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Acidfaststaining 090911062148 Phpapp02
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Transcript of Acidfaststaining 090911062148 Phpapp02
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ACID FAST STAINING
SUMMARY Microbiology Lab 11th Sept
Ravi A Patel
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2 PROCEDURES FOR ACID FAST STAINING
Ziehl Neelsen’s Method (Hot Method)
------The one presented as Demo
Kinyoun’s Method (Cold Method)
------The one which we performed today
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SPECIAL STRUCTURES WE SAW
Flagella
Capsule
Metachromatic granules
Spores
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SMEAR ON THE SLIDE MADE FROM SPUTUM OF THE PATIENT HAVING TB
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ZIEHL NEELSEN’S METHOD-HOT METHOD
+VE & -VE ACID FAST STAINING------? What does it mean
------If the Organism does not take the counter stain color(In this case Methylene blue) and sustains its primary stain color(Red) then it’s a +ve acid fast staining
And Vice Versa…………..for –ve Acid Fast staining
* Involves heating for 5 min on slow burner after being stained with primary stain so called Hot Method unlike the Kinyoun’s in which we did not use burner after primary staining…..
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ZIEHL NEELSEN’S METHOD
In the slide we saw the Red colored +ve Acid fast stained Bacilli although the blue colored background was not so prominent..
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KINYOUN’S METHOD Group-9 got a –ve acid fast staining bacilli Group-2,3,7 got +ve Acid fast staining Bacilli This picture is of +ve Acid fast staining bacilli If we look
closely at the pointer there is a red bacilliover greenbackground of malachitegreen
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SPECIAL STRUCTURES
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FLAGELLA
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FLAGELLA Stained by :- Liefson’s stain
Function :- Locomotion
Composition :- Protein Flagellin
Types :- Mono-Amphi-Lopho-Peri-Atrichous
( Acronymn- P-A-A-L-M )
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CAPSULEStructure at the pointer capsule and within capsule we see mycobacterium
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CAPSULE
Stained by :- Hiss stain
Function :- Antiphagocytosis Protects the bacteria(Virulence of the bacteria)
Charateristic:- Antigenic
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STRUCT. AT THE POINTERMETACHROMATIC GRANULES
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METACHROMATIC GRANULES
Stained by :- L.A.M.B stain(loeffler’s Alkaline methylene blue)
Function:- Temporarily stores metabolites(carbon, phosphates)
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STUCTURE AT THE POINTER SPORES
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SPORES(ENDOSPORES)
Stained by:- Dorner stain
Function:- Provides resistance to bacteria in adverse conditions
Characteristic:- Spores are dormant cellsThey can undergo
germination
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THANKING TO THE ENTIRE UNIVERSE