Advisor: Wei-Shou Hu Speaker: Justice Akhuamhenkhun Ace Lai PRODUCTION OF 1,3-PROPANEDIOL THROUGH...
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Transcript of Advisor: Wei-Shou Hu Speaker: Justice Akhuamhenkhun Ace Lai PRODUCTION OF 1,3-PROPANEDIOL THROUGH...
Advisor: Wei-Shou Hu
Speaker: Justice Akhuamhenkhun Ace Lai
PRODUCTION OF 1,3-PROPANEDIOL THROUGH METABOLIC ENGINEERING
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Properties of 1,3-PD
• Molecular weight 76.09
• Viscous and colorless liquid
• Miscible with water
• Density = 1.0597 g/ cm
• MP = -28; BP, between 210 to 212 (celsius degree)
• Unsaturated compound with hydroxyl functional group
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Applications
• copolyesters, polymers, textile fibres, and carpets
• organic solvent ingredient of wood paints and anti-freeze
• chief ingredient manufacturing adhesives, laminates, terracota- as protective reagent
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Why we use bioengineering
• Cheaper in cost
• Easier in designing pathway
• Greener to environment
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Metabolic pathway
Metabolic engineering I
• nature of synthesizing of 1,3PD
• tpi gene
• dhaT and dhaB, gene encoding glycerol dehydratase and PDOR
• DAR1, GPP2 encoding 3-
phosphate dehydrogenase and glycerol 3-phosphate phosphatase
Metabolic engineering II
• Redesigning of yqhD
• Use of NADPH instead NADH
• knock down of tpi to manipulate pathway more efficiently
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Calculation analysis
• Yield, 1,3-propanediol at a rate of 3.5 g/L/h, a titer of 135 g/L, and a weight yield of 51% in D-glucosefed-batch 10 L fermentations.
9Conclusion• DAR1 and GPP2 cloned from
Saccharamyces cerevisiae encoding 3-phosphate dehydrogenase
• dhaB, dhaT gene from Klebsiella pneumoniae encoding PDOR and glycerol dehydratase
• yqhD from E. coli can produce PDOR as well
• tpi manipulated via knockdown to resolve carbon flux
• PEP and ATP dependent glucose pathway modified
• In all, contribute to higher yield synthesis