FFPM/FFKMFully Fluorinated Propylene Monomer
PCTFEPolyChloroTriFluoroEthylene
PEEKPolyEtherEtherKetone
PPPolyPropylene
PTFEPolyTetraFluoroEthylene
UHMWPEUltraHighMolecularWeightPolyEthylene
AluminaAluminum oxide
Elgiloy
Hastelloy™ C-276
Quartz glass
Ruby
Sapphire
Titanium grade 2
ÄKTA avant 25 Product Documentation 28991000 AH 19
3 Material conformity
AbbreviationMaterial
Titanium grade 5 1
1 Used in pressure sensors only.
Pump rinse system
AbbreviationMaterial
EPDMEthylenePropyleneDiene M-class rubber
PEEKPolyEtherEtherKetone
PPPolyPropylene
PPSPolyPhenylene Sulfide
PVDFPolyVinylidene DiFluoride
Silicone
3.2 Materials of construction
IntroductionThe following tables list the materials used in flow path and pump rinse system compo-nents.
Primary flow path
MaterialComponentCode No.Part
PEEKPEEKPEEK/EPDMPEEK/EPDMPEEK/EPDMPEEK/EPDM
28924653 Q9 Quaternary valve28944270 Buffer Prep Mixing Housing28929467 Q9 P2-1 Mixing Plug28924596 Solenoid valve 2/2 type 6606, Marking Q428924595 Solenoid valve 2/2 type 6606, Marking Q328924592 Solenoid valve 2/2 type 6606, Marking Q228924589 Solenoid valve 2/2 type 6606, Marking Q1
28950503Q9
20 ÄKTA avant 25 Product Documentation 28991000 AH
3 Material conformity3.1 Material definitions
MaterialComponentCode No.Part
SapphireECTFEEPDM
TitaniumPEEKUHMWPE/Elgiloy
28942298 Pump P9 Cpl (primary flow path)56116124 Piston28945400 Y-Connector56119415 Membrane28939480 Pump Head P920939097 Pump Head28943626 Purge Valve56118261 Seal
-P9 AP9 B
PEEKPEEKPEEKSapphire/Ruby
PEEKPEEKSapphire/RubyPEEK
Check valves in/out28963058 Outlet Check valve28962655 Valve housing Out28962657 Ball retainer28962659 Washer28950137 Ball and Seat28963062 Inlet Check Valve28962653 Valve housing In28962657 Ball Retainer28950137 Ball and Seat56305879 Purge Valve
AluminaECTFEEPDM
TitaniumPEEKUHMWPE/Elgiloy
28945183 Pump P9 Cpl (primary flow path)28945183 Piston56117787 Y-Connector28978573 Membrane28952471 Pump Head P9-S56305641 Pump Head28943626 Purge Valve28962521 Seal
Check valves in/out28963058 Outlet Check valve28962655 Valve housing Out28962657 Ball retainer28962659 Washer28950137 Ball and Seat28963062 Inlet Check Valve28962653 Valve housing In28962657 Ball Retainer28950137 Ball and Seat56305879 Purge Valve
-P9-S
PEEKPEEKPEEKSapphire/Ruby
PEEKPEEKSapphire/RubyPEEK
ÄKTA avant 25 Product Documentation 28991000 AH 21
3 Material conformity3.2 Materials of construction
MaterialComponentCode No.Part
PEEKTitanium
Hastelloy C-276FFPM/FFKMPEEKPEEKPEEK
28944995 Pressuremonitor R9 (System)with pumpflow restrictor28951451 Pressure monitor R9 (System)28947686 Pressure connector28933525 Pressure sensor28945164 Restrictor Housing R9 (System) Assembly28977560 Compression Spring28966920 Membrane28989942 Plunger28946870 Restrictor Stopper28946577 Pump Restriction Housing
-R9(Systempumps)
PEEKTitanium
Hastelloy C-276FFPM/FFKMPEEKPEEKPEEK
28944998 Pressuremonitor R9 (Sample) with pumpflow restrictor28951453 Pressure monitor R9 (Sample)28947688 Pressure connector28933525 Pressure sensor28945174 Restrictor Housing R9 (Sample) Assembly28977560 Compression Spring28966920 Membrane28989942 Plunger28946870 Restrictor Stopper28947779 Pump Restriction Housing
-R9(Samplepump)
PPPPPEEKPTFEPEEKFPM/FKM
28922334 Mixer chamber 0.6 ml56302238 Filter 10PP (1 µm)56302237 Support net28945536 Mixer top28963112 Stirring magnet 9.1 mm28916429 Mixer chamber 0.6 ml28945544 O-ring 13.1 x 1.6
28956186M9-0.6
PPPPPEEKPTFEPEEKFPM/FKM
28924642 Mixer chamber 1.4 ml56302238 Filter 10PP (1 µm)56302237 Support net28945536 Mixer top28924648 Stirring magnet 12 mm28924646 Mixer chamber 1.4 ml28945544 O-ring 13.1 x 1.6
28956225M9-1.4
22 ÄKTA avant 25 Product Documentation 28991000 AH
3 Material conformity3.2 Materials of construction
MaterialComponentCode No.Part
PPPPPEEKPTFEPEEKFPM/FKM
28924700 Mixer chamber 5 ml56302238 FILTER 10PP (1 µm)56302237 Support net28945536 Mixer top56105749 Stirring magnet 12 mm28924702 Mixer chamber 5 ml28945544 O-ring 13.1 x 1.6
28956246M9-5
FFKM28948433 O-ring 13.1 x 1.6 mm High resistant29011326
PEEKPEEK/PTFE
28920910 Injection valve V9-Inj28943034 Valve stator injection28943040 Valve rotor injection
-V9-Inj
PEEKFFPM/FFKM
56304545 Flow restrictor FR-90256302557 Housing56303929 Diaphragm
18112135FR-902
PEEKPEEKPEEK
Sample Inlet Valve (V9-IS, 7 ports)28920915 Sample Inlet Valve V9-IS28934791 Valve stator inlet 1.5 assembly28934276 Valve stator inlet 1.528934290 Valve rotor inlet28934287 Valve inlet plug
28962007V9-IS
PEEKPEEK/PTFE
28992313 Versatile valve V9-V28987417 Stator versatile valve28987420 Valve rotor versatile
29011353V9-V
PEEKPEEK/PTFEPEEKPEEK
Loop valve kit (V9-L)28987182 Stator Loop Valve28924597 Valve rotor column29011637 Tubing L129011638 Tubing L2
29011358V9-L
L1L2
PEEKPEEK/PTFE
Outlet Valve (V9-O, 10 outlets)28920867 Valve stator out28933172 Valve rotor out
28956512V9-O
PEEKPEEKPEEK
Inlet valve V9-IA28934791 Valve stator inlet 1.5 assembly28934287 Valve inlet plug28934276 Valve stator inlet 1.528934290 Valve rotor inlet 1.5
28956510V9-IA
ÄKTA avant 25 Product Documentation 28991000 AH 23
3 Material conformity3.2 Materials of construction
MaterialComponentCode No.Part
PEEKPEEKPEEK
Inlet valve V9-IB28934791 Valve stator inlet 1.5 assembly28934287 Valve inlet plug28934276 Valve stator inlet 1.528934290 Valve rotor inlet 1.5
28962006V9-IB
PEEKPEEK/PTFE
PTFEFFPM/FFKM
pH valve V9-pH28939643 Valve stator pH28939641 Valve rotor pH56322802 Dummy pH56119556 pH Electrode dummy56119557 O-ring 5.3 x 2.4
28956508V9-pH
PEEK/PTFE
PEEKPEEK
Column valve V9-C28924597 Valve rotor column28920925 Valve stator column assembly28931925 Valve stator column 2.028920897 Valve column plug
28956506V9-C
PEEK
PPPEEKPEEK
BorosilicateEPDM
PEPPSPEEK
28915000 Fraction collector F956119406 Tubing i.d. 0.5 mm, o.d. 1/16"28915100 SLED ARM cpl28922162 Capillary connector28926764 Fitting UNF 10-32 ferrule28947864 CAPILLARY / Accumulator28915400 Accumulator sled cpl28921813 Glass tube Ø10 / 4428921297 O-ring 10 x 1,5 EPDM 7028902730 Piston Ø 1028930043 Drop sync28949863 Main nozzle part asm28946078 Main nozzle part28949866 Plug, nozzle
-Built-infractioncollector
PEEKFraction collector F9-R
56119406 Tubing i.d. 0.5 mm, o.d. 1/16"29011362F9-R
PEEKTitaniumPCTFE
Conductivity monitor C928921084 Thread housing28902003 Electrode28902005 Insulator
28956495C9
PEEKTitaniumPCTFE
Second Conductivity monitor28921084 Thread housing28902003 Electrode28902005 Insulator
29011363C9
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3 Material conformity3.2 Materials of construction
MaterialComponentCode No.Part
PEEKPEEKQuartz glassPEEK
PEEKPEEK
28975936 UV flow cell 2 mm for U9-M28975932 Cell In 1000 assembly28975442 Cell In 100028975447 Cone 100028977556 UV Fiber 100028975445 Cell Shims 2.0 100028975934 Cell Out 2.0 assembly56001792 Cone 40028975444 Cell Out 2.0
28979380U9-2
(All sizesof flowcells forU9-Mcontainsthesewettedmateri-als)
TitaniumTitaniumQuartz glass
PTFE
56305582 UV Cell 2 mm for U9-L56305584 Cuvette56305586 Fix bushing56068200 Cuvette ANS. 2 U56068800 Seal assembly56068900 Seal
29011325U9-L
(All sizesof flowcells forU9-Lcon-tainsthesewettedmateri-als)
PEEK56118577 Fingertight HPLC-
ÄKTA avant 25 Product Documentation 28991000 AH 25
3 Material conformity3.2 Materials of construction
MaterialComponentCode No.Part
28930248 Capillary and Tubing kit28956606
56303131 Sample loop 500 µl
PEEK56118577 Fingertight HPLC
PEEK56303343 Loop 500 µL Bearb.
FEP28923732 Tubing A1A1
FEP28923987 Tubing A2A2
FEP28923993 Tubing B1B1
FEP28923994 Tubing B2B2
FEP28924000 Tubing Q1Q1
FEP28924001 Tubing Q2Q2
FEP28924002 Tubing Q3Q3
FEP28924003 Tubing Q4Q4
FEP28924004 Tubing S1S1
FEP28924005 Tubing S2S2
FEP28924011 Tubing BuffBuff
FEP28924012 Tubing lnQAlnQA
FEP28924013 Tubing lnQBlnQB
FEP28924014 Tubing lnAlnA
FEP28924015 Tubing lnBlnB
FEP28924016 Tubing lnSlnS
PEEK28924353 Tubing 99
PEEK28924354 Tubing 88
PEEK28924355 Tubing 2R2R
PEEK28924356 Tubing 1R1R
PEEK28924357 Tubing 77
PEEK28924358 Tubing 66
PEEK28924359 Tubing 55
PEEK28924371 Tubing 1A11A1
PEEK28924374 Tubing 1A21A2
PEEK28924375 Tubing 1B11B1
PEEK28924376 Tubing 1B21B2
PEEK28924377 Tubing 1S11S1
PEEK28924378 Tubing 1S21S2
PEEK28924380 Tubing 2S2S
26 ÄKTA avant 25 Product Documentation 28991000 AH
3 Material conformity3.2 Materials of construction
MaterialComponentCode No.Part
3 PEEK28924381 Tubing 3
W1 ETFE28924392 Tubing W1
W2 ETFE28924394 Tubing W2
W3 ETFE28924395 Tubing W3
W ETFE28924396 Tubing W
2A PEEK28955484 Tubing 2A
2B PEEK28955485 Tubing 2B
3S PEEK28955488 Tubing 3S
4 PEEK28955489 Tubing 4
Frac PEEK28955627 Tubing Frac
Pump rinse system
MaterialComponentCode No.Part
SiliconeTubing80100651
Silicone59129200 Tube i.d. 2.1 mm, o.d. 4.1 mm-
PP28959057 BD Falcon™ 50 ml tube-
PVDF/PEEK/AluminaPVDFPPSPPSEPDM
28942298 Pump P9 Cpl (rinse system)29140541 Drainage Check Valve IN29140537 Drainage Check Valve Housing28959717 Pump Wash Housing28959720 Pump Drainage Plate28950435 Membrane 25 ml
28953655P9 AP9 B
PVDF/PEEK/AluminaPVDFPPSPPSEPDMPEEK
28945183 Pump P9-S Cpl (rinse system)29140541 Drainage Check Valve IN29140537 Drainage Check Valve Housing28977775 Pump Wash House56116470 Pump Drainage Plate28978573 Membrane 100 mlUnion, Luer to M6
18111203P9-S
ÄKTA avant 25 Product Documentation 28991000 AH 27
3 Material conformity3.2 Materials of construction
Material conformity: SignatureThe Quality System of GE Healthcare is certified according to ISO9001, and is thereby incontrol of the product realization process. GE Healthcare has a controlled process forquality assurance in selection, assessment and evaluation of supplier where strict adher-ence to specifications for all material is the basis.
Valid from 14 November 2012Thomas WallinQA Site LeaderGE Healthcare
28 ÄKTA avant 25 Product Documentation 28991000 AH
3 Material conformity3.2 Materials of construction
GE and GE monogram are trademarks of General Electric Company.
ÄKTA and UNICORN are trademarks of General Electric Company or one of itssubsidiaries.
Hastelloy is a trademark of Haynes International Inc.
Falcon is a trademark of Becton, Dickinson and Company.
All other third party trademarks are the property of their own trademarks.
© 2013-2015 General Electric Company – All rights reserved.First published May 2013
All goods and services are sold subject to the terms and conditions of sale ofthe company within GE Healthcare which supplies them. A copy of these termsand conditions is available on request. Contact your local GE Healthcare repre-sentative for the most current information.
GE Healthcare Europe GmbHMunzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare UK LimitedAmersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK
GE Healthcare Bio-Sciences Corp.800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, USA
GE Healthcare Japan CorporationSanken Bldg. 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073, Japan
For local office contact information, visitwww.gelifesciences.com/contact
GE Healthcare Bio-Sciences ABBjörkgatan 30751 84 UppsalaSweden
www.gelifesciences.com/avant
28991000 AH 03/2015 a44