Advancement in Cell culture Techniques 2000 onward
Transcript of Advancement in Cell culture Techniques 2000 onward
Zoology Department , Govt.College University Lahore
Course Title: Animal Cell & Tissue Culture
Course Code: Z-7105
Advances In Animal Cell Culture
Techniques After 2000
Presented By : Sarwar A.D
Keril et al.,
2000
They established a robust, cell-based system for
genetic and functional analyses of HCV
replication in cell culture.
Kwon et al.,
2000
They developed the techniques of Rapid cell
sheet detachment from Poly (N-
isopropylacrylamide)-grafted porous cell
culture membranes (PIPAAm)
Benjamin et
al., 2000
They described the derivation of pluripotent
embryonic stem (ES) cells from human
blastocysts for somatic differentiation in viro.
Major Research In Animal Cell Culture in 2000
Edna et al.,
2001
characterized the composition and function of
adhesions in three-dimensional (3D) matrices
derived from tissues or cell culture.
Nicole et al.,
2001
Enhancement of Hepatitis C Virus RNA
Replication by Cell Culture-Adaptive
Mutations
Annie Sittler et
al.,2001
This is the first demonstration that huntingtin
protein aggregation in cells can be suppressed
by chemical compounds activating a specific
heat shock response.
Chu &
Robinson.,
2001
Industrial choices for protein production by large-
scale cell culture using standard stirred tank
reactors
Potter &
DeMarse.,
2001
They developed a new method for culturing cells
that maintains their health and sterility for many
months for studies
Keefer et al.,
2001
Production of Cloned Goats after Nuclear
Transfer Using Adult Somatic Cells- These results
demonstrated that adult caprine somatic cells
could direct normal development after Nuclear
Transfer.
Shimizu et
al., 2002
Fabrication of Pulsatile Cardiac Tissue Grafts
Using a Novel 3-Dimensional Cell Sheet
Manipulation Technique and Temperature-
Responsive Cell Culture Surfaces
Yang et al.,
2002
Fabrication and surface modification of
macroporous poly(L-lactic acid) and poly(L-lactic-
co-glycolic acid) (70/30) cell scaffolds for human
skin fibroblast cell culture
Shao et al.,
2002
They describe a method, termed SILAC, for stable
isotope labeling by amino acids in cell culture, for
the in vivo incorporation of specific amino acids
into all mammalian proteins
Brinster.,
2002
The recently developed testis cell
transplantation method provides a powerful
approach to studying the biology of the male
germline stem cell and its microenvironment,
the stem cell niche.
Bhadriraju
& Chen.,
2002
They described a review about engineering
cellular microenvironments to improve cell-
based drug testing
Shinohar
a et al.,
2003
Long-Term Proliferation in Culture and Germline Transmission of
Mouse Male Germline Stem Cells- Long-term spermatogonial stem
cell culture will be useful for studying spermatogenesis mechanism
and has important implications for developing new technology in
transgenesis or medicine.
Park &
Shuler.,
2003
Integration of Cell Culture and Microfabrication Technology-
Recent progress in cell culture and microfabrication technologies
has contributed to the development of cell-based biosensors for the
functional characterization and detection of drugs, pathogens,
toxicants, and odorants.
Uemuraa
et al.,
2003
Transplantation of cultured bone cells using combinations of
scaffolds and culture techniques-will a fine step toward bone
regeration therapies.
Beltrami et
al., 2003
They established that Adult Cardiac Stem Cells Are
Multipotent and Support Myocardial Regeneration
Thomas et
al., 2003
Homologous recombination in human embryonic stem cells-
these facts has revolutionized the study of gene function in
mammals, Although most often used to generate knockout
mice, homologous recombination has also been applied in
mouse ES cells allowed to differentiate in vitro. Homologous
recombination is an essential technique if human ES cells are
to fulfill their promise as a basic research tool.
De Ugarte
et al., 2003
They presented the comparison of Multi-Lineage Cells from
Human Adipose Tissue and Bone Marrow- May find their
way in tissue-engineering applications and as gene delivery
vehicles.
Pittenger.,
et al ., 2004
Mesenchymal Stem Cells and Their Potential as
Cardiac Therapeutics, hMSCs isolated from adult bone
marrow provide an excellent model for development of
stem cell therapeutics, and their potential use in the
cardiovascular system is currently under investigation
in the laboratory and clinical settings.
Kohji et al.,
2004
Functional bioengineered corneal epithelial sheet grafts
from corneal stem cells expanded ex vivo on a
temperature-responsive cell culture surface- they have
developed a novel cell-sheet manipulation technology
using temperature-responsive culture surfaces to
generate functional, cultivated corneal epithelial cell
sheet grafts.
Sin et al.,
2004
The Design and Fabrication of Three-Chamber
Microscale Cell Culture Analog Devices with
Integrated Dissolved Oxygen Sensors- provides an in
vitro supplement to animal studies and the possibility
of a human surrogate for predicting human response
in clinical trials.
Kunz-
Schughart
et al.,
2004
The Use of 3-D Cultures for High-Throughput
Screening: The Multicellular Spheroid Model- detect
specific cellular effects reflecting action at various
targets.
Cowan et
al., 2004
Adipose-derived adult stromal cells heal critical-size
mouse calvarial defects- heal critical-size skeletal
defects without genetic manipulation or the addition
of exogenous growth factors.
Fok &
Zandstra.,
2005
Shear-Controlled Single-Step Mouse Embryonic Stem
Cell Expansion and Embryoid Body–Based
Differentiation
These cell-culture tools should prove useful for both the
production of ESCs and ESC-derived cells and for
investigations into adhesion, survival, and differentiation
phenomena during ESC propagation and differentiation.
Jonathan
et al., 2005
Computer-Controlled Microcirculatory Support System
for Endothelial Cell Culture and Shearing was
developed
Fulcher et
al., 2005
Well-Differentiated Human Airway Epithelial Cell
Cultures
Barbulovic
-Nad et al.,
2010
A microfluidic platform for complete mammalian cell
culture- The automated cell microculture technique
Wang et
al., 2005
The Bioreactor: A Powerful Tool for Large-Scale Culture of
Animal Cells
Yaakov et
al., 2005
Laser-guided direct writing for three-dimensional tissue
engineering- underlying processes such as liver and
pancreas morphogenesis, differentiation, and angiogenesis.
Xu et al.,
2005
Inkjet printing of viable mammalian cells
The computer-aided inkjet printing of viable mammalian
cells holds potential for creating living tissue analogs, and
may eventually lead to the construction of engineered
human organs.
Franken et
al., 2006
Clonogenic assay of cells in vitro-colony formation
assay is an in vitro cell survival assay based on the
ability of a single cell to grow into a colony.
Shah et al.,
2006
NucleoCounter—An efficient technique for the
determination of cell number and viability in animal
cell culture processes
Alaminos et
al., 2006
Construction of a Complete Rabbit Cornea Substitute
Using a Fibrin-Agarose Scaffold
Klimanskay
a et al.,
2006,
Human embryonic stem cell lines derived from single
blastomeres
Pampalon
i et al.,
2007
They described that the third dimension bridges
the gap between cell culture and live tissue- they
believe that 3D cultures will have a strong impact
on drug screening and will also decrease the use of
laboratory animals, for example, in the context of
toxicity assays.
Parker &
Townley.,
2007
Biomimetics of photonic nanostructures in cell
culture.
the nanoengineering efficiency of living cells is
harnessed and natural organisms such as diatoms
and viruses are used to make nanostructures that
could have commercial applications.
Chatterje
ea et al.,
2008
Upconversion fluorescence imaging of cells and small
animals using lanthanide doped nanocrystals
this represents the first demonstration of use of
upconversion fluorophores for cellular and tissue
imaging.
Shinohara
et al., 2008
Long-Term Culture of Male Germline Stem Cells
From Hamster Testes.
This technique can be extended to other animal species
in which current genetic modification techniques are
impossible or inefficient.
Paguirigan
& Beebe.,
2008
Microfluidics meet cell biology: bridging the
gap by validation and application of microscale
techniques for cell biological assays
Li et al., 2008 Germline Competent Embryonic Stem Cells
Derived from Rat Blastocysts
Establishment of authentic rat ES cells will
make possible sophisticated genetic
manipulation to create models for the study of
human diseases.
Davis et al .,
2009
Validation of the Cardiosphere Method to Culture Cardiac
Progenitor Cells from Myocardial Tissue
Castell &
Gómez-
Lechón.,
2009
Liver cell culture techniques.
Mesimäki et
al., 2009
Novel maxillary reconstruction with ectopic bone formation
by GMP (good manufacturing practice) adipose stem cells
This is the first clinical case where ectopic bone was
produced using auto ASCs (adipose stem cells) in
microvascular reconstruction surgery and it will pave way for
new clinical trials in the field.
Hiramia et
al., 2009
Generation of retinal cells from mouse and human induced
pluripotent stem cells
Which have a possibility to be used as patient-specific donor cells for transplantation therapies?
Zhao et al.,
2009
iPS cells produce viable mice through tetraploid
complementation.
They reported the generation of several iPS cell lines
that are capable of generating viable, fertile live-born
progeny by tetraploid complementation.
Friedrich et
al., 2009
Spheroid-based drug screen: considerations and
practical approach.
Monitoring of spheroid growth kinetics to determine
growth delay and re-growth, after drug treatment
requires long-term culturing
Fernandes
et al., 2009
Successful scale-up of human embryonic stem cell
production in a stirred microcarrier culture system
Haycock .,
2010
3D Cell Culture: A Review of Current Approaches
and Techniques
Younga &
Beebe., 2010
Fundamentals of microfluidic cell culture in
controlled microenvironments
Uygun et al.,
2010
Organ reengineering through development of a
transplantable recellularized liver graft using
decellularized liver matrix
Sasagawa et
al., 2010
Design of prevascularized three-dimensional cell-
dense tissues using a cell sheet stacking
manipulation technology
Huh et al.,
2011
From 3D cell culture to organs-on-chips-
These ‘organs-on-chips’ permit the study of
human physiology in an organ-specific context,
enable development of novel in vitro disease
models, and could potentially serve as
replacements for animals used in drug
development and toxin testing.
Tekkatte et
al., 2011
“Humanized” Stem Cell Culture Techniques:
The Animal Serum Controversy
Louch et al.,
2011
Methods in cardiomyocyte isolation, culture, and
gene transfer-
Gene transfer techniques for neonatal and adult
cardiomyocytes are also studied, including methods
for transfection (liposome, electroporation) and
viral-based gene delivery.
Centeno et
al., 2011
Safety and Complications Reporting Update on the
Re-Implantation of Culture-Expanded
Mesenchymal Stem Cells Using Autologous
Platelet Lysate Technique
Jesus &
Wurm., 2011
Manufacturing recombinant proteins in kg-ton
quantities using animal cells in bioreactors
Atala., 2011 Tissue engineering of human bladder- A review
AlGhamdi et
al., 2012
Low-level laser therapy: a useful technique for
enhancing the proliferation of various cultured cells
Greiner et al.,
2012
Micro-Engineered 3D Scaffolds for Cell Culture
Studies
Here fabrication techniques, materials, architectures,
biochemical functionalizations, and mechanical
properties of 3D scaffolds were discussed.
Booth et al.,
2012
Acellular Normal and Fibrotic Human Lung Matrices
as a Culture System for In Vitro Investigation
Czekanska et
al., 2012
In Search Of An Osteoblast Cell Model For In Vitro
Research-A review
Castro et al.,
2013
High-throughput SNP-based authentication of
human cell lines (a Solution for cell line mis-
identifications)
Kawamura et
al., 2013
Enhanced Survival of Transplanted Human Induced
Pluripotent Stem Cell–Derived Cardiomyocytes by
the Combination of Cell Sheets with the Pedicled
Omental Flap Technique in a Porcine Heart
Page et al.,
2013
Three-dimensional tissue cultures: current trends
and beyond –A Review
This short review discusses various cell culture
approaches to improve the simulation of the 3D
environment of cells.
Dayeh et al.,
2013
The Use of Fish-Derived Cell Lines for
Investigation of Environmental Contaminants.
Kalos &
June ., 2013
Adoptive T-Cell Transfer for Cancer
Immunotherapy in the Era of Synthetic Biology
Kanatsu-
Shinohara
&Takashi .,
2013
Spermatogonial Stem Cell Self-Renewal and
Development
It will enable us to use these cells for applications in
animal transgenesis and medicine.
Li et al., 2013 Photostable fluorescent organic dots with
aggregation-induced emission (AIE dots) for non-
invasive long-term cell tracing
Landauer., 2014 Designing media for animal cell culture: CHO
cells, the industrial standard.
Kremkow &
Lee., 2015
Sequencing technologies for animal cell
culture research.
Takahashi et
al., 2015
Gene Transfer into Cultured Mammalian
Embryos by Electroporation
Human Genome Projects
Rich media development
Genetic manipulation of cell lines
Development of increasingly sophisticated incubators
Vaccine design
Development of new adhesion surfaces
3D scaffolds
Biotherapeutics
Tissue engineering (for example, the production of tissue in vitro as
skin or cartilage for treatment of burns, auto-grafting, differentiation and induced differentiation (Naderi et al., 2011).
Three-dimensional (3D) cell culture models
(Smalley et al., 2008)
Biorobotics (Zorlutuna et al., 2013).
Cell line misidentifications
Protein expression technologies
New clone screening technologies.
Artificial Chromosome Expression (ACE) System
There is growing interest in controlling matrix stiffness, a concept that has led to discoveries in fields such as:
Stem cell self-renewal
Lineage specification
Cancer cell phenotype
Fibrosis
Hepatocyte function
Mechano-sensing
The in vitro micronucleus technique (Michael.,2001)
Dynamic macroscale bioreactor systems