Adsorbents · 2020-02-21 · 5 Sorbtech. Premium Rf™ is the finest granular silica gel on the...

Sorbent Technologies, Inc. | www.sorbtech.com | 770-936-0323 | 5955 Peachtree Corners E, Norcross, GA 300712

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Adsorbents


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Sorbent Technologies offers a full range of spherical and granular silica gels in premium and standard grades. This diverse selection allows our customers to perform simple to complex analytical and preparative chromatographic techniques for laboratory, pilot and industrial process applications.

Finding appropriate adsorbents is easy and convenient. Allow a Sorbtech Product Specialist to help you select a silica gel to best fit your separation needs.

• Spherical and granular shapes• Particle sizes for analytical to preparitive to process applications from 2.0µm to 3mm• Pore sizes for small to large molecule separations (proteins and peptides)from 60Å to 1000Å• C18, C8, C4, C2, Amino, Cyano, Phenyl, Diol, WAX, SAX, WCX, SCX, Thiol, and Custom Bonded Phases

SILICA GEL SELECTION GUIDEThe solubility of the targeted compound to be purified contributes to the determination of the functionality of the silica gel.

FUNCTIONALITY:

CAPACITY:

SELECTIVITY:

The molecular weight of the targeted compound establishes the proper pore size of the silica gel to optimize the capacity.

The resolution factor defines the number of theorretical plates required to perform the separation, and gives an indication about the particle size to be selected.

COMPOUNDTO BE

PURIFIED

FUNCTIONALITY

CHEMISTRYSOLUBILITY

nON aQUEOUS

SILICA C4 & C8 C18

NON TO LOWPOLARITY

LOW TOMEDIUM

POLARITYMEDIUM TO

HIGH POLARITY

aQUEOUS

CAPACITY

MOLECULARWEIGHT

MW<1000D MW<10,000D MW<50,000D

POLYPEPTIDESBIO-POLYMERSPEPTIDES

A < 1.2 1.2 < A < 1.5 1.5 < A < 2.0 A < 2.0

3 & 5ųM10ųM15ųM

20ųM40ųM

20-45ųM40-75ųM 75-200ųMORGANIC

MOLECULES

500Å800Å

1000Å

100Å200Å300Å

60Å70Å

SEPARATIONDIFFICULTY

SELECTIVITY

Silica Gel

5Sorbtech

Separate. Purify. Discover.

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Premium Rf™ is the finest granular silica gel on the market. This high purity product provides narrow particle and pore distribution and is designed to increase yield, making it ideal for every-day chromatography and complicated separations.

Recommended Applications

• Aflotoxins• Chloramphenicol• Pesticides• Steroids• Vitamins

Granular or irregular-shaped silica gel is commonly used for the following applications: Medium Pressure Liquid Chromatography (MPLC), flash chromatography, and classic column chromatography. Silica gels used for these applications are normally 60Å or sometimes 100Å. For larger porosities please visit our Spherical Silica Gel pages in this catalog or go to www.sorbtech.com. For bonded phase silica gel, see the Functionalized Silica Gel Section.

Advantages of Premium RF Flash Silica Gel

Granular Silica Gel TLC, MPLC, Flash, & Gravity LC Phases

Narrow Particle Distribution: 40-75µm +/-5% versus leading brands +/-10-12%.• Easier packing• No channeling in the bed, creating tighter bands, and better separation• Lower pressure drop (fewer fine particles), for faster flow and faster run time1

Narrow pore size distribution: minimum small pores• Maximum targeted surface area availablem for greater loadability• Minimum risk of product trapped in pores, for increased yield and longer life time of the silica gel3

Side by side comparison of tests of every major silica gel show our Premium Rf Delivers the best results• Easier to pack• More fractions• Better resolution

• Increased yield• Faster run time,

better kinetics5

Purity 99.95% SiO2• Our high purity (low metal impurities) ensures maximum

resolution as compared to competitive offerings4

Small pore volume: higher bulk density.• More silica gel per column volume, for greater loadability• Lower dead volume, leading to less solvent use2

• Bulk quantities for large-scale applications• No scale-up limitation• Secured lots offered for process use

With Premium Rf your lab can enjoy:


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GranularParticle

Dist. Porosity pH Value LOI Bulk

Density Surface AreaWater

Soluble Matter

52200 Premium Rf 15 - 40 µm 60Å ~ 7 5% 0.5 g/ml 450-550 m2/g -52300 Premium Rf 20 - 45 µm 60Å ~ 7 5% 0.5 g/ml 450-550 m2/g -52500 Premium Rf 40 - 75 µm 60Å ~ 7 5% 0.5 g/ml 470-570 m2/g -52700 Premium Rf 75 - 200 µm 60Å ~ 7 5% 0.5 g/ml 470-570 m2/g -

40930M Premium Grade 40 - 63 µm 60Å ~ 7 2 - 4% 0.5 g/ml 460-550 m2/g -11610 Standard Grade 5 - 15 µm 60Å ~ 7 - 0.4 g/ml 500-600 m2/g 0.2%10910 Standard Grade 12 - 26 µm 60Å ~ 7 - 0.4 g/ml 500-600 m2/g 0.2%10920 Standard Grade 18 - 32 µm 60Å ~ 7 - 0.4 g/ml 500-600 m2/g 0.2%10930 Standard Grade 32 - 63 µm 60Å ~ 7 - 0.4 g/ml 500-600 m2/g 0.2%

30930M Standard Grade 40 - 63 µm 60Å ~ 7 < 9% 0.5 g/ml 450-550 m2/g -40930 Standard Grade 40 - 63 µm 60Å ~ 7 < 7% 0.5 g/ml 450-550 m2/g -10940 Standard Grade 63 - 200 µm 60Å ~ 7 - 0.5 g/ml 500-600 m2/g 0.2%30940 Standard Grade 63 - 200 µm 60Å ~ 7 < 9% 0.5 g/ml 450-550 m2/g -40940 Standard Grade 63 - 200 µm 60Å ~ 7 < 7% 0.5 g/ml 450-550 m2/g -10400 Standard Grade 100 - 200 µm 60Å ~ 7 - 0.5 g/ml 500-600 m2/g 0.2%10950 Standard Grade 200 - 500 µm 60Å ~ 7 - 0.5 g/ml 500-600 m2/g 0.2%10800 Standard Grade, Active 18 - 32 µm 60Å ~ 7 < 5% 0.4 g/ml 500-600 m2/g 0.2%10810 Standard Grade, Active 32 - 63 µm 60Å ~ 7 < 5% 0.4 g/ml 500-600 m2/g 0.2%10820 Standard Grade, Active 32 - 100 µm 60Å ~ 7 < 5% 0.5 g/ml 500-600 m2/g 0.2%10830 Standard Grade, Active 63 - 100 µm 60Å ~ 7 < 5% 0.5 g/ml 500-600 m2/g 0.2%10840 Standard Grade, Active 63 - 200 µm 60Å ~ 7 < 5% 0.5 g/ml 500-600 m2/g 0.2%10850 Standard Grade, Active 100 - 200 µm 60Å ~ 7 < 5% 0.5 g/ml 500-600 m2/g 0.2%10860 Standard Grade, Active 200 - 500 µm 60Å ~ 7 < 5% 0.5 g/ml 500-600 m2/g 0.2%

TIPS & TRICKS

Granular Silica Gel TLC, MPLC, Flash, & Gravity LC Phases

To standardize silica gel from chemist to chemist, lot to lot, run to run, place the amount of silica gel you are using into a vacuum oven and heat for 30-45 min. at 130° C. Cool to room temperature, then pack your column. This will eliminate any residual moisture.

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CAT. # DESCRIPTION PARTICLE DIST. SIZE

11610-05 Silica Gel for TLC, Standard Grade, 60 Å 5 - 15 µm 500 g11610-25 Silica Gel for TLC, Standard Grade, 60 Å 5 - 15 µm 25 kg11620-05 Silica Gel for TLC, Standard Grade, 60 Å, w/UV254 5 - 15 µm 500 g11620-25 Silica Gel for TLC, Standard Grade, 60 Å, w/UV254 5 - 15 µm 25 kg11630-05 Silica Gel for TLC, Standard Grade, 60 Å, w/ Gypsum 5 - 15 µm 500 g11630-25 Silica Gel for TLC, Standard Grade, 60 Å, w/ Gypsum 5 - 15 µm 25 kg11640-05 Silica Gel for TLC, Standard Grade, 60 Å, w/ Gypsum and UV254 5 - 15 µm 500 g11640-25 Silica Gel for TLC, Standard Grade, 60 Å, w/ Gypsum and UV254 5 - 15 µm 25 kg52200-1 Silica Gel for Prep. TLC, Premium Rf, 60 Å 15 - 40 µm 1 kg52200-25 Silica Gel for Prep. TLC, Premium Rf, 60 Å 15 - 40 µm 25 kg11650-1 Silica Gel for Prep. TLC, 60 Å, w/UV254 5 - 50 µm 1 kg11650-5 Silica Gel for Prep. TLC, 60 Å, w/UV254 5 - 50 µm 5 kg11660-1 Silica Gel for Prep. TLC, 60 Å, w/Gypsum and UV254 5 - 50 µm 1 kg11660-5 Silica Gel for Prep. TLC, 60 Å, w/Gypsum and UV254 5 - 50 µm 5 kg


10910-05 Silica Gel for MPLC, Standard Grade, 60 Å 12 - 26 µm 500 g10910-1 Silica Gel for MPLC, Standard Grade, 60 Å 12 - 26 µm 25 kg

10910-2.5 Silica Gel for MPLC, Standard Grade, 60 Å 12 - 26 µm 500 g10910-5 Silica Gel for MPLC, Standard Grade, 60 Å 12 - 26 µm 25 kg10910-25 Silica Gel for MPLC, Standard Grade, 60 Å 12 - 26 µm 500 g10920-05 Silica Gel for MPLC, Standard Grade, 60 Å 18 - 32 µm 25 kg10920-1 Silica Gel for MPLC, Standard Grade, 60 Å 18 - 32 µm 500 g

10920-2.5 Silica Gel for MPLC, Standard Grade, 60 Å 18 - 32 µm 25 kg10920-5 Silica Gel for MPLC, Standard Grade, 60 Å 18 - 32 µm 1 kg10920-25 Silica Gel for MPLC, Standard Grade, 60 Å 18 - 32 µm 25 kg52300-05 Silica Gel for MPLC, Premium Rf, 60 Å 20 - 45 µm 1 kg52300-1 Silica Gel for MPLC, Premium Rf, 60 Å 20 - 45 µm 5 kg

52300-2.5 Silica Gel for MPLC, Premium Rf, 60 Å 20 - 45 µm 2.5 kg52300-5 Silica Gel for MPLC, Premium Rf, 60 Å 20 - 45 µm 5 kg

52300-25 Silica Gel for MPLC, Premium Rf, 60 Å 20 - 45 µm 25 kg

Sorbtech offers an assortment of silica gels for making analytical and preparative TLC plates. Our silica gel for TLC (Cat. #11610) and silica gel for prep TLC (Cat. #52200) contain neither an inorganic nor an organic binder. They can also be used in a packed column format for extra high resolution.

For more selections and sizes, visit our website or contact your personal account manager.

Improve Your Resolution with MPLCWhen working with difficult separations that provide very low Rf-values on initial screenings by TLC and require larger volumes of flash grade silica gel, consider the improved resolution power that Sorbtech MPLC grades deliver. By using a smaller particle size, a higher number of theoretical plates are achieved using the same volume of silica gel, which increases the resolution.

The thickness of the absorbent layer is typically around 0.1 – 0.25 mm for analytical purposes and around 0.5 – 2.0 mm for preparative TLC.

TLC slurry preparation for soft layer coating requires:1. Silica Gel and small amount of calcium sulfate (gypsum) is mixed with water.2. This mixture is spread as a thick slurry on a clean glass plate.3. The resultan plate is dried and activated by heating in an oven for thirty minutes at 110°C.

TLC Silica Gels

MPLC Silica Gels

Granular

Granular


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Sorbtech offers high-quality granular silica gels for flash chromatography with an extensive range of silicas gels that have been selected for high performance and optimal reproducibility.

Sorbtech offers high-quality granular silica gels for flash chromatography with an extensive range of silicas gels that have been selected for high performance and optimal reproducibility.

* For reverse phase silica gels see the Functionalized Silica Gels Section. For more selections and sizes, visit our website or contact your personal account manager.

CAT. # DESCRIPTION GRADE PARTICLE DIST. SIZE

52500-05 Silica Gel, Premium Rf, 60 Å Flash 40-75 μm (200x400 mesh) 500 g52500-1 Silica Gel, Premium Rf, 60 Å Flash 40-75 μm (200x400 mesh) 1 kg

52500-2.5 Silica Gel, Premium Rf, 60 Å Flash 40-75 μm (200x400 mesh) 2.5 kg52500-5 Silica Gel, Premium Rf, 60 Å Flash 40-75 μm (200x400 mesh) 5 kg

52500-25 Silica Gel, Premium Rf, 60 Å Flash 40-75 μm (200x400 mesh) 25 kg40930M-05 Silica Gel, Premium, 60 Å Flash 40-63 μm (230x400 mesh) 500 g40930M-1 Silica Gel, Premium, 60 Å Flash 40-63 μm (230x400 mesh) 1 kg

40930M-2.5 Silica Gel, Premium, 60 Å Flash 40-63 μm (230x400 mesh) 2.5 kg40930M-5 Silica Gel, Premium, 60 Å Flash 40-63 μm (230x400 mesh) 5 kg

40930M-25 Silica Gel, Premium, 60 Å Flash 40-63 μm (230x400 mesh) 25 kg30930M-05 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 500 g30930M-1 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 1 kg

30930M-2.5 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 2.5 kg30930M-5 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 5 kg

30930M-25 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 25 kg40930-05 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 500 g40930-1 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 1 kg

40930-2.5 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 2.5 kg40930-5 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 5 kg

40930-25 Silica Gel, Standard Grade, 60 Å Flash 40-63 μm (230x400 mesh) 25 kg10930-25 Silica Gel, Standard Grade, 60 Å Flash 32-63 μm (230x450 mesh) 25 kg

CAT. # DESCRIPTION GRADE PARTICLE DIST. SIZE

52700-05 Silica Gel, Premium Rf, 60 Å Gravity 75-200 μm (70x200 mesh) 500 g52700-1 Silica Gel, Premium Rf, 60 Å Gravity 75-200 μm (70x200 mesh) 1 kg

52700-2.5 Silica Gel, Premium Rf, 60 Å Gravity 75-200 μm (70x200 mesh) 2.5 kg52700-5 Silica Gel, Premium Rf, 60 Å Gravity 75-200 μm (70x200 mesh) 5 kg

52700-20 Silica Gel, Premium Rf, 60 Å Gravity 75-200 μm (70x200 mesh) 20 kg52700-25 Silica Gel, Premium Rf, 60 Å Gravity 75-200 μm (70x200 mesh) 25 kg30940-05 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 500 g30940-1 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 1 kg

30940-2.5 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 2.5 kg30940-5 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 5 kg

30940-25 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 25 kg40940-05 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 500 g40940-1 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 1 kg

40940-2.5 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 2.5 kg40940-5 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 5 kg

40940-25 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 25 kg10940-25 Silica Gel, Standard Grade, 60 Å Gravity 63-200 μm (70 x 230 mesh) 25 kg

Flash Silica Gels

Gravity Silica Gels

Granular

Granular

9Sorbtech


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Silica Gel for ASTM D1319-02a and ISO 3037

Cat #51600, Silica Gel, Premium Rf, 30 Å, 75-150 μm - Typical Product Specifications

Quality Silica GelOur silica gel #51600 is a high purity silica and is manufactured to conform to the specifications of ASTM D1319-02a test method for hydrocarbon types in liquid petroleum products by fluorescent indicator adsorption and ISO 3037 liquid petroleum products–determination of hydrocarbon types-fluorescent indicator adsorption method. Sorbtech silica gel #51600 can be used in place of W.R. Grace’s product (Code 923.) See table below.

ASTM D1319-02a (Test method)This method covers the determination of hydrocarbon types over the concentration ranges from 5 to 99 volume % aromatics, 0.3 to 55 volume % olefins, and 1 to 95 volume % saturates in petroleum fractions that distill below 315° C. This test method may apply to concentrations outside these ranges, but the precision has not been determined. Samples containing dark-colored components that interfere in reading the chromatographic bands cannot be analyzed.

ISO 3037 (Test method)The determination of the total volume percent [% (V/V)] of saturates, olefins, and aromatics in petroleum fractions is important in characterizing the quality of petroleum fractions as gasoline blending components. As feeds to catalytic reforming processes, and in characterizing petroleum fractions and products from catalytic reforming and from thermal and catalytic cracking as blending components for motor and aviation fuels. This information is also important in measuring of the quality of aviation turbine fuels.

ITEM UNIT ASTM D1319-02a RESULTS

Year 2002 -Surface Area m²/g 450 - 530 515

pH 5.5 - 7.0 5.99Loss of Ignition mass % 4.5 - 10.0 4.87

Fe2O3 Mass ppm 50 max -Particle Distribution

on 250 µm 0 0on 180 µm 1.2 max 0on 150 µm 5 max 0

75 to 150 µm 1.2 max 94.4Thru 75 µm 5.6

Pore Volume ml/g 15 max 0.39H₂O % - 0.66

Laser APS µm - 141.23SiO₂ % - 99.95Na ppm - 29Mg ppm - 0.8AI ppm - 5Ca ppm - 5.1Fe ppm - 3.8Zr ppm - 15Ti ppm - 102Cr ppm - 0.18Mn ppm - 0.02Co ppm - 0.17Ni ppm - 0.06Zn ppm - 0.2Cd ppm - 0.02Pb ppm - 0.13


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Why choose spherical over granular?This study of a large particle size flash-grade demonstrates the advatages of a spherical silica gel over irregular silica gel. Due to the significant difference in the manufacturing processes between spherical and irregular silica, the comparison of physical properties, particle size distribution and chemical properties clearly highlights the benefits of the spherical silica in terms of loading, efficiency, bed stability, pressure drop, tailing and life time. A flash chromatography test, and an adsorption chromatography test, demonstrates the rewards of utilizing spherical silica gel for flash chromatography even with larger particle size. The advatages of the spherical silica gel can allow the chromatographer to significantly improve their flash chromatography step using existing equipment without having to switch the purification process to costly HPLC equipment.

ITEMS UNIT SPHERICAL GRANULAR

Surface Area (m2/g) 272 281Pore Volume (ml/g) 0.81 1.14Bulk Density (g/ml) 0.51 0.40

Loss on Drying (%) 6.43 1.65pH (5% suspension) - 7.8 7.5Specific Resistance (Ωcm) 57,100 15,700

Transparency (%) 76 33


Na µg/g 298 530Ca µg/g 90 1100Fe µg/g 18 60

ITEMS SPHERICAL GRANULAR

Column Height (mm) 172 235Retention Time

(DMP) min 10.61 11.44

k' (DMP) 2.48 3.50N (DMP) 241 118

Symmetry (DMP) 1.6 2.1

PARTICLE SIZE


d50 (Average) µm 103 143d10 µm 141 228d90 µm 80 9

d10/d90 - 1.8 2.5

Spherical Silica Gels HPLC, MPLC, Flash, & Gravity LC Phases

Physical Properties• Spherical has lower pore-volume and high

bulk density (Higher loading in the same column volume)

• Spherical has high specific resistance (Low impurity content)

• Spherical has high transparency (Low content of fine particles)

Chemical Properties/Impurities• Spherical has lower impurity levels,

especially for calcium (No undesirable tailing of polar compounds)

1

3

Spherical Silica shows higher resolution. The reasons are:

• Smaller Particle Size• Homogenous packing due to

the spherical particle shape.

20 x 500 mm glass column25 g

n-hexane / ethyl acetate (90-10) w%5 cm/min (15.7 ml /min)UV 254 nm

Column:Sample Weight:

Mobile Phase:Flow Rate:Detection:

1. Benzene 2. Dimethyl phthalate

Flash Chromatography Test• Flash Chromatography Conditions:4

Particle Size Distribution• Spherical has lower mean particle size and

sharper distribution (Higher efficiency and lower pressure drop and easier flow rate)

• Spherical Shape (Stable Chromatography bed & no fine generations)

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ADSORPTION TIMES UNIT SPHERICAL GRANULAR

Point A (min.) 24.0 19.3Point B (min.) 25.3 21.5

Segment A-B (min.) 1.3 2.2Column Capacity (mg) 312 251Silica Capacity (mg/g) 146 155

Capacity of dimethyl phthalate (DMP):2 ml/min0.65 g/ml or 1.3 g/min13 mg/min13 x A

Flow Rate:d (solvent):DMP (1%):

Capacity (mg):

• C-E: Elution Time• D-E: Concentration change time (Mass Transfer zone)

ELUTION TIMES UNIT SPHERICAL GRANULAR

Point C (min.) 2.3 1.9Point D (min.) 5.8 4.1Point E (min.) 10.5 10.8

Segment C-E (min.) 8.2 8.9Segment D-E (min.) 4.7 6.7

Adsorption Curve• A long break through time (point A) indicates higher capacity.• A short mass transfer time (A-B) indicates higher equilibrium.• Both silica gels were packed in 4.6 x 250 mm stainless steel columns by dry packing.5a

Elution/Desorption Curve

• A long break through time (point A) indicates higher capacity.• A short mass transfer time (D-E) indicates lower solvent consumption.

5b Initially the columns were washed out by n-hexane at the flow rate of 2ml/min. The columns were then switched to an adsorption solvent and monitored by UV detection. We first measured the adsorption up to the point A (capacity), and then we measured the mass tranfer up to the point B (equilibrium):

ConclusionWhen using spherical silica gel you will have:• Increased loading - up to 30% more• Greater reproducibility of process• Sharper peaks and cost savings (less solvent

consumption and labor

• Increased yield with tighter pore symmetry• Longer bed lifetime (decrease cost of labor and

silica consumption)• Better chromatographic results without switching to

costly HPLC equipment

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High purity silica gel for normal phase column chromatography. Also used as base material for bonded phases.

CAT. # PARTICLE DIST. POROSITY pH VALUE LOSS ON

DRYINGBULK

DENSITY

SUR-FACE AREA

PORE VOLUME

COMPOUND SIZE

72200 5 µm 70 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 500 m²/g 0.80 ml/g <1,000 Daltons73200 5 µm 100 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 270 m²/g 0.80 ml/g <10,000 Daltons74200 5 µm 150 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 175 m²/g 0.80 ml/g <10,000 Daltons75200 5 µm 200 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 130 m²/g 0.80 ml/g <10,000 Daltons76200 5 µm 300 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 90 m²/g 0.80 ml/g <10,000 Daltons77200 5 µm 500 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 60 m²/g 0.80 ml/g <50,000 Daltons78200 5 µm 800 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 45 m²/g 0.80 ml/g <50,000 Daltons79200 5 µm 1000 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 30 m²/g 0.80 ml/g <50,000 Daltons72300 10 µm 70 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 500 m²/g 0.80 ml/g <1,000 Daltons73300 10 µm 100 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 270 m²/g 0.80 ml/g <10,000 Daltons74300 10 µm 150 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 175 m²/g 0.80 ml/g <10,000 Daltons75300 10 µm 200 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 130 m²/g 0.80 ml/g <10,000 Daltons76300 10 µm 300 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 90 m²/g 0.80 ml/g <10,000 Daltons77300 10 µm 500 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 60 m²/g 0.80 ml/g <50,000 Daltons78300 10 µm 800 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 45 m²/g 0.80 ml/g <50,000 Daltons79300 10 µm 1000 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 30 m²/g 0.80 ml/g <50,000 Daltons72400 15 µm 70 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 500 m²/g 0.80 ml/g <1,000 Daltons73400 15 µm 100 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 270 m²/g 0.80 ml/g <10,000 Daltons74400 15 µm 150 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 175 m²/g 0.80 ml/g <10,000 Daltons75400 15 µm 200 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 130 m²/g 0.80 ml/g <10,000 Daltons76400 15 µm 300 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 90 m²/g 0.80 ml/g <10,000 Daltons77400 15 µm 500 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 60 m²/g 0.80 ml/g <50,000 Daltons78400 15 µm 800 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 45 m²/g 0.80 ml/g <50,000 Daltons79400 15 µm 1000 Å 4.0 - 7.0 ≤ 2.0% 0.45 g/ml 30 m²/g 0.80 ml/g <50,000 Daltons72600 20 - 45 µm 70 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 500 m²/g 0.80 ml/g <1,000 Daltons73600 20 - 45 µm 100 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 270 m²/g 0.80 ml/g <10,000 Daltons74600 20 - 45 µm 150 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 175 m²/g 0.80 ml/g <10,000 Daltons75600 20 - 45 µm 200 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 130 m²/g 0.80 ml/g <10,000 Daltons76000 20 - 45 µm 300 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 90 m²/g 0.80 ml/g <10,000 Daltons77600 20 - 45 µm 500 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 60 m²/g 0.80 ml/g <50,000 Daltons78600 20 - 45 µm 800 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 45 m²/g 0.80 ml/g <50,000 Daltons79600 20 - 45 µm 1000 Å 6.0 - 8.0 ≤ 2.0% 0.45 g/ml 30 m²/g 0.80 ml/g <50,000 Daltons62500 40 - 75 µm 70 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 500 m²/g 0.80 ml/g <1,000 Daltons63500 40 - 75 µm 100 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 270 m²/g 0.80 ml/g <10,000 Daltons66500 40 - 75 µm 300 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 90 m²/g 0.80 ml/g <10,000 Daltons67500 40 - 75 µm 500 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 60 m²/g 0.80 ml/g <50,000 Daltons68500 40 - 75 µm 800 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 45 m²/g 0.80 ml/g <50,000 Daltons69500 40 - 75 µm 1000 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 30 m²/g 0.80 ml/g <50,000 Daltons62700 75 - 200 µm 70 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 500 m²/g 0.80 ml/g <1,000 Daltons63700 75 - 200 µm 100 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 270 m²/g 0.80 ml/g <10,000 Daltons66700 75 - 200 µm 300 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 90 m²/g 0.80 ml/g <10,000 Daltons67700 75 - 200 µm 500 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 60 m²/g 0.80 ml/g <50,000 Daltons68700 75 - 200 µm 800 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 45 m²/g 0.80 ml/g <50,000 Daltons69700 75 - 200 µm 1000 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 30 m²/g 0.80 ml/g <50,000 Daltons63900 200 - 500 µm 100 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 270 m²/g 0.80 ml/g <10,000 Daltons64900 200 - 500 µm 150 Å 6.0 - 8.0 3.0 -7.0% 0.5 g/ml 175 m²/g 0.80 ml/g <10,000 Daltons

Spherical Silica Gels HPLC, MPLC, Flash, & Gravity LC Phases

13Sorbtech


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Sorbtech offers HPLC silica gels in two grades (Premium Pure™ and Premium Rf™.) These grades are ideally suited for normal phase chromatography for both analytical and preparative HPLC. Additionally, the high purity, characterized by low metal ion content and low salt concentrations, offer a strong backbone for functionalization (e.g. C18, C8, Amino, etc.). The porosities range from 70 Å to 1000 Å in particle sizes of 2μm, 3μm, 5μm, 10μm, and 15μm.

CAT. # DESCRIPTION POROSITY PARTICLE DIST. SIZE

10301500-001 Spherical Silica Gel, Premium Pure 100 Å 2 µm 10 g10302000-001 Spherical Silica Gel, Premium Pure 100 Å 3 µm 10 g10303000-001 Spherical Silica Gel, Premium Pure 100 Å 5 µm 10 g10304000-001 Spherical Silica Gel, Premium Pure 100 Å 10 µm 10 g10352000-001 Spherical Silica Gel, Premium Pure 150 Å 3 µm 10 g10353000-001 Spherical Silica Gel, Premium Pure 150 Å 5 µm 10 g10354000-001 Spherical Silica Gel, Premium Pure 150 Å 10 µm 10 g10402000-001 Spherical Silica Gel, Premium Pure 200 Å 3 µm 10 g10403000-001 Spherical Silica Gel, Premium Pure 200 Å 5 µm 10 g10404000-001 Spherical Silica Gel, Premium Pure 200 Å 10 µm 10 g10502000-001 Spherical Silica Gel, Premium Pure 300 Å 3 µm 10 g10503000-001 Spherical Silica Gel, Premium Pure 300 Å 5 µm 10 g10504000-001 Spherical Silica Gel, Premium Pure 300 Å 10 µm 10 g


72200-001 Spherical Silica Gel, Premium Rf 70 Å 5 µm 10 g72300-001 Spherical Silica Gel, Premium Rf 70 Å 10 µm 10 g72400-001 Spherical Silica Gel, Premium Rf 70 Å 15 µm 10 g73200-001 Spherical Silica Gel, Premium Rf 100 Å 5 µm 10 g73300-001 Spherical Silica Gel, Premium Rf 100 Å 10 µm 10 g73400-001 Spherical Silica Gel, Premium Rf 100 Å 15 µm 10 g74200-001 Spherical Silica Gel, Premium Rf 150 Å 5 µm 10 g74300-001 Spherical Silica Gel, Premium Rf 150 Å 10 µm 10 g74400-001 Spherical Silica Gel, Premium Rf 150 Å 15 µm 10 g75200-001 Spherical Silica Gel, Premium Rf 200 Å 5 µm 10 g75300-001 Spherical Silica Gel, Premium Rf 200 Å 10 µm 10 g75400-001 Spherical Silica Gel, Premium Rf 200 Å 15 µm 10 g76200-001 Spherical Silica Gel, Premium Rf 300 Å 5 µm 10 g76300-001 Spherical Silica Gel, Premium Rf 300 Å 10 µm 10 g76400-001 Spherical Silica Gel, Premium Rf 300 Å 15 µm 10 g77200-001 Spherical Silica Gel, Premium Rf 500 Å 5 µm 10 g77300-001 Spherical Silica Gel, Premium Rf 500 Å 10 µm 10 g77400-001 Spherical Silica Gel, Premium Rf 500 Å 15 µm 10 g78200-001 Spherical Silica Gel, Premium Rf 800 Å 5 µm 10 g78300-001 Spherical Silica Gel, Premium Rf 800 Å 10 µm 10 g78400-001 Spherical Silica Gel, Premium Rf 800 Å 15 µm 10 g79200-001 Spherical Silica Gel, Premium Rf 1000 Å 5 µm 10 g79300-001 Spherical Silica Gel, Premium Rf 1000 Å 10 µm 10 g79400-001 Spherical Silica Gel, Premium Rf 1000 Å 15 µm 10 g



HPLC Silica Gels Spherical


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Sorbtech Medium Pressure Liquid Chromatography silica gels are high purity silica gels characterized by low metal ion content and lowsalt concentrations. The particle distribution is 20-45μm, and porosities of: 70Å, 100Å, 150Å, 200Å, 300Å, 500Å, 800Å, and 1000Å. These silica gels can be used as the base materials for functionalization (eg. C18, C8, Amino, etc.)


72600-05 Spherical Silica Gel, Premium Rf 70 Å 20 - 45 µm 500 g72600-1 Spherical Silica Gel, Premium Rf 70 Å 20 - 45 µm 1 kg72600-2.5 Spherical Silica Gel, Premium Rf 70 Å 20 - 45 µm 2.5 kg72600-5 Spherical Silica Gel, Premium Rf 70 Å 20 - 45 µm 5 kg72600-20 Spherical Silica Gel, Premium Rf 70 Å 20 - 45 µm 20 kg73600-05 Spherical Silica Gel, Premium Rf 100 Å 20 - 45 µm 500 g73600-1 Spherical Silica Gel, Premium Rf 100 Å 20 - 45 µm 1 kg74600-05 Spherical Silica Gel, Premium Rf 150 Å 20 - 45 µm 500 g74600-1 Spherical Silica Gel, Premium Rf 150 Å 20 - 45 µm 1 kg75600-05 Spherical Silica Gel, Premium Rf 200 Å 20 - 45 µm 500 g75600-1 Spherical Silica Gel, Premium Rf 200 Å 20 - 45 µm 1 kg76600-05 Spherical Silica Gel, Premium Rf 300 Å 20 - 45 µm 500 g76600-1 Spherical Silica Gel, Premium Rf 300 Å 20 - 45 µm 1 kg77600-05 Spherical Silica Gel, Premium Rf 500 Å 20 - 45 µm 500 g77600-1 Spherical Silica Gel, Premium Rf 500 Å 20 - 45 µm 1 kg78600-05 Spherical Silica Gel, Premium Rf 800 Å 20 - 45 µm 500 g78600-1 Spherical Silica Gel, Premium Rf 800 Å 20 - 45 µm 1 kg79600-05 Spherical Silica Gel, Premium Rf 1000 Å 20 - 45 µm 500 g79600-1 Spherical Silica Gel, Premium Rf 1000 Å 20 - 45 µm 1 kg


Our flash and gravity grade silica gels are ideal for flash chromatography, industrial scale LC, and batch adsorption applications. Sorbtech's flash and gravity silica gels function through hydrophilic interactions with more polar compounds generally retained longer, making them ideally suited for the purification of synthetic intermediates, oils, fats, and natural products (vitamins, flavor, fragrances, etc.). Available in 70Å, 100Å, 150Å, 200Å, 300Å, 500Å, 800Å, and 1000Å.


62500-05 Spherical Silica Gel, Premium Rf 70 Å 40-75 μm (200 x 400 mesh) 500 g62500-1 Spherical Silica Gel, Premium Rf 70 Å 40-75 μm (200 x 400 mesh) 1 kg62500-2.5 Spherical Silica Gel, Premium Rf 70 Å 40-75 μm (200 x 400 mesh) 2.5 kg62500-5 Spherical Silica Gel, Premium Rf 70 Å 40-75 μm (200 x 400 mesh) 5 kg62500-20 Spherical Silica Gel, Premium Rf 70 Å 40-75 μm (200 x 400 mesh) 20 kg62700-05 Spherical Silica Gel, Premium Rf 70 Å 75-200 μm (70 x 200 mesh) 500 g62700-1 Spherical Silica Gel, Premium Rf 70 Å 75-200 μm (70 x 200 mesh) 1 kg62700-2.5 Spherical Silica Gel, Premium Rf 70 Å 75-200 μm (70 x 200 mesh) 2.5 kg62700-5 Spherical Silica Gel, Premium Rf 70 Å 75-200 μm (70 x 200 mesh) 5 kg62700-20 Spherical Silica Gel, Premium Rf 70 Å 75-200 μm (70 x 200 mesh) 20 kg63500-05 Spherical Silica Gel, Premium Rf 100 Å 40-75 μm (200 x 400 mesh) 500 g63500-01 Spherical Silica Gel, Premium Rf 100 Å 40-75 μm (200 x 400 mesh) 1 kg63700-05 Spherical Silica Gel, Premium Rf 100 Å 75-200 μm (70 x 200 mesh) 500 g66500-05 Spherical Silica Gel, Premium Rf 300 Å 40-75 μm (200 x 400 mesh) 500 g66700-05 Spherical Silica Gel, Premium Rf 300 Å 75-200 μm (70 x 200 mesh) 500 g67500-05 Spherical Silica Gel, Premium Rf 500 Å 40-75 μm (200 x 400 mesh) 500 g


MPLC Silica Gels

Flash & Gravity Silica Gels

Spherical

Spherical

15Sorbtech


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The many combinations of porosities and particle sizes with the various surface modification options result in thousands of possible product choices. This allows you to tailor your decision process by matching a base silica gel with a desired modification. The end result is a functionalized silica gel specifically matched for optimizing separation.

Our Premium Pure™ grade is excellent for ultra-high purity needs such as a packing phase for HPLC columns. The elimination of metals during the silica gel manufacturing process provides an exceptional and homogeneous base material for the modification reaction. The surface bonding of the alkylsilanes, and the endcapping techniques, results in a silica gel with the lowest silanol activity.

The Premium Rf™ Grade is a superior choice for prep-HPLC, flash chromatography columns, and classic column chromatography.

The spherical particles serve as a strong backbone and outperform the more fragile granular-shaped competition. Additionally, the large number of pores within the desired diameter range results in high loading.

All of the functionalized silica gels show a very long life time, ie. reproducible results over a large number of separation and regeneration cycles.

Scaling up from analytical scale to industrial scale is also a benefit for using Sorbtech's silica gels. The variability of using different stationary phases from multiple manufacturers during the scale up process can be avoided.

From reversed phases to polar phases to ion exchange phases, let Sorbtech help you in choosing a functionalized silica gel to optimize your chromatographic separation.

Functionalized Silica Gel Bonded Phases


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Reversed PhasesReversed phase materials are compatible with aqueous media and have a lipophilic (hydrophobic) surfaces. They are mostly used for the separation of polar substances that are insoluble in purely organic solvents.

Therefore, we offer the following C18 options:C18 – Standard Bonding with endcapping. Excellent for routine analysis.C18AQ - Stable in 100% aqueous mobile phase systems.C18AQII – Offers 100% aqueous stability designed to match the retention times with leading aqueous C18 brands.C18DE – Dual Endcapping allows for enhanced pH stability 1 – 12.C18THE – High Endcapping and most hydrophobic.

C18 is the most popular and versatile reversed phase. It is capable of hydrophobic interactions with a wide variety of organic compounds and used for reversed phase chromatography for separation of nonpolar to polar compounds such as: fatty acids, glycerides, polycyclic aromatics, esters (phthalates), fat-soluble vitamins, steroids, prostaglandins, PTH amino acids.

C18 has become so popular because it is very useful for working with polar analytes – particularly those in biological samples.

Over the last few decades, a process called endcapping has become increasingly widespread. After the initial reaction of silanols on the silica surface to produce a lipophilic (hydrophobic) C18 surface, it is possible to perform endcapping – a second reaction – to eliminate any and all remaining silanols. This second reaction gives us the possibility to have different types of functional groups exposed to the mobile phase and analytes. This will modify the chemical characteristics of the surface chemistry as well as making the surface chemistry more reliable and useful.

The most common endcapping is to further alkylate the surface. It is also possible to have a second functional group which is very water soluble – such as an alcohol – which makes the totality of the C18 surface more hydrophilic.

C18 Silica Gel (Octadecyl Phase)

C18

C18Aqueous

C18Aqueous II

C18endcapped

C18Dual

endcapped

Increasing Hydrophobicity

Low HydrophobicityHigh Hydrophilicity

High HydrophobicityLow Hydrophilicity

C18Tri-functional

High endcapped

17Sorbtech


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HPLC C18 Silica Premium Pure

HPLC C18 Silica Premium Rf


10301546-001 C18 Spherical Silica Gel, Premium Pure, Endcapped 100 Å 2 μm 10 g10302046-001 C18 Spherical Silica Gel, Premium Pure, Endcapped 100 Å 3 μm 10 g10303046-001 C18 Spherical Silica Gel, Premium Pure, Endcapped 100 Å 5 μm 10 g10303047-001 C18 Spherical Silica Gel, Premium Pure, Dual Endcapped 100 Å 5 μm 10 g10303048-001 C18 Spherical Silica Gel, Premium Pure, Aqueous 100 Å 5 μm 10 g10304046-001 C18 Spherical Silica Gel, Premium Pure, Endcapped 100 Å 10 μm 10 g10304047-001 C18 Spherical Silica Gel, Premium Pure, Dual Endcapped 100 Å 10 μm 10 g10352046-001 C18 Spherical Silica Gel, Premium Pure, Endcapped 150 Å 3 μm 10 g10353046-001 C18 Spherical Silica Gel, Premium Pure, Endcapped 150 Å 5 μm 10 g10354046-001 C18 Spherical Silica Gel, Premium Pure, Endcapped 150 Å 10 μm 10 g10502046-001 C18 Spherical Silica Gel, Premium Pure, Endcapped 150 Å 3 μm 10 g10503046-001 C18 Spherical Silica Gel, Premium Pure, Endcapped 150 Å 5 μm 10 g10504046-001 C18 Spherical Silica Gel, Premium Pure, Endcapped 150 Å 10 μm 10 g


73246-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 5 µm 10 g73346-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 10 µm 10 g73446-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 15 µm 10 g74246-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 150 Å 5 µm 10 g74346-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 150 Å 10 µm 10 g74446-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 150 Å 15 µm 10 g75246-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 200 Å 5 µm 10 g75346-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 200 Å 10 µm 10 g75446-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 200 Å 15 µm 10 g76246-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 5 µm 10 g76346-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 10 µm 10 g76446-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 15 µm 10 g77246-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 5 µm 10 g77346-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 10 µm 10 g77446-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 15 µm 10 g78246-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 5 µm 10 g78346-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 10 µm 10 g78446-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 15 µm 10 g79246-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 5 µm 10 g79346-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 10 µm 10 g79446-001 C18 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 15 µm 10 g




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MPLC C18 Silica

Flash and Gravity C18 Silica


73646-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 20 - 45 μm 100 g73647-01 C18 Spherical Silica Gel, Premium Rf, Dual Endcapped 100 Å 20 - 45 μm 100 g73648-01 C18 Spherical Silica Gel, Premium Rf, Aqueous 100 Å 20 - 45 μm 100 g73652-01 C18 Spherical Silica Gel, Trifunctional, High-Endcapped 100 Å 20 - 45 μm 100 g74646-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 150 Å 20 - 45 μm 100 g75646-01 C18 Spherical Silica Gel, Premium Pure, Endcapped 200 Å 20 - 45 μm 100 g76646-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 20 - 45 μm 100 g77646-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 20 - 45 μm 100 g78646-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 20 - 45 μm 100 g79646-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 20 - 45 μm 100 g


452546M-01 C18 Granular Silica Gel, Endcapped 60 Å 40 - 63 μm 100 g452546M-05 C18 Granular Silica Gel, Endcapped 60 Å 40 - 63 μm 500 g452546M-1 C18 Granular Silica Gel, Endcapped 60 Å 40 - 63 μm 1 kg62546-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 70 Å 40 - 75 μm 100 g453546-1 C18 Granular Silica Gel, Endcapped 90 Å 40 - 63 μm 1 kg62746-1 C18 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 75 - 200 μm 1 kg

63546-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 40 - 75 μm 100 g63547-01 C18DE Spherical Silica Gel, Premium Rf, Dual Endcapped 100 Å 40 - 75 μm 100 g63548-01 C18AQ Spherical Silica Gel, Premium Rf, Aqueous 100 Å 40 - 75 μm 100 g63549-01 C18AQII Spherical Silica Gel, Premium Rf, Aqueous II 100 Å 40 - 75 μm 100 g63746-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 75 - 200 μm 100 g66546-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 40 - 75 μm 100 g66746-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 75 - 200 μm 100 g67546-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 40 - 75 μm 100 g67746-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 75 - 200 μm 100 g68546-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 40 - 75 μm 100 g68746-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 75 - 200 μm 100 g69546-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 40 - 75 μm 100 g69746-01 C18 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 75 - 200 μm 100 g



19Sorbtech


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Used for reversed phase chromatography for separation of moderately to highly polar compounds such as: steroids, nucleosides, cyclodextrins, pharmacological plant constituents. C8 bonded phases are similar to C18. The shorter alkyl chains cause slightly more polar secondary interactions with polar compounds.We offer the following C8 options:

C8 – Standard bonding with endcapping. Excellent for routine analysis.C8AQ - Stable in 100% aqueous mobile phase systems.C8AQII – Offers 100% aqueous stability designed to match the retention times with leading aqueous C18 brands.C8DE – Dual endcapping allows for enhanced pH stability 1 – 12.C8THE – High endcapping and most hydrophobic.

C8 Silica Gel (Octyl Phase)

HPLC C8 Silica Premium PureCAT. # DESCRIPTION POROSITY PARTICLE DIST. SIZE

10301562-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 100 Å 2 μm 10 g10302026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 100 Å 3 μm 10 g10303026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 100 Å 5 μm 10 g10304026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 100 Å 10 μm 10 g10304027-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 100 Å 10 μm 10 g10352026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 150 Å 3 μm 10 g10353026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 150 Å 5 μm 10 g10354026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 150 Å 10 μm 10 g10402026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 200 Å 3 μm 10 g10403026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 200 Å 5 μm 10 g10404026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 200 Å 10 μm 10 g10502026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 300 Å 3 μm 10 g10503026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 300 Å 5 μm 10 g10504026-001 C8 Spherical Silica Gel, Premium Pure, Endcapped 300 Å 10 μm 10 g



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HPLC C8 Silica Premium Rf


73226-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 5 μm 10 g73326-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 10 μm 10 g73426-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 15 μm 10 g74226-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 150 Å 5 μm 10 g74326-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 150 Å 10 μm 10 g74426-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 150 Å 15 μm 10 g75226-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 200 Å 5 μm 10 g75326-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 200 Å 10 μm 10 g75426-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 200 Å 15 μm 10 g76226-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 5 μm 10 g76326-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 10 μm 10 g76426-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 15 μm 10 g77226-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 5 μm 10 g77326-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 10 μm 10 g77426-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 15 μm 10 g78226-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 5 μm 10 g78326-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 10 μm 10 g78426-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 15 μm 10 g79226-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 5 μm 10 g79326-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 10 μm 10 g79426-001 C8 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 15 μm 10 g


MPLC C8 SilicaCAT. # DESCRIPTION POROSITY PARTICLE DIST. SIZE

73626-1 C8 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 20 - 45 μm 1 kg74626-1 C8 Spherical Silica Gel, Premium Rf, Endcapped 150 Å 20 - 45 μm 1 kg75626-1 C8 Spherical Silica Gel, Premium Rf, Endcapped 200 Å 20 - 45 μm 1 kg76626-1 C8 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 20 - 45 μm 1 kg77626-1 C8 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 20 - 45 μm 1 kg78626-1 C8 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 20 - 45 μm 1 kg79626-1 C8 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 20 - 45 μm 1 kg


C8 Flash and Gravity Silica


63526-01 C8 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 40 - 75 μm 100 g63726-01 C8 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 75 - 200 μm 100 g66526-01 C8 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 40 - 75 μm 100 g66726-01 C8 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 75 - 200 μm 100 g67526-01 C8 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 40 - 75 μm 100 g67726-01 C8 Spherical Silica Gel, Premium Rf, Endcapped 500 Å 75 - 200 μm 100 g68526-01 C8 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 40 - 75 μm 100 g68726-01 C8 Spherical Silica Gel, Premium Rf, Endcapped 800 Å 75 - 200 μm 100 g69526-01 C8 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 40 - 75 μm 100 g69726-01 C8 Spherical Silica Gel, Premium Rf, Endcapped 1000 Å 75 - 200 μm 100 g


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10302016-001 C4 Spherical Silica Gel, Premium Pure, Endcapped 100 Å 3 μm 10 g10303016-001 C4 Spherical Silica Gel, Premium Pure, Endcapped 150 Å 5 μm 10 g

63516-01 C4 Spherical Silica Gel, Premium Rf, Endcapped 200 Å 40 - 75 μm 100 g63716-01 C4 Spherical Silica Gel, Premium Rf, Endcapped 300 Å 75 - 200 μm 100 g

10502016-001 C4 Spherical Silica Gel, Premium Pure, Endcapped 500 Å 3 μm 10 g10503016-005 C4 Spherical Silica Gel, Premium Pure, Endcapped 800 Å 5 μm 50 g


63505-01 C2 Spherical Silica Gel, Premium Rf, Endcapped 100 Å 40 - 75 μm 100 g

Used for reversed phase chromatography for separation of hydrophobic substances. Retention times are generally longer than for C2 but shorter than C8 and C18 phases. C4 bonded phases are more polar than C18 and C8 because they have shorter alkyl chains and the silica surface with silyl ethers is still not completely shielded from contact with mobile phase or the analytes. Used for compounds that are highly retained on C18 or C8.

C2 is the least lipophilic of reversed phase materials. Retention times are generally much shorter than other reversed phases. C2 bonded phases are the most polar of the reversed phase adsorbents because they have the shortest alkyl chains (methyl groups) and the silica surface with silyl ethers and residual silanols is not completely shielded from contact with mobile phase or the analytes. Very often C2 bonded phases are used for analytes that have too much retention with the other reversed phase adsorbents. Used for plasma, urine, and aqueous samples.

C4 Silica Gel (Butyl Phase)

C2 Silica Gel (Dimethyl Phase)


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Polar Phases

Amino (NH2 Phase)

Cyano (CN Phase)

Phenyl

Will separate a variety of sugars with acetonitrile / water mobile phase. Alternatively with hexane / ethyl acetate mobile phase, it will function as a "less active" normal phase. In addition, since the surface contains primary amino groups, this phase can be used as a base for coupling chiral ligands.


10302055-001 NH2 Spherical Silica Gel, Premium Pure 100 Å 3 μm 10 g


63554-01 NH2 Spherical Silica Gel, Premium Rf 100 Å 40 - 75 μm 100 g


66555-01 NH2 Spherical Silica Gel, Premium Rf 300 Å 40 - 75 μm 100 g

Due to the highly polar nature of the -CΞN terminal group, this phase can be used in the Normal Phase Mode (replacing silica gel) with organic solvents and in the Reversed Phase Mode using water/methanol or water/acetonitrile solvents. Recommended Applications: Cyclosporins from serum/blood, Antidepressants from serum, Benzalkonium Chloride from aqueous solution, Betablockers from serum, Carbohydrates from aqueous solution, Narcotics from plasma, Herbicides from water.


10302060-001 CN Spherical Silica Gel, Premium Pure 100 Å 3 μm 10 g10303060-001 CN Spherical Silica Gel, Premium Pure 100 Å 5 μm 10 g

73460-001 CN Spherical Silica Gel, Premium Rf 100 Å 15 μm 10 g73660-01 CN Spherical Silica Gel, Premium Rf 100 Å 20 - 45 μm 100 g63560-01 CN Spherical Silica Gel, Premium Rf 100 Å 40 - 75 μm 100 g

The Phenyl terminal group bonded phase has sp2 character and is frequently used when main differences between the analytes involves π-π interactions. Recommended Applications: Afatoxins from food, caffeine from beverages, flavors, Phenols from water, trace elements from water.


10302065-001 Phenyl Spherical Silica Gel, Premium Pure 100 Å 3 μm 10 g10303065-001 Phenyl Spherical Silica Gel, Premium Pure 100 Å 5 μm 10 g

73465-001 Phenyl Spherical Silica Gel, Premium Rf 100 Å 15 μm 10 g73665-01 Phenyl Spherical Silica Gel, Premium Rf 100 Å 20 - 45 μm 100 g63566-01 Phenyl Spherical Silica Gel, Premium Rf 100 Å 40 - 75 μm 100 g63765-01 Phenyl Spherical Silica Gel, Premium Rf 100 Å 75 - 200 μm 100 g

DiolDiol bonded phases are particularly useful for aqueous GPC applications. As the Amino, and Cyano bonded phases, Diol adsorbent can be used in the normal phase mode with organic solvents. Recommended applications: food additives from beverages, alkaloids from plants, antibiotics from ointments, pesticides, and prostaglandins.


10302070-001 Diol Spherical Silica Gel, Premium Pure 100 Å 3 μm 10 g10303070-001 Diol Spherical Silica Gel, Premium Pure 100 Å 5 μm 10 g

63570-01 Diol Spherical Silica Gel, Premium Rf 100 Å 40 - 75 μm 100 g63770-01 Diol Spherical Silica Gel, Premium Rf 100 Å 75 - 200 μm 100 g

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Ion Exchange PhasesAnions are negatively charged. Cations are positively charged. Positively charged substances interact with negatively charged adsorbents. Negatively charged substances interact with positively charged adsorbents.

Support Primary, Secondary, and Teriary Amine Quaternary Amine Carboxylic Acid Sulfonic Acid

Common Designation WAX SAX WCX SCX

Charge onAdsorbent Surface

pH dependent positive charge

pH independentpositive charge

pH dependent negative charge

pH dependent negative charge

Type of Exchange Weak Anion Strong Anion Weak Cation Strong Cation

Useful pH Range 5 - 9 0 - 14 5 - 19 0 - 14

Charge of Analytes Negative Negative Positive Positive

(or Weak Anion Exchanger)PEI – or Poly (ethyleneimine) – is a polymer with repeating unit composed of the amine group and two carbon aliphatic CH2CH2 spacer.bPEI contains primary, secondary and tertiary amino groups but no quaternary amines and is therefore a good anion exchanger though it’s ion exchange capabilities are pH dependent.

WAX (PEI)


63595-01 WAX (PEI) Spherical Silica Gel, Premium Rf 100 Å 40 - 75 μm 100 g



(or Strong Anion Exchanger)A Strong Anion Exchanger is a cation on the surface of the adsorbent that bears a permanent positive charge under the conditions of use. The best functional group for this is a Quaternary Amine.

SAX (QAE)


63596-01 SAX (QAE) Spherical Silica Gel, Premium Rf 100 Å 40 - 75 μm 100 g



(Or Weak Cation Exchanger)The carboxyl group (-COOH) has a pKa of about 4 to 4.5and can be either neutral or negatively charged depending on the pH.

WCX (COOH)


63577-01 WCX (COOH) Spherical Silica Gel, Premium Rf 100 Å 40 - 75 μm 100 g




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(Propyl Sulfonic Acid (HSO3), strong cation exchanger.)The sulfonic acid group (-SO3H) has a pKa of -1 to -3 is always completely dissociated in the presence of water and therefore always negatively charged under the conditions of use.


63580-01 SCX (SO3H) Spherical Silica Gel, Premium Rf 100 Å 40 - 75 μm 100 g63780-01 SCX (SO3H) Spherical Silica Gel, Premium Rf 100 Å 75 - 200 μm 100 g66580-01 SCX (SO3H) Spherical Silica Gel, Premium Rf 300 Å 40 - 75 μm 100 g

SCX (SO3H)

Silica Gel Thiol (SH) is a silica gel with a surface functionalization of thiol groups (S-H); that is, silica gel surface-modified with 1-propane thiol. This material is an effective adsorbent material and scavenger for electrophiles and toxic heavy metals. Electrophiles that are adsorbed include alkyl, benzyl, and allyl halides as well as a variety of acid chlorides and isocyanates. Heavy metals, which are scavenged include Mercury (Hg), Lead (Pb), Cadmium (Cd), Palladium (Pd), Platinum (Pt), Copper (Cu), and Silver (Ag) and others. A wide range of operating conditions are possible.


40930M85-01 Thiol (SH) Spherical Silica Gel, Premium Rf 60 Å 40 - 63 μm 100 g40930M85-1 Thiol (SH) Spherical Silica Gel, Premium Rf 60 Å 40 - 63 μm 1 kg

40930M85-25 Thiol (SH) Spherical Silica Gel, Premium Rf 60 Å 40 - 63 μm 25 kg

Thiol (SH)

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Dry Colum Chromatography (DCC) is a fast, easy, and efficient method for separating and/or purifying preperative quantities of compounds. DCC links analytical Thin Layer Chromatography (TLC) with preparative column chromatography (CC). Like TLC, DCC is a “non-elution” method utilizing nylon foil tubing as a support and is available with UV-254 indicator. In TLC solvent flow is by capillary action going against gravity. However in DCC, solvent flow is by gravity. Columns of any size may be filled in DCC to separate or clean up very large quantities of materials. Both silica and alumina sorbents are available for DCC. Nylon foil tubing is solvent resistant and available in 1” - 6” diameters.Go green with less solvent use.

Product pH Activity Flourescent Indicator Bulk Density approx. Surface Area approx.

Silicafor DCC 7 III 0.5% 0.5 g/ml 500 - 600 m2/g

Alumina for DCC 7 III 0.5% 0.5 g/ml 200 m2/g

*Activity according to Brockmann

DCC EXPERIMENTDCC is a “non-elution” method of column chromatography, meaning, the separated substances remain in the column at the end of the chromatographic process. The use of nylon foil tubing allows for the removal of these separated substances by simply cutting the tubing into segments.

Dry Column Chromatography

Preparation of the ColumnThe nylon foil tubing comes in a roll of various widths in lengths of 100 ft. -- see table on next page. Cut the tubing to the desired length and allow an extra 10 cm for sealing the end. Close one end of the foil by sealing in the flame of a gas lighter, folding and stapling, tying up, or any other suitable method. To remove the folds of the foil, fill one side of the tubing with very hot water, rinse with acetone, and dry with hot air. Insert a pad of cotton or glass wool into the closed end of the tubing and pierce several times with a needle to allow air to escape while the solvent migrates through the column.Filling the Column

Preparation of the Sample

Carefully fill the tubing, adding small quantities at a time, with silica DCC, alumina DCC, or sorbent that has been conditioned with the solvent (mixture). After each small quantity filled, the column should be gently tapped on a hard surface or compacted with a vibrator. Upon filling completion, the column will be so rigid that it will hold itself upright and may be fixed with a simple laboratory clamp to a stand.

A few milliliters of a sample solution are added to about 1 g of the sorbent. (A larger amount of sorbent may be added to a larger sample solution with the practical ratio of the mixture to be separated (without solvent) to sorbent is normally about 1:5 (w/w)). The solvent is then evaporated using a rotation evaporator until sample and sorbent form a free flowing powder.

Charging the ColumnThe adsorbate of the sample is applied as a level layer on the top of the column. This layer is covered with about 2 cm of pure sorbent. If a concentrated sample solution is to be applied, care should be taken to achieve a starting zone, as narrow as possible. Applying liquid sample causes more uneven zones than applying the sample as an adsorbate. After penetration of the solution into the top of the column filling, about 2 cm of pure sorbent will be applied.

1

3

2

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14400-05 Silica for DCC, w/UV254, 60 Å 63-200 μm (70 x 230 mesh) 500 g14400-3 Silica for DCC, w/UV254, 60 Å 63-200 μm (70 x 230 mesh) 3 kg

14400-25 Silica for DCC, w/UV254, 60 Å 63-200 μm (70 x 230 mesh) 25 5g19500-05 Alumina for DCC, w/UV254 50-200 μm (70 x 270 mesh) 500 g19500-5 Alumina for DCC, w/UV254 50-200 μm (70 x 270 mesh) 5 kg

19500-50 Alumina for DCC, w/UV254 50-200 μm (70 x 270 mesh) 50 kgNT-1 Nylon Tubing for DCC - 1” x 100’

NT-1.5 Nylon Tubing for DCC - 1.5” x 100’NT-2 Nylon Tubing for DCC - 2” x 100’

NT-2.5 Nylon Tubing for DCC - 2.5” x 100’NT-6 Nylon Tubing for DCC - 6” x 100’

1

2 3 4 5

Development of the ColumnThe solvent (mixture) that formed the best results in the preliminary TLC experiments is carefully added to the top of the column by means of a separatory funnel. The solvent should migrate slowly and steadily through the column. A constant liquid head of about 1 - 2 cm gives the best results. A constant liquid head is attained by placing the end of the stem of the topside closed separatory funnel about 1 cm over the top of the column. Depending on the length of the column, the viscosity of the solvent (mixture), and the height of the liquid head, the development of the column should be finished within 15 to 30 minutes. As with TLC, the components of the initial sample mixture should be separated from each other on the column. The solvent volume applied to the top of the column shouldn’t exceed the amount which is necessary to fill the void fraction of the adsorbent bed.

4Recovery of the separated compoundsAfter completing development, the column is immediately placed flat on an appropriate cutting surface. The separated zones are marked on the column, using a UV light if necessary. The column is then cut into sections with a sharp knife. The column may be cut into small slices without sorbent crumbling. The cut slices are placed into Büchner funnels and extracted and processed by appropriate methods.

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Sorbent Technologies’ aluminas for column chromatography are the best available. A stringent manufacturing process yields standardized aluminas which are measured according to Brockmann methods. This ensures precise specifications and lot to lot reproducibility. Our aluminas are ideal for natural product extraction, purification and de-colorization, heavy metal removal, cleanup, and more. We offer the broadest selection of standardized aluminas with defined pH values (acid, base, neutral), specific activity levels (Super I, Activity I, II, III, IV, V) and numerous particle distributions (MPLC, Flash, Gravity). These aluminas can be adjusted to lower activity by the addition of polar media, preferably water. See deactivation procedure on next page.

Typical Applications for Aluminas

SUPER I

Standard Activity I Standard Activity I Standard Activity I

Food dyestuffsDetermination of morphineDetermination of biphenyl

Plant extractionFatty AcidsPlant waxesAzobenzene

AntibioticsEssential oilsPlant ExtractionEnzymesGlycosidesHormonesDiesel exhaustPharmaceutical compoundsFly agaric

AlkaloidsEssential oilsPlant ExtractionPurification of organic solventsInsecticidesClarification of fatty oilsExtract alcohol from chloroformExtract alcohol from chloroform

KetosteroidsGlycosidesVitaminsRemoval of pyrogensDehydration of orgranic solventsAlkaloids

Purification of orgranic solventsSynthetic basesDehydration of ethersRemoval of pyrogensNatural and synthetic dyestuffsHydrocarbons

SUPER I SUPER I

ACIDIC NEUTRAL BASIC

Alumina

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Deactivation allows the chromatographer the flexibility and freedom to deactivate alumina to the precise activity level required for the specific application. Alumina may be deactivated by the addition of polar media, preferably water, but also alcohols such as glycol or glycerol can be used. Sorbent Technologies’ standardized aluminas are based on a deactivation scale. Alumina Super I is standardized to such a high degree that all three surface types of Aluminas, (acid, basic, neutral) show an identical initial activity, and when deactivated with equal amounts of deactivator (e.g. water), they reach the same lower activity status. The percent of water addition is based upon weight/weight relationships. Activity level is according to Brockmann.Deactivation Procedure:

1. Weigh a known quantity of alumina and place into a tightly sealed vessel2. Add the required amount of water (by weight or volume)3. Shake the mixture in the closed vessel until all lumps disappear4. Allow the mixture to cool and equilibrate (preferably overnight)5. The container should remain tightly closed to retain activity level and can be stored for later use

Alumina pHapprox. Activity LOI

approx.Particle

Dist.

WaterSol.

Matterapprox.

Bulk Density approx.

Surface Area approx.

Super I - Acidic 4.5 Super I 1.3% 50-200 µm 0.1% 0.8 g/ml 200 m2/g

Super I - Neutral 7.5 Super I 1% 50-200 µm 0.1% 0.8 g/ml 200 m2/g

Super I - Basic 10 Super I 1% 50-200 µm 0.1% 0.8 g/ml 200 m2/g

Standard Act. I - Acidic 4.5 Act. I 1.7% 50-200 µm 0.1% 0.8 g/ml 150 m2/g

Standard Act. I - Neutral 7.5 Act. I 1.7% 50-200 µm 0.1% 0.8 g/ml 150 m2/g

Standard Act. I - Basic 10 Act. I 1.7% 50-200 µm 0.1% 0.8 g/ml 150 m2/g

Act. II - III 10 Act. II - III 50-200 µm 0.8 g/ml

Alumina N for Flash Chromatography 7.4 32-63 µm 0.2% 0.8 g/ml 200 m2/gAlumina B - Super I for Dioxin Analysis 10 0.7% 50-200 µm 0.1% 0.8 g/ml 200 m2/g

Alumina R 4.3 50-200 µm 0.8 g/ml

Activity Super I I II III IV V Activity LevelAccording to Brockmann

Super I 0 1 4 7 10 19 % water to addStd. Act. I N/A 0 3 6 10 15 % water to add

Deactivation of Alumina

The amount of water needed for deactivation can be read off the graph below:


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Alumina Activity Super I is designed for the separation of polar samples in non-solvent systems and the purification and dehydration of solvents for HPLC and other analytical techniques. In a non-polar environment, Alumina Activity Super I shows sorption capacity approximately twice as high as that of Alumina Standard Activity I. Its initial activity (<1% moisture) is kept in a very narrow range and its deactivation is extremely constant.


15100-1 Alumina, Super I - Acidic 50-200 μm (70 x 270 mesh) 1 kg15100-5 Alumina, Super I - Acidic 50-200 μm (70 x 270 mesh) 5 kg15130-1 Alumina, Super I - Neutral 50-200 μm (70 x 270 mesh) 1 kg15130-5 Alumina, Super I - Neutral 50-200 μm (70 x 270 mesh) 5 kg15160-1 Alumina, Super I - Basic 50-200 μm (70 x 270 mesh) 1 kg15160-5 Alumina, Super I - Basic 50-200 μm (70 x 270 mesh) 5 kg

Alumina | Super I

Alumina, Act. II-III is an economical adsorbent of medium activity. Alumina, Act. II-III is often used when activated charcoal cannot be used due to its organic nature or where the cation exchange properties of basic alumina are desired. It is suitable for the development of separation methods and technical separations where a custom-made adsorbent is not available. Between 3 - 6% moisture.


15300-1 Alumina, Activity II-III 50-200 μm (70 x 270 mesh) 1 kg15300-5 Alumina, Activity II-III 50-200 μm (70 x 270 mesh) 5 kg

15300-50 Alumina, Activity II-III 50-200 μm (70 x 270 mesh) 50 kg

Alumina | Activity II-III

Alumina, Standard Activity I is similar to the Alumina, Activity Super I in that it is also standardized. The starting activity level is about half as high and can be deactivated according to the deactivation table on the previous page. Standardized manufacturing processes provide a specific Brockmann dryness level for lot to lot reproducibility and precise deactivation possibilities. Between 1 - 3% moisture.


15600-1 Alumina, Standard Act. I - Acidic 50-200 μm (70 x 270 mesh) 1 kg15600-5 Alumina, Standard Act. I - Acidic 50-200 μm (70 x 270 mesh) 5 kg

15600-25 Alumina, Standard Act. I - Acidic 50-200 μm (70 x 270 mesh) 25 kg15600-50 Alumina, Standard Act. I - Acidic 50-200 μm (70 x 270 mesh) 50 kg15740-1 Alumina, Standard Act. I - Neutral 50-200 μm (70 x 270 mesh) 1 kg15740-5 Alumina, Standard Act. I - Neutral 50-200 μm (70 x 270 mesh) 5 kg

15740-25 Alumina, Standard Act. I - Neutral 50-200 μm (70 x 270 mesh) 25 kg15740-50 Alumina, Standard Act. I - Neutral 50-200 μm (70 x 270 mesh) 50 kg15640-1 Alumina, Standard Act. I - Basic 50-200 μm (70 x 270 mesh) 1 kg15640-5 Alumina, Standard Act. I - Basic 50-200 μm (70 x 270 mesh) 5 kg

15640-25 Alumina, Standard Act. I - Basic 50-200 μm (70 x 270 mesh) 25 kg15460-50 Alumina, Standard Act. I - Basic 50-200 μm (70 x 270 mesh) 50 kg

Alumina | Standard Act. I


16050-1 Alumina, Neutral for "Flash" Chromatography 32-63 μm (230 x 450 mesh) 1 kg16050-5 Alumina, Neutral for "Flash" Chromatography 32-63 μm (230 x 450 mesh) 5 kg

16050-25 Alumina, Neutral for "Flash" Chromatography 32-63 μm (230 x 450 mesh) 25 kg19600-05 Alumina B - Super I for Dioxin Analysis 50-200 μm (70 x 270 mesh) 500 g19650-05 Alumina R for the Technique of Isotopes 50-200 μm (70 x 270 mesh) 500 g

Other AluminasAlumina, Neutral for “Flash” Chromatography has a finer particle distribution than the Super I and Act. I aluminas for sharper separations. Other finer cuts are also available. Alumina B – Super I for Dioxin Analysis was specially developed to meet the high recovery level required for the analysis of polychlorinated dibenzodioxins and dibenzofurans according to VDI-Regulation 3499, part I. Alumina R is an acidic alumina developed for use in isotope chemistry. It is used to produce technetium generators or technetium cows. Another example of its application is the determination of catecholamines.

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Solvent Purification Methods:UV-transparent solvents are essential for spectroscopy below 300 nm. Classical methods of purifying solvents for UV spectroscopy are very time-consuming and complex. This is not the case when using adsorption filtration. UV-transparent solvents can be quickly and easily prepared from solvents of ordinary analytical purity. Moreover, they are less sensitive to air and thus more stable. Purification of Hydrocarbon Solvents• A sample of 300 ml analytical grade n-hexane or pre-purified (by shaking with sulfuric acid to remove alkenes or

olefines) technical grade cyclohexane is additionally filtered without suction through a column (25 mm I.D. and 250 mm height) filled with 150 g Alumina Basic, Act. I (Cat.# 15640). The filtrate is anhydrous and can immediately be used.

• Technical grade cyclohexane, that is not pre-purified, can easily be purified by filtering through a combined Silica Gel, Active, 60 Å, 63-200 μm / Alumina Basic, Act. I column. A column (25 mm I.D. and 400 mm height) is first filled with 100 g Alumina Basic, Act. I (Cat.# 15640) and on top of this, 100g of Silica Gel, Active, 60 Å, 63-200 μm (Cat.# 10840) is added. Then the crude cyclohexane is dripped through. Purified cyclohexane is anhydrous.

Purification of Organic SolventsDepending on the kind of impurities, which depends on the origin and the nature of the solvent, the solvent can be filtered through a column filled with Silica Gel, Active, 60 Å, 63-200 μm, Alumina (Acid, Neutral or Basic), Act. I, or a combination of the two with the alumina section of the column above the silica gel section of the column. The following table gives recommendations on the amount and type of adsorbent to be used as well as the solvent yield and obtained purity.

Solvent

Alumina,Acid,Act. I

Cat.# 15600

Alumina,Neutral,

Act. ICat.# 15740

Alumina,Basic, Act. I

Cat.# 15640

Silica Gel,Active, 60

Å,63-200 µm

Cat.# 10840

SolventYield(ml)

50% Trans. at nm(4 cm

cuvette)

n-Pentane 40 g 250 217 40 g 55 217n-Hexane 40 g 250 218 40 g 50 Cyclohexane 40 g 700 233 40 g 80 234n-Heptane 40 g 500 224 40 g 70 232Isooctane 40 g 40 g 250 226 40 g 1600 224 40 g 255 255 40 g 255 255Carbon tetrachloride

40 g 80 280

40 g 4000 276Chloroform DAB6 40 g 340 40 g 100 Nitromethane 40 g 40 402Dimethyl sulfoxide

40 g 170 326

Pyridine 40 g 30 321 40 g 35 332 40 g 30 20 g 20 g 25 316

Silica Gel, Active,

60 Å 63-200 μmAlumina,

Basic, Act. I, 50-200 μm

Silica Gel, Active,

60 Å, 63-200 μm

Alumina, (Acid, Neutral or Basic)Act. I, 50-200 μm

Silica Gel, Active,60 Å, 63-200 μm

Alumina, (Acid, Neutral or Basic)Act. I, 50-200 μm

+

G. Hesse and H. Schildknecht, Angew. Chem. 67, 737 (1955) G. Hesse, B. P. Engelbrecht, H. Engelbrecht and S. Nitsch, Z. Anal. Chem. 241, 91 (1968)

Alumina Solvent Purification


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Alumina for large scale purification is a granular activated alumina that has a high surface area and a large pore volume. Because of these physical characteristics it has a wide variety of applications. Powders can be manufactured to meet specific customer needs and packaged in bulk super-sack sizes. Alumina for large scale purification can adsorb a great number of pollutants from liquids and vapors. Flocculation adsorption can also occur depending on the pH-value. The degree of adsorption depends on the polarity substance to be adsorbed; polar substances are bound stronger than non-polar ones. In mixtures of substances with differing polarity, a chromatographic separation will take place.

Cat No. Description Part. Dist. Size85800-100 Activated Alumina for Water/Process Liquids Clean-up < 0.5 mm 100 kg

85801-100 Activated Alumina for Water/Process Liquids Clean-up 0.3 - 1 mm 100 kg

85813-100 Activated Alumina for Purification 1 - 3 mm 100 kg

85825-100 Activated Alumina for Purification 2 - 5 mm 100 kg

Specific surface area (BET): 230-300 m2/gLoss on ignition: ~ 9%Pore volume: ~ 0.35 cm3/gChemical analysis: Al2O3 ~ 90% Na2O total 0.3 - 0.5% CaO 0.01 - 0.03% Fe2O3 0.01 - 0.03% SiO2 0.01 - 0.03% TiO2 ~ 0.005%

Activated Alumina for Large Scale Purification

• Drying and purification of organic liquids and gases• Elimination of pollutants from circulating reaction mixtures• Elimination of system-damaging substances in circulation loops• Selective purification of raw materials• Concentration of highly diluted product or by-product solutions for recycling• Adsorption of heavy metals (soluble compounds) in underground and drinking water

- Fluorides - Phosphates - Arsenic acids - Humic acids• Purification of processing liquids, industrial water, and waste water

- Fluorides - Phosphates - Arsenic acids - Acidic phenols - Amines - Ionic detergents - Lignosulfonic acids - Chlorine lignine - Vapor condensates - Organic acids - Acidic / basic dyes - Dyestuffs waste water - Bleach waste water (pulp industry)• Purification and drying of exhaust air and gases

- Fluorine - Hydrogen fluoride - Odors (in testing phase)

Typical Applications

Procedure

Typical Technical Data

RegenerationReduction of waste levels with alumina for large scale purification is possible in every kind of adsorption reactor. Sufficient contact time between the adsorbent and the medium to be purified must be ensured. Process conditions must be defined individually. When using common fixed-bed reactors, approximate contact times of 0.5 - 0.75 hours and filtration rates of 1-2 m/hr can be used in the installation design. Optimal adsorption levels of waste and by-products can then be attained.

In most application areas, regeneration of saturated alumina is not only desirable, but possible. When designing a continuous adsorption plant, the reaction design should reflect the cycling of the saturated adsorbent in sequence with the regeneration phase. The total adsorption capacity should be calculated to assure optimal adsorption. The design of the regeneration procedure depends on both the nature of the adsorbed substances and on their recyclability.

Thermal treatment (up to a maximum of 600° C) can remove organic adsorbed impurities during regeneration without losing alumina activity. By adjusting the pH inorganic adsorbed impurities, such as phosphates or fluorides can be eluted chemically. After recycling by one of the regeneration processes, the alumina is ready for use again. Based on previous experience, each regenerating cycle results in a 1-3% loss of alumina from abrasion. This loss must be compensated for when restarting each individual reactor.

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Process stream purificationRemoval of highly polar compounds such as TBC, alcohols, ethers, and others.

Acid removalOur alumina spheres remove the acids formed from the degradation of transformer oils, lubricating oils, and refrigerants. Also, they remove the residual halides and water during the manufacturing of chlorinated and/or fluorinated hydrocarbons, yielding a non-corrosive product.

DryingNearly all gases and liquids be dried with alumina. Water removal is often necessary for efficient processing, storage, transportation or usage of fluids. For example, water can form hydrates with many compounds including hydrocarbons, simulating ice that may plug equipment.

Sorbtech’s activated alumina spheres are very porous and have a high surface area, which allows adsorption of gases and liquids and do not swell. They will not soften or disintegrate when immersed in liquids. When used to adsorb water, they act as an efficient desiccant. Molecules with the highest polarity are preferentially adsorbed. In the absence of irreversible fouling, they may be regenerated to their original adsorption efficiency by heating to a temperature between 350-600° F (177-316° C). For the most efficient subsequent adsorption, high temperature, low pressure and dry inert gas regeneration is recommended.

Benefits

GasesAirAmmoniaCO2Chlorine

EthaneEthyleneFurnace gasHeliumHydrogen

Hydrogen chlorideHydrogen sulfideMethaneNatural gasNitrogen

OxygenPropanePropyleneRefrigerantsSulfur dioxide

LiquidsBenzeneButaneButeneButyl acetateCarbon tetrachlorideChlorobenzeneCyclohexane

Ethyl acetateGasolinesHeptaneHexaneHydraulic oilsJet fuel Kerosene

Lubricating oilsNapthaNitrobenzenePentanePipeline productsPropanePropylene

RefrigerantsStyreneTolueneTransformer oilVegetable oilXylene

Available in 1 kg, 5 kg, 25 kg, 50 kg, 100 kg and bulk super sack packaging

Cat No.Sphere

Size (English)

Sphere Size

(Metric)

Surface Area

Total pore volume

Packed bulk density

Crush strength

Abrasion loss wt%

Static sorption at

11% RH

Static sorption at

58% RH

Static sorption at

97% RH

92000 7 x 14 Tyler Mesh

2.0 mm 360 m2/g

0.5 cc/g 48 lb/ft3

(769 kg/m3)11 lb(5 kg)

0.1 8 22 42

92010 1/8” 3.2 mm 355 m2/g 0.5 cc/g 48 lb/ft3

(769 kg/m3)30 lb

(14 kg)0.1 8 22 42

92020 3/16” 4.8 mm 340 m2/g

0.5 cc/g 48 lb/ft3

(769 kg/m3)55 lb

(25 kg)0.1 7 21 40

92030 1/4” 6.4 mm 325 m2/g 0.5 cc/g 48 lb/ft3

(769 kg/m3)70 lb

(32 kg)0.1 7 19 38

Substances dried with alumina spheres

Alumina Spheres

Uniform ball size• Important in high pressure gas

dehydration for reduction of pressure drop

• Minimizes channeling• Yields higher efficiencies

1Low Abrasion• Ensures less dusting during

transport, loading, and service life

• Reduces downstream valve and filter plugging

3High Adsorptive Capacity• High surface area• Tailored pore distribution• Achieve an ultra low H2O effluent4

High Crush Strength• Allows rapid pneumatic loading • Enables use of tall towers• Effective for dehydrating acid

containing gases and liquids, ex: CO2• Longer operating life

2

Product Applications

Adsorption Isothermfor Alumina Spheres at 25° C

% Relative Humidity

Wt.

% H

20 A

dsor

bed


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Cat No. Description Part. Dist. Quantity26500-025 Polyamide for TLC 2-20 μm 250 g

26500-1 Polyamide for TLC 2-20 μm 1 kg

26520-025 Polyamide for Flash Chromatography <70 μm 250 g

26520-1 Polyamide for Flash Chromatography <70 μm 1 kg

26530-025 Polyamide for Classic Column Chromatography 50-160 μm 250 g

26530-1 Polyamide For Classic Column Chromatography 50-160 μm 1 kg

26530-5 Polyamide For Classic Column Chromatography 50-160 μm 5 kg

26540-1 Polyamide for Process 100-300 μm 1 kg

Polyamide is suited for separating natural substances with phenolic and polyphenolic groups. The chromatography of phenolic compounds on polyamide has been frequently applied for the isolation and structure determination of different natural products. Carboxylic acids are also bonded to polyamide via hydrogen bonds. The affinity for an aliphatic monocarboxylic acid is low. Dicarboxylic acids and aromatic acids are retained stronger, especially if the aromatic part of the molecule is larger.

Strong affinity for polyamide is found with numerous aromatic nitro compounds. The interaction between the nitro compound and polyamide corresponds to a reaction of a Lewis acid with a base. This is why separations of aromatic nitro compounds on polyamide are considered as ion exchange. For this reason elution in sharp bands requires an eluent with good buffering properties. This principle has been successfully applied for separating dinitrophenylamino acids. Quinones are irreversibly bonded to the polyamide due to the adsorbent.

Cat No. Description Part. Dist. Quantity09410-05 Florisil - A Grade, Activated at 650° C 16 x 30 mesh 500 g

09410-20 Florisil - A Grade, Activated at 650° C 16 x 30 mesh 20 kg

09415-05 Florisil - A Grade, Activated at 650° C 30 x 60 mesh 500 g






09435-05 Florisil - A Grade, Activated at 650° C -200 mesh 500 g

09435-20 Florisil - A Grade, Activated at 650° C -200 mesh 20 kg

09500-05 Florisil - Pesticide Residue Grade 60 x 100 mesh 500 g

09500-20 Florisil - Pesticide Residue Grade 60 x 100 mesh 20 kg

Bulk sizes are available

Florisil® is a very hard granular magnesia silica gel. MgO 15.5 ± 0.5% SiO2 84.0 ± 0.5% Na2SO4 ≤ 1.0%Applications include clean-up of pesticide residues, separation of chlorinated pesticides, isolation of steroids, sex hormones, antibiotics, and separation of lipids.

Polyamides exhibit constant selectivities towards:• Phenols, aromatic nitro and amino compounds • Chalcones, quinones, flavones• Carbonic acids and their amides • Anthraquinones• DNP-amino acids • Sulfonic acids and their amides

Specialty Adsorbents Polyamide

Florisil®

35Sorbtech


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• Composition: alkali alumino silicate ceramic• Shape: solid spheres• Color: white• High strength: crush strength > 4,200 kg/cm2 (> 60,000 psi)

For use in column affinity chromatography to isolate and study glycoprotein factors promoting attachment and spreading of cells in a culture.

Sorbtech silica desiccants are non-corrosive, non-toxic, and chemically inert. It's is a highly porous form of silica with an extremely large internal surface area and an affinity towards water and other polar compounds. It will not change size or shape, even when the silica gel is water saturated, it will remain free-flowing.

Silica desiccants are available with a blue (cobalt chloride) or yellow (phenol phthalein) indicator. Blue indicator spheres turn pink after reaching moisture capacity and yellow spheres turn blue-green. The yellow indicator spheres are non-hazardous, making them perfect for pharmaceutical and food products.

Cat No. Description Size Quantity22000-1 Silica Gel Desiccant Spheres 1-3 mm 1 kg

22000-5 Silica Gel Desiccant Spheres 1-3 mm 5 kg



22102-1 Silica Gel Desiccant Spheres w/ 20% Blue Indicator 1-3 mm 1 kg






23210-1 Silica Gel Desiccant Spheres w/100% Yellow Indicator 2-5 mm 1 kg

23210-5 Silica Gel Desiccant Spheres w/100% Yellow Indicator 2-5 mm 5 kg

23210-25 Silica Gel Desiccant Spheres w/100% Yellow Indicator 2-5 mm 25 kgBulk sizes are available

Cat No. Description Surface Area Density Quantity93903-01 Alumina Ceramic Beads, 1-12 μm (3 μm mean) 5 m2/g 1.5 g/cc 100 g

93904-01 Alumina Ceramic Beads, 1-24 μm (4 μm mean) 3 m2/g 1.5 g/cc 100 g

93910-01 Alumina Ceramic Boeads, 1-40 μm (10 μm mean) 3 m2/g 1.5 g/cc 100 gBulk sizes are available

Cat No. Description Density Quantity93015-01 Glass Beads, 9-24 μm (15 μm mean) 0.60 g/cc 100 g93018-01 Glass Beads, 10-30 μm (18 μm mean) 0.30 g/cc 100 g

93022-01 Glass Beads, 11-37 μm (22 μm mean) 0.42 g/cc 100 g



Bulk sizes are available

• Composition: soda-lime borosilicate glass• Shape: Hollow spheres with thin walls• Color: white, powdery

• Free flowing• High strength

Silica Desiccants

Glass Beads

Alumina Ceramic Beads

Adsorbents · 2020-02-21 · 5 Sorbtech. Premium Rf™ is the finest granular silica gel on the...

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