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Transcript of Abstract Book - Kumaun · PDF fileAbstract Book National Seminar ... The Botany Department,...
1
Abstract Book
National Seminar
on
“Plants and Environment
(NSSAPPE-2017)”
March 27 & 28, 2017
Organized
by
Department of Botany, D. S. B. Campus,
Under SAP, UGC Programme
Kumaun University, Nainital
2
3
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About the University
Kumaun University was established in 1973. It consists of three campuses at Almora,
Nainital and Bhimtal. More than 100 affiliated government colleges and self-financed private
institutions spread across the Kumaun region. In the affiliated campuses, both teaching and
research activities are equally important factors that support the curriculum and contribute to
the student's learning habits. NAAC accredited ‘A’ grade to Kumaun University in 2016.
About the Botany Department
The Botany department was established in 1951 as a government postgraduate college and
since 1973, it is functioning as a department of University. The department has been graced
by many nationally and internationally known scientists/ teachers including Prof. K.S.
Bhargava, Prof. S.C. Gupta, Prof. B.S. Mehrotra, Prof. J. S. Singh, Prof. S.P. Singh, Prof.
R.D. Khulbe, Prof. Y.P.S. Pangtey, Prof. Gira Bala Pant, Prof. Sudhir Chandra and Prof. Uma
Palni. Prof. J .S. Singh was awarded FNA and Shanti Swaroop Bhatnagar prize. Prof. S.P.
Singh was awarded FNA and Birbal Sahni medal and also worked as Hon’ble Vice
Chancellor, Garhwal University, Srinagar. Prof. Y.P.S. Pangtey, NASI fellow was awarded
FNASc and worked as UGC, Emeritus Fellow. The department has been included under FIST
programme, DST, New Delhi and recognized as DRS (SAP) department, UGC New Delhi.
The department actively collaborated with major institutions like G.B. Pant National Institute
of Himalayan Environment and Sustainable Development, Kosi, Katarmal- Almora, NBRI
Lucknow, NBPGR Bhowali, CIMAP, Lucknow, BSI, Dehradun, RRIHF, CCRAS, Tarikhet
and CAP, Selaqua. A large number of research projects and several collaborative research
programmes involving various national and international agencies have been completed and
ongoing. Over 500 research papers have been published in more than 50 journals of national
and international repute and about 250 students have been awarded Doctoral degree in past
two decades. Specimens of Angiosperms (2500), Pteridophytes (300), Bryophytes (>200),
Fungi (>100) and Lichens (nearly 100) have been preserved in herbarium. Presently semester
courses at undergraduate, postgraduate and course system Ph. D. are being run in the
department. At Post Graduate level, specializations are running in various disciplines viz.,
Forest Ecology, Bryology, Plant Pathology, Ethonobotany and Plant Taxonomy. The
department is regularly publishing an annual news letter “OAKS” for last ten years. The
department has well equipped and computerized laboratories of Plant Tissue Culture, Plant
Pathology and Aquatic Mycology, Ecology, Taxonomy and Bryology. At present, the
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department has two glass houses, one mist chamber to carry out various green house
experiments and research activities. The major equipments available in the department for
research activities are Spectrophotometer, Pressure chamber, Laminar air flow chamber, ultra
cool centrifuge, lypholizer, BOD Incubator, seed germinator, LCDs, Mico Kjeldhal unit,
Microscopes, Xerox machines, Research Microscopes, Multi media facility, Fax facility etc.
About the National Conference
The Botany Department, D.S.B. Campus Kumaun University, Nainital, Uttarakhand, India is
organising a National Conference on 27 & 28 March 7 The plenary, key notes and invited
talks by eminent Scientist of this region would give insight to the current research scenario
for the benefits to the society.
The seminar will cover the following areas:
Systematic Biology
Drug Design
Natural Product
Ecosystem
Environment
Biotechnology
Microbiology
Botany Department of Nainital was established on 1951 and reorganised in 1973 with the
establishment of the Kumaun University, Nainital. The world class research centre of
Uttarakhand working in the several of Plant Sciences. This conference is organised under the
SAP- UGC programme of the Department.
6
Patron
Hon’ble Vice Chancellor, Kumaun University, Nainital
Chairman
Prof. S.P.S. Mehta, Director D. S. B. Campus, Nainital
Co-Chairman
Prof Neerja Pande, Head, Department of Botany, D.S.B. Campus, Nainital
Convener
Prof. S. C. Sati, Department of Botany, D.S.B. Campus, Nainital
Co-convener
Prof. Y. S. Rawat, Department of Botany, D. S. B. Campus, Nainital
Organising Secretary
Prof. Lalit Tewari, Department of Botany, D. S. B. Campus, Nainital
Joint Secretary
Dr. Kiran Bargali, Dr. Sushma Tamta Dr. Neelu Lodhiyal, Dr. Anil Bisht, Dr. Kapil Khulbe,
D. S. B. Campus, Nainital
Advisory Board
Prof. Santosh Kumar, Dean Science, Prof. P. C. Pande, Prof. Hema Joshi, Prof. S. S. Gahlan
Prof. S. S. Bargali
Members
Dr. Yogesh Joshi, Dr. Balwant Kumar, Dr. Dhani Arya, Dr. Ashish Tewari, Dr. Veena Pande,
Sri Yugal Joshi, Sri Suchetan Sah, Dr. Geeta Teawri, Dr. Tapan Nailwal, Dr. Santosh
Upadhyay, Dr. Penny Joshi, Dr. Himanshu Lohani, Dr. Sohail Javed, Dr. Vijay Kumar, Dr.
Lalit Mohan
National Advisory Committee
Prof. D. K. Maheshwari, Haridwar, Prof. A. K. Pandey, New Delhi, Prof L. M. S. Palni,
Dehradun, Prof. Y. P. S. Pangtey, Haldwani, Prof. Sudhir Chandra, Nainital
Prof. Uma Palni, Dehradun, Dr. Gopal Rawat, Dehradun, Dr. S. S. Samant, Mohal, Kulu
Prof. Jeet Ram, Nainital, Prof. L. S. Lodhiyal, Nainital, Dr. Jyoti Saxena, Dwarahat,
Prof. Uma Melkania, Pantnagar, Dr R.S. Rawal, Katarmal, Prof P. B. Rao, Pantnagar,
Dr G. C. Joshi, Tarikhet, Dr. B. S. Kalakoti, Haldwani, Dr. I. S. Bisht, Niglat, Prof. Satish
Garkoti, J. N. U., New Delhi, Dr. D. K. Upreti, N. B. R. I., Lucknow, Prof. R. C. Dubey,
Haridwar, Prof. S. V. S. Chauhan, Agra, Dr. Rajnish Agnihotri, Agra, Prof. N. N. Tripathi,
Gorakhpur, Prof. Chitra Pande, Nainital, Prof. N. K. Dubey, Varanasi, Prof. V. P. Singh,
Delhi, Prof. Arun Arya, Barodara, Dr. Virendra Nath, Lucknow, Prof. N. S. Bisht, Pauri,
Prof. Ganga Bisht, Prof. Suchi Bisht, Prof. Sanjay Pant, Prof. C. K. Pant, Dr. N. G. Sahoo,
Nainital, Dr. P. B. Tewari, Chandausi
Speakers
Prof. Nageshwar Rao, Prof. S. P. S. Mehta, Prof. Santosh Kumar, Prof. Uma Melkania. Dr.
B. S. Kalakoti, Dr Amit Pandey, Dr. I. D. Bhatt, Dr. G. C. S. Negi, Prof. Neerja Pande, Prof.
7
S. C. Sati, Prof. Ganga Bisht, Prof. P. C. Pande, Prof. Lalit Tewari, Dr. Ashish Tewari, Dr.
Yogesh Joshi
Panel of experts
Prof. Sudhir Chandra, Dr. S. D. Tewari, Dr. Amit Pandey
Contacts
Prof. S. C. Sati Prof. Y. S. Rawat Prof. Lalit Tewari
Convener Co –Convenor Organising Secretary
Mob.: 9412969960 Mob.: 9719293980 Mob.: 9412362085
8
Abstracts
9
Index
S.
No.
Name of
Participant
Title Page no.
1 Bharat Giri
Gosain
Flow of Forest Ecosystem Services with Special Emphasis on C-
Sequestration in a Mountain Watershed, Kumaun Himalaya
12
2 Vijay Arya Ecosystem Services in Temperate Sacred Forests of Kumaun Himalaya 13
3 Rajni Rawat, Distribution and Diversity of Ocimum Species Occurring in Different Agro-
Ecologies of Uttarakhand State
14
4 Kamla Dhyani Allelopathic Effect of Leaf Extract of (Poplar Deltoidus) in Seed
Germination, Root Characters and Physiology of Rice Seedlings
15
5 Niraj Singh Development and Application of Scar Markers for Identification of
Gladiolus Germplasm
16
6 Harsh Chauhan Rapid Vulnerability Assessment of Trillium Govanianum Wall. Ex D. Don;
A Commercially Exploited Medicinal Plant of Himalaya
17
7 Indu Tiwari Impact of Environmental Factors on the Growth of Plants 18
8 Prachi Tamta Observation on Status of Insect Pollinators in Binsar Wildlife Sanctuary,
Western Himalayas, Uttarakhand, India
19
9 Mahesha Nand Qsar Based Virtual Screening of Novel Phytochemical Inhibitors Against
Her2 in Breast Cancer
20
10 Preeti Bhatt Biochemical Aspects of Photosynthesis and Respiration: An Implication to
Environment and Social Awareness
21
11 Meenakshi Negi Analysis of Forest Vegetation, Population Structure And Regeneration
Pattern In Kumaun Himalaya
22
12 Hemlata Rathore A Seasonal (Rainy Season) Survey and Study on the Availability of
Different Fodder Species of Kumaun and Garhwal Region of Uttarakhand
23
13 Prabha B.
Dhondiyal
Allelopathic Effect of Bryophytes on Germination and Growth of Bidens
Biternata Lour.) Merr & Shreff
24
14 Netrapal Sharma Genetic Diversity among Sea Buckthorn (Hippophae Rhamnoides (L)) using
Molecular Marker based Analysis
25
15 Balwant Kumar Impact of Lichen Litter on Soil Nutrients in the Temperate Areas of Kumaun
Himalaya (Uttarakhand)
26
16 Mahesh
Chandra Arya
Adsorptive Removal of Zinc (II) by Activated Bark of Pinus Roxburghii :
An Ecofriendly Approach
27
17 Uzma Siddiqui Observation on Environmental Effect on the Breeding Dynamics of
Schizothorax Richardsonii (Gray), from Kumaun Himalayas, Uttarakhand,
India
28
18 Pushpa Bhakuni Antimalarial activities of some Plant Species Found in Kumaun Region
Uttarakhand
29
19 Sapna A Review of Chemical Analyses and Ethno-Medicinal Uses of
Zingiberaceae Family
30
20 Mamta Bisht Effect of Zinc on Growth and Herbage Yield of Ocimum Gratissimum L. 31
21 Bhanu Kumar Chemotypic Variations in Ageratum Conyzoides L. Collected from Indo-
Gangetic Plains 32
22 Akanksha Rani Essential Oil Composition of Murraya Koenigii Collected from Three
Locations of North India
33
23 Brij M. Upreti Physico Chemical Characterization of Soil Collected From Sacred and Non
Sacred Forests of Uttarakhand: A Comparative Study
34
24 Vandana Jaggi, Confirmation of Interacting Partners of Chickpea DNA Methytransferases
Clones and their Interaction With Ago Protein
35
10
25 Deepshekha
Punetha
Heavy Metal Accumulation by Coriandrum Sativum in Cow Dung Amended
Soils
36
26 Sunita Bhatt Oven Dried Volatile Constituents of Ocimum americanum: A Rich Source
of Bioactive Compounds
37
27 Jitendra Bhatt Changes in Biodiversity and Regeneration Pattern in Plant
Communities after Eradication of Lantana Camara L. From the Forest
Areas of Uttarakhand
38
28 Nandan Singh Tree Water Relation and Regeneration Status of Treeline Species in
Tungnath Area of Uttarakhand
39
29 Shruti Shah Assessing Regeneration Potential and Developing Reliable Indicators
of Seed Maturity in Myrica esculenta, Buch-Ham. Ex D. Don, in
Kumaun Central Himalayan Region
40
30 Bhawna Tewari Developing Nursery Techniques for Propagation of Prunus cerasoides
D. Don
41
31 Pritee Pant Antioxidant and Phytochemical Analysis of two Medicinal Plants of
Kumaun Himalaya
42
32 Neeta Arya Diversity Analysis in Pinus Roxburghii Sarg. and Mixed Broadleaf Forest of
Central Hiimalaya, India
43
33 Neha Rajwar Solid Waste Management through Vermicomposting and Earthworm Gut
Cellulolytic Bacteria
44
34 Poonam Prasad A Comparison of Canopy Cover Measured through Transect and
Densiometer Methods in Oak Forest of Central Himalaya India
45
35 Rajesh Kumar Zirconium Doped Iron Oxide Nanocomposite for the Adsorption of Cd (Ii) :
An Eco-Friendly Protocol 46
36 Jeewan Gahtori A comparative study on water quality of river Saryu for drinking purposed
in Bageshwer District
47
37 Anita Rana Density relationship for adsorption capacity of Liriodendron tulipifera (hard
wood) and Cedrus deodara (soft wood)
48
38 Urvashi Verma Quantitative estimation of total Phenolic content, total flavonoid content and
free radical scavenging potential of Himalayan Fir (Abies pindrow (Royle ex
D.Don) Royle) : An Ethnomedicinally Important Plant
49
39 Krishna Kumar
Tamta
Economic Upliftment of Local Communities through Cheura (Diploknema
Butyracea Roxb.) Plantations in Kumaun
50
40 KaminiTamta Biomass Status In Ungrazed And Grazed Oak Forest Grazingland In The
Himalaya, India
51
41 Naveen
Chandra
Pandey
Ethnobotanical Studies of Kosi Catchment Area, Kumaun Himalya 52
42 Sumita Rana Cultivated Exotic Medicinal Plants in Tarai Belt of Uttarakhand, India 53
43 Prabha Pant Screening for Phosphate Solubilization Potential of Two Rhizospheric Fungi 54
44 Parikshit Kumar Phytochemical analysis and evaluation of Kumaun Himalayan Litsea
umbrosa Nees bark extract for its antimicrobial and antioxidant potentiality
55
45 Amit Mittal Impact of Winter Water Stress on Seedlings of Quercus Leucotrichophora
A.Campus and Rhododendron Arboreum Wall
56
46 Kanchan Upreti Ethnobotany and Indigenous Uses of Urtica dioica L. in Uttarakhand
Himalaya 57
47 Geeta Tewari Essential Oil Composition and Antifungal Activity of Pimpinella
Achilleifolia (DC) Clark
58
48 Neelaxi Pandey Nutraceutical Plant Diversity in Kumaun Himalaya of Uttarakhand, India 59
49 Pankaj Kumar Biotechnological Traditional Knowledge and Related IPR Issues 60
50 Pramod Kumar Litter Decomposition in a 18-Year Old Holoptelea Intrigrifolia Planch
Plantation in A Sub-Tropical Forest of Tehri Garhwal
61
11
51 Priyanka Maiti Exploration of Plant Derived Neutraciticals against Lung Cancer Using In
Silico Tools
62
52 Rashi Miglani Ecotoxic Investigations on Agro Ecosystems with Special Reference to
Earthworms
63
53 Renu Suyal Epiphytic Orchids and Their Host Species in Baliyanala Catchment Area of
Nainital, Kumaun Himalaya
64
54 Ripu Daman
Singh
Impact of Forest Fires on Stand Structure and Regeneration 65
55 Shalini Rawat Radiation Absorber Plants 66
56 Surabhi Gumber Fire Adaptations in Some Important Tree Species 67
57 Vinay Singh Study on the Ecological Importance of Aquatic Avian Fauna of Wetlands of
Kumaun Region, India.
68
58 Yogesh Joshi New Horizons in the Field of Lichenology: Case Study from Kumaun
Himalaya
69
59 Bhaskar Ch.
Joshi
Wa Watershed, West Himalaya, India 70
60 Ira Tewari Significance of Community Participation in Promoting the Conservation of
Musk Deer and Its Preferred Fodder in Uttarakhand, In Uttarakhand
Himalaya
71
61 Jagmohan
Singh Negi
Synthesis of Some New Azetidinones Derivatives with Possible
Biological Activities
72
62 Ankita H.
Tripathi
Development of a Reporter System for Screening and
Characterization of Aromatase Inhibiting Potential in the
Phytoextracts/Phytochemicals from Uttarakhand Flora
73
63 Ranjana Singh Genetic Diversity in Black-Seeded Soybean (Glycine max L.)
Landraces of Uttarakhand State
74
64 Pooja Chauhan Taxononomic and Ecological Studies on Catamixis baccharoides
Thomson Endemic Species of Uttarakhand in Dehra Dun & Adjacent
Siwaliks
75
65 Vartika Pant Lichens: A Promising Source of Nutraceuticals and Pharmaceuticals 76
66 S. S. Koranga Age of Rhizome Segments Determine Ramet Regeneration and
Growth of Hedychium spicatum Sm.
77
67 Jyotsna Seed Parameters and Regeneration Potential in Ficus palmate Forssk
in Nainital District of Kumaun
78
68 Babita Joshi Effect of silicon solubilizer in improving the yield and stress tolerance
in rice genotypes
79
69 Prinsa Ramola Biology and Control of Three Problematic Weed Species of Winter
Season
80
70 Priyadarshini
Chanyal
Phytochemical Constituents and Pharmacological Activities of the
Genus Thalictrum (Ranunculaceae): A Review
81
71 Neha Chopra Ethnomedicinally Important Wild Edible Plants of Nainital,
Uttarakhand
82
72 Mamta Bharti Impact of Alien Invasive Species on Soil physiochemical
Characteristics in Sal (Shorea robusta) and Chir pine (Pinus
roxburghii) forest, Uttarakhand, India: A Comparative study
83
12
1. Flow of Forest Ecosystem Services with Special Emphasis on C-Sequestration in a
Mountain Watershed, Kumaun Himalaya
Bharat Giri Gosain
G.B. Pant National Institute of Himalayan Environment and Sustainable Development
(GBPNIHESD) Kosi-Katarmal, Almora 263643, India.
Email: [email protected]
A major problem being faced by human society is that the global temperature is believed to
be rising due to human activities that release CO2 and other greenhouse gases to the
atmosphere and thus causes global warming. In atmosphere CO2 accounts for 0.035% and it
is the most abundant greenhouse gas present in the atmosphere. Besides this other greenhouse
gases responsible for global warming are methane, nitrous oxide, ozone, water vapours and
CFCs. C-sequestration is the process through which agriculture and forestry practices remove
CO2 from the atmosphere. The forests have potential to capture and retain large volumes of
carbon over long periods as trees absorb carbon through photosynthesis process. The present
study was conducted in Syahi Devi forests located in a mountain watershed (Kosi) Almora
district of Uttarakhand, aimed to assessed the potential of C-sequestration rate and Net
Primary Productivity (NPP) of Oak (Q. leucotricophora) and Pine (P. roxburghii) forests at
an altitude of 1780- 2200m. C-sequestration rate was estimated following McDicken (1997)
and Tewari et al. (2008). NPP was following Chaturvedi and Singh (1987) and Rawat and
Singh (1988) by using the allometric equations. Total tree layer biomass in the first year in
Oak forests (mean = 224.664 t/ha) increased in next year (mean = 231.46 t/ha) in 2015.
Average NPP of tree layer of Oak forests in the present study was thus computed to 6.81
t/ha/yr. In Pine forests tree layer biomass (mean = 140.61 t/ha) in year 2014, increased (mean
= 152.4 t/ha) in 2015, thus NPP of Pine forests tree layer was computed to be 11.79 t/ha/yr.
C-sequestration rate in Oak and Pine forests in the present study was recorded 3.23 t/ha/yr
(range = 1.71-3.45 t/ha/yr) and 5.61 t/ha/yr (range = 1.64-6.7 t/ha/yr), respectively.
Key word: C-sequestration, greenhouse gases, net primary productivity, mountain watershed,
Kumaun Himalaya.
13
2. Ecosystem Services in Temperate Sacred Forests of Kumaun Himalaya
Vijay Arya and G. C. S. Negi*
Department of Botany, S.S.J. Campus Almora-263601, Kumaun University, Uttarakhand
G. B. Pant National Institute of Himalayan Environment & Sustainable Development, Kosi-Katarmal,
Almora, Uttarakhand
Email: [email protected]
Sacred forests are a group of trees or a patch of vegetation protected by the local people
through religious beliefs and socio-cultural regulations practices.Uttarakhand has a rich
history of conservation of forests through this approach. The present study was carried out in
eight temperate sacred forests of Pithoragarh district, Kumaun Himalaya situated between
1500-2000 masl.These forests have been offered to the local deity (folk god and goddess) and
declared as “sacred forest” by the local communities either to stop extraction of forest
resources or regulate their use framing well-defined rules and regulations. The eight sacred
forests we studied have been protected for the last 5-10 years. A questionnaire based survey
was conducted in the adjacent villages of these forests to get information about the ecosystem
services provided by these sacred forests. People acknowledged use of various ecosystem
goods and services from these forests and understood the benefits of protecting these forests
which are now providing intangible services such as water, control of soil erosion, wildlife
conservation, moderation of temperature, aesthetic value etc. This paper describes various
mechanisms employed by the rural people to protect their forests through the use of religious
and socio-cultural ethos that is contributing to biodiversity conservation and sustainable
utilization of the forest resources.
Keywords: Ecosystem services, sacred forests, Pithoragarh, Kumaun Himalaya
14
3. Distribution and Diversity of Ocimum Species Occurring in Different Agro- Ecologies
of Uttarakhand State
Rajni Rawat, Ranjana Singh, K.S. Negi†, P.S. Mehta, S. K. Verma and I. S. Bisht ICAR-National Bureau of Plant Genetic Resources, Regional Station, Bhowali (Nainital),
Uttarakhand-263132, India
† Deceased on 14 December 2016
Basil is an aromatic and sacred herb in the family Lamiaceae, used both as a classic culinary,
ornamental and cosmopolitan herb of home gardens. The genus Ocimum contains
approximately 65 species of herbs and shrubs originated in tropical Asia. In the present study
diversity of 79 accessions has been described. Of the 79 accessions, 51 accessions were
collected indigenously from the farmer households of eight districts of Kumaon and Garhwal
region of Uttarakhand state viz. Almora, Bageshwar, Chamoli, Champawat, Nainital, Pauri,
Pithoragarh and Rudrapryag, and 28 accessions were augmented from exotic sources. Six
species of Ocimum namely O. basilicum (six varieties i.e. O.b. var pilosum, O.b. var
basilicum, O.b. var glabratum, O.b. var difforme, O.b. var thyrsiflora and O.b. var
purpurascens), O. tenuiflorum, O. gratissimum, O. Virde, O. kilimandascharium and O.
citriodorum were represented in the study. The accessions were characterized in the Kharif
seasons of 2014-15 and 2015-16 at ICAR-NBPGR Regional Station- Bhowali, Nainital,
Uttarakhand for quantitative and qualitative morphological characters including essential oil
yield content and composition using standardized descriptors for identification of superior
genotypes. Wide variations were recorded for different quantitative characters such as spike
length, plant height, herbage yield, oil percentage and major component of essential oil.
Spike length varied from 5.1-33.2 cm, plant height varied from 50.5- 141 cm, herbage yield
varied from 89- 516 g, oil percentage varied from 0.7- 2.47%. Methyl chavicol, linalool, β-
cedrene, β- bisabolene, 1,8- cineole and eugenol were recorded as major compound of above
mentioned Ocimum species. Some promising accessions identified for major compound in
different species include, O. b. var. pilosum (EC 387834) for methyl chavicol (88.6 %); O. b.
var. basilicum (EC 333788) for linalool (82.4%); O. virdie (NRT-05) for β bisabolene
(30.25%); O. kilimandscharicum (IC 449493) for camphor (45.58%), and O. tenuiflorum
(RNRT- 26) for eugenol (70.52%). Promising accessions for herbage yield include O. b. var.
basilicum (EC 388889, 710 g) and O. citriodorum (NRT- 03, 638.3 g). These promising
accessions have potential for commercial cultivation in different parts of Uttarakhand hills.
Keywords: essential oil, Ocimum, quantitative genetic variations, species
15
4. Allelopathic Effect of Leaf Extract of (Poplar Deltoidus) in Seed Germination, Root
Characters and Physiology of Rice Seedlings
Kamla Dhyani, Namrata Singh, Chhaya Singh and Shweta Sahni
Shri Guru Ram Rai Institute of Technology & Sciences, Patel Nagar Dehradun, Uttrakhand
Email: [email protected]
In the present investigation an attempt to be made to analyse the potential allelopathic effect
of Poplar deltoidus leaf extract at different concentrations (0, 1, 2, 5 and 10 %) of water
extract of green and decomposed leaves of Poplar deltoidus. On seed germination, seedling
growth and biochemical constituents changes in Oryza sativa L. The results showed that the
both leaves extracts of Poplar caused a general phytotoxic effect on seed germination and
seedling growth of O. Sativa at some concentration like 10 % and 5 % and some stimulatory
effect on some lower concentrations (1% and 2 %). It was noticed that the germination % in
both the extract is inhibited in higher concentration. The degree of seed germination
inhibition was higher in green leaf extract as compare to decomposed leaf more than. It was
also noticed that root morphology is highly effected by the treatment extensive secondary
root growth was observed in lower concentration extract as compare to the control.
Allelopathic effect on germination percentage is not significant but the other growth
parameters like shoot length, root length, dry weight, chlorophyll content of the leaves and
proline content is highly effected.
16
5. Development and Application of SCAR Markers for Identification of Gladiolus
Germplasm
Niraj Singh1, Ashish K. Pal1, Baleshwar Meena1, R. K. Roy1, Sushma Tamta2 and T. S.
Rana1
1CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow -226001, India 2Department of Botany, D. S. B. Campus, Kumaun University, Nainital-263001, Uttarakhand, India
Email: [email protected]
Gladiolus is an economically important ornamental crop, cultivated for its beautiful flowers
throughout the world. The genus Gladiolus consists of about 265 species and is one of the
largest genera in the family Iridaceae. As the number of Gladiolus cultivars are increasing
day by day in India, therefore, it is very significant to develop cultivar specific markers for
rapid identification. The correct genotype identification of plant material is very significant in
the floriculture industry. The aim of this study was to develop sequence-characterized
amplified region (SCAR) markers from the RAPD and ISSR fragments for identification and
authentication of Gladiolus germplasm. The SCAR markers developed could be easily
employed as valuable tools to identify newly developed Gladiolus cultivars. The SCAR
markers viz., ScG12, ScG34 and ScG36 are specific to the DNAs from all sixty two
Gladiolus cultivars, as they did not amplify the DNAs of other taxa of the family Iridaceae,
including Iris, Amaryllis, and Narcissus. All three SCAR markers distinguished Gladiolus
from other taxa of the family Iridaceae, whereas marker ScAm was specific to ‘Amethyst’
cultivar. Our results confirmed that this particular SCAR marker distinguished ‘Amethyst’
cultivar from rest of other sixty two Gladiolus cultivars investigated in the present study. The
development of SCAR markers based on RAPD and ISSR markers seems to be the maiden
attempt for Gladiolus cultivars.
Keywords: Gladiolus, identification, ISSR, RAPD, SCAR marker
17
6. Rapid Vulnerability Assessment of Trillium Govanianum Wall. Ex D. Don; A
Commercially Exploited Medicinal Plant of Himalaya
Harsh Kumar Chauhan1,2, Anil Kumar Bisht1, Indra Dutt Bhatt2,
1Department of Botany, D.S.B. Campus, Kumaun University, Nainital-263001, India 2 G.B. Pant National Institute of Himalayan Environment & Sustainable Development, Kosi-Katarmal,
Almora- 263643, India
Email: [email protected]
Trillium govanianum Wall. ex D. Don (Nagchhatri) is an endemic Himalayan medicinal plant
restricted to shady, moist and cold habitat in subalpine zones at 2400-3500 m asl. Trade of its
rhizomes collected from wild populations has recently emerged as a significant income
source in Himalayan region. The rhizome of the species is reported to have rich amount of
steroidal saponins and have potent uses in cancer, reproductive disorders and inflammation.
We assessed ecological vulnerabilities in 17 sites from Tirthan Valley (Himachal Pradesh),
Munsiyari and Tunghnath (Uttarakhand); and socio-economic vulnerabilities through
communal focus groups and interviews with 579 gatherers and 19 traders. Species
vulnerability was recorded highest in Munsiyari, then Tunghnath, and indicated that the
species may reach at the brink of extinction in near future. Recommendations to encourage
sustainable utilization are suggested with the aim to reduce the threat of extinctions while
maintaining community incomes.
Keywords: Ecological vulnerabilities, socio-economic vulnerabilities, steroidal saponins,
trade, vulnerability
18
7. Impact of Environmental Factors on the Growth of Plants
Indu Tiwari
Department of Botany, Government P. G. College, New Tehri, Uttarakhand
Email: [email protected]
Plant growth and distribution are limited by the environment. If any one of the environmental
factors is less than ideal it will become a limiting factor in plant growth. Limiting factors are
also responsible for the geography of plant distribution. For example, only plants adapted to
limited amounts of water can live in deserts. Most plant problems are caused by
environmental stress, either directly or indirectly. Therefore, it is important to understand the
environmental aspects that affect plant growth.
Environmental factors viz., light, temperature, water, and soil, greatly influence plant growth
and geographic distribution. These factors determine the suitability of a plant for a particular
location, management practices, and levels of inputs needed. A plant performs best and is
least costly to propagate if it is grown under the most favorable environmental conditions. To
maximize the production of any plant, it is important to understand how these environmental
factors affect plant growth and development.
Keywords: Limiting factors, environmental stress, management practices, geographic
distribution, propagate
19
8. Observation on Status of Insect Pollinators in Binsar Wildlife Sanctuary, Western
Himalayas, Uttarakhand, India
Prachi Tamta, Manoj K. Arya and Dayakrishna
Department of Zoology, D.S.B. Campus, Kumaun University, Uttarakhand, India
Email: [email protected]
Present investigations were undertaken to know the status of insect pollinators in Binsar Wildlife
Sanctuary (BWLS), Kumaun Himalayas, Uttarakhand, India. The geographical location of BWLS
is 29o39’-29o44’N and 79o41’-79o49’E and the altitude varies between 1200 to 2500 meters above
sea level. BWLS represents the characteristic floral elements of Moist Temperate Forest
surrounded by Chir pine and agricultural land. Insect as a pollinator has important role to maintain
biodiversity in natural ecosystem. Insects including honey bees, bumble bees, butterflies, moths
and beetles are vital for the pollination of many cultivated and wild plants. A total of 53 species of
insects were found to facilitate the pollination process in the BWLS during the study period 2013-
15. The identified pollinator species of insects belonged to order Lepidoptera (33 species),
Hymenoptera (11 species), Diptera (five species) and Coleoptera (four species), respectively.
Among the four recoded group of insects, Lepidopteran insects belonging to families
Nymphalidae, Pieridae, Papilionidae, Lycaenidae, Danaidae, Satyridae, Acraeidae, Arctiidae,
Noctuidae and Sphingidae were recorded in great numbers indicating efficient pollination in the
study area. Similarly, Hymenopteran insects belonging to families Apidae, Scoliidae, Pompilidae,
Vespidae and Xylocopidae were recorded in great numbers indicating efficient pollination in the
study area. Among the Hymenopteran pollinator insect species, bees (Anthophora confusa Smith
and Apis laboriosa Smith), bumble bees (Bombus sp. and Bremus sp.), and Xylocopa fenestrata
Fabricius were recorded as the most frequent flower visiting and efficient pollinator insect species
during the field visits in BWLS.
Key words: Hymenopteran, insects, lepidopteran, pollinators, species
20
9. QSAR Based Virtual Screening of Novel Phytochemical Inhibitors Against HER2 in
Breast Cancer
Mahesha Nand1, Priyanka Maiti2, Subhash Chandra2* and Veena Pande1
1Department of Biotechnology, Kumaun University, Bhimtal Campus, Bhimtal, Uttarakhand, India. 2Department of Botany, Kumaun University, S.S.J Campus, Almora, Uttarakhand, India.
Email: [email protected]
Breast cancer is the second most widespread cancer in the world among women with an
estimated 1.67 million new cancer cases diagnosed in 2012 (25% of all cancers). It is the
most frequent cancer in women both in more and less developed regions with slightly more
cases in less developed (883,000 cases) than in more developed (794,000) regions. In Indian
yearly incidence of breast cancer were approximately 1, 44,000. In Uttarakhand around 25%
patients were diagnosed with breast cancer.HER2 (human epidermal growth factor receptor
2) receptor is a membrane tyrosine kinase and when activated affects cell proliferation and
survival. HER2 amplification is the primary pathway of HER2 receptor overexpression and is
a major driver of tumor development and progression in a subset of breast cancers. HER2
amplification is found in case of 15% to 20% breast cancer. Therefore overexpressed HER2
receptor is an importantdrug target and the present investigation aims to explore some novel
phytochemicals against HER2 by applying QSAR based virtual screening methods. Screened
phytochemicals and their family are includes Cisplatin from Zanthoxylumarmatum,
Homoharringtonine from Ulmusparvifolia, Daidzein from Puerariamirifica, Genistein from
Puerarialobata and Catechins from Camellia sinensis. Screening anti-cancer compounds
from several medicinal plants and natural compounds is attractive because they are safe,
nontoxic and cost effective. The results of the work have identified several novel anti-cancer
phytochemicals against breast cancer with future avenues in drug development against breast
cancer.
Keywords: Breast cancer, phytochemicals, machine learning, her2, breast cancer, text
mining.
21
10. Biochemical Aspects of Photosynthesis and Respiration: An Implication to
Environment and Social Awareness
Preeti Bhatt and Chandra Kala Pant
Department of Chemistry, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand, India
Biochemical reactions in living systems regulate carbon dioxide (CO2) and oxygen (O2)
balancein the atmosphere. Photosynthetic processes are highly significant in minimizing CO2
level while the glycolytic pathways along with electron transport, producing CO2 and water
(H2O) at the expense of O2, through respiration are widely known. In this presentation the
biochemical aspects of respiration and photosynthesis has been considered to understand the
consequences of these metabolic processes. Keeping in view the hazardous effects of
deforestation and diseases caused by excessive intake of food materials (carbohydrates,
proteins, fatty acids etc.) and alcoholism are discussed and their correlation with the
environment and social awareness has been manifested.
Keywords: Photosynthesis, respiration, environment, deforestation, health awareness
22
11. Analysis of Forest Vegetation, Population Structure and Regeneration Pattern in
Kumaun Himalaya
Meenakshi Negi and R. S. Rawal
G. B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-
Katarmal, Almora 263643, Uttarakhand, India
The noticeable decline in natural regeneration is of great concern for west Himalayan forests
due to several reasons, like, excessive lopping, over grazing and tree felling, non-viable
seeds, extreme weevil and pest infestation, animal and bird predation resulting in low seed
production. A study was therefore, carried out with an objective to quantitatively analyse
representative forests around Nainital to record patterns of natural regeneration. Considering
the dominance, a total of six forest types, P. roxburghii, mixed oak, Q. leucotrichophora, Q.
lanuginosa, Q. floribunda and Q. semecarpifolia were identified. The tree density of these
forests ranged from 325-2534 ind/ha, sapling density from 258-1733 ind/ha and total tree
basal cover from 25.29-109.37 m2/ha. Seedling density for both the years of observation was
found maximum in Q. floribunda forest in rainy season i.e. 3765 ind/ha (I year) and 3491
ind/ha (II year). Surprisingly, no seedlings were recorded from Q. semecarpifolia forest. The
composition of tree and shrub layers differed markedly among all the forests. Q. floribunda
forest supported the largest shrub population, while Q. semecarpifolia was poorest in this
regard. With an exception of Q. semecarpifolia, the dominant species in all the forests had a
stable population indicating good regeneration. The paper attempts to describe future
possibilities of management in these forests.
Keywords: Kumaun Himalaya, oak forests, phytosociology, population structure,
regeneration
23
12. A Seasonal (Rainy Season) Survey and Study on the Availability of Different Fodder
Species of Kumaun and Garhwal Region of Uttarakhand
Hemlata Rathore, Netrapal Sharma and Satpal Singh Bisht
Department of Zoology, D. S. B. Campus, Kumaun University, Nainital-263001.
Email: [email protected]
Uttarakhand is a state which consists of 65% of forest cover and 86% region is mountainous
It is surrounded by Himalaya from the northern side which provides a remarkable range of
variation among the species the area consist of alpine zone and tropical forest. Trees leaves,
shrubs, herbs, legumes and grasses are used as a fodder by the local villagers. Despite being a
biodiversity rich state it is observed that there is an acute deficiency of quality fodder for the
livestock. In order to check the availability of fodder species and their nutrient value a
seasonal survey was done across the Kumaun and Garhwal region of Uttarakhand and also
their availability was observed along different elevation. The survey was done approx in
every district of Kumaun and Garhwal region.The fodder species collection is 480 and 390 in
Kumaun and Garhwal region respectively. Dudhila (Ficus nemoralis), Eleusine indica
,Bhimal (Grewia optiva), Sakina (Indigofera), Timil (ficus roxburghii),Tusharu (Debregeasia
spp.) Doob (cynodon dactylon) are found to be most common according to availability during
rainy season. The survey also highlighted the fact that there is lack of awareness among the
villagers regarding the quality fodder and its management. The survey can help in identifying
the fodder species rich area and also provide options for better fodder management
techniques. Further awareness of quality fodder among villagers can slightly improves the
deficit of fodder for the livestock.
Keywords: Availability, deficit, Fodder, livestock
24
13. Allelopathic Effect of Bryophytes on Germination and Growth of Bidens Biternata
(Lour.) Merr & Shreff
Prabha B. Dhondiyal, Neerja Pande and Kiran Bargali
Department of Botany, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand, India
Email: [email protected]
The present work embodies the allelopathic effect of bryophytes (Lunularia cruciata,
Marchantia polymorpha and Plagiochasma appendiculatum) on the germination behaviour
and biomass of Bidens biternata. The aim of the work was to study the effect of bryophyte
species on unsoaked and presoaked seeds of B. biternata. The extract of these bryophytes
were prepared in different organic solvents of varied polarity (acetone, ethanol and water) at
two concentrations (100% & 50%). For pot experiments bryophytes were mixed with soil and
as layer on the top of the soil. In in vitro and pot experiment the bryophytes had different
degree of inhibitory effect on the germination and biomass of B. biternata as compared to
control. The germination was completely inhibited in unsoaked seeds of Bidens in acetone
and ethanol extracts of bryophytes at both the concentrations. In presoaked seeds of Bidens
the germination was completely checked at higher concentration of acetone and ethanol
extracts. The initiation of germination was also delayed in bryophytic extracts. The radicle
length, plumule length, total seedling size and biomass varied between bryophyte species,
solvent and concentration of the solvent used.
Keywords: Allelopathy, bryophyte, Bidens, germination, inhibition, weed
25
14. Genetic Diversity among Sea Buckthorn (Hippophae Rhamnoides (L)) Using Molecular
Marker Based Analysis
Netrapal Sharma1, Satpal Singh Bisht1, Hemlata Rathore1 and Sanjay Gupta2
1Department of zoology, D.S.B. Campus, Kumaun University, Nainital-263001 2SBS PG Institute, Balawala, Dehradun, Uttarakhand-248161
Email: [email protected]
The genetic diversity among thirty six genotypes of Hippophae Salicifolia D. Don of
Uttarakhand region and Hippophae rhamnoides subsp. Turkestanica of Ladakh region were
studied; using simple sequence repeats DNA (SSRs)-PCR, Random amplified primers
(RAPD) and Cytochrome P450 analysis. The protein profiling and antioxidant profiling
(quantitative and qualitative) of seeds were also done to select the superior genotypes having
high economic importance. A total of seven SSR, twenty one RAPD and five CytoP450
primers were used for detecting genetic variability in Sea buckthorn seeds out of which seven
SSR, eleven RAPD and five CytoP450 primers were highly reproducible and found suitable
for use in PCR (Polymerase Chain Reaction) analysis. SSRs profile obtained by primer UTR-
15 was containing total of 8 bands (0.15- 0.6 kb), among these eight bands, one was found to
be polymorphic (12.5%). The similarity coefficients based on SSR markers ranged from
0.011 to 0.999 with an average value of 0.226. The polymorphism information content (PIC)
value ranged from 0.1686 (UTR-015) to 0.2975 (UTR-12) with a mean of 0.351. In RAPD, a
total of 198 bands were scored corresponding to an average of 10.8 bands per primer with
138 bands showing polymorphism (73.2 %) with similarity coefficient ranging from 0.011 to
0.999. A dendrogram constructed based on the Unweighted Pair Group Method with
Arithmetic Mean (UPGMA) clustering method revealed two major clusters of Uttarakhand
(with sub-clusters) and Ladakh region (no sub-clusters). This study may useful in the
selection of superior genotypes having higher nutraceutical value by using developed
molecular and protein based markers in Uttarakhand and Ladakh regions.
Key Words: Elaeagnaceae, Hippophae salicifolia, genotypes, sea buckthorn, genetic
diversity
26
15. Impact of Lichen Litter on Soil Nutrients in the Temperate Areas of Kumaun
Himalaya (Uttarakhand)
Balwant Kumar and Mahesh Kumar
Department of Botany, Kumaun University, S.S.J. Campus Almora, Uttarakhand, India
Email: [email protected]
Fresh lichen litter- Usnea, Everniastrum and Parmotrema were removed from Quercus twigs
situated in temperate regions of Kumaun Himalaya. Agricultural soil samples were also
collected for their chemical analysis and lichen decomposition activity. Individuals of
selected lichens were mixed separately with this soil. Total fifteen sample buckets were used
for lichen decomposition purpose. After six months of decomposition, observations were
taken. Usnea was found to be the fastest decomposed lichen (about 91%) and increased the
value of soil macronutrients- pH, N, P & K. It is the first attempt regarding impact of lichen
decomposition on soil nutrients in the area.
Key words: Kumaun Himalaya, lichen litter, decomposition, Usnea, soil nutrients
27
16. Adsorptive Removal of Zinc (II) by Activated Bark of Pinus Roxburghii : An
Ecofriendly Approach
Mahesh Chandra Arya, Shah Raj Ali and Rajesh Kumar
Chemistry Department, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand 263002, India
Email: [email protected]
This work highlights an inexpensive, sustainable and ecofriendly adsorbent for the removal
of Zinc (II) from its aqueous solution using activated bark powder of Pinus roxburghii as an
adsorbent. Pine bark was collected from local Pinus roxburghii trees, also known as chir
pine. The washed bark was oven-dried, ground and sieved through the mesh of different sizes
ranging from 100µm to 150 µm. The powder was treated with HCl, NaOH and water till it
got stabilised in aqueous medium. It was activated by heating it at 1000C for suitable time
and used as adsorbent to remove Zinc (II) from its aqueous solution. The adsorptive removal
was studied in the concentration range 50 to150 mgL-1.The various parameters studied were
effect of contact time between adsorbate and adsorbent, pH, concentration of adsorbate, size
of particles of adsorbent, amount of adsorbent and adsorption isotherm. The activated bark
powder of Pinus roxburghii showed maximum efficiency of 88% at optimum pH of 4.0 and
contact time of 300 min for an initial concentration of 100 mgL-1. The data obtained was
found to follow Langmuir adsorption isotherm model. The work demonstrates the removal of
Zinc (II) from its aqueous solution efficiently using pine bark powder as an adsorbent.
Keywords: Zinc (II), adsorption, pine bark
28
17. Observation on Environmental Effect on the Breeding Dynamics of Schizothorax
Richardsonii (Gray), from Kumaun Himalayas, Uttarakhand, India.
Uzma Siddiqui and H. C. S. Bisht
Department of Zoology, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand, India
Email: [email protected]
Studies of environmental factors of an aquatic ecosystem are basic for understanding its
breeding pattern. In aquatic system, the successful reproduction is based on sensing the
environmental factors. Each factor plays an individual role but it is the synergistic effect of
various environmental parameters which determines its breeding dynamics. Due to
environmental factor such as temperature the breeding pattern is change in comparison to
earlier period. pH is also increased due to forest fire and heavily construction of houses and
roads. In Kumaun region of Uttarakhand. Dissolved Oxygen and free Carbon dioxide is also
effected which affects the breeding biology of snow trout, Schizothorax richardsonii.
Dissolved Oxygen is decreased during summer due to smog and smoke of forest fire. It is
very surprising observation that the Nainital Lake had very rich population of Schizothorax
richardsonii in the earlier but in present scenario there is no Schizothrax richardsonii at all in
Nainital Lake except the exotic carp are growing. The breeding dynamics of this species
depends on these environmental factors. Through the management practices for fish culture
snow trout can be a successful breeder of Kumaun river by utilising scientific tools for
fisheries.
Key words: Breeding, dynamics, environmental parameters, reproduction, temperature
29
18. Antimalarial Activities of Some Plant Species Found in Kumaun Region
Uttarakhand
Pushpa Bhakuni
Department of Chemistry, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand
Malaria is a vector borne disease prevalent in tropical and developing countries causing
significant morbidity and mortality. According to the latest estimates of WHO, in 2015 there
were about 214 million cases of malaria worldwide and an estimated 438000 deaths
attributable to it. Plasmodium falciparum (Pf), the most deadly malaria causing organism,
has developed resistance to most commonly used drugs classes viz. the cinchona alkaloids,
4-aminoquinolines and the sulphonamides+diaminopyrimidines. After widespread resistance
to commonly used antimalarial drugs viz. Quinine, Chloroquine and
sulphadoxine+pyrimethamine, the Artemisinin derivatives became the only hope to fight
against Malaria. However, alarming signals of Artemisinin resistance emanating from areas
adjoining Cambodia are causing fears of rapid spread of Artemisinin resistance, leaving
humanity to suffer this dreaded disease without a vaccine or effective and affordable
antimalarial drugs. Therefore, there is an urgent need to search for novel antimalarials on a
war footing. Plants have been used in the traditional medicine for the treatment of malarial
fever and have indeed provided us two of the most powerful antimalarial drugs viz, Quinine
(from cinchona bark) and Artemisinin (from Artemisia annua).
Kumaun region in Uttarakhand state in the Indian Himalayan region is geographically and
climatically diverse with number of plant species known for their medicinal properties. A
Survey of ethnobotanical literatures reveal use of many locally found plant parts as traditional
remedies.
The treatment of various ailments including malarial fever. Such plant species are
Achyranthes aspera, Ajuga bracteosa or parviflora, Berberis aristata or asiatica, Nepeta
leucophylla, Pongamia pinnata, Roylea cinerea, Sida spinosa and Vitex nigundo found in
Kumaun region. Therefore there is an urgent need of evaluation of biologically active
compound against malaria from all these plant species which may be an alternative and
effective antimalarial agent. This may help to decrease the burden of the disease and prevent
emergence of resistance to currently used antimalarials.
30
19. A Review of Chemical Analyses and Ethno-medicinal Uses of Zingiberaceae Family
Sapna, Pushpa Joshi and Manoj Dhuni
Department of Chemistry, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand, India.
Email: [email protected]
Zingiberaceae family is an important natural resource for food, perfumes, dyes, medicines,
spices and aesthetics. India is one of richest and diverse region for Zingiberaceae having 22
genera and about 170 species, where North-east region is a zone of greatest concentration
having about 19 genera and about 88 species. Natural products from plants of this family find
application in several therapeutic formulations. These compounds belong to different
chemical classes (alkaloids, terpenoids etc.) and have chemically diverse and complex
structures. The rhizomes of plants belonging to this family are aromatic, tonic and stimulant.
They are rich sources of essential oils which exhibit various physiological activities,
antimicrobial, anti-arthritic, antioxidant, anticancer, anti-inflammatory, anti-diabetic, anti-
HIV, neuro-protective etc.
The objective of this paper is to make a collection and summarize the researches conducted in
Zingiberaceae family so that researcher and academician may have better understanding
about variations in chemical properties due to different region, climate and geographical
conditions. The second objective of this paper is to identify the gap in the researches and to
bridge the gaps.
Keywords: Zingiberaceae, rhizomes, natural products, physiological activities
31
20. Effect of Zinc on Growth and Herbage Yield of Ocimum Gratissimum L.
Mamta Bisht and Geeta Tewari
Department of Chemistry, D. S. B. Campus, Kumaun University, Nainital 263002
Email: [email protected]
The genus Ocimum, (family Lamiaceae) is a rich source of aroma chemicals that find varied
application in the food, flavor and pharmaceutical industry. Zinc is an essential micronutrient
for the growth and reproduction of plants and active site of various enzymes that are
responsible for driving many metabolic reactions in all crops. Growth and development
would stop if specific enzymes were not present in plant tissue. Protein, and chlorophyll
formation is significantly reduced in zinc-deficient plants. Therefore, a constant and
continuous supply of zinc is needed for optimum growth and maximum yield. The purpose of
this study is to determine the effect of zinc on growth and yield of Ocimum gratissimum L. A
polyhouse study was conducted to determine the effect of zinc amendment on the growth and
yield of Ocimum gratissimum L.The treatments of zinc were 600, 1000 and 1200 mg kg-1 in
triplicate along with un-amended control .On increasing amendments there is a significant
decrease in growth ( height and weight) of the plant, while yield increases significantly with
unamended control. From the above study, it is suggested that this Ocimum species which
finds wide application in herbal products, can be cultivated in zinc contaminated soil for its
volatile constituents.
Keywords: Ocimum gratissimum L., growth, oil yield, zinc
32
21. Chemotypic Variations in Ageratum Conyzoides L. Collected From Indo-Gangetic
Plains
Bhanu Kumar1, Ankita Misra1, Sharad Srivastava1, Yashwant Singh Rawat2 and Ajay
Kumar Singh Rawat1 1Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute,
Lucknow (Uttar Pradesh)
2Botany Department, Kumaun University, Nainital (Uttarakhand)
Email: [email protected]
The present study investigates the chemotypic variations in germplasms of Ageratum
conyzoides L., collected from Indo Gangetic Plains. Twenty-one germplasms were evaluated
for morpho-anatomic variations and chemoprofiling through HPTLC. The morphological and
anatomical variations were slight; however, quantification of biologically active marker
compounds Precocene I (PI) and Precocene II (PII) using Toluene-Ethyl Acetate (9.8: 0.2) as
solvent system revealed significant variation among germplasms. Precocene I and Precocene
II content ranges from 0.001 to 0.082% and 0.001 to 0.078% respectively on dry weight
basis. Cluster analysis based on the correlation matrix of metabolites, bifurcate the
germplasms into two groups, separating NAC-13 with a considerable amount of both
Precocene I and Precocene II. It is noteworthy that a negative correlation (Karl Pearson
coefficient = -0.2329) was observed between the two marker compounds. The results clearly
depict the variations in metabolite content among the intraspecific germplasms. Five elite
germplasms were identified which can be utilised for commercial prospection for the
development of herbal formulation with the desired effect in near future.
Keywords: Ageratum conyzoides L., HPTLC, Indo-Gangetic plain, Precocene-I, Precocene-
II
33
22. Essential Oil Composition of Murraya Koenigii Collected from Three Locations of
North India
Akanksha Rani and Chitra Pande
Department of Chemistry, D. S. B. Campus, Kumaun University, Nainital 263001, Uttarakhand,
India
Email: [email protected]
Murraya koenigii (L.) Spreng (family Rutaceae), shows many therapeutic properties such as
anti-inflammatory, antidiarrheal, antifungal, radioprotective, chemoprotective, analgesic,
anti-diabetic, anti-oxidant, memory enhancer, anti-helminthic, anti-bacterial and anti-cancer.
The essential oil of fresh leaves collected from three Indian locations has been analysed by
Gas Chromatography and Gas Chromatography-Mass Spectrometry. The analyses revealed
the presence of 41-62 compounds. The main compounds in the sample from Ramnagar were,
α-pinene (30.2%), sabinene (23.5%) and (E)-caryophyllene (12.1%) while the major
compounds in the sample collected from Betalghat were α-pinene (41.4%), sabinene (24.5%).
The major compounds found in the sample of Bijnor were α-pinene (40.8%) and (E)-
caryophyllene (10.5%). The volatiles of the plant from all three locations are rich in
hydrocarbons which are highly valued for their anti-inflammatory, antimicrobial, neuro-
inflammation, anti-bacterial and anti-fungal activities.
Keywords: Murraya koenigii, α-pinene, sabinene, (E)-caryophyllene, essential oil
34
23. Physicochemical Characterization of Soil Collected From Sacred and Non Sacred
Forests of Uttarakhand: A Comparative Study
Brij M. Upreti and Lalit M.Tewari
Department of Botany, D. S. B. Campus, Kumaun University, Nainital
Email: [email protected]
Sacred forest plays a crucial role in soil and water conservation due to their high biodiversity.
With a rapid litter decomposition rate, nutrient release in to the soil of these groves is very
high nutrient ratio. The soil itself has little nutrients to support the large biomass of the sacred
grove. The fine root mat developed on the surface of the soil is important for supporting the
large above-ground biomass and for effective recycling of nutrients. The present study was
undertaken to understand soil properties in relation to traditionally protected and non
protected two forests structures “Kalika Sacred forest” and “Chamunda Devi sacred forest”
Uttarakhand.
Walkley and Black’s rapid titration method as modified by Walkley (1947) was adopted for
organic carbon estimation. Available phosphorus was determined in the soil by Olsen
method. Potassium was extracted by neutral normal ammonium acetate method and was
determined by the flame photometer. Total nitrogen was measured by using the standard
Kjeldhal procedure.
Soil samples were collected from Kalika devi and Chamunda Devi sacred forest and non
sacred forest from different depth viz.0-10, 11-30, 31-60 and 61-90 cm. Physiochemical
analysis comprises soil texture (sand 47.88%, silt 39.51% and clay 42.46%, soil moisture
(24.24%), Water holding capacity (54.34%), Bulk density (1.42 g/cm-3), porosity(52.08), pH
(6.6), Organic matter(3.86), Carbon(2.24%), nitrogen (274.72 Kg/h.), phosphorous (0.014%)
and potassium(0.025). While in Chamunda devi sacred forest soil texture (sand 42.91%, silt
33.02% and clay 41.85%), soil moisture (26.27%), water holding capacity (60.9%), bulk
density (1.29 gcm-3), porosity (53.10%), pH (4.8), Carbon (1.45%), nitrogen (158.04 Kg/h),
phosphorous (0.0021%) and potassium (0.003%). Physiochemical results showed that sacred
forests had nutrient rich soil comparatively non sacred forest.
Keywords: Sacred forest, non sacred forests, organic carbon, soil properties
35
24. Confirmation of Interacting Partners of Chickpea DNA Methytransferases Clones
and Their Interaction with AGO Protein
Vandana Jaggi1, Sneha Tewari, 1 and Chetan Sharma2
1G.B Pant University of Agriculture and Technology, Pantnagar 1 National Institute of Plant Genome Research, New Delhi
2 Maharishi Markandeshwar University, Ambala
E-mail: [email protected]
A conserved epigenetic modification, DNA methylation involved in many biological
processes. It plays a crucial role in development of inheritable gene silencing. The three
important DNA methyltransferases (DNAMTases) reported in plants, namely
Methyltransferase (MET), Chromomethylase (CMT) and Domains Rearranged
Methyltransferase (DRM) maintain methylation mainly at CG, CHG and CHH sites. It
regulates the gene expression pattern, genome stability in plants and also controls
transcription of invading and mobile DNA elements, such as transgenes, viruses, transposons
and retroelements. It has been demonstrated that mutation in methyltransferases gene results
in elimination of methylation throughout the genome. However, to our knowledge, no more
studies has been reported the analysis of DNA MTases in legumes, so far. In present study
the interacting partners of DNAMTases have been identified through yeast two hybrid
analysis as well as the interaction of AGO protein with DNAMTase checked. For this
plasmid DNA was isolated from pDEST22 harboring DNAMTases clones, confirmed
through restriction digestion with BSrG1 followed by sequencing. To find the interacting
partners yeast competent cells were prepared using yeast strain AH109 as host for bait
pDEST32 and prey pDEST22. The pDEST22 harboring DNAMTases clones were
transformed into yeast competent cells and positive clones were identified by growing in SD-
Trp media. However the competent cell harboring prey was prepared and bait harboring
cDNA clones of chickpea was transformed and confirmed positive clones were selected by
growing on SD-Leu-Trp medium followed by X-gal screening. Further, to check the
interaction of AGO protein with DNAMTases, the AGO protein was cloned in TOPO entry
vector followed by LR ligation in pDESTGADT7. Yeast competent cells using strain
MaV203 were prepared and bait vector pDESTGADT7 harboring AGO and prey vector
pDESTGBKT7 harboring DNAMTases were cloned. Positive clones were selected on
medium SD-Leu-Trp as well as by colony PCR. This study confirmed that the DNAMTases
identified in chickpea regulate the gene methylation by using AGO protein.
Keywords: DNAMTases, AGO, pDEST22, pDEST32, MaV203
36
25. Heavy Metal Accumulation by Coriandrum Sativum in Cow Dung Amended Soils
Deepshekha Punetha and Geeta Tewari
Department of Chemistry, Kumaun University, Nainital, Uttarakhand, 263002, India
Email: [email protected]
Coriandrum sativum L. (Family Apiaceae), an aromatic annual herb is extensively cultivated
for its leaves, seeds and essential oil.
A greenhouse pot experiment was conducted to evaluate the effect of manure (cow
dung) on the accumulation of Zn, Cr, Cu and Ni present in the contaminated soil. The soil
was amended with manure at four levels (0 t/h; control, 26, 52 and 104t/h). After 90 days,
heavy metals accumulation in roots and translocation within coriander grown on these soils
were examined. The soil samples were digested using aqua-regia method and DTPA
extraction method, while plant samples were digested using Tri acid.
All manure treatments (26, 52 and 104t/h) showed a decrease in plant and seed
weight, while plant height, pH and EC of soil were increased. With the increase in manure
concentration, accumulation of different metals decreased significantly. The contents of all
metals were lowest in the 104 t/h treatment. This reduction in metal accumulation was 15.2,
12.4, 35.4 and 5.5 % for Zn, Ni, Cu and Cr in the first cutting and 9.0, 16.9, 27.2 and 3.9%
for the second cutting at 104t/h respectively. Copper and zinc transfer was higher in the aerial
parts as compared to the roots, whereas Ni and Cr were mainly accumulated in roots as
shown by translocation and bioconcentration factor. Coriandrum sativum may be cultivated
on polluted soils for green strategies to obtain essential oil used as flavoring agent in food
products, perfumes and cosmetics instead of using the plant for culinary purposes. Whereas,
Coriandrum sativum grown in manure amended soils can be used for culinary purpose.
Keywords: Manure, zinc, copper, nickel, chromium, translocation, bioconcentration factor
37
26. Oven Dried Volatile Constituents of Ocimum americanum: A Rich Source of
Bioactive Compounds
Sunita Bhatt and ChitraPande
Department of Chemistry, D. S. B. Campus, Kumaun University, Nainital
Email: [email protected]
Food-borne diseases growing worldwide very rapidly. Use of various preservation practices
for successful control of food-borne pathogens is needed for storage of food products.
Synthetic food preservatives have several side effects which enhance the need of natural
preservatives to extend the shelf life of a product. Ocimum americanum L. belonging to the
family Lamiaceae is a wild aromatic herb that is native to tropical Africa. The herb is useful
for its anti-diabetic, antioxidant and antimicrobial activities.The leaf contains the essential oil
of therapeutic significance. Fresh O. americanum plants were harvested at flowering stage
from Ranikhet, Uttarakhand and a part was oven dried at 45˚C. Aerial part of fresh and oven
dried plants was hydrodistilled using Clevenger apparatus. The oil samples were analyzed by
Gas Chromatography and Gas Chromatography-Mass Spectrometry (GC and GC-MS).The
percentage of methyl chavicol and caryophyllene oxide increases and of β-bisabolene and
(E)-γ-bisabolene decreases in dried sample. Concentration of 1,8-cineole becomes double
after drying which has a significant antimicrobial and antioxidant activity besides enhancing
the flavor and fragrance of the food stuff. The dried plant material can be used as a natural
preservative for increasing the shelf life of packaged products.
Keywords: Ocimum americanumL., drying, 1,8-cineole, preservatives.
38
27. Changes in Biodiversity and Regeneration Pattern in Plant Communities after Eradication of
Lantana Camara L. From the Forest Areas of Uttarakhand
Jitendra Bhatt
Department of Forestry and Environmental Science, D. S. B. Campus, Nainital
Email: [email protected]
Preservation of native diversity is one of the biggest challenges in Uttarakhand. Invasive non-
native species are a part of this challenge because they actively contribute to diminish native
biota after disturbance and land use change. Often referred to as ‘biological pollution’ these
non-native invasive species can be found in all taxon levels. In the Shiwaliks and lesser
Himalayas Lantana camara, Parthenium hysterophorus, Ageratum conyzoides and Ageratina
adenophora are the main invasive alien species. Lantana camara L. (Kuri), Mexican native,
is introduced in India as an ornamental and live fence. Keeping the detrimental effects of the
species on ecosystem its eradication programme was started by the Silviculturist Uttarakhand.
Cut Rootstock method was adopted which is developed by CEMDE, Delhi University for the
eradication. The objective of the present study is to assess the regeneration and diversity in
different forest stands after the eradication of L. camara. Four eradicated stands in Garhwal
(Pauri district) and four stands in Kumaun (Nainital district) have been selected for the
detailed inventory. The dominated trees in the Garhwal stands were Dalbergia sissoo and
Shorea robusta whereas Pinus roxburghii and Quercus leucotrichophora were the dominated
species in Kumaun stands. Biodiversity and regeneration was assessed by placing quadrats of
1×1m, systematically after every 50 m in rows and columns in all the selected stands. A total
134 species of grasses, herbs, shrubs and trees were reported regenerating in Kumaun stands
of which 15 trees, 18 shrubs and 101 herbs. In Garhwal stands 163 species of grasses, herbs,
shrubs and trees were reported of which 26 trees, 8 shrubs of and 129 herbs were
regenerating. Recruit density in Kumaun stands varied from 54.08×104 individual ha-1 to
148.88×104 individual ha-1whereas in Garhwal stands varied from 56.14×104 individual ha-1
to 197.21 ×104 individual ha-1. Majority of the species in all the stands showed contagious
distribution pattern. Shannon-wiener diversity index of Kumaun stands range between 3.04
and 3.38 whereas Garhwal stands between 3.68 and 4.80. On the basis of the study it may be
concluded that L. camara infested stands, after eradication, slowly and steadily restoring by
plant species through regeneration. For the effectiveness of eradication programme, the new
recruits of L. camara must be removed periodically.
Key words: Invasive species, biological pollution, cut rootstock method, density, diversity
39
28. Tree Water Relation and Regeneration Status of Treeline Species in Tungnath Area
of Uttarakhand
Nandan Singh and Ashish Tewari
Department of Forestry and Environmental Science, Kumaun University, Nainital – 263001
The treeline is the edge of the habitat at which trees are capable of growing in the high
altitude, governed by frigid environments or snow cover. The Himalayan treelines are
amongst the highest in the world and hence experience a different environment. Trees
growing at the alpine treeline very seldom undergo severe water stress because of high
precipitation during the vegetative period but stress can reach lethal levels during winter.
Regeneration behavior of tree species is characterized by their population structure which
depends upon the presence of adequate number of seedlings and saplings.The study sites is
located at treeline area in Tungnath region of Uttarakhand between 3400 – 3520 m altitudes.
The major tree species in the area were fir (Abies spectabilis), oak (Quercus semecarpifolia),
birch (Betula utilis),and the shrubsRhododendron campanulatumformed the
boundary.Average pre dawn water potential values acrossthe species ranged from -2.0±0.4
bars to -15.6±0.2 bars in tree and -2.4±0.3 to -28.8 ±0.5 bars in seedlings.Winter temperature
is the most critical situation for treeline species when the tree water potentialcan be extremely
low. Regeneration of all tree species was very poor in the treeline area which can be
attributed to heavy grazing and high water stress. The density of R. Campanulatum which
formed krummholz vegetation were 250 individual ha-1 at the treeline, had relatively good
regeneration. At several locations R. Campanulatum appeared to be moving upwards into the
alpine meadows as seedling could be found upto 30 – 40 m from the parent shrubs line.
Keywords: Treeline, Water Stress, Regeneration, R. campanulatum.
40
29. Assessing Regeneration Potential and Developing Reliable Indicators of Seed
Maturity in Myrica esculenta, Buch-Ham. Ex D. Don, in Kumaun Central Himalayan
Region
Shruti Shah and Ashish Tewari
Department of Forestry & Environmental Science, Kumaun University, Nainital
E-mail: [email protected]
Myrica esculenta, Buch-Ham. Ex D.Don, commonly known, as Kaiphal is a small moderate
sized evergreen dioecious tree in sub-tropical-temperate region of the Himalaya, found across
an altitude between 900-2100masl. This species is well known for its edible fruit and other by
products. The popularity of the species can be judged from the fact that local people of the
region earn over Rs 14.00 lakh/season from selling the fruits of the species. The regeneration
in M. esculenta is poor in natural habitats mainly due to intense biotic pressure on it. In case
of M. esculenta availability of mature seeds is a problem as all accessible locations are
searched by locals for fruits. The study was carried out in 9 sites varying from lower, mid and
high range of species distribution (900-2100 masl). The seedling density across the sites
varied between 44 and 180 indi/ha. The study indicates that M. esculenta stands maintain
significantly high male tree density and average male female ratio was 5:1. The colour
change from green to dark red/ blackish red appears as one of the useful indicator of fruit/
seed maturity. In addition to the colour change seed maturity and maximum germination
occured in seeds when the weight of 100 fruits was between 41 and 60g, fruit moisture
content between 62 and 69% and seed moisture content between 30 and 32%. Seeds treated
with different concentrations and durations of sulfuric acid (H2SO4) failed to germinate
whereas those treated with hot water gave maximum germination ranging between 33% and
57%. The above parameters can be used as reliable indicators of seed maturity before
undertaking major afforestation/ reforestation programmes in the Indian Himalayan Region
(IHR) to avoid failures.
Keywords: Maturity Indices, Germination, Regeneration, Biotic Pressure
41
30. Developing Nursery Techniques for Propagation of Prunus cerasoides D.Don.
Bhawna Tewari and Ashish Tewari
Department of Forestry & Environmental Science, Kumaun University, Nainital
E-mail: [email protected]
Prunus cerasoides D.Don is a multipurpose tree species which belong to the family Rosaceae
and generally occurs between 1200 – 2400 m altitudes. The regeneration and seedling
establishment of this species was very poor in its natural habitat and varied from 24 – 116
indi/ha. The present study deals with assessing the exact seed maturity time, conditions for
germination and seedling growth. For the study three sites (1740-1800 m) at high elevation
and three sites (1360-1400 m) at low elevation were selected. The seed maturation time was
mid March to mid April. Fruit colour changed from green to red towards maturity. The
moisture content of seed varied between 29.25 ± 2.45% and 31.47± 0.42% across all the sites.
Germination ranged between 55± 1.67% and 63.33± 6.66% across the sites. The moisture
content and germination varied significantly across sites and dates of collection (P<0.01).
There was significantly higher germination in 25˚C temperature when seed was mechanically
treated. The seedlings of all sites were planted out at two leaf stage in different potting
mixtures. The best development of seedling was in the potting mixture soil: sand (2:1) ratio.
The growth (height and collar diameter) varied significantly (P<0.01) across site and growth
media.
Keywords: Seed maturity, Germination, Potting mixture, Seedling establishment
42
31. Antioxidant and Phytochemical Analysis of Two Medicinal Plants of Kumaun
Himalaya
Pritee Pant*and Kapil Khulbe
Department of Botany, D.S.B. Campus, Kumaun University Nainital-263002 Uttarakhand
Email: [email protected]
The Kumaun Himalayas have a great wealth of medicinal plants and traditional medicinal
knowledge. Medicinal plants have long been the subject of human curiosity and need. They
are rich in a wide variety of secondary metabolites and exhibit antioxidant activities.
Micromeria biflora and Zehneria indica, belonging to family labiatae and cucurbetaceae
respectively, as used in colic, fever and many other skin diseases in traditional medicine
system. The present study was aimed to evaluate antioxidant potential and phytochemical
analysis of Micromeria biflora and Zehneria indica in two solvents. Qualitative analysis of
phytochemical constituents was performed following standard method using methanol and
hexane as extractants. Total phenolics, and flavonoids content were analyzed quantitatively
by FCR and AlCl3 method respectively. The radical scavenging activities of the plant
extracts were also determined by 2, 2 – Diphenyl -1-dsicrylhydrazyl radical (DPPH) assay.
The results of phytochemical screening showed the presence of glycoside, phenol, flavonoid,
terpinoid, volatile oil, and alkoloid in both the extract of M. biflora. In this methanolic extract
also showed the presence of protein, tannin and quinon but found absent in hexane fraction.
Z.indica revealed the presence of alkaloid, phenol, flavonoid, glycoside, volatile oil, and
terpenoid in both the solvents, while protein, tannin, and quinon were absent in hexane
extract of this plant. Total phenolic content of M.biflora was found higher in methanol extract
(635.55 mg GAE/ g dry extract) than hexane extract (166.6mg GAE/ g dry extract)and for
Z.indica the value was also recorded high in methanol extract ( 420mg GAE/g dry extract)
than in hexane extract(148mgGAE / g dry extract).
Total flavonoid content for Z.indica was also recorded higher in methanol extract (440 mg
QE/g dry extract) than hexane extract (360 mg QE/ g dry extract) but for M. biflora hexane
extract showed a very high value (737.7 mg QE/g dry extract) as compared to methanol
extract (158.8 mg QE/g dry extract).
In the antioxidant analysis (DPPH assay) methanol and henxane extracts showed a
concentration dependant activity. At the highest concentration used (100µg/ml) the methanol
extract of M. biflora and Z. indica showed maximum free radical scavenging activity
(92.87% and 88.05%) respectively. Hexane extract of both plants presented similar trends
showing 32.01% and 31.16% inhibition respectively. These results are interesting as
methanol extract showed activity closer to that of the standard used (ascorbic acid) which
showed 98.17%activity at 100µg/ml.
The findings indicated promising antioxidant activity of crude extracts of the above Plants
and needs further exploration for their effective use in modern system of medicines.
Keywords: Antioxidant, phytochemicals, medicinal plants, extract
43
32. Diversity Analysis in Pinus roxburghii Sarg. and Mixed Broadleaf Forest of Central
Hiimalaya, India
Neeta Arya and Jeet Ram
Department of Forestry and Environmental Science Kumaun University, Nainital
Email: [email protected]
Forest diversity is the main source of livelihood of the people living in the Uttarakhand
Himalaya. Forest biodiversity is used for fodder, fuel wood, timber, leaf litter for manuring
crop fields, industrial raw materials and several non-timber forest products. Pinus roxburghii
and mixed broadleaf forest are the major forest forming tree species in this region. The study
area is located between 290 20’ and 290 30’ N latitude and 790 23’ and 790 42’ E longitude
between 1350-2000 m elevations in Uttarakhand Himalaya. The forest were thoroughly
surveyed and identified as Pinus roxburghii, Sarg dominated forest and mixed broadleaf
forest. The greater numbers of tree species were present in mixed broadleaf forest in contrast
to this shrub and herb species were greater in pine dominated forest. Pinus roxburghii Sarg,
forest has low tree and herb density while shrub density was low in mixed broadleaf forest. In
tree, shrub and herb diversity were high in mixed broadleaf forest compared to Pinus
roxburghii forest. These forests are exploited variously for different resources and
consequently the vegetation cover is decreasing. In response to these disturbances the species
richness and diversity are changing, which influence the soil and environmental conditions.
Keywords: Forest, Diversity, Richness
44
33. Solid Waste Management through Vermicomposting and Earthworm Gut
Cellulolytic Bacteria
Neha Rajwar and Satpal Singh Bisht
Department of Zoology, Kumaun University, Nainital-263002, Uttarakhand, India
Email: [email protected]
Earthworms, as they are known as soil engineers are helpful in the reconstruction of the soil.
Due to their selective feeding activities they affect the soil health and their physical
properties, therefore it was globally accepted that soil fertility is of prime importance to
ensure food security. Year 2015 was declared as year of soil to sensitize and aware farmers
and soil ecologists to work on sustainable soil agricultural practices. The gut of earthworm is
known as the unique repository of different bacterial communities. The most important
bacterial community which draws the attention is the cellulolytic bacterial community as they
are beneficial for the decomposition of cellulose leading to various practices of soild waste
management and vermicompostig. Cellulose degradation in nature is a slow process but when
the same process is done by the earthworms with the help of their cellulolytic bacteria, is fast
and beneficial. Vermicomposting and composting is a process that gives the same result but
in different manner, cause here the composting material passes through the earthworm gut
which is loaded with the bacteria helpful in quick biodegradation. It is the integrated
technique of composting. The tropical soils are mainly plant nutrient deficient whereas the
majority of nutrients are entrapped in the kitchen wastes and agricultural by products.
Microorganisms and earthworms are the key source for the nutrient flow from one system to
another. When the same matter is treated with the cellulolytic bacteria along with the
beneficiary earthworm it can provide the vermicompost at its best in turn could be utilised as
an effective tool for various agricultural and agronomic practices.
Keywords: Earthworms, vermicomposting , cellulolytic bacteria, soil fertility
45
34. A Comparison of Canopy Cover Measured Through Transect and Densiometer
Methods in Oak Forest of Central Himalaya India
Poonam Prasad and Jeet Ram
Department of Forestry & Environmental Science, Kumaun University, Nainital
Email: [email protected]
The present study deals with the canopy cover of trees in Oak (Quercus leucotrichophora A.
Camus) forest. The study area is located between 29º36’ latitude and 79º48’ longitude
between 1800 to 1900m elevation in Uttarakhand Himalaya. The vegetation cover is
measured in different means. We use to measure canopy cover in two ways as conventional
transect method and densiometer method (an instrument). In Quercus leucotrichophora A.
Camus forest a total of 90 transect and 90 densiometer reading were taken for analysis. The
transect and densiometer reading were taken across the canopy cover as open, moderate and
close. The data were analyzed by applying t-test. The result showed that across the methods,
the canopy cover is significantly different (t = 1.97, P < 0.01). When it compared in different
canopies, it is significantly different in open (t = 2.00, P < 0.04) and moderate (t = 2.00, P <
0.03) while the canopy cover was not significantly different in close canopied forest. To find
out the most appropriate method, the data need to be analysed in details.
Keywords: Canopy cover, oak, transect, t-test
46
35. Zirconium Doped Iron Oxide Nanocomposite for the Adsorption of Cd(II) :
an Eco-Friendly Protocol
Rajesh Kumar, Shah Raj Ali and Mahesh Chandra Arya
Department of Chemistry, D.S.B. Campus, Kumaun University, Nainital 263 002, India
Email: [email protected]
Zirconium doped iron oxide nanocomposite was prepared by precipitation method and their
adsorption potential towards Cd(II) ions has been studied. The synthesized materials was
characterized by energy-dispersive X-ray, scanning electron microscopy and X-ray
diffraction. It was used as adsorbent for the adsorption of Cd(II) ions from aqueous solutions.
Batch adsorption experiments were conducted to investigate the adsorption of Cd(II) ions on
the prepared adsorbent. For adsorption process, A fixed amound of synthesized
nanocomposites was added to a definite volume of Cd(II) ions under optimized physico-
chemical parameters such as adsorption time, concentration of Cd(II) ions, amount of
adsorbent, temperature and pH. The concentration of Cd(II) ions in solution after adsorption
was determined using atomic absorption spectroscopy. The adsorption data followed
Langmuirian adsorption trends. The synthesized adsorbent exhibits high adsorption capacity
and it shows nearly 90% adsorption of Cd(II) ions under optimum condition. The method
used herein for the synthesis of zirconium doped iron oxide nanocomposite is eco-friendly
because the dissolved material present in the filtered solution was the recoverable sodium
chloride, and that can be disposed of safely.
Keywords: Adsorption, zirconium doped iron oxide nanocomposite, Cd(II) ions,
Langmuirian adsorption trends.
47
36. A Comparative Study on Water Quality of River Saryu for Drinking Purposed In
Bageshwer District
Jeewan Gahtori, S. P. S. Mehta, Garima Punetha and Latika Negi
Department of Chemistry, D. S. B. Campus, K. U. Nainital
Email: [email protected]
The river Saryu in Uttarakhand is a majaor source of water. The river in hill area is polluted
up to a large extent due to influent, untreated sewage and domestic garbage. A systematic
study has been carried out to access the quality of water in Saryu river of Bageshwer
(Uttarakhand). In this study different water samples from five major sampling stations were
collected and the samples were analyzed for the Physico-Chemical analysis like pH, Electric
Conductivity, Dissolve Oxygen ,Biological Oxygen Demand, Chemical Oxygen Demand,
Total Hardness, Turbidity etc. The samples were analyzed in different seasons. The particular
data of various parameters indicate that some of the parameters like pH, Electric
Conductivity, Total Hardness, Turbidity are found to be in excess than the permissible limit
which clearly indicates the pollution with seasonal changes. The analysis reveals that the
river water of Saryu in the areas of Bageshwar city needs treatment before being used and it
also needs to be protected from the domestic contamination. The drinking water quality
slightly differs in summer and winter seasons than post monsoon seasons.
Keywords: Water pollution, seasonal variation, saryu river
48
37. Density Relationship for Adsorption Capacity of Liriodendron Tulipifera (Hard
Wood) and Cedrus deodara (Soft Wood)
Anita Rana, Mahesh Arya, Vinay Deep Punetha and Ganga Bisht
Department of Chemistry, Kumaun University, Nainital-263002, India
Email: [email protected]
The adsorption of water by plants is essential for various metabolic activities. Adsorption
process is used for water waste treatment. The extent of adsorption varies in soft wood and
hard wood. In this report, it has been investigated the density relationship of Liriodendron
tulipifera, hard wood and Cedrusdeodara, soft wood and adsorption capacity of its charcoal.
The collected plants hard /soft woods sawdust first heated in air tight jar for 20-30 minutes
until the charcoal is formed. This charcoal was finely powdered in a grinding machine and
then weighed in an electrical balance. In this experiment the charcoal of soft wood and hard
wood used because both having the good adsorption capacity. The density measurement was
performed by xylometric method. On the basis of experimental data, the density of soft wood
(Liriodendron tulipifesra) was 1.6105 g/cm3 with the extent of adsorption x/m 1.8×10-4 mol
and the density of hard wood (Cedrusdeodara) was 2.8470 g/cm3 with the extent of
adsorption x/m 1.3×10-4 mol. Soft wood having lower density shows higher adsorption
capacity and hard wood having higher density shows lower adsorption capacity. The
adsorption capacity of soft wood charcoal is more than the adsorption capacity of hard wood
charcoal because of its small particle size and increasing surface area. Thus this correlation
shows that the adsorption capacity of soft wood i.e. Cedrusdeodarais more than the
adsorption capacity of hard wood i.e. Liriodendron tulipifera.
Keywords: Adsorption, hard wood, soft wood, xylometric method
49
38. Quantitative Estimation of Total Phenolic Content, Total Flavonoid Content and
Free Radical Scavenging Potential of Himalayan Fir (Abies Pindrow (Royle Ex D.Don)
Royle) : an Ethnomedicinally Important Plant
Urvashi Verma1, Kapil Khulbe1 and Tapan Kumar Nailwal2
1Department of Botany, D. S. B. Campus, Kumaun University, Nainital (Uttarakhand) – 263002,
India 2Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital (Uttarakhand) -
263136, India
Email: [email protected]
Reactive oxygen species (ROS) are a class of highly reactive molecules derived from the
various metabolic activities, ROS including superoxide radical, hydroxyl radicals and
hydrogen peroxide molecules are often generated as by product of biological reactions or
sometimes from exogenous factors. There are various evidences suggest that the compounds
especially from natural sources like secondary metabolites from plants have antioxidant
potential have ability to protect the cell by scavenge free radicals.
In this concern a well known ethno-medicinal Himalayan gymnosperm Abies pindrow
(Royle ex D.Don) Royle was selected to evaluate its total phenolic content (TPC), total
flavonoid content (TFC) and free radical scavenging activity by using four different solvents
(hexane, chloroform, ethanol and aqueous). Radical attenuating abilities of four plant extracts
were ascertained by 2, 2-diphenyl-1-picryl-hydrazyl (DPPH), 2, 2̍ [azinobis (3 ethyl
benothiazoline- 6 sulphonic acid) di-ammonium salt] (ABTS) assays.
The ethanol extract observed containing maximum amount of total phenolic content and total
flavonoid content i.e. 488.10±6.30mg GAE/ gm dry extract weight and 255.17±5.27 mg QE/
gm dry extract weight and minimum in hexane extract i.e. 204.76±4.29 mg GAE/ gm dry
extract weight and 106.90±7.18 mg QE/ gm dry extract weight respectively.
The DPPH and ABTS free radical scavenging potential was found highest in the ethanol
extract and lowest in hexane extract ranging 75 to 30 % inhibition with significant IC50
values. The present data explains the multiple free radical scavenging capacity and
antioxidant capacity of A. pindrow leaves. The free radical scavenging activity of the plant
extracts were compared with standards (Ascorbic acid, BHA and BHT). The present study
provides evidence that extracts of A. pindrow leaves are a potential source of natural
antioxidants and could serve as a base for future drugs.
Keywords : Abies pindrow, phenolic and flavonoid content, radical scavenging activity
50
39. Economic Upliftment of Local Communities through Cheura (Diploknema butyracea
Roxb.) Plantations in Kumaun
Krishna Kumar Tamta and Ashish Tewari
Department of Forestry and Environmental Science, D. S. B. Campus, Kumaun University, Nainital
Email: [email protected]
Cheura (Diploknema butyracea Roxb.) is a large tree belongs to the family sapotaceae occurs
in the sub Himalayan tract between 300-1500m from sea level.The species is a principal
source of edible oil in the remote areas of Nepal and Kumaun region of Uttarakhand. It
commonly flowers during cold season and fruit ripens in June-July.The most important part
of the tree is the seed whose kernels contain upto 65% oil/fat and is locally known as “Cheura
Ghee” or “Phulwara Butter”. The present study was carried out at 6 areas located in Nainital,
Champawat and Pithoragarh district during the year 2015-2016. The sites were located
between 950 and 1246m altitude. The purpose of the study was to assess resource base and to
evaluate the oil content of the species. 10 permanent plots of 10×10 m2 were placed randomly
in the study area. The circumference was measured with help of meter tape and height was
measured with the help of Ravi Multimeter. The continuous extraction process was used for
oil extraction using a soxlet apparatus and hexane which was used as a solvent.The density of
the species ranged between 70-240 individuals ha-1. At several areas absence of seedlings and
saplings was conspicuous and the maximum sapling density was 12-16 saplings ha-1. The
flowering occurred between the month of December-Feb and fruits ripened between May-
July. June was the peak month to harvest seeds for oil extraction. The average fruit yield tree-
1varied between 185.21 and 242.12 kg tree-1 the seed yield 32 - 43 kg tree-1 and kernel yield
23.68 – 31.82 kg tree-1. The fruits size ranged from 308.1 ± 1.08 - 1124.6 ± 2.14 mm2 and
seed size from 155.22 ± 0.24 - 268.94 ± 1.16 mm2, fruit colour was yellowish – green and
elongated-oval shaped and seed colour was light brown. The kernel oil yield percentage of
the studied areas ranged from 47.30 ± 1.25 % to 64.76 ± 1.59%. The mean yield of oil tree-1
ranged from 3.6 to 6.0 kg. The market rate of Cheura Oil/Ghee is 100-130 Rs./Kg. The
results indicate that Cheura seeds have good oil content percentage. It can be a suitable option
for income generation of the local community and may play an important role in self-
sufficiency of vegetable oils production in near future.
Keywords: Cheura, oil content, fruits, kernel, kumaun
51
40. Biomass Status In Ungrazed And Grazed Oak Forest Grazingland In The Himalaya,
India
Kamini Tamta and Jeet Ram
Department of Forestry and Environment Science, Kumaun University, Nainital
Forest grazing land is one of the characteristics feature of Himalayan forest. These grazing
land are utilized variously by the human population. The livestock population has increased
during the recent past and the problem of grazing has acquired serious dimensions, as a result
the vegetative cover is decreasing which has resulted in the loss of top soil due to excessive
erosion. Overgrazing may eventually lead to the loss of vegetative cover. Many natural types
of grassland have been destroyed by cultivation or grazing modified by grazing livestock.
Due to an increase in human and livestock populations and continuous overgrazing, besides
cutting of woody vegetation, community grazing lands have deteriorated adversely. In the
present study the dominant tree species is oak and various disturbances in the forest is
creating the forest grazingland. The present forest include 38 herbs and 22 shrub species .The
herbaceous live shoot biomass varied from479.0 ± 5.50 to1945.0±3.45 kgha-1 and 110.0±2.9
to 1762±3.2 kg ha-1 in ungrazed and grazed site respectively. Total Aboveground primary
biomass was higher on ungrazed forest site (5670.6 kg ha-1) compare to grazed site
(3467.0kgha-1).
Keywords: Livestock grazing, disturbance, biomass, oak forest
52
41. Ethnobotanical Studies of Betalghat Region, Along with Kosi Catchment Area,
Kumaun Himalya
Naveen Chandra Pandey1, G C Joshi1 and Lalit M. Tewari2
1 Regional Ayurvedic Research Institute, (RARI) CCRAS, Ranikhet, Uttarakhand,India
2 Departments of Botany, DSB Campus, Nainital-263002(Uttarakhand) India
E-mail: [email protected]
The magnificent Himalaya is well recognized for its bio-physical diversity and socio-cultural
heritage, traditional systems and an ample quantity of indigenous knowledge. Uttarakhand is
a part of Indian Himalayan Region, has a vast number of rivers, tributaries and lakes. Kosi is
an important river of Kumaun Himalaya. It flows in the central part of Almora District and
western part of Nainital District. Betalghat is a block of Nainital district located at the bank of
river Kosi. The study was conducted with the help of Participatory Rural Appraisal (PRA)
technique to document the ethno botanical studies of plants of Betalghat region, along with
Kosi Catchment area. Total 108 ethno-botanical plants species belonging to 56 families, 99
genera, different habitats such as tress (37%), herbs (30%), shrubs (22%), climbers (11%),
were records. Top ten dominant families were Fabaceae (8 species), Caesalpinaceae (5
species), Euphorbiaceae (5 species), Moraceae (5 species), Solanaceae (5 species),
Amaranthaceae, Anacardiaceae, Apocynaceae (3 species), Asteraceae, Combretaceae (3
species). The majority of plant species were used for medicinal purposes (30%), followed by
fodder (21%), fuel (19%), wild edibles (18%), timber (5%), agriculture implements (3%),
religious (2%) and fibre (2%) which were further classify according to plants parts used such
as: leafs (34%), wood (22%), fruit (12%), root (9%), bark (8%), seed (4%), whole plant (3%),
flower, latex (2%), resin, stem, rhizomes, twig (1%). It was found that 80 medicinal plant
species were used by local people for curing 45 diseases such as diarrhea, dysentery, fever,
cough, arthritis, cuts and wounds, skin diseases, asthma, jaundice etc.
53
42. Cultivated Exotic Medicinal Plants in Tarai Belt of Uttarakhand, India
Sumita Rana
Department of Biological Sciences, College of Basic Sciences & Humanities, G.B.P.U.A.&T.,
Pantnagar-263145, Uttarakhand
Email Id: [email protected]
Cultivated exotic flora is important because of dependency of human beings on it for food,
shelter, medicine ecosystem services, aesthetic enjoyment and cultural identity. Exotic
species can also help an ecosystem by providing a missing link in a failing food chain.
Introductions can also be beneficial by conserving species in cases where they are
endangered or threatened in their natural habitats. Exotic plants may be introduce or arrived
there by human activity either deliberately or accidentally, where they do not naturally occur.
These are consider as non native, non indigenous or simply aliens to a particular area. A
survey of such plants was conducted in Pantnagar area of Uttarakhand State. The present
paper deals with study of 91 exotic cultivated medicinal plants of Tarai belt of Pantnagar
region of Uttarakhand. Medicinal plants belong to a big plant group with a great interest due
to its pharmaceutical, cosmetic and nutritional application. In addition, they are also an
alternative to traditional crop with species in high demand at the current international market.
G. B. Pant University is a unique place in Tarai region of Uttarakhand. Tarai region is
situated near the outer Shivalik hills of the Himalaya. Geographically the land area covered
by the University which lies in between the latitudes N 280 59’ 36” – 290 02’ 34” and
longitude E 790 28’ 33” - 790 31’ 12” with an altitude range of 213 to 238 m above sea level.
The present study deals with comprehensive list of exotic cultivated medicinal plant species
at Pantnagar with background information on family, utility, habit and nativity. The present
work involve exploration of exotic cultivated medicinal plants in the entire area of Pantnagar
in different seasons. This is the first assessment of alien cultivated medicinal flora of the
Pantnagar region. There is a lot of scope of these exotic medicinal plants in herbal medicines.
The different parts of the plants are used to cure several kinds of illnesses. The leaf is
predominantly used, and is followed by roots, tubers, and rhizomes. The indigenous
community prefers these plants as home remedy against fever, skin problems, leucorrhoea,
rheumatism, headache, indigestion, etc.
Key words: Exotic, medicinal plants, cultivated, Pantnagar
54
43. Screening for Phosphate Solubilization Potential of Two Rhizospheric Fungi
Prabha Pant, S. C. Sati and P. Kumar
Department of Botany, D.S.B. Campus, Kumaun University, Nainital-263001,
Uttarakhand, India
Email: [email protected]
Microbes are known as an important natural phosphate solubilizing agent in
rhizospheric soil. Therefore the phosphate solubilization potential of two selected fungi
viz. Aspergillus fumigatus and Fusarium oxysporum isolated from rhizospheres of Acer
oblongum and Cupressus torulosa respectively were screened for the present study.
Phosphate solubilization potential of selected fungi was observed by solubilization
index (SI) using Pikovskaya’s (PKV) agar medium. Both the isolated fungi were found
positive for phosphate solubilization. A. fumigatus was found comparatively more
potent to F. oxysporum for the phosphate solubilization. A higher solubilization index
(SI-2.2) was recorded for the former fungus while for latter fungus SI was recorded up
to 1.3 only. The use of phosphate solubilizing microorganisms as a phosphate bio
fertilizer under varied ecosystems may be beneficial to improve the soil fertility and
crop production.
Keywords: Rhizospheric fungi, phosphate solubilization, A. fumigatus, F. oxysporum.
55
44. Phytochemical analysis and Evaluation of Kumaun Himalayan Litsea umbrosa Nees
Bark Extract for its Antimicrobial and Antioxidant Potentiality
Parikshit Kumar and S. C. Sati
Department of Botany, D. S. B. Campus, Kumaun University, Nainital-263001, India
Email: [email protected]
Medicinal plants are playing an important role in human life since the ancient time. Kumaun
Himalaya possesses a great wealth of medicinal plants. In recent times due to rapid
development of multidrug resistant pathogens and high cost of new generation antibiotics
forced the interest of scientist to look for the antimicrobial substance from natural sources.
Therefore, the present study was undertaken to explore the antioxidant and antimicrobial
activity of Litsea umbrosa Nees (Lauraceae), an angiospermic plant of Kumaun Himalaya.
The bark of plant was collected in the month of April, 2015 from Nainital, Kumaun
Himalaya. Methanol extract of bark was tested against pathogenic bacteria and fungi for their
antimicrobial activities. Antioxidant activity of L. umbrosa bark extract was also carried out
by employing standard technique. Detection and quantification of the presence of secondary
metabolites was carried out by gas chromatography coupled with mass spectrometry.
GC-MS analyses of methanol bark extract of L. umbrosa, revealed the presence of sixty six
identified components representing 100% of the total extract composition. 5-
Benzofuranacetic acid, 6-ethenyl-2,4,5,6,7,7A-hexahydro-3,6-dimethyl-ᾳ-methylene-2-oxo,
methyl ester (19.04%), 4-Methyl-4-[2-(5-methyl-furan-2-yl)-cyclopropyl]-pentan-2-one
(9.78%), 4-Methyl-7,7A,8A,9-tetrahydro-2H-[1] benzoxireno [3,4-h] chromen-2-one
(8.75%), as major components. Antioxidant activity showed higher DPPH scavenging,
moderate hydrogen peroxide scavenging and lower ferric reducing activities of the plant. The
antimicrobial activity of the methanol extract was found most active against all the tested
pathogens and this inhibitory effect indicates its broad spectrum activity against a panel of
microbes and can be exploited for the management of the most common human and plant
bacterial diseases.
Keywords: Litsea umbrosa, GC-MS, antimicrobial activity, antioxidant activity and plant
extracts
56
45. Impact of Winter Water Stress on Seedlings of Quercus Leucotrichophora A.
Campus and Rhododendron Arboreum Wall
Amit Mittal and Ashish Tewari
Department of Forestry and Environmental Science, Kumaun University, Nainital-263001
Tree water potential was monitored over the winter season in Q. leucotrichophora and R.
arboreum seedlings. The rainfall and temperature data were taken from ARIES (Nainital),
two study sites were selected for the study located at Kailakhan 1760 m and Kilbury 2160 m.
The rainfall and temperature data of last five years (2011-2016) when compared with earlier
years data (2005-2010) indicates that winter rainfall has declined and winters have become
milder. The study commenced from the Ist week of December 2014 and continued till
February 2015. The tree density of Q. leucotrichophora and R. arboreum varied between 70
to 250 trees/ha and seedlings 40 and 80 seedling/ha across the sites. The pre-dawn water
potential in seedling of Q. leucotrichophora started to decline from Ist week of December and
values ranged between -1.2 MPa to -1.36MPa. The stress intensified and by mid-January the
seedling had became highly stressed and browning of leaves was visible with pre-dawn water
potentials varying between -2.8 MPa and -3.1 MPa. In R. arboreum seedling in December Ist
week the Pre-dawn water potential values were -1.39 MPa and had decline to -2.6 MPa by
mid-January end. It appears that increasing stress level due to decline in winter rainfall by
over 45% can have a severe impact on regeneration of these two important Himalayan
species.
Keyword: Water Potential, water stress, himalayas, regeneration, seedling
57
46. Ethnobotany and Indigenous Uses of Urtica dioica L. in Uttarakhand Himalaya
Kanchan Upreti and Y. P. S. Pangtey
Department of Botany, D.S. B. campus, Kumaun University, Nainital- 263002, Uttarakhand
Email: [email protected]
Urtica dioica L. locally known as Shisoon, is a herbaceous perennial flowering plant and is
the best –known member of the nettle genus Urtica. Uttarakhand is an ethnic region where
the plant is a part of the local culture. There is historical evidence of use of species being
used in folk medicine, cooking and fibre production. Urtica dioica L. is the main species used
for these purposes. This paper deals with the indigenous uses and ethnobotany of its stem,
leaves, root, fibre and aerial parts. It is concluded that in the light of present commercial and
industrial uses of Urtica dioica L. its cultivation should be promoted in Uttarakhand and
other parts of the Himalayan region of India for folk uses.
Keywords: Ethnobotany, fibre, medicine, Urtica, Uttarakhand.
58
47. Essential Oil Composition and Antifungal Activity of Pimpinella Achilleifolia (DC)
Clark
Kiran Tiwari, Geeta Tewari, Gitu Kunwar, Chitra Pande
Department of Chemistry, D. S. B. Campus, Kumaun University, Nainital, Uttarakhand, India
Email: [email protected]
The need for alternatives to synthetic fungicides has necessitated the search for effective
biodegradable natural products from plants. The genus Pimpinella is agronomically important
as they possess distinctive aromas and flavours and are cultivated for their aromatic seeds
used in medicine and as spice. In the present investigation, essential oil components of
Pimpenella achilleifolia have been identified and mycelium growth inhibition potential of the
oil was evaluated against-three plant pathogenic fungi. Seventeen compounds were identified
representing 97.23% of the total oil. Monoterpene hydrocarbon was the dominating class
comprising terpinolene (36.3%), γ-terpinene (21.5%), β-pinene (13.1%), limonene (7.1%)
and p cymene (6.7%) as major compounds. E-(β)-Farnesene, germacrene D and isodausene
have been observed for the first time in the oil from this plant. γ-Terpinene and terpinolene
have not been reported as major compounds in P. achilleifolia in previous reports. The oil
showed strong inhibitory effect on the mycelial growth of Rhizoctonia solani followed by
Sclerotonia sclerotiorum and Fusarium oxysporum.
Keywords: Pimpinella achilleifolia, essential oil, Apiaceae, terpinolene, γ-terpinene,
antifungal activity
59
48. Nutraceutical Plant Diversity in Kumaun Himalaya of Uttarakhand, India
Neelaxi Pandey and Satpal Singh Bisht
Department of Zoology, D.S.B.Campus, Kumaun University Nainital, Uttarakhand, India.
Email: [email protected]
Kumaun Himalayas are mostly covered with forest resources, including still untrapped flora.
The flora present within this hot spot of biodiversity rich area is utilized as food, fodder, fuel,
timber, agricultural, medicine and many other folk practices by the local inhabitants. Among
these, wild edible plants play an important role in food supplement during scarcity for local
inhabitants, which have great nutraceutical value. The information is based on traditional
knowledgesystem (T.K.S), Vedic and Ayurvedic literature available. During the study it was
observed that Myrica esculenta (kafal), Rubus ellipticus(hisalu),Pyracantha crenulata
(ghighharu),Ficus auriculata (timul) are the commonly utilized as wild edible fruits.
Conserving wild fruit can be a good source of livelihood for the local community. It can be
encouraging step toward conserving species of wild plant which are endemic to Kumaun
region of Uttarakhand for nutritional and therapeutic applications.
Keywords: Kumaun Himalaya, livelihood, nutraceutical, wild edible fruit, traditional
knowledge system
60
49. Biotechnological Traditional Knowledge and Related IPR Issues
Pankaj Kumar1, Dr. Ameeta Sharma1, Dr. Shalabh Gupta 2 and Dr. A.K. Paliwal2
1The IIS University, Jaipur-302020
2S. B. S. Govt. P. G. College, Rudrapur, Uttarakhand
Email: [email protected]
In view of numerous biotechnological inventions based on biological traditional knowledge
in India and the applications for patent filed for some of them. Although an invention to get
patentable, needs to qualify certain criteria including novelty and inventiveness. Some
traditional knowledge sometimes may not be found codified and so such inventions
sometimes escape during prior-art search establishing patentability particularly novelty and
inventiveness. Some of the earliest such examples are neems, haldi, basmati etc. Many times
such granted patents were objected and revoked after establishing its availability in public
domain being a part of traditional knowledge at any of the corner of the world. In view of
huge cost and time involved revoking such granted patents, India have been developed a
TKDL which has been made accessible to patent examiners authorize for international patent
search.
Another related issue in the subject is bio-piracy as hardly any benefit sharing out of
commercialization of such invention based on such biodiversity and the traditional
knowledge. This work intended to analyze the number of such patent application accepted
and rejected, study impact of TKDL rejecting of patent applications along with some case
study, analyzing the particular ground of the rejecting such applications for patent, arranging
some case studies of successful commercialization of such patents, study the benefit sharing
with the traditional society out of commercialization of inventions based on their traditional
knowledge.
Keywords: Benefit sharing, biodiversity, biological traditional knowledge, bio-piracy,
patentability, public domain, TKDL
61
50. Litter Decomposition in a 18-Year Old Holoptelea Intrigrifolia Planch Plantation in
A Sub-Tropical Forest of Tehri Garhwal
Pramod Kumar and R.B. Kuniyal
Department of Botany, HNBGU Campus Badshahithaul, Tehri
The present study was conducted in an 18 year old Holoptelea integrifolia plantation in a
foot-hill forest near Dhalwala village of Tehri District of Garhwal Himalaya. The period litter
after 12 months of decomposition amounted 2.27% for this species. The period from May to
November exhibited rapid loss in litter weight. The removal of litter weight was significantly
related with time elapsed. The value of decomposition constants (K’) was 0.977. The litter
decomposition process which is caused of microbes was maximum during rainy season due
to favorable conditions of temperature and moisture. The paper discusses details of seasonal
leaf decomposition of this species.
62
51. Exploration of Plant Derived Neutraciticals against Lung Cancer Using
In Silico Tools
Priyanka Maiti1, Mahesha Nand2, Hema Joshi1 and Subhash Chandra1
1.Department of Botany, Kumaun University, S.S.J Campus, Almora, Uttarakhand, India.
2.Department of Biotechnology, Kumaun University, Bhimtal Campus, Bhimtal, Uttarakhand, India.
Email: [email protected]
Nutraceuticals have established their potential roles in the protection of human health against
various diseases. The present investigation aims to explore some novel plant derived
neutraciticalsagainst lung cancer by using in silicotools. In this context a library of 150
phytochemicalswere screened against vital targets involved in lung cancer. Nine
phytochemicals were screened namely Betulin, Betulinic acid acetate and 3-B-acetoxyurs-11-
en-13-B from the plant Rhododendron arboreum Sm. (Ericaceae), 4-(-L-
rhamnopyranosyloxy) benzyl isothiocyanate from Moringa oleifera Lam. (Moringaceae),
Germacrene-B and 2''-(S)-α-methylbutyrylalkannin from Artemisia indica Wild (Asteraceae),
Murrayanol, Girinimbine and Mahanimbine from Murraya koenigii (L.) Spreng.L.
(Rutaceae). All the screened moleculesshowed efficient binding capacity with thirteen
different target proteins in terms of H bonding that were evaluated via Lig plot interaction of
the protein ligand complex. Druglikeness and toxicity profile of the screened molecules were
checked by using FAF-Drug3 and admetSAR server where all the hits showed acceptable
results. Therefore findings of the present study suggest that these hit molecules may be used
as neutraciticals for further drug development against lung cancer.
Keywords: Virtual screening, druglikeness, lig plot, admetSAR, lung cancer, phytochemicals
63
52. Ecotoxic Investigations on Agro Ecosystems with Special Reference to
Earthworms
Rashi Miglani and S. P. S. Bisht
Department of Zoology, D.S.B Campus, Kumaun University, Nainital-263001
Email: [email protected]
Earthworms maintain the vitality of soil and provide key functions that favour many positive
ecosystem services such as nutrient value of soil by converting biodegradable material and
organic waste into rich vermicast and with these properties it also enhances decomposition,
formation of humus, development of soil structure physical fragmentation, soil aeration ,
chemical degradation, transformation and translocation of organic matter therefore they are
considered as an important soil organism in development and maintenance of soil fertility
providing sustainability to agro ecosystems. These services are important for sustainability of
agro ecosystems but are degraded by intensive and irrational use of practices such as use of
pesticides which often result in loss of biodiversity. Earthworms represent a large fraction of
soil living biomass in many temperate ecosystems and play an important role in soil
functioning and also increase soil structure via burrows or casts and enhance soil fertility
through partial digestion and communities of soil organic matter. Therefore they are
considered as one of the most suitable animal for testing the toxicity of chemicals in soils and
have been adopted as standard organism for eco toxicological testing. Acute and chronic
toxicity test have been used to assess the toxicity of contaminants, with changes in mortality,
biomass, reproduction rates and behavioral response representing the endpoints of the toxicity
testing in various soil ecosystems.
Keywords: Earthworms, eco toxicity, pesticides, agro-ecosystem, ecosystem services
64
53. Epiphytic Orchids and their Host Species in Baliyanala Catchment Area of Nainital,
Kumaun Himalaya
Renu Suyal1, Jeewan S. Jalal2 and Lalit M. Tewari3
1GBPNIHESD, Kosi, Kataral, Almora, 2B. S. I. Pune, 3Botany Department, D. S. B. Campus,
Kumuan University, Nainital
Nainital catchments lies between 29° 19′-29° 28′ N latitudes and 79° 22′-79° 38′ E longitudes
in the Kumaon Himalaya. The entire area is hilly and covers approximately 208.5 sq kms and
has an altitudinal range from 500 to 2600m asl. To document the epiphytic orchids and their
host species Balianala catchment (300 – 1600 m) area was surveyed. A total 22 epiphytic
orchids were recorded in the Baliyanala catchment area of Nainital region having 19 host
species. Quercus leucotrichophora (6) and Sapium insigne (5) supported maximum number
of orchid species followed by Syzygium operculatum, Bombax ceiba and Pyrus pashia (4).
The occurrence of Coelogyne cristrata and Pholidata articulata on both the angiospermic
host as well as gymnospermic host indicates their wide range of adaptability.
65
54. Impact of Forest Fires on Stand Structure and Regeneration
Ripu Daman Singh
Department of Forestry and Environmental Sciences, D.S.B. Campus, Kumaun University, Nainital
Email: [email protected]
Fire is a widespread process in earth system and plays a key role in ecosystem composition
and distribution. Ecologists, bio-geographers and paleo-botanist have long ignored fire as a
key ecosystem process; its importance is now being revealed more than ever by attempts to
model global vegetation change. Recurrent forest fires decreases the green cover through
prevention of regeneration and leads to the slow death of the forests. While the relative
importance of fire in modifying the global climate is increasing our understanding of how
forest fires might affect regional climate (micro climate) is still rather limited, probably
because of lack of systematic monitoring of the fire cases. So far enough studies have not
been carried out on the impact of forest fires on the vegetation of the Central Himalaya. In
context to soil, low to moderate severity fires; promote renovation of the dominant
vegetation through elimination of undesired species and transient increase of pH and
available nutrients. Fires also have several negative effects on soil, as they cause significant
removal of organic matter, deterioration of both structure and porosity, considerable losses
of nutrients through volatilisation, ash entrapment in smoke columns, leaching and erosion,
and marked alteration of both quantity and specific composition of microbial communities.
This work is a review of the up-to-date literature dealing with changes imposed by fires on
stand structure and regeneration and properties of forest soils. Ecological implications of
these changes are described.
Keywords: Central Himalaya, forest fire, soil properties, tree structure and regeneration
66
55. Radiation Absorber Plants
Shalini Rawat
Government P.G. College, New Tehri, Tehri Garhwal
Email: [email protected]
Radioactive substances from nuclear program me structures are one of the major toxicant
causing serious health hazard. These manmade radiations include X-ray machines radioactive
fall-outs, nuclear reactor waste, TV, computers etc. Effect of radiation may be somatic and
genetic. Most genetic effects are brought by manmade radiations.
Plants on one hand using the electromagnetic radiation from sun for one of the most
important vital activity of earth called Photosynthesis and on the other hand protecting us
from harmful radiations. There are however, many natural compounds with radio-protective
activity. Such compounds include sulfhydryl-containing compounds and anti-oxidant
nutrients such as vitamins C and E, beta-carotene, N-acetyl cysteine and selenium, along with
a range of phytochemicals found in plants such as Ginkgo biloba, Vitis vinifera (Grape),
Ocimum sanctum (Tulsi or holy basil). Some pants have capacity to absorb harmful radiation
of computers like Aloe, Cactus, etc. Such study can be helpful in minimizing radiation
pollution. Present review paper emphasizing Botanical, Ecological and Economic aspects of
some plants.
67
56. Fire Adaptations in Some Important Tree Species
Surabhi Gumber1 and Jeet Ram2
1Department of Forestry and Environmental Sciences,
2Department of Forestry and Environmental Sciences, D. S. B. Campus, Kumaun University,
Nainital
Email: [email protected]
Fire is a widespread phenomenon and recurrent disturbance factor on our planet since the
origin of terrestrial plant. Plant attributes such as bark thickness, serotiny (late to open),
branch shedding, resprouting capacity and germination by heat and smoke are widely
recognized as fire-adapted in fire-prone environment. Responses of plants to fire are
dependent on physical properties of fire. Changes in fire regime often by increase or
decrease in fire frequency and intensity can be threatened by plant species attributes.
Literature review reveals that the ecological significance of annual fires on forest formations
is significant. Frequent and uncontrolled fires adversely affect on vegetation and plant
diversity. In tropical forests, it is reported that due to frequent fires tree species exhibit
adaptive traits such as bark thickness, ability to repair fire scars, resprouting capability and
seed adaptations. It has been reported that in Eucalyptus regnans regeneration has been
recorded high at fire affected sites in temperate Australia. In this review, we have gone
through several research works, projects and books, having an aim to identify functional
attributes, which are adapted to fire in several plant species, such as Pinus etc. The literature
compiled in the present review, helps in identifying the fire adaptive species and their
characters for rejuvenation.
Keywords: Adaptations, adaptive attributes, fire regime, vegetation composition
68
57. Study on the Ecological Importance of Aquatic Avian Fauna of Wetlands of
Kumaun Region, India.
Vinay Singh, Aman Verma and Satpal Singh Bisht
Department of Zoology, D. S. B. Campus, Kumaun University, Nainital-263001
Email: [email protected]
The wetlands of Uttarakhand provide shelter to about 175 wetland avian species, out of
which 134 species are water birds and the remaining 41 species are water dependent birds.
Wetlands both natural and artificial, supports a high diversity of resident and migrant water
birds. Water birds act as an important indicator of the health of ecosystem. At the global
level, water birds form an important link in food web and nutrients cycle. In this study, we
especially address the water birds, which play key functional role in many aquatic
ecosystems, as predators, herbivores and vectors of seeds, invertebrates and nutrients.
Although these activities have often been overlooked by researchers. Water birds can
maintain the diversity of other organisms, control pests, act as effective bio-indicator of
ecological conditions and act as sentinels of potential disease outbreak. They also provide
important provisioning (meat, feathers, eggs, etc.) and culture services to society. In this
study, we exclusively focus on water birds and aim to examine how water birds provide the
important services to ecosystems. It has been reported that the population of wetland water
birds has been declined significantly. However, the studies on ecological importance of
wetland water birds in Uttarakhand region are scanty as per available literature.
Keywords: Bio-indicators, herbivores, migrant birds, pest, predators, wetland
69
58. New Horizons in the Field of Lichenology: Case Study from Kumaun Himalaya
Yogesh Joshi
Lichenology Laboratory, Department of Botany, S.S.J. Campus, Kumaun University Almora, 263
601, Uttarakhand, Iindia
A lot of work has been done on floristic diversity of lichens of Kumaun Himalaya and
approximately 700 species have been reported from the study region. In the present study the
author has tried to summarize the work done by him and his team during the last five years
and tried to open up some unexplored aspects in the field of lichenology viz. secondary
fungal diversity associated with lichens (lichenicolous and endolichenic fungi), lichens as
biomonitoring tool to assess the health of Kumaun Himalaya, and a lot more. Though a lot
need to be done in this field, but the preliminary data generated during the present study will
not only act as a baseline data but will also be of immense importance to young botanists,
environmentalists, ecologists.
Keywords: Biomonitoring, climate change, diversity, fungi, india, lichens
70
59. Watershed, West Himalaya, India
Bhaskar Ch. Joshi, R.S. Rawal and K. Chandra Sekar
G.B. Pant National Institute of Himalayan Environment and Sustainable Development,
Kosi-Katarmal, Almora, Uttarakhand
As major food for livestock, fodder plants are considered integral for rural livelihoods. These
plants are mainly harvested from forests, grasslands, and agriculture and agro-forest area. In
Kumaun, West Himalaya, fodder is mainly collected from forests by way of harvesting
vegetative biomass of selected trees, shrubs, climber and herbs. Present study was analyse the
diversity, utilization pattern, energy budget of fodder collection from forest and agro-forest
landscapes at different altitude zones of Hat-Kalika watershed in Gangolihat (Pithoragarh)
Uttarakhand. A total of 57 fodder species trees (29), shrubs (9), climbers/lianas (6) and herbs
(13) were recorded. On an average the collection of leaf fodder (Green) from forest system
ranged 21.7-25.6 kg/hh/day, whereas from agro-forest system it ranged between 3.4-4.5
kg/hh/day. Fodder collection was higher in warmer (March to October) than cooler
(November to February) month. The energy budget for collection varied from 251.04-439.32
MJ household-1 year-1 and total collection days spent in month HH -1 range 7.5-13.1 days. The
present study provides an insight on species under pressure as well as the average time spent
for fodder collection. The data sets along with other energy budgets viz. fuel wood and water
collection would be helpful in designing suitable strategies to reduce drudgeries of rural
populace and promotion of natural resource management in the Watershed.
Keyword: Budget, conservation, diversity, energy, native, resource management, utilization
71
60. Significance of Community Participation in Promoting the Conservation of Musk
Deer and its Preferred Fodder in Uttarakhand, in Uttarakhand Himalaya
Ira Tewari
Department of Forestry and Environmental Science, D. S. B. Campus, Kumaun University, Nainital-
263001 (Uttarakhand)
Email: [email protected]
The mighty Himalaya is blessed upon by Mother Nature as she has bestowed an immense
wealth of natural resources in its cradle. Himalaya is beautiful but environmentally fragile
youngest mountain belt. It is prone to ecological unstability, intense seismicity but is ushered
with tremendous natural resources, both renewable and non-renewable. Himalaya is marked
by a variety and complexity of natural phenomenon like terrain, geology, hydrology, climate,
vegetation, soil, wildlife: the region has its own distinctive aura. Along with several natural
resources, one of the prized assets of the Himalayan region is – the Musk deer, which also
happens to be the state animal of Uttarakhand. Musk deer is a prized asset of the globe and its
population is depleting alarmingly, especially in the Himalayan region. In the Red Data Book
of International Union for Conservation of Nature and Natural Resources, the species is listed
as endangered (IUCN 1974). The 1972 Wildlife Protection Act of India and the Wildlife
Protection Amendment Act of India, 1991, has placed it in the Schedule I of ‘Endangered and
Rare” species. The CITES (Convention on International Trade in Endangered Species of
Fauna and Flora) has listed the Himalayan Musk Deer in Appendix I and prohibited musk
trade in the international level. In the Himalayan region Poaching and habitat destruction are
the main reasons for the sharp decline in the population of this species. To stop the further
depletion of this species, the need of the hour is to conserve it by structuring of confined
farms in appropriate elevations. Community participation is an important aspect in the
conservation of this animal. For the smooth structuring of the confined farms, the availability
of the, presently, depleting fodder is of utmost importance. The musk deer prefers around
fifty varieties of plant species as its fodder. Due to the accelerating rate of thinning of forests
in the Himalayan region, the availability of the fodder species is also diminishing. So, with an
active participation of the community fodder availability can be meted out. Therefore, the
Structuring of these confined farms shall go a long way in conserving this endangered animal
species as well as shall pave way for boosting the eco-tourism and ultimately strengthening
the economy of the state as well.
Keywords: Himalaya, endangered, poaching, community participation, fodder
72
61. Synthesis of Some New Azetidinones Derivatives with Possible Biological Activities
Jagmohan Singh Negi
Department of Chemistry, L. S. M. Govt. P. G. Collage Pithoragarh, Uttarakhand(INDIA)
Email: [email protected]
The present study was conducted to evaluate the antimicrobial activity , many phenols and
compounds with phenolic groups have antifungal potency A large number of fungicides are
formulated as wettable powders; this is the form most commonly used for spray
mixes.Nodern wettable powerders are easily wetted and disperse well in water . In the
present review, emphasis is given on diverse pharmacological properties associated with
substituted thiazolidinones and structurally related thiazolidines. Such a chemical is called a
‘’fungistat” and the phenomenon of temporarily inhibiting the growth is ‘’fungistasis”. Some
other chemicals, like certain phenanthrene derivatives and Bordeaux mixture, may inhibit
spore production without affecting the growth of vegetative fungistate hyphae. They simply
inhibit fungus growth temporarily.If the fungus is freed from such substance, it would revive.
These are called ‘’antisporulaaaaants’’. 2-amino 4-Phenyl Oxazole is condensed with
appropriate aromatic aldehyde in methanol was refluxed on water bath for 1 hr.Various.
obtaining gave 2 Substituted N-[4-Phenyl-2-thiazolyl]-2-imino (4I-hydroxy phenyl)
methylene Which were converted into N-(4-Phenyl thiazolyl ) 3-Chloro-4ll-(4l-hydroxy
phenyl )-2-azetidinones and by reaction with chloroactyl chloride respectively and
synthesized compounds showed moderate to good antifungal activity with respect to standard
drugs
Keywords: 2-Amino 4-phenyl oxazole, EtOH, antimicrobial activity.
73
62. Development of a Reporter System for Screening and Characterization of
Aromatase Inhibiting Potential in the Phytoextracts/Phytochemicals from Uttarakhand
Flora
Ankita H. Tripathi1, Rekha Gehtori1, Ashutosh Paliwal1, Penny Joshi2 and Santosh K.
Upadhyay1
1 Department of Biotechnology, Kumaun University, Bhimtal Campus, Bhimtal
2 Department of Chemistry, D.S.B. Campus, Kumaun University, Nainital
Email: [email protected]
Aromatase is an enzyme of cytochrome P450 group, responsible for catalyzing the conversion of
androgens to estrogen. In human this protein is encoded by CYP19A1 gene and is expressed in
numerous tissues of the body including breast, skin, brain, adipose, muscles and bone tissue.
Significantly higher expression of CYP19A1 in breast cancer than in circulating plasma, suggests
locally increased aromatase expression for estrogen biosynthesis near or within cancerous tissue.
Numerous studies now implicate increased Aromatase activity with cancer progression; however,
only limited studies till now have explored the role of Aromatase in Epithelial-mesenchymal
transition (EMT). EMT is a biological process where well differentiated epithelial cells undergo
various biochemical changes and attain properties of mesenchymal cells those include attaining higher
invasive and migratory potentials. Importantly, in cancer, EMT is known to promote metastasis and
therapeutic resistance as well. In a previous study, our group has identified Small molecule inhibitors
(SMIs) capable of EMT reversal in NSCLC using a 'connectivity map' approach, and in the list of
predicted potent inhibitors/reversing-agents of EMT, a very impressive representation of Aromatase
inhibitors was observed. Therefore, the aromatase inhibiting (AI) property of any
drug/phytochemical/phytoextract may also be suggestive of its EMT-inhibiting potential and was
further validated by certain already published studies. So, owing to the EMT association of Aromatase
and importance of EMT-inhibition in the prevention of metastasis and drug resistance, identification
of novel inhibitors of EMT is crucial.
Uttarakhand is well known for its medicinally important floral diversity. Many plants of this region
are known to have AI-activity, such as Angelica glauca, Oroxyllum, Linum usitatissimum, Nigella
sativa, Trifolium pratense, Camellia sinensis, Aconitum heterophyllum etc., whereas, for few other
(related) plant species, the AI-activity is yet to be identified / characterized. The present study
therefore aims at developing an efficient and cost-effective screening system for identification and
characterization of AI-potential in the phytochemicals/phytoextracts. In the first part of this study we
cloned CYP19A1-promoter in pGL3-basic reporter vector, which on transfection in suitable cancer
cell-line would enable it to reflect the impact of phytoextract on expression of aromatase. Further, to
characterize the Aromatase-inhibition in enzymatic term, we identified the markers specifically
associated with inhibition by mining microarray data of breast cancer cell-line MCF7 exposed to
commercially available inhibitors of Aromatase. This study led to identification of total 24 markers
(12 upregulated and 12 downregulated) in breast cancer transcriptome, which in different subsets
could be used to detect enzymatic inhibition of Aromatase.
The AI-screening system developed in this study would be useful for identification of AIs, those may
help in targeting metastasis and drug resistance in cancers as inhibitors of EMT. The present screen is
unique in terms of mechanistic evaluation of AIs (Inhibition of CYP19A1 expression vs. inhibition of
Aromatase-enzymatic activity), possible with this. Furthermore, unlike previously known radioisotope
based, costly protocol based on direct evaluation of aromatase (enzymatic) activity, the present
method is cost-effective and non-hazardous.
74
63. Genetic Diversity in Black-Seeded Soybean (Glycine max L.) Landraces of
Uttarakhand State
Ranjana Singh1, Rajni Rawat1, P.S. Mehta1, S.K. Verma1, L.M. Tiwari2 and I.S. Bisht1
1ICAR-NBPGR Regional Station, Niglat (Bhowali), Uttarakhand-263132
2 Department of Botany, DSB Campus, Kumaun University, Nainital-263001
Black-seeded soybean (Glycine max L.) or bhat is widely cultivated in Uttarakhand hills. Soybean is
considered to be a source of complete protein that contains significant amount of all the essential
amino acids that must be provided to the human body because of the body’s inability to synthesize
them. It is also an important component of local food culture of Uttarakhand hills and dominates
kitchens in the Garhwal and Kumaon regions of the state with several preparations. The present
communication describes the range and pattern of diversity of about 200 native landraces of soybean
maintained at the ICAR-National Bureau of Plant Genetic Resources, Regional Station, Niglat
(Bhowali), assembled from different agro-ecologies of Uttarakhand hills. The entire landrace diversity
was characterized for a set of 15 morphological traits, 10 quantitative and 5 qualitative. Besides, the
oil and protein analyses were also done for selected representative accessions. Wide range of
variations for different traits was recorded. Promising accessions were identified for different traits to
facilitate use of conserved germplasm in crop improvement and other biological researches.
Keywords: Soybean (Glycine max L.), native landraces, genetic diversity, germplasm
characterization
75
64. Taxononomic and Ecological Studies on Catamixis baccharoides Thomson Endemic
Species of Uttarakhand in Dehra Dun & Adjacent Siwaliks
Pooja Chauhan and S. K. Agarwal
D. B. S. (P.G.) College, Dehra Dun 248001
The paper deals with the distribution and ecological status of the endemic plant Catamixis
baccharoides Thoms. This plant is belongates family Asteraceae is reported from foothills of
Dehra Dun and adjacent siwaliks Uttarakhand.
Keywordes: Catamixis baccharoides Thomes., Dehra Dun, Uttarakhand, endemic
76
65. Lichens: A Promising Source of Nutraceuticals and Pharmaceuticals
Vartika Pant and P.B. Rao
Department of Biological Sciences, College of Basic Sciences & Humanities
G.B. Pant Univ. of Agric. &Technology, Pantnagar, US Nagar
Email: [email protected]
Lichens represent a unique division in the plant kingdom. They have been used in Traditional
systems of medicine including Traditional Indian Medicine (TIM), Traditional Chinese
Medicine (TCM), Homeopathic and Western Medical Herbals. Lichens have been using in
the treatment of diverse diseases like arthritis, alopecia, constipation, kidney diseases,
leprosy, pharyngitis, rabies, infection, worm and infestation as reported in traditional systems
of medicine. The medicinal utility of lichens is due to the presence of secondary metabolites
such asusnic acid,atranorin and etc. Lichens are an untapped source of biological activities of
industrial importance and their potential is yet to be fully explored and utilized. Lichen-
derived bioactive compounds hold great promise for biopharmaceutical applications as
antimicrobial, antioxidant and cytotoxic agents and in the development of new formulations
or technologies for the benefit of human life. The present study is made on investigation of
different antioxidants, mineral elements and bioactive compounds in different species of
lichens collected from Kumaun region of West Himalaya.
Keywords: Lichens, antioxidants, secondary metabolites, Kumaun Himalaya
77
66. Age of Rhizome Segments Determine Ramet Regeneration and Growth of
Hedychium spicatum Sm.
S. S. Koranga1, 2, 3, K. S. Negi2, L.M. Tiwari3, A.K. Negi4, M.M. Pandey5, S. Srivastava5 and
A.K.S. Rawat5
1National Medicinal Plants Board (NMPB), Ministry of AYUSH, Govt. of India, New Delhi, India
2ICAR- National Bureau of Plant Genetic Resources, R/S Bhowali, Nainital, Uttarakhand, India 3Kumaun University Nainital, Dept. of Botany, Nainital, Uttarakhand, India 4HNB Garhwal University, Dept. of Forestry, Srinagar, Uttarakhand, India
5CSIR-National Botanical Research Institute, Dept. of Pharmacognosy&Ethnopharmacology,
Lucknow, India
Hedychium spicatum (Zingiberaceae) is a perennial rhizomatous herb and its rhizomes are
source of essential oil and several natural secondary metabolizes. High industrial demands for
herbal products have over exploited its natural populations and have created a danger for its
genetic erosion. Itreproduces through seeds as well as successive vegetative growth of
rhizomes. Albeit, the direct sowing of rhizome segments is much popular than seed sowing
for early crop maturity, still it is expensive and labour intensive method of crop establishment
as the age of mother rhizome segments of H. spicatum affected the plant growth and
development.
An experiment was designed to determining the optimum age of rhizome segments for crop
production for which spherical rhizome segments of equal weight (50±2.5g, p>0.05) having
different age (1 to 5 year old segments) were sown The crop was harvested after 6 months
when it was in the complete senescence.
The 1-year-old rhizome segments revealed 100% sprouting of vegetative buds within
25.57±0.75 days, whereas, significant (p<0.05) and prolonged sprouting (68.77±3.66 to
96.00±1.44 days) with gradual decrease in sprouting (76.67±4.33 to 24.33±3.21%) was
observed for older rhizomes. The total aboveground and belowground fresh biomass
(220.33±12.91g and 124.7±12.62g, respectively) were also found significantly higher
(p<0.05) from 1-year-old mother rhizomes. In the ramets, the terminal 1-year-old rhizome
segment of H. spicatum revealed the best potential for successive growth and development.
Keywords: Hedychium spicatum, rhizome, age, ramet, sprouting, biomass
78
67. Seed Parameters and Regeneration Potential in Ficus palmate Forssk in
Nainital District of Kumaun
Jyotsna and Ashish Tewari
Department of Forestry and Environmental Science, D. S. B. Campus Kumaun University Nainital
Email: [email protected], [email protected]
The genus Ficus commonly known as fig belongs to the family Moraceae (Mathew et al.,
2011) and it is one of the largest plant genera, with more than 750 species distributed
worldwide (Corner, 1965). Ficus palmata is commonly known as ‘Bedu’. It occurs wild in
the Himalayan region between 1300 and 2000m elevational range. The present study was
carried out to study the vegetational parameters and regeneration of Ficus palmata in Nainital
District of Kumaun Himalaya across 2 selected sites. 9 permanent circular plots of 5.65cm
were placed randomly in the selected area. The total tree density at Site- I was 410indi/ha
with total basal area of 12.75m²/ha and at Site- II was 275 indi/ha withtotal basal area of
26.17m²/ha. Absence of seedling and sapling of Ficus palmata was conspicuous across all
sites. %. The fruit size varied between 179.35 to 127.57 mm² and the size of seed 0.819 to
0.211 mm².The seed germination in laboratory at room temperature varied between 31- 35.
The vegetational parameters indicate that natural regeneration of Ficus palmata is very low
because the fruit is edible for animal and human as a result fruit are collected or eaten before
maturation time.
Keywords: Germination, vegetational parameters, edible fruit
79
68. Effect of Silicon Solubilizer in Improving the Yield and Stress Tolerance in Rice
Genotypes
Babita Joshi
Department of Plant Physiology, G. B. P. U. A. &T., Pantnagar
Email: [email protected]
Rice is one of the most important food crop all over the world. Two- third of the global
population widely consume rice as a staple food. According to the global population growth
estimates indicates that by 2050 we will face the daunting prospect of feeding 9 billion
people and even over the next 20 years, demand for cereal grain is likely to increase by 50%.
To meet these burgeoning demands, improvements in cereal grain yield are required. Despite
of this large requirement the production of rice is not much increased due to declining in rate
of growth yields, depletion of natural resources, biotic and abiotic stress faced by the plant.
Among biotic stress insect infestation resulted in 10-15% of crop loss. Using nitrogen
fertilizer by farmer to increase the yield, the cost of production is increased but the decrease
in production observed due to enhance risk of disease especially blast and stem borer
infestation. To cope up with this situation there is need to enhance the production by
decreasing the yield losses through disease and pest infestation. Silicon is a beneficial
element for plant growth. It brings improvement in the rice yield cultivar without any genetic
improvement and also significantly help the plant in increasing the tolerance against biotic
and abiotic stress.
A field experiment was conducted at Norman E. Bourlog Crop Research Centre of G. B. Pant
University of Agriculture and Technology Pantnagar, India in June 2014. Six different
genotypes of rice with three replication each for control and treatment were taken for the
evaluation. The genotypes were DRRH-3, PA-6129, PA-6201, PA-6444, PHB-71 and BPT-
5204. Experiment was carried out in three separate split plot design. Soil application of
Silicon solubilizers applied at maximum tillering and panicle initiation stage.
Leaf area index, 1000- grain weight and economic yield, in almost all the rice genotypes were
significantly increased by silicon solubilizer treatment. The number of dead heart at panicle
initiation by stem borer larva has been reduced from 25-63% by silicon solubilizer treatment
with respect to control.
Using silicon solubilizer one would expect to raise the yield many fold, decrease insect
infestation in rice fulfilling the demand of overgrowing population.
80
69. Biology and Control of Three Problematic Weed Species of Winter Season
Prinsa Ramola and S. K. Guru
Department of Plant Physiology, College of Basic Sciences and Humanities. G. B. Pant University of
Agriculture and Technology Pantnagar-263145, U. S. Nagar, Uttarakhand, India
Email: [email protected]
Weeds are the most underestimated crop pests in tropical agriculture although they cause
maximum reduction in the yield of crops than other pests and diseases (Yaduraju, 2006).
They compete with crop for space, light, moisture and nutrients, and reduce the crop yield by
17 to 25%. (Shehzad et al., 2011). The investigation was conducted during the winter season
of 2014-15 to study the growth and development of three problematic weed species viz.,
Phalaris minor, Medicago denticulata and Anagallis arvensis and their competition with
crops. The field experiment was conducted at Norman E. Borlaug Crop Research Centre, G.
B. Pant University of Agriculture and Technology, Pantnagar. The bio-efficacy of different
herbicides for controlling these weeds species were also evaluated in another experiment. The
treatments consisted of clodinafop @ 60 g/ha, clodinafop+ mesosulfuron methyl @ 60 g+4.0
g/ha, sulfosulfuron @ 25g/ha, metribuzein @ 250 g/ha, pendimethalin @ 1 kg/ha and
trifluralin @ 1 kg/ha. This experiment was laid out in a randomized block design with three
replications.
It was observed that P. minor drastically affected the growth of both the crops under weedy situation.
Among the herbicides tested in the present study, the pre-emergnce herbicides pendimethalin and
trifluralin effectively controlled the germination of P. minor and A. arvensis. Among post emergence
herbicides, ready mix of clodinafop+MSM successfully controlled all the three weeds. Application of
clodinafop alone seems to be effective against only P. minor whereas, sulfosulfuron and metribuzin
were effective against the broad-leaved weeds. Phytotoxicity effect was observed in wheat in
trifluralin treatment whereas
81
70. Phytochemical Constituents and Pharmacological Activities of the Genus Thalictrum
(Ranunculaceae): A Review
Priyadarshini Chanyal, Deepshikha Joshi, Rajendra Prasad and Anand B. Melkani
Department of Chemistry, Kumaun University, Nainital, India
The genus Thalictrum (Ranunculaceae) is used as folklore medicine in China and India
amongst several countries in the world. It is commonly known as “meadow rue” and has been
a subject of several chemical and pharmacological studies. The genus grows wild in
Himalayan region of Uttarakhand, viz., T. elegans, T. platycarpum, T. cultratum, T.
chelidonii, T. neurocarpum, T. pauliflorum, T. virgatum, T. alpinum, T. punduanum, T.
saniculaeforme, T. javanicum, T. foliolosum and T. minus var foetidum.
The plant is used as traditional medicine, especially for the treatment of stomach discomforts,
snake bites, jaundice, rheumatism, skin rashes, to accelerate wound healing, to treat lung
cancer, cough, dysentery and to treat different microbial infections. Plants of this genus have
been reported to be used as sources of tonics, diuretics, antiseptics. More than 200 alkaloids
have been reported from the genus in which almost all belongs to isoquinoline group and
shows biological activities as hypotensive, antimicrobial and antitumor properties.
T. javanicum Blume could be used for the commercial production of drugs to treat dreadful
diseases caused by various pathogens, as it had potential antimicrobial activity. The solvent
extracts of different parts of T. rhyncocarpum have been reported as antimicrobial. Among
the volatile constituents of the essential oil of T. minus L., thymol (66.6%) and p-cymene
(13.0%) are reported as major components. The volatile extracts of T. reniforme Wall from
Ghanghariya (district Chamoli), Chopta (district Rudraprayag) and Drona village (district
Chamoli) have been reported terpenyl acetate (42.9%), epi-laurenene (40.2%) and α-bisabolol
(26.1%) as major components. In this review, we have explored the phytochemistry and
pharmacological activities of Thalictrum.
Keywords: Isoquinoline, pharmacological activities, phytochemical constituents,
Ranunculaceae, Thalictrum
82
71. Ethnomedicinally Important Wild Edible Plants of Nainital, Uttarakhand
Neha Chopra and Lalit M. Tewari
Department of Botany, D. S. B. Campus, Kumaun University, Nainital
Email: [email protected]
The present study was conducted in the Nainital region of Uttarakhand to document the
ethnomedicinal uses of wild edible plants. A total of 22 species belonging to 19 genera and
15 families was recorded. Plant species commonly used by local inhabitants for food,
medicine and in other fields of their lives were enumerated. A list of plant species along with
their family, habit, local names, plant parts used and mode of application was documented.
Fruit is the mostly used plant part (15) followed by leaves (11), flower (3), stem (2), berry
(2), whole plant (1), tuber (1), root (1), wood (1). These plant species are utilized by local
people against various ailments such as cuts and wounds, fever, joints pain, constipation,
diarrhea, eye disorders, skin problems, cough and cold, jaundice, asthma, bronchitis, etc. The
present study indicates conservation value of the area and the documentation will help the
foresters and policy makers to develop a suitable strategy and action plan for overall
development of the area as well as our nation.
Keywords: Ethnomedicine, wild edible, local people, Nainital
83
72. Impact of Alien Invasive Species on Soil physiochemical Characteristics in Sal
(Shorea robusta) and Chir pine (Pinus roxburghii) forest, Uttarakhand, India :A
Comparative study
Mamta Bharti and Lalit M.Tewari
Department of Botany, D. S. B. Campus, Kumaun University, Nainital
Lantana camara. L is an obnoxious exotic weed species (shrub) of global concern in the
conservation of biodiversity. The Invasive alien species (IAS) has profound impact on soil
ecosystem due constituting an advantage of allelopathic effects. The study concentrated on
analysis the impact of Lantana camara on physiochemical properties of soil in high and low
density Lantana invaded sites in Shorea robusta (Sal) forest and Pinus roxburghii (Chir-pine)
forest sites. Within each forest one highly invaded and one least invaded site was identified
and 15 plots were laid in each site. Soil were collected from three random places within these
sites and soil sample were collected from three layers i.e (0-10) (11-30) and (31-60) cm
depth. Two-way analysis of variance (ANOVA) result showed that their exist a remarkable
differences in the concentrations of soil macronutrient i.e soil pH, carbon (C), nitrogen (N),
phosphorous (P) and potassium (K) concentrations between highly invaded and least-
invaded sites and the nutrient availability were found to be higher in invaded sites. The soil of
these two forest were found to be sandy-loam and acidic in nature. Among these two forest
communities pine forest had higher macronutrient level in higher invaded sites than sal forest.
The overall result revealed that L. camara can improve the nutrient pool of soil and
increasing the soil macronutrient level for its successful establishment and growth.
Keywords: Invasive alien species, allelopathic effect, soil macronutrients, nutrient pool,
nutrient availability