Advances in breeding bean for drought tolerance and canning quality in Kenya

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Presented by Paul Kimani, R.D. Narla, A. Warsame, B. Buxton, P.K. Waidhima, M. Njiru and J.W. Mwangi at the First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013

Transcript of Advances in breeding bean for drought tolerance and canning quality in Kenya

  • 1. Advances in Breeding bean for Drought tolerance and Canning quality in KenyaPaul Kimani1, R.D. Narla1, A. Warsame1, B. Buxton1, P. K.Waidhima2, M. Njiru2 and J.W. Mwangi11Department of Plant Science and Crop Protection,College of Agriculture and Veterinary Sciences, University of Nairobi 2 Trufoods Ltd Nairobi, Kenya First Bio-Innovate Regional Scientific ConferenceUnited Nations Conference Centre (UNCC-ECA) Addis Ababa, Ethiopia, 25-27 February 2013

2. ContentsDrought tolerance Mesoamerican AndeanMechanisms of drought tolerance Shoot traits Root traitsParticipatory selection for drought toleranceCanning QualityPartnership for Seed production and DisseminationConclusions and future directionsAcknowledgements 3. Rationale for Breeding Canning Beans Adapted to Biotic and Abiotic stressesProcess Current variety (Mex 142) more than 50 years old, susceptible to biotic and abiotic stresses445 lines Many farmers stopped growing the varietyAgronomic Processing industry not able to meet demandpotential- yield, e.g. 300 t /year for Trufoodsdiseases, vigour Erratic supply and poor quality raw materialsgrowth habit Industry demanded better varieties Demand for fast cooking or pre-cooked products high energy costs150 lines Changing eating habits -wider range ofDrought preferences with urbanizationtolerance & farmer Focus on 7 market classes for canning industry preferences Fast cooking- for direct consumptionCanning Pre-cooked productsand Canned products20-25 lines Nutritionalquality 3 4. Gene pools and Market ClassesAndeanMesoamericanRed Small Navymottled Red Pinto CariocaRedkidneyTan Yellow Speckled sugarGreen yellow BEIGE4 5. Structure of Breeding ProgramThree parallel Breeding Streams:Stream 1: Advanced lines of a genetically diverse lines Objective: drought tolerance, yield potential andcanning quality 447 Mesoamerican and Andean linesStream 2: Biofortified varieties combining high iron(>90 ppm) and zinc (>35 ppm) multiple stress to biotic and biotic stresses Based on 47 new populations segregating for multiple traitsStream 3: Marker assisted gamete selection for simultaneous selection for resistance to biotic and abiotic stress factors, marketable grain types Focus markers and diseases: anthracnose, angular leaf spot, root rots, common bacterial blight, bean common mosaic virus5 6. New drought tolerant Mesoamerican lines 2000 DMC11-10Mean=903.9DNB11-07 1800 LSD=148.3New DroughtGrain yield in kg ha-1 under no stressDNB11-06 tolerant Navy, 1600 DNB11-15DMC11-01DSR11-24 Small Red andDMC11-24 1400DSR11-13Mixed colour MEX142 DSR11-20lines are better 1200 GLPX92DSR11-19yielding than 1000DSR11-05TIO CANELA local and international 800 DMC11-15 GLP585 checks inDMC11-08 600KATB1drought Mean=753.2 400 LDS=148.3 stressed and400600 8001000 12001400 1600 non-stressed Grain yield in kg ha-1 under drought stress conditions 7. New drought tolerant Andean lines 900 New Drought tolerant 800DSS 11-04DRM 11-03 Red mottled, Red kidneys DSS 11-17 Speckled sugarDrought stress yield (kg ha-1) 700DRK 11-05DRK 11-22DRM 11-17 lines are better 600 GLP 1004 11-01DPCDRK 11-19KAT 56 DRK 11-15 DSS 11-16 11-10DRK DRK 11-18 yielding thanDPC 11-03Kabete Super 11-16 DRK 500DSS 11-01 11-21 DRK local checks inDRK 11-23 Super RosococoDRM 11-15KSBDRM 11-18DRM 11-13 drought stressed 400 GLP 24 GLP 92 KRK KAT 69 and non-stressedMiezi Mbili conditions at 300 GLP 2 Kabete and Kenya Umoja Kenya Wonder New RosococoMwea 200Kenya Early 100 400 500600 700800900 10001100 1200Reduced stress yield (kg ha-1) RS vs DS RS vs DS DRM 11-03 vs DS: 859.45 RS 8. Mechanisms of Drought tolerance- Shoot traits Plant traitsIrrigated Rainfed (Stress) Canopy biomass (kg/ha)0.64***0.25** Pod harvest index (%) 0.62*** 0.40*** Grain harvest index (%) 0.50*** 0.39*** Pod partitioning index (%)0.57*** 0.89*** Pod wall biomass proportion (%)0.26**0.19* Stem biomass reduction (%) -0.18*0.32** Total chlorophyll content (SPAD) 0.24**0.18***, **, *** Significant at p