Gluten detection kits

Emport, LLC for Biomedal SL

Simple ● Specific ● Sensitive

What is Gluten detection?

Different gluten detection kits use different antibodies to identify components of gluten.However, most components of gluten are actually safe for people with celiac disease!Research indicates that a only very small fraction is toxic for celiacs: the 33-mer peptide

of the a-gliadin molecule.

The G12 antibody in all GlutenTox kits was specifically developed to detect this toxicpeptide. G12 searches for the QPQLPY sequence, found only in the 33-mer peptide.

Because of this specificity, G12 is a superior choice for fermented, hydrolyzed, or heat-treated foods with complicated molecular structures.

Antibody 33-merpeptide

“Gluten” refers to the storageproteins in certain grains. Gluten iscomposed of several different typesof protein, for example wheatcontains several different types of

gliadins and glutenins.

G12, Celiac Disease and OatsQ: Are oats gluten-free?

A: Sometimes.

Oats are often subject to cross contamination (in the field and/or during processing).

However, just because oats have been grown andprocessed carefully does not mean they are gluten-free.A 2010 study showed that not all oats are created equal.Different strains of oat have different avenin structures, andonly some are toxic to celiacs.

The study used the G12 antibody to review several strains ofoat:

• 3 distinct groups of oat were identified: no, low andhigh toxicity strains.

• The strains were introduced to cells from celiacpatients, and the cells responded appropriately: theyonly reacted to the toxic oat strains.

Accordingly, G12 is the best choice for any companyworking with gluten-free oats.

P

V

RV

P

Q

MV

L

Q

P

Q

N

P

S

Q

Q

Q

P

Q

E

Q

V

F

P

S

Q

Q

P

Y

P

L

V

Q

Q

QQ

F

P

G

Q

QQ

P

F

P

P

Q

Q

P

YP

QP

Q

P

R

P

Q

Q

P

YP

Q

S

Q

P

Q

Y

S

Q

P

Q

Q

P

IS Q

Q

Q

Q

Q

Q

Q

Q

Q

Q

Q

Q

K

Q

Q

Q

Q

Q

Q

Q

QI

LQ

Q

I

L

Q

Q

QQ

Q

R

QG

Q

L

C

G

L

E

Q

D

H

S

Q

V

Q

N

S

P

I

L

V

S

S

S

Q

Q

P

V

Q

R

E

I

V

I

Q

T

Q

Q

Q

F

N

L

P

L

A

V

Y

L

A

P

E

LA

C

Q

Y

L

Q

C

V

Y

G

Q

P

A

C

I

T

I

C

V LQ

Q

S

P

S

Q

L

M

T

A

N

P

Q

V H Q Q

F

S

F

T

N

C

P

Y

E

A

HQ

Q

P

Q

G

Q

V

Y

V

R

L

Q

I

A

Q

Q

L

Q

Q

P

Y

P

G

G

W

S

H

I

Q

Q

S

P

Q

S

IP

I

F

Q

RN

I

Q

Q

Q

Q

Q

Q PQ

L

P

Y

P

Q

P

Q

LP

YP

QP

Q

P

F

P

Y

P

L

Q

P

Q

P

F

P

Q

L

Q

L

a-gliadin

molecule33-mer peptide

G12

binding

The G12 antibody recognizes the 33-mer peptide, which is present

in the a- gliadin protein and which triggers celiac disease

Uses ofThe GlutenTox line of products is an easy, cost-effective way to:

Check surfaces in cases of accidental cross-contamination

Verify that sauces/soups/large-batch items are safe for gluten-free diners

Confirm that incoming ingredients are safe (A study published by the

American Dietetic Association found that 30% of inherently gluten-free

grains found in the supermarket had more than 20ppm gluten (corn flour,

rice, millet, etc);

Spot-checking dishes to ensure that staff is appropriately trained in

allergen-free preparation

Spot-check and quantify incoming ingredients, batches of product,

surfaces, etc.

We want using GlutenTox to be as easy as possible, and will work with you todevelop a program that meets your needs.

Biomedal’s GlutenTox Products Lateral flow devices (immunochromatographic strips)

Contain the G12 anti-gliadin antibody detecting the

immunotoxic 33-mer peptide

Detect gluten in wheat, barley, rye and also oat

No cross-reactivity with soy, corn, rice, quinoa etc.

Test gluten in solid food and liquids

Trace gluten contamination (in food or surfaces)

Long shelf life: 18 months from manufacture

Easy storage and shipping at room temperature (35°F - 85°F)

Certification by AESAN (Spanish Agency for Food Safety and

Nutrition, Spanish Ministry of Heath and Social Politics) and

the FAPAS Proficiency Test

Biomedal holds the ISO:9001:2008 (EC-3105/07) and

ISO:17025:2005 (925/LE1568).

Biomedal’s GlutenTox Products

• User-friendly gluten detection kit with individually-wrapped tests

• Qualitative testing for 20 ppm or 5 ppm

• For food and drinks

• 10-20 minutes

• All equipment included

• Easy to use gluten detection kit for commercial kitchens

• Semi-quantitative testing at 5 ppm, 10 ppm, 20 ppm, or 40 ppm

• For food, drinks and surface testing

• 10-20 minutes

• All equipment included

• Semi-professional gluten detection kit for food manufacturers

• Semi-quantitative testing at any gluten threshold (≥3ppm)

• For food, drinks and surface testing

• 1 hour

• Some basic lab equipment required

For celiac

patients to avoid

accidental gluten

ingestion

Material provided (no other required, just the sample):

Spoon Solution 1 Solution 2 Pipettes Test

Instructions Available on

For food manufacturers and commercial kitchens to avoid accidental gluten

contamination. Suitable for food, liquid

and surface testing

Material provided (no other material required but the sample):

Spoon Solution 1 Solution 2 Pipettes 25 Tests

Threshold (drops of extraction solution needed):

8 drops = 5 ppm4 drops = 10 ppm2 drops = 20 ppm

1 drop = 40 ppm

Instructions: Food and Drinks

How to use it: Surface Testing

The most sensitive

semi-quantitative rapid

test available

Necessary materials (but non-provided):

Scale

Micropipettes

and tips

14 ml

Tubes

Centrifuge or Filters

Eppendorf

Tubes

Instructions

Extraction

Analysis

How to Surface Swab

Test will indicate positive or negative result (cannot be extrapolated in gluten value).

A negative result means that 1 kilo of dough, kneaded on this surface will have less than 0.01 ppm of gluten.

Reader

• Use a 1:10 dilution ratio with

GlutenTox Sticks

• Insert test strip into Reader

• A precise PPM count appears on

the screen in <1 minute

• Data is stored on the Reader and

easily transferable to any

computer via USB

Quantitative analysis with

:a precise PPM

measurement in seconds!

Ordering Info

Emport is happy to provide phone or video training on any GlutenTox

test kit. Just ask!

Ask us about setting up an automatic monthly shipment of

tests or about alternate quantity needs.

can be ordered online in increments of 2, 5 or

10 tests, or in bulk by contacting Emport.

can be ordered by contacting Emport.

Increments of 25 only.&

Q: Are test results valid sooner than 10 minutes / after more than 10 minutes?

A: If the sample has a high concentration of gluten, a pink line may appear in

only a few minutes. This is a valid result. However, if no pink line appears you

must wait the full 10 minutes to declare a truly gluten-free sample.

Test results may shift if left out for too long (positive results fade in intensity and

negative results sometimes develop a faint pink positive line), so it’s important

to check promptly at 10 minutes.

Q: Can you show me some studies?

A: • Gut journal study showing how G12 works with oat

• Stanford University study explaining the 33-mer peptide

• Stanford University, University of Seville and Biomedal study

explaining the G12 and related A1 antibodies

• American Dietetic Association study of inherently gluten-free grains

FAQs

FAQsQ: Why is the kit not recommended for chocolate?

A: Gluten from chocolate or cocoa is very hard to extract because of the

phenols present in this matrix: they can chelate gluten. For this reason the level of

gluten can be slightly underestimated. However, if you get a positive result with

chocolate, it definitely does contain gluten and should not be consumed by

celiac disease patients.

Chocolate

is a Sticks kit with a special extraction

buffer specifically developed to test

cacao or chocolate.

A: Yes, the G12 antibody specifically recognizes the

fragment that is toxic to celiac patients and can

therefore assess toxicity even if the food is

fermented, hydrolyzed or heat processed.

Q: Can the kit be used with fermented, hydrolyzed or baked goods ?

FAQsQ: Why is it important to shake the sample vigorously in the extraction stage?

A: This stage is crucial because it breaks apart the sample so that the gluten

molecules can be isolated / detected. The first step is to grind and homogenize

the sample as much as possible. The second step is to shake it, which continues

the grinding/breaking-down process.

Q: What do I do with the test once I’ve used it?

A: All elements of all GlutenTox tests can be disposed of in

ordinary trash, or recycled where appropriate.

If you need to stop briefly during the 2 minutes of

shaking, it’s OK. Just make sure the total amount of

shaking time is at least 2 minutes.

FAQsQ: Do I need to purchase separate swabs for surface testing?

A: Nope! The test strip is all you need. You’ll notice that when the strip is face-

up, one end has a fuzzy, cotton texture. This end is what gets rubbed directly

onto the surface you are testing, and it is also what gets inserted into the

dilution solution.

Q: Can I use GlutenTox Home to surface test?

A: We recommend using GlutenTox Pro or GlutenTox Sticks, however in a

pinch you can modify GlutenTox Home to surface test. Simply pry open the

plastic casing with a thin blade and remove the test strip. You can then treat

it like any other surface test.

One thing to be careful of: always surface test

using clean hands (or a glove), to ensure thatyou do not accidentally contaminate the strip.

More Info On GlutenTox:

http://www.glutentox.com

More Info On Biomedal:

http://www.biomedal.com

Contact Info:

Links and Contact Info

emilyk@emportllc.com 412.447.1888

Thank you !