Adminsitrative Record Page 19287 - California State Water ...€¦ · 2.5 The compounds are...
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Caltest Analytical Laboratory
GCMS-NCI SIM / SW-846 8270 Method Validation Submittal
Confidential 11/22/2010
Analytical Method Standard Operating Procedure
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CALTEST STANDARD OPERATING PROCEDURE
PYRETHROIDS BY GCMS-NCI SIM / SW-846 8270 Modified
Reviewed by: Richard Heines Title: Organics Technical Lead Date: 11/23/10 Reviewed by: Sonya Babcock Title: Quality Assurance Officer Date: 11/23/10 Approved by: Patrick Ingram Title: Laboratory Director Date: 11/23/10 This SOP outlines the exact procedure to be followed by all staff of Caltest Laboratory who are performing the indicated method. It is the responsibility of any individual performing the procedure to follow these instructions outlined in this document. Any significant modifications to this method require an amendment to this SOP with the approval of the department Coordinator, Laboratory Director (LD) and QAO. All amendments must be identified below, and attached to this document to be considered valid changes. Any deviations from this SOP require prior authorization from the department Coordinator, Lab. Director and QAO. In addition, all deviations from the written procedure require complete documentation in the appropriate logbook.
Review Section Description of Changes Date Initials Coord., LD, QA
#1 _____ _______________________________________ _____/_____/_____ ____________
#2 _____ _______________________________________ _____/_____/_____ ____________
#3 _____ _______________________________________ _____/_____/_____ ____________
#4 _____ _______________________________________ _____/_____/_____ ____________ #5 _____ _______________________________________ _____/_____/_____ ____________
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PYRETHROIDS by NCI ANALYTICAL PROCEDURE 1. Scope and Application
1.1 This method is applicable for trace level determination of pyrethroids in water samples of low to
moderate complexity (surface waters, monitoring waters, wastewaters, et al) and solid matrices (soil, sediment, bio-solids, etc.).
1.2 Samples are extracted and undergo extensive cleanup measures prior to analysis by Gas Chromatography/Negative Chemical Ion-Mass Spectrometry-Selective Ion Monitoring (GCMS-NCI SIM) using the appropriate sample preparation procedures. All soils, sediments and sludges are dryed before extraction.
1.3 This method is specific to the determination of Bifenthrin, Cyfluthrin, Lambda-Cyhalothrin, Cypermethrin, Deltamethrin, Esfenvalerate, Fenpropathrin, and Permethrin as primary reportable target compounds with five additional optional pyrethroids and two organo-phosphate pesticides, but should be applicable to other pyrethroids and related pesticides as well. However, the selectivity of Chemical Ionization Mode limits the determination of compounds.
1.4 The detection limits of this method approaches instrumental limitations and may be affected by the level of sample interferences. The calibration range is targeted to be 0.5 ng/L to 5 ng/L for waters and 0.25 µg/Kg to 1 µg/Kg for solids, which typify the minimum quantity that can be detected with no interferences present.
1.5 This method is for use by or under the supervision of analysts experienced in the use and interpretation of Gas Chromatography/Negative Chemical Ion-Mass Spectrometry data.
2. Summary of Method
2.1 The analytical procedures of this method model the Robinson/Syngenta method, “Analytical Method for the Determination of Synthetic Pyrethroids in Sediment by Gas Chromatography-Negative Chemical Ionization Mass Spectrometry" (2006).
2.2 This method represents a modification of SW-846 method 8270 “Semivolatile Organic Compounds by Gas Chromatography/Mass Spectrometry (GC/MS)”.
2.3 This method is appropriate for extracts of all appropriate matrices prepared by EPA Methods 3510C, 3520C, 3535 3540C, 3541, 3550 (refer to appropriate SOP)..
2.3.1 Extraction of water samples are based on a nominal volume of 1000mL of sample extracted into a final volume of 1mL in Hexane, to achieve method detection limits;
2.3.2 Solids are based on a nominal sample amount of 15 grams of sample extracted into a final volume of 1mL in Hexane, to achieve method detection limits;
2.3.3 When actual sample amounts used differ from the nominal values stated, detection limits will be adjusted accordingly.
2.4 All soils/sludges and waters may necessitate extensive clean up to remove or reduce interferences before being analyzed (refer to clean up SOP DOC#: O-3-phase clean up).
2.5 The compounds are introduced into the GCMS by injecting the sample extract into a gas chromatograph (GC) with a narrow-bore fused-silica capillary column. The GC column is temperature-programmed to separate the analytes, which are then detected with a mass spectrometer (MS) connected to the gas chromatograph. The mass spectrometer is operated in the negative chemical ionization (NCI) and selective ion monitoring (SIM) modes in order to improve sensitivity and selectivity of the pyrethroid pesticides. If needed to possibly minimize matrix enhancements of the analytes the GC can be split to two detectors, a MS and micro Electron Capture Detector (µECD) in an 5:1 split ratio. The µECD signal can also be a better aid in determining the extent of the matrix background than the mass spectrometer run in the highly
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selective negative chemical ionization mode. In the dual detector mode, results could be reported from the µECD and confirmed by the MS, if all data meets method criteria.
2.6 Qualitative identification of the target analytes (Pyrethroid pesticides, Chlorpyrifos and Diazinon) on the MSD is accomplished by comparing their selective mass ion ratios to the mass ion ratios of an authentic standard. There are two techniques of quantitation that are used in this method. Either internal multi point ICAL quantitation or external two point bracketing standard quantitation.
2.6.1 The internal multi point ICAL quantitation technique is accomplished by comparing the response of a major (quantitation) ion relative to an internal standard using a five-point calibration curve.
2.6.2 The external two point bracketing standard quantitation technique is accomplished by comparing the response of a major (quantitation) ion relative to an external (no internal standard used) two point bracketing standard calibration curve. The external two point bracketing quantitation technique is useful when there are known matrix enhancements or detector response drift which is fairly common with GCMS when operated in the negative chemical ionization mode.
3. Definitions
The following terms are defined for use in this document:
3.1 ACCURACY: The closeness of agreement between an observed value and an accepted reference value. When applied to a set of observed values, accuracy will be a combination of a random component and of a common systematic error (or bias) component.
3.2 BATCH: A group of samples which behave similarly with respect to the sampling or the testing
procedures being employed and which are processed as a unit. An analytical batch is comprised of up to 20 environmental samples plus related QC samples.
3.3 CONTROL SAMPLE: A QC sample introduced into a process to monitor the performance of the
system. 3.4 FIELD DUPLICATES: Independent samples which are collected as close as possible to the same
point in space and time. They are two separate samples taken from the same source, stored in separate containers, and analyzed independently. These duplicates are useful in documenting the precision of the sampling process.
3.5 LABORATORY CONTROL SPIKE: A known matrix spiked with compound(s) representative of
sample target analytes. This is used to document laboratory performance.
3.6 MATRIX: The component or substrate (e.g., surface water, drinking water), which contains the analyte of interest.
3.7 MATRIX DUPLICATE: An intralaboratory-split sample, which is used to document the precision of
a method in a given sample matrix.
3.8 MATRIX SPIKE: An aliquot of sample spiked with a known concentration of target analyte(s). The spiking occurs prior to sample preparation and analysis. A matrix spike is used to document the bias of a method in a given sample matrix.
3.9 MATRIX SPIKE DUPLICATES: Intralaboratory split samples spiked with identical concentrations of
target analyte(s). The spiking occurs prior to sample preparation and analysis. They are used to document the precision and bias of a method in a given sample matrix.
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3.10 METHOD BLANK: An analyte-free matrix to which all reagents are added in the same volumes or proportions as used in sample processing. The method blank should be carried through the complete sample preparation and analytical procedure. The method blank is used to document contamination resulting from the analytical process.
3.11 PRECISION: The agreement among a set of replicate measurements without assumption of
knowledge of the true value. Precision is estimated by means of duplicate/replicate analyses. These samples should contain concentrations of analyte above the MDL, and may involve the use of matrix spikes. The most commonly used estimates of precision are: 3.11.1 Relative Standard Deviation (RSD) or the Coefficient of Variation (CV) measuring 2, or
more, sample replicates; RSD = CV = 100 S/x; where: x = the arithmetic mean of the measurements, and S = xi variance;
3.11.2 Relative Percent Difference (RPD) when only two sample replicates are available. RPD = 100 [(x1 - x2)/{( x1 + x2)/2}] where: x = sample concentration, respectively
3.12 REAGENT GRADE: Analytical reagent (AR) grade, ACS reagent grade, and reagent grade are
synonymous terms for reagents which conform to the current specifications of the Committee on Analytical Reagents of the American Chemical Society.
3.13 REAGENT WATER: Water that has been generated by any method, which would achieve the
performance specifications for ASTM Type II water. For organic analyses, see the definition of organic-free reagent water.
3.14 STANDARD CURVE: A plot of concentrations of known analyte standards versus the instrument
response to the analyte. Calibration standards are prepared by successively diluting a standard solution to produce working standards, which cover the working range of the instrument. Standards should be prepared at the frequency specified in the appropriate section. The calibration standards should be prepared using the same type of solvent and at the same concentration as will result in the samples following sample preparation. This is applicable to organic and inorganic chemical analyses.
3.15 SURROGATE: An organic compound which is similar to the target analyte(s) in chemical
composition and behavior in the analytical process, but which is not normally found in environmental samples.
3.16 SIM: Selective ion monitoring 3.17 PCI: Positive Chemical Ionization
3.18 NCI: Negative Chemical Ionization
3.19 MSD: Mass selective detector.
3.20 GCMS: Gas Chromatography Mass Spectrometry
3.21 ECD: Electron Capture Detector
3.22 SOURCE: Component of MSD where ionization and ion focusing take place
3.23 AMU: Atomic mass unit
4. Interferences
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4.1 Solvents, reagents, glassware and other hardware used during extraction and standards preparation can yield interferences. The lot numbers, or the Caltest reagent ID number or Caltest source number of all reagents and solvents used for standards preparation shall be recorded in the Standards Log Book to trace any sources of contamination that may occur.
4.2 Plastics should be avoided during the extract preparation procedure to eliminate sources of
phthalate esters. Care should also be taken to limit contact of the latex gloves with the sample or reagents. Only Teflon, glass or metal equipment are to be used, such as Teflon squirt bottles, and all surfaces that come in contact with the sample should be solvent rinsed with methylene chloride.
4.3 Avoid cross-contamination between samples by rinsing any materials used for multiple samples
between samples. Do not touch pipettes, graduated cylinders or squirt bottles to any of the glassware.
4.4 Matrix interference.
4.4.1 IS Interference - Low or high internal standard areas may be corrected by diluting the sample or by using the external standard calibration technique if target compounds are not affected by the interference.
4.4.2 Carryover - Contamination may occur from a previous injection. If this is suspected, re-
analyze the sample with instrument blanks before and after the sample. Dirty extracts may require dilution and/or additional clean up to prevent interference, carryover, or overloading the column and detectors. Document any dilution or cleanup procedure in the Instrument Log Book. Footnote the report as appropriate.
4.4.3 Dirty Injection Port - Maintenance such as replacing the injection port liner, seal, and
clipping the front of the column should be done routinely. 4.4.4 Column bleed - Rising baselines late in the chromatogram indicate column bleed. Utilize
the column conditioning techniques outlined by the column manufacturer; trim both ends of the column, or solvent rinse the column.
4.4.5 Chemical Ionization source requires more cleaning than the electron impact source, and
cannot tolerate any residual water in the extracts or any air leaks. If either air or water are introduced into the chemical ionization source, response and mass signal to noise are seriously degraded.
4.4.6 Chemical Ionization can also experience matrix enhancements that have been observed in
matrix fortified spikes (Matrix Spikes). The addition of matrix modifiers such as peanut oil can minimize this affect. This method adds peanut oil to all standards and sample extracts thru the internal standard addition. The peanut oil concentration of the standards and sample extracts is at 0.1 percent.
5. Safety and Precautions
5.1 This method is used to analyze potentially hazardous samples. Prior to performing this analysis, review the MSDS for all standards and reagents to be used. Observe the recommended safety precautions. Protective clothing and safety glasses must be worn when handling samples or reagents, and all manipulations must be performed in a hood.
5.3 Maintain a clean and uncluttered workspace. Return all chemicals, reagents and resultant wastes
to their designated storage area at the completion of the test.
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6. Equipment and Supplies 6.1 2 ml ALS vials, inserts, and crimp top seals. 6.2 Crimper, 12mm 6.3 Analytical column: 30 meter x 0.25 mm I.D. , 0.25 µm df, capillary fused silica . 6.4 Vials - 8 mL, 4 mL and 2 mL screw-cap vials with teflon faced septum, clear and amber. 6.5 Syringes, Class A, gas-tight, or precision bore: 10 µl, 25 µl, 100 µl, 500 µl, 1000 µl. Autosampler
syringes: 10 µl. 6.6 Volumetric flasks (Class A): 5.0 mL, 10.0 mL, 100.0 mL. 6.7 Data System: Agilent MS ChemStation (E.02.00.493) with ThruPut Target Data Analysis Software,
Revision 4.12. 6.8 GCMS system: Agilent 7890A-GC with Agilent 5975 inert XL/EI/CI-MSD equipped with Agilent
7683B autosampler. 6.9 Misc. Chromatography supplies: ferrules- 0.4mm (85%vespel-15% graphite, and 100% graphite)
Restek 4.0mm double gooseneck deactivated injection port liners with glass wool, 1.2 mm inlet seals, 10 mm diameter septa.
6.10 Balance -Mettler analytical balance capable of reading to 0.01g.
7. Reagents and Standards
7.1. Methylene chloride, Burdick & Jackson, High purity solvent. 7.2. Acetone, Mallinkrodt, Nanograde. 7.3. Hexane, Omnisolve, high priority solvent. 7.4. Internal Standards:
7.4.1. 2000 µg/ml, 4,4’-Dibromooctafluorobiphenyl; Accustandard M-625-06-10x 7.4.2. 2000µg/ml PCB-205; Accustandard C-205S
Six-month expiration date upon opening ampule. 7.4.3. Working Internal Standard Solution, 0.02 µg/mL, mix of above standards.
Six month expiration date.
7.5. Calibration Standards (Primary): custom Pyrethroid standard, 1000µg/ml, Accustandard S-12458-R4 Six month expiration date upon opening ampules.
7.6. Surrogate mix: Decachlorobiphenyl, Accustandard, neat, C-209N
One year expiration date upon opening ampule.
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7.7. Working Standards with Surrogate-0.25 ppm, Six month expiration date
7.8. Second source Standards: Custom Pyrethroid standard, 1000µg/ml. Accustandard, S-12458-R4
Prepared independently using a different lot and gravimetric weighing from the primary calibration standard
Six month expiration date upon opening ampule.
8. Sample Preservation and Storage
8.1. The containers used for sampling and storage should be glass or Teflon and have screw caps with Teflon. All samples and extracts should be stored at 4°C.
8.2. Aqueous Samples should be extracted within 3 days, but no later than 14days, of sampling, (Cyhalothrin
and Permethrin potentially lacks stability after 3 days). 8.3. Soils can be frozen to extend the stability time.
9. Quality Control Procedures
9.1. A method blank (MB) is included for every 24-hour extraction batch, or not to exceed 20 environmental samples, and should be subjected to the same procedures as the samples. The blank is spiked with the same surrogate used in the samples. The blank should be free of contamination. If there are any target hits found in the analysis of the blank, a fresh extract aliquot should be prepped and run to determine if the contamination is a result of extraction or instrument contamination. If instrument contamination is suspected, run a solvent blank to confirm the problem.
9.2. At least one LCS must be prepared per analytical batch. Acceptance Criteria for Spiking Compounds in
LCS can be found in Caltest internal QC. Recovery outside of these limits must be checked and noted. Consult Caltest Internal QC for corrective measures.
9.3. A set of Matrix Spike and Matrix Spike Duplicate should be prepared and analyzed per analytical batch.
Acceptance Criteria for Spiking Compounds in MS/MSD can be found in Caltest internal QC. Recovery outside of these limits must be checked and noted. Consult Caltest Internal QC for corrective measures.
9.4. Internal Standard QC, shall be added to every sample in an analytical batch (including QC samples) at
the same level of concentration as in the calibration standards. This is a good point to also add the 0.1% peanut oil to each sample. An area range of plus 100% and minus 50% is calculated from the average ISTD area from the calibration curve or from the continuing calibration standard. An ISTD area outside of the acceptance range necessitates prepping and analyzing a fresh extract to verify the results. If the problem is due to matrix interference, dilute the sample, inject ISTD and analyze. Repeated ISTD failures within the sequence indicate a problem with the system or ISTD solution. Correct any problems and rerun with a fresh ISTD solution. Re-prep fresh sample extracts if new ISTD solution has been prepared.
9.5. Surrogate QC-The surrogates spike level, and the acceptance criteria are listed in Caltest internal QC. 9.6. Qualitative Data Analysis
9.6.1. The qualitative identification of compounds determined by this method is based on retention time,
and on comparison of the sample quantitation, secondary and tertiary ion ratio. The reference selective mass ion ratios must be generated by the laboratory using the conditions of this method. Compounds should be identified as present when the criteria below are met
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9.6.2. The intensities of the characteristic ions of a compound maximize at the same scan or within one scan of each other. Selection of a peak by a data system where identification is based on the presence of chromatographic peaks containing ions specific for the target compound at a known retention time will be accepted as meeting this criterion
9.6.3. The Relative Retention Time of the sample component is within + 0.06 RRT units of the RRT of
the standard component RRT = RT sample Rt Istd
RT sample = Retention Time of sample component RT Istd = Retention Time of internal standard.
9.6.4. The relative intensities of the characteristic ions agree within 30% of the relative intensities of
these ions in the reference selective mass ion ratios. 9.6.5. Each chromatographic peak is quantified independently then the sum of isomers are reported as
a total pesticide residue for each target analyte. 9.6.6. Identification is hampered when sample components (i.e. matrix interferences) are not resolved
chromatographically and produce mass spectra containing ions contributed by more than one analyte. When a peak obviously represents more than one sample component, appropriate selection of analyte spectra and any necessary background spectra subtraction is important. When analytes coelute, the identification criteria can be met, providing unique ions are present, but each analyte spectrum will contain a portion of the individual compounds coeluting.
10. Calibration and Standardization
10.1 Calibration Criteria: The GCMS system must pass the following criteria prior to the analysis of any
samples. Should any criterion not be met, the problem must be corrected before proceeding. (ie: instrument maintenance performed and/or standards and samples rerun.)
10.2 The GCMS system must be tuned prior to sample analysis. Launch Chemstation MSD application
and select Tune function: 10.2.1 Adjust methane reagent gas flow in PCI mode before doing an autotune. While doing the
methane flow in the PCI mode also look for protonated water (amu = 19) this is a good indicator if there is an air leak (water vapor in air);
Note: When an air leak is determined, find and seal the leak, vent the system; then re-
clean the source followed by pump-down of at least 4 hours (consult manufacturer procedures); and initiate another tune in PCI mode again.
10.2.2 Perform the autotune in the PCI mode first. Review the PCI autotune report for air leaks
and proper methane flow. 10.2.3 Then load the NCI tune method and do a second autotune in the NCI mode. If the
autotune completes, it passes. 10.2.4 There is no tune check standard (i.e. DFTPP).
10.3 Initial Calibration -An initial calibration is performed prior to the analysis of any samples using a
minimum of five points containing all the compounds of interest. The laboratory analyses standards that range from 0.5ppb to 500ppb, on-column, representing 0.5 – 500 ng/L report values.
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10.4 Using 4µl injections for the split mode or 3ul injections if only the MSD is being used. Each standard is acquired on the appropriate detector. Tabulate area responses against concentration for each compound of interest including the Internal Standard, and calculate the Response Factor (RF) for each compound using the following equation: RF = (AsCis)/(AisCs) where:
As = Response for the parameter to be measured. Ais = Response for the Internal Standard. Cis = Concentration of the Internal Standard (µg/mL). Cs = Concentration of the parameter to be measured (µg/mL).
The results are used to plot a calibration curve of Response Ratios, vs. RF. The linearity of the curve for each compound is checked and adjustments are made as necessary. A calibration curve is considered linear (acceptable) if the average RF is <= 15% and/or the grand mean average RF of all the target analytes is <=15%. If a quadratic curve is used you must use no less than 6 calibration points and achieve a correlation coefficient of 0.995 or higher (approaching 1.0).
10.4 A mid-level secondary standard is injected following the calibration curve to verify the validity of the primary standards. This standard is from a different Lot # and gravimetric weighing than those purchased for the initial calibration. All compounds should meet 30% acceptance criteria. Should any compound not meet the criteria, the samples will be additionally reviewed for that compound. Should the sample prove positive for that compound, the discrepancy between the calibration and the secondary standard will be resolved and the sample re run.
11. Procedure
11.1 Proper documentation is essential at all points of sample preparation. 11.1.1 Before preparing any sample extract for analysis, check the extraction sheet for any
comments concerning extraction and multiplier, check the Semi-volatiles Worklist for any work notes, test requested, age, client ID, matrix, and sample description. Confirm any anomalies with the Confirmation order or the Chain of Custody. Notify the department manager or project manager if there are any discrepancies.
11.1.2 The GCMS Instrument Run Log must be created during the course of the analysis.
See SOP Q-LOGBOOK for the correct procedure for logbook entries. The following information is needed to complete the GCMS Instrument Logbook (See Appendix 1 for a sample of the logbook page).
11.1.2.1 Operator - Enter the Initials of the analyst(s) responsible for loading the
samples and standards. 11.1.2.2 Method - Enter acquisition and data analysis method if separate. 11.1.2.3 Column - Analytical column 11.1.2.4 Comments-Lot #s for Standards, detailed information about sample dilutions (ie:
100µl-500µl=5x), and any other comments. 11.1.3 All containers or glassware used to hold the samples or QC should be clearly marked
to prevent confusions or switching of samples.
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11.1.4 ALS vials-Label with sample number, date extract was prepared for analysis, Det code, and any dilution made to the sample.
11.1.5 The Semivolatiles Standards Log Book must be completed whenever standards or
reagents are prepared. See SOP Q-LOGBOOK for the correct procedure for logbook entries.
11.1.6 MSDF Maintenance Log Book: Any maintenance; routine or special, column changes,
additions or changes performed on the system must be recorded in the MSDF Maintenance Log Book. The result of any maintenance procedures must also be recorded.
11.2 Acquisition
11.2.1 Refer to instrument maintenance logbook for run instrument parameters. Update run
instrument parameters every time they are changed.
11.3 Analysis
11.3.1 The samples are extracted according to Caltest SOP’s O-3510PREP, O-3550PREP and O-GCMSPREP. ALS vials are labeled appropriately. Samples may be diluted with hexane. The ISTD must have equilibrated at room temperature before use. A dilution on the sample, or a new curve must be prepared that will properly bracket the concentration of that compound. All dilutions are recorded on the extract vial, in the Instrument Log Book, within the analytical sequence run log and entered into Target Analytical Software for each sample.
11.3.2 Sequence - A typical sequence is:
11.3.2.1 Priming standards (up to five injections @ 500ppb) 11.3.2.2 ICAL 0.5 to 500ppb (5 to 12 levels) 11.3.2.3 Secondary source standard, mid level 11.3.2.4 Response consistency check standards (up to five injections @ 20ppb) 11.3.2.5 Bracketing standards @ 5,20 and 50ppb, alternating 1-2 levels bracketing up to
four injections until closing response check sample 11.3.2.6 QC samples-MB, LCS, LCSD 11.3.2.7 Samples, MS, MSD. 11.3.2.8 Closing response check (0.5ppb) to check system sensitivity check.
11.4 Sample extracts in the refrigerator at ≤ 6°C until prepped and analyzed. Analyzed extracts should be archived until the data is reviewed for completeness. Neat extracts will be kept for 60 days past the extraction date. All extracts must be disposed in the proper manner.
11.5 Data Reporting, and Archival
11.5.1 Generate Quant Reports using Target software for samples and QC. IS and surrogate recoveries must pass QC criteria outlined in section 9 of this document. Corrective steps
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may be taken when necessary to achieve QC requirements (ie: manual integration of a peak, dilution of the sample, or other measures
11.5.2 Using the Review feature of the Target software, check any hits that are suspect. Manual
integration of a peak may be necessary to accurately quantitate the sample. Regenerate the report after any changes are made.
11.5.3 Taking into account the multiplier for the sample, report concentrations of compounds that
meet all of the following criteria:
11.5.3.1 The mass spectral data meets the criteria set forth in section 10. 11.5.3.2 The peak has a retention time that falls within the retention time window. See
section 10. 11.5.3.3 The concentration calculated is above the reporting limit taking into account the
sample extraction and dilution factors. 11.5.3.4 The experience of the analyst must weigh heavily in the interpretation of
chromatograms.
11.6 Sample Data Entry - When reporting sample results on the LIMS system, enter the following data
as appropriate:
11.6.1 DILUTION FACTOR: The detection limits in LIMS assume a dilution factor of 1 reflecting a 1000mL -> 1mL prep for aqueous samples and a 15g -> 2mL for soils/sludges. All dilutions entered in LIMS should be rounded to one significant figure). To calculate dilution factor multipliers, when two or more dilutions were made on a sample, enter the product dilution factor.
11.6.2 RESULTS – Post results in LIMS as raw data values, LIMS will report results in proper
significant figures or “ND” for non-detected target compounds. 11.7 To report QC results on LIMS, first generate a TARGET QC report. All QC data results posted to
LIMS using raw data with a minimum of three significant figures. 11.8 Completed data results are to be submitted for second-party review to a designated competent,
trained analyst reviewer familiar with this analysis or the Technical Lead/Coordinator/Lab Director for data approval and filing.
11.9 Trouble shooting
11.9.1 The most common causes of loss of response are a dirty source and / or an air leak. The GC inlet septum should be replaced frequently due to multiple punctures of a septum are a source of air leakage when a detector is under vacuum such as a mass spectrometer. Other low response checks include the auto sampler syringe for breakage or partial blockage. Check ferrule nuts at injector and detector and tighten if necessary. Check gas supplies and check for leaks. Check for septum particles in the injector liner. Cut several inches of column from the injector end and/or detector end. Low responses may also be caused poorly performing filaments, or an old multiplier (voltage >2400mV)
11.9.2 No peaks-Check the autosampler syringe for breakage and replace if necessary. Check if
analytical column is broken or leaking. Check the gas supplies and replace tanks as necessary. Verify flow through the column. Check injector liner for septum debris.
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11.9.4 Large, misshapen peaks-Compounds in the sample may be overloading the detector.
Dilute the sample and rerun. 11.9.5 Noisy baseline- remove column nuts and check for crushed ferrule and/or column.
Remove a few inches of column, replace with a new ferrule and bake out system at 325° C for 30 minutes and check for baseline stability. Further problems may indicate a contaminated column requiring solvent rinsing or replacement.
12. Calculations
12.1 The internal standard technique -The Enviroquant GC software and Target Analytical software automatically calculates the concentrations of analytes using the following equation:
Concentration, µg/L = (As) (Is) (Ais) (RF)
As = Quant ion response for the compound to be measured. Is = Amount of internal standard. Ais= Quant ion response for the internal standard. RF = Response factor
12.2 The external standard technique is to be employed as in cases where there is interference with
the IS or detector drift or matrix enhancements. The concentration can be determined by using the following equation:
Concentration µg/L = Auk x Cs As Auk = Quant ion response of the compound being measured. As = Quant ion response of the compound in the standard. Cs = Concentration of the standard.
12.3 Multipliers are used to relate the results calculated in the final analysis to the reported units.
12.3.1 Liquid matrices Analysis units = ng/mL-ppb Reporting units = ng/L-ppt Result: on column amount x Final Volume x Dilution Factor Initial Volume
12.3.2 Solid matrices Analysis units = ng/mL-ppb Reporting units = µg/Kg-ppb
Result: on column amount x Final Volume x Dilution Factor Initial Volume
13. Pollution Prevention
13.1 There are no particular pollution prevention steps taken by the laboratory for this test.
14. Data Assessment and Acceptance Criteria for Quality Control Measures.
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14.1 The data is referenced in Caltest’s internal QC summary manual.
15. Corrective Actions for Out of Control Data. 15.1 The data is referenced in Caltest’s internal QC summary manual. 16. Contingencies for Handling Out-Of-Control or Unacceptable Data.
16.1 The contingencies for handling out-of-control or unacceptable data are addressed in the Caltest
SOP: Q-CORRECT. 17. Waste Management
17.1 All solvent saturated aqueous waste is collected in a drum. The waste is shipped out for disposal using a hazard waste disposal company. It is profiled as waste corrosive liquid.
17.2 All dichloromethane is collected in a solvent drum. The waste is shipped out for disposal using a
hazard waste disposal company. It is profiled as waste Dichloromethane. 17.3 All Acetone waste is collected in a drum. The waste is shipped out for disposal using a hazard
waste disposal company. It is profiled as waste flammable liquid. 17.4 All waste handling in performed in accordance with Caltest waste handling procedures.
18. Method Performance
18.1 Certified Reference and Performance Evaluation materials are not currently available specific to Pyrethroids analysis.
19. References
19.1 Analytical Method For The Determination Of Synthetic Pyrethroids in Sediment by Gas Chromatography-Negative Chemical Ionization Mass Spectometry. Neil J. Robinson, Syngenta Ltd. (2006).
19.2 EPA SW-846 8270 Semivolatile Organic Compounds By Gas Chromatography/Mass Spectrometry (GC/MS)
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Appendix 1
Pyrethroid List of Analytes
Pyrethroid
Analyte CAS Number
Ret Time
1º 2º 3º
Bifenthrin (Biphenthrin) 82657-04-3 19.7 386 387 241
Cyfluthrin (Baythroid) 68359-37-5 24.4 207 209 171
Lambda-Cyhalothrin 91465-08-6 21.5 241 205 243
Cypermethrin 52315-07-8 24.9 207 209 171 Fenvalerate / Esfenvalerate
(Pydrin) 51630-58-1
26.7 211 213 212
Tau-Fluvalinate 102851-06-9 26.7 294 296 295 Fenpropathrin
*(Danitol) 39515-41-8 20.0 141
Permethrin 52645-53-1 23.3 207 209 Tralomethrin/ Deltamethrin 66841-25-6 27.8 81 295 297
Allethrin 584-79-2 13.6 167 168 Tetramethrin 7696-12-0 19.8 331 332
Additional Analyte per request:
Analyte CAS
Number 1º 2º 3º
Chlorpyrifos 2921-88-2 12.3 315 214 313 *Diazinon 333-41-5 9.6 169
Ret Time = retention time.
1º = Quantitation Ion 2º = Qualifying Ion 3º = Monitoring Ion
* = NCI only produces one ion, the compound qualifies using 1º and retention time.
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Adminsitrative Record Page 19361
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Adminsitrative Record Page 19362
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Adminsitrative Record Page 19370
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Adminsitrative Record Page 19371
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Adminsitrative Record Page 19372
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Adminsitrative Record Page 19373
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Adminsitrative Record Page 19374
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Adminsitrative Record Page 19375
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Adminsitrative Record Page 19376
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Adminsitrative Record Page 19377
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Adminsitrative Record Page 19378
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Adminsitrative Record Page 19379
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Adminsitrative Record Page 19380
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Adminsitrative Record Page 19381
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Adminsitrative Record Page 19382
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Adminsitrative Record Page 19383
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Adminsitrative Record Page 19384
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Adminsitrative Record Page 19385
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Adminsitrative Record Page 19386
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Adminsitrative Record Page 19387
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Adminsitrative Record Page 19388
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Adminsitrative Record Page 19389
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Adminsitrative Record Page 19390
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Adminsitrative Record Page 19391
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Adminsitrative Record Page 19392
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Adminsitrative Record Page 19393
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Adminsitrative Record Page 19394
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Adminsitrative Record Page 19395
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Adminsitrative Record Page 19396
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Adminsitrative Record Page 19397
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Adminsitrative Record Page 19398
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Adminsitrative Record Page 19399
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Adminsitrative Record Page 19400
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Adminsitrative Record Page 19401
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Adminsitrative Record Page 19402
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Adminsitrative Record Page 19403
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Adminsitrative Record Page 19404
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Adminsitrative Record Page 19405
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Adminsitrative Record Page 19406
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Adminsitrative Record Page 19407
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Adminsitrative Record Page 19408
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Adminsitrative Record Page 19409
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Adminsitrative Record Page 19410
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Adminsitrative Record Page 19411
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Adminsitrative Record Page 19412
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Adminsitrative Record Page 19413
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Adminsitrative Record Page 19414
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Adminsitrative Record Page 19415
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Adminsitrative Record Page 19416
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Adminsitrative Record Page 19417
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Adminsitrative Record Page 19418
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Adminsitrative Record Page 19419
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Adminsitrative Record Page 19420
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Adminsitrative Record Page 19421
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Adminsitrative Record Page 19422
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Adminsitrative Record Page 19423
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Adminsitrative Record Page 19424
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Adminsitrative Record Page 19425
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Adminsitrative Record Page 19426
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Adminsitrative Record Page 19427
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Adminsitrative Record Page 19428
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Adminsitrative Record Page 19429
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Adminsitrative Record Page 19430
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Adminsitrative Record Page 19431
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Adminsitrative Record Page 19432
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Adminsitrative Record Page 19433
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Adminsitrative Record Page 19434
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Adminsitrative Record Page 19435
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Adminsitrative Record Page 19436
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Adminsitrative Record Page 19437
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Adminsitrative Record Page 19438
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Adminsitrative Record Page 19448
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Adminsitrative Record Page 19449
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Adminsitrative Record Page 19451
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Adminsitrative Record Page 19452
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Adminsitrative Record Page 19453
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Adminsitrative Record Page 19454
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Adminsitrative Record Page 19456
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Adminsitrative Record Page 19458
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Adminsitrative Record Page 19459
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Adminsitrative Record Page 19461
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Adminsitrative Record Page 19462
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Adminsitrative Record Page 19463
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Adminsitrative Record Page 19471
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Adminsitrative Record Page 19472
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Adminsitrative Record Page 19473
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Adminsitrative Record Page 19474
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Adminsitrative Record Page 19476
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Adminsitrative Record Page 19478
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Adminsitrative Record Page 19479
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Adminsitrative Record Page 19481
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Adminsitrative Record Page 19482
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Adminsitrative Record Page 19483
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Adminsitrative Record Page 19484
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Adminsitrative Record Page 19486
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Adminsitrative Record Page 19487
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Adminsitrative Record Page 19489
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Adminsitrative Record Page 19491
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Adminsitrative Record Page 19492
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Adminsitrative Record Page 19493
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Adminsitrative Record Page 19494
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Adminsitrative Record Page 19496
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Adminsitrative Record Page 19498
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Adminsitrative Record Page 19502
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Adminsitrative Record Page 19503
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Adminsitrative Record Page 19504
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Adminsitrative Record Page 19506
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Adminsitrative Record Page 19507
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Adminsitrative Record Page 19508
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Adminsitrative Record Page 19509
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Adminsitrative Record Page 19551
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Adminsitrative Record Page 19552
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Adminsitrative Record Page 19553
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Adminsitrative Record Page 19554
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Adminsitrative Record Page 19555
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Adminsitrative Record Page 19556
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Adminsitrative Record Page 19557
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Adminsitrative Record Page 19558
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Adminsitrative Record Page 19559
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Adminsitrative Record Page 19561
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Adminsitrative Record Page 19562
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Adminsitrative Record Page 19563
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Adminsitrative Record Page 19565
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Adminsitrative Record Page 19571
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Adminsitrative Record Page 19572
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Adminsitrative Record Page 19573
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Adminsitrative Record Page 19574
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Adminsitrative Record Page 19576
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Adminsitrative Record Page 19578
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Adminsitrative Record Page 19591
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Adminsitrative Record Page 19592
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Adminsitrative Record Page 19593
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Adminsitrative Record Page 19594
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Adminsitrative Record Page 19596
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Adminsitrative Record Page 19597
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Adminsitrative Record Page 19598
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Adminsitrative Record Page 19599
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Adminsitrative Record Page 19602
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Adminsitrative Record Page 19603
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Adminsitrative Record Page 19604
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Adminsitrative Record Page 19605
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Adminsitrative Record Page 19606
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Adminsitrative Record Page 19607
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Adminsitrative Record Page 19608
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Adminsitrative Record Page 19609
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Adminsitrative Record Page 19610
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Adminsitrative Record Page 19635
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Adminsitrative Record Page 19636
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Adminsitrative Record Page 19637
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Adminsitrative Record Page 19638
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Adminsitrative Record Page 19639
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Adminsitrative Record Page 19640
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Adminsitrative Record Page 19641
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Adminsitrative Record Page 19642
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Adminsitrative Record Page 19643
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Adminsitrative Record Page 19644
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Adminsitrative Record Page 19645
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Adminsitrative Record Page 19646
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Adminsitrative Record Page 19647
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Adminsitrative Record Page 19648
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Adminsitrative Record Page 19649
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Adminsitrative Record Page 19650
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Adminsitrative Record Page 19651
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Adminsitrative Record Page 19652
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Adminsitrative Record Page 19653
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Adminsitrative Record Page 19654
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Adminsitrative Record Page 19655
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Adminsitrative Record Page 19656
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Adminsitrative Record Page 19657
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Adminsitrative Record Page 19658
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Adminsitrative Record Page 19659
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Adminsitrative Record Page 19660
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Adminsitrative Record Page 19661
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Adminsitrative Record Page 19662
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Adminsitrative Record Page 19663
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Adminsitrative Record Page 19664
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Adminsitrative Record Page 19665
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Adminsitrative Record Page 19666
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Adminsitrative Record Page 19667
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Adminsitrative Record Page 19668
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Adminsitrative Record Page 19671
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Adminsitrative Record Page 19672
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Adminsitrative Record Page 19673
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Adminsitrative Record Page 19674
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Adminsitrative Record Page 19675
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Adminsitrative Record Page 19676
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Adminsitrative Record Page 19677
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Adminsitrative Record Page 19678
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Adminsitrative Record Page 19679
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Adminsitrative Record Page 19680
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Adminsitrative Record Page 19681
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Adminsitrative Record Page 19682
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Adminsitrative Record Page 19683
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Adminsitrative Record Page 19684
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Adminsitrative Record Page 19685
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Adminsitrative Record Page 19686
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Adminsitrative Record Page 19687
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Adminsitrative Record Page 19688
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Adminsitrative Record Page 19689
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Adminsitrative Record Page 19690
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Adminsitrative Record Page 19691
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Adminsitrative Record Page 19692
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Adminsitrative Record Page 19693
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Adminsitrative Record Page 19694
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Adminsitrative Record Page 19695
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Adminsitrative Record Page 19696
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Adminsitrative Record Page 19697
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Adminsitrative Record Page 19698
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Adminsitrative Record Page 19699
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Adminsitrative Record Page 19700
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Adminsitrative Record Page 19701
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Adminsitrative Record Page 19702
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Adminsitrative Record Page 19703
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Adminsitrative Record Page 19704
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Adminsitrative Record Page 19705
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Adminsitrative Record Page 19706
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Adminsitrative Record Page 19707
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Adminsitrative Record Page 19708
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Adminsitrative Record Page 19709
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Adminsitrative Record Page 19710
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Adminsitrative Record Page 19711
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Adminsitrative Record Page 19712
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Adminsitrative Record Page 19713
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Adminsitrative Record Page 19714
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Adminsitrative Record Page 19715
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Adminsitrative Record Page 19716
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Adminsitrative Record Page 19717
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Adminsitrative Record Page 19718
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Adminsitrative Record Page 19719
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Adminsitrative Record Page 19720
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Adminsitrative Record Page 19721
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Adminsitrative Record Page 19722
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Adminsitrative Record Page 19723
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Adminsitrative Record Page 19724
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Adminsitrative Record Page 19725
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Adminsitrative Record Page 19726
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Adminsitrative Record Page 19727
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Adminsitrative Record Page 19728
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Adminsitrative Record Page 19729
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Adminsitrative Record Page 19730
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Adminsitrative Record Page 19731
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Adminsitrative Record Page 19732
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Adminsitrative Record Page 19733
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Adminsitrative Record Page 19734
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Adminsitrative Record Page 19735
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Adminsitrative Record Page 19736