ABSTRACT

Validation of Spectrophotometric UV-Vis for Determination ofVitamin B1 and Caffeine in Tablet Simulation Mixture

Second order derivative Spectrophotometry has been used for determinationvitamin B1 and caffeine. The selected wavelength was 236.0 nm and 224.0 nm. Thedetermination provides responses for a number of chemical entities that may bedistinguished from each other. Several parameters can be used for basicevaluation of linier calibration, such as correlation coefficient (r), p, and Vxo value.The determination of vitamin B1 had coefficient of regresion y = 1.55x10-4 x+11.52x10-4 (r = 0.9991; p= 0.0019, p<0.01) and Vxo=2.44%. And caffeine hadcoefficient of regression y = 6.54x10-4 x +1.23x10-4 (r = 0.9999; p = 0.0102, p<0.05)and Vxo= 0.42%. Coefficient of Variation was used as parameter for equipmentprecision’s were 0.75% for vitamin B1 and 0.94% for caffeine. It is acceptable CVbecause less than 2%. The composition of the mixture vitamin B1 and caffeine are4:1, 3:1, 1:1, 1:4, and 1:3. The result of average recovery is 99.64% and 99.53% forvitamin B1 and caffeine. The acceptance criterion for recovery is 98% - 102%. Forsimulation tablet and “X” tablet had error percentage 3.71% and 2.37% for vitaminB1, caffeine 6.31% and 2.45%. it was not complied for the requirements.

Keyword : Spectrophotometric UV-Vis, Vitamin B1, Caffeine