Abacus Diagnostics – Dramatically Improving Crime Scene ... · Cells were lysed for I h at 4 QC...

8

Transcript of Abacus Diagnostics – Dramatically Improving Crime Scene ... · Cells were lysed for I h at 4 QC...

Page 1: Abacus Diagnostics – Dramatically Improving Crime Scene ... · Cells were lysed for I h at 4 QC in the rotator. Samples were centrifuged for 10 min at 14.000 rpm and the pellets
Page 2: Abacus Diagnostics – Dramatically Improving Crime Scene ... · Cells were lysed for I h at 4 QC in the rotator. Samples were centrifuged for 10 min at 14.000 rpm and the pellets
Page 3: Abacus Diagnostics – Dramatically Improving Crime Scene ... · Cells were lysed for I h at 4 QC in the rotator. Samples were centrifuged for 10 min at 14.000 rpm and the pellets
Page 4: Abacus Diagnostics – Dramatically Improving Crime Scene ... · Cells were lysed for I h at 4 QC in the rotator. Samples were centrifuged for 10 min at 14.000 rpm and the pellets
Page 5: Abacus Diagnostics – Dramatically Improving Crime Scene ... · Cells were lysed for I h at 4 QC in the rotator. Samples were centrifuged for 10 min at 14.000 rpm and the pellets
Page 6: Abacus Diagnostics – Dramatically Improving Crime Scene ... · Cells were lysed for I h at 4 QC in the rotator. Samples were centrifuged for 10 min at 14.000 rpm and the pellets
Page 7: Abacus Diagnostics – Dramatically Improving Crime Scene ... · Cells were lysed for I h at 4 QC in the rotator. Samples were centrifuged for 10 min at 14.000 rpm and the pellets
Page 8: Abacus Diagnostics – Dramatically Improving Crime Scene ... · Cells were lysed for I h at 4 QC in the rotator. Samples were centrifuged for 10 min at 14.000 rpm and the pellets

Supporting Information - PNAS...Immunoblotting.T-Rex HeLa cells were transfected with rsKame-Lk-Drp1 and induced for 3.5 h with 0.75 μM doxycycline. Cells were lysed with M-PER (mammalian

Supporting Information - PNAS...Immunoblotting.T-Rex HeLa cells were transfected with rsKame-Lk-Drp1 and induced for 3.5 h with 0.75 μM doxycycline. Cells were lysed with M-PER (mammalian

CENTRIFUGED REINFORCED CONCRETE ELECTRICAL POLESgpzagorje.hr/.../centrifuged_reinforced_concrete_electrical_poles_1... · ROUND CENTRIFUGED REINFORCED CONCRETE ELECTRICAL POLES .

CENTRIFUGED REINFORCED CONCRETE ELECTRICAL POLESgpzagorje.hr/.../centrifuged_reinforced_concrete_electrical_poles_1... · ROUND CENTRIFUGED REINFORCED CONCRETE ELECTRICAL POLES .

Improved Chemistry for SingleCell Transcriptome Analysis...Methods and References In the experiments described here, K562 cells (human leukemia cell line) were captured, lysed, and

Improved Chemistry for SingleCell Transcriptome Analysis...Methods and References In the experiments described here, K562 cells (human leukemia cell line) were captured, lysed, and

Numerical modelling of a centrifuged embankment on soft … modelling of a centrifuged... · Numerical modelling of a centrifuged embankment on soft clay ... embankments on soft clays.

Numerical modelling of a centrifuged embankment on soft … modelling of a centrifuged... · Numerical modelling of a centrifuged embankment on soft clay ... embankments on soft clays.

Oncostatin M drives intestinal inflammation and predicts ... · Fiona.powrie@kennedy.ox.ac.uk Nature Medicine, ... (Mitutoyo, UK), ... Cells were centrifuged for 15 minutes at 10°C

Oncostatin M drives intestinal inflammation and predicts ... · [email protected] Nature Medicine, ... (Mitutoyo, UK), ... Cells were centrifuged for 15 minutes at 10°C

Metabolomic and Proteomics Solutions for Integrated Biology · Proteomics: Samples were lysed using glass beads with vortexing and digested using the modified FASP protocol ASMS 2014

Metabolomic and Proteomics Solutions for Integrated Biology · Proteomics: Samples were lysed using glass beads with vortexing and digested using the modified FASP protocol ASMS 2014

CHEMICAL RESEARCH, 8i ENGINEERING CENTER · The mixture was centrifuged (1000 x g, 10 min, 40 C) and the supernatant stored on ice. Pellets were rehcmogenized, centrifuged as before,

CHEMICAL RESEARCH, 8i ENGINEERING CENTER · The mixture was centrifuged (1000 x g, 10 min, 40 C) and the supernatant stored on ice. Pellets were rehcmogenized, centrifuged as before,

Genetically-Engineered Bacteriophage for Diagnosis of ...2012.igem.org/files/poster/Virginia.pdfCells containing a heterogeneous mixture of BBa_K732001 and BBa_K732002 were lysed by

Genetically-Engineered Bacteriophage for Diagnosis of ...2012.igem.org/files/poster/Virginia.pdfCells containing a heterogeneous mixture of BBa_K732001 and BBa_K732002 were lysed by

Enumeration Anaerobic Bacterial Microflora of …aem.asm.org/content/54/9/2155.full.pdfbasic physiological studies of the gastrointestinal system. ... samples were centrifuged and

Enumeration Anaerobic Bacterial Microflora of …aem.asm.org/content/54/9/2155.full.pdfbasic physiological studies of the gastrointestinal system. ... samples were centrifuged and

CBP MEDIATES SEQUENTIAL ENZYMATIC DOWN … · cells were lysed with BugBuster (Novagen) as described by the manufacturer, and ... were raised in rabbits according to the protocol

CBP MEDIATES SEQUENTIAL ENZYMATIC DOWN … · cells were lysed with BugBuster (Novagen) as described by the manufacturer, and ... were raised in rabbits according to the protocol

Response to Inoculation Hemagglutinin- Hemaggiutinin ......sue culture tubes to obtain sufficient volume for volunteer inoculations. These pools were centrifuged, submitted to treatment

Response to Inoculation Hemagglutinin- Hemaggiutinin ......sue culture tubes to obtain sufficient volume for volunteer inoculations. These pools were centrifuged, submitted to treatment

til) 10Cc/) - Journal of Bacteriologyjb.asm.org/content/85/3/499.full.pdf · Plasmolyzed cells were fixed in 10Cc/) formalin for 1 hr at 24 C. The cells were then centrifuged, and

til) 10Cc/) - Journal of Bacteriologyjb.asm.org/content/85/3/499.full.pdf · Plasmolyzed cells were fixed in 10Cc/) formalin for 1 hr at 24 C. The cells were then centrifuged, and

Supplemental Methods Expression and purification of ... · and were lysed in 300 μL Bugbuster (Novagen) containing protease inhibitor tablets (Roche) and benzonase (Novagen). The

Supplemental Methods Expression and purification of ... · and were lysed in 300 μL Bugbuster (Novagen) containing protease inhibitor tablets (Roche) and benzonase (Novagen). The

Research Paper CDK5RAP3 Inhibits the Translocation of MCM6 ... · Immunoprecipitation Cells were washed with icecold PBS and lysed - in Tris-buffered saline (pH 7.4) containing 50

Research Paper CDK5RAP3 Inhibits the Translocation of MCM6 ... · Immunoprecipitation Cells were washed with icecold PBS and lysed - in Tris-buffered saline (pH 7.4) containing 50

ContradictoryFunctions(Activation/Termination)of ... · HMC-1 and Raw 264.7 cells were stimulated with precursor and mature IL-33 at various time points. Cells were lysed with kinase

ContradictoryFunctions(Activation/Termination)of ... · HMC-1 and Raw 264.7 cells were stimulated with precursor and mature IL-33 at various time points. Cells were lysed with kinase

Cellular/Molecular Anoxia ... · drugs/anoxia, slices were quickly lysed in RIPA lysis buffer (Invitrogen) usingamini-Douncehomogenizer.Westernblotanalysisofhippocam-pal–neocortical

Cellular/Molecular Anoxia ... · drugs/anoxia, slices were quickly lysed in RIPA lysis buffer (Invitrogen) usingamini-Douncehomogenizer.Westernblotanalysisofhippocam-pal–neocortical

Glucose-Based Peritoneal Dialysis Fluids …Western blotting. Treated/untreated subconfluent cells were lysed in lysis buffer (Cell Signaling Technology Inc., Danvers, MA). Protein

Glucose-Based Peritoneal Dialysis Fluids …Western blotting. Treated/untreated subconfluent cells were lysed in lysis buffer (Cell Signaling Technology Inc., Danvers, MA). Protein

Chemistry and Physics of Lipids - lipid.phys.cmu.edulipid.phys.cmu.edu/papers18/SPMcpl.pdf · To clarify the above ... lipids were mildly centrifuged using a desk-top centrifuge in

Chemistry and Physics of Lipids - lipid.phys.cmu.edulipid.phys.cmu.edu/papers18/SPMcpl.pdf · To clarify the above ... lipids were mildly centrifuged using a desk-top centrifuge in

De-novo purine biosynthesis is a major driver of chemoresistance … · 2020. 3. 13. · Immunoprecipitation: Following trypsinization, cells were lysed using mPER buffer, and protein

De-novo purine biosynthesis is a major driver of chemoresistance … · 2020. 3. 13. · Immunoprecipitation: Following trypsinization, cells were lysed using mPER buffer, and protein

Angiotensin II up-regulates soluble epoxide hydrolase in ...or 100 nM Ang II for different periods of time. Cells were lysed, and proteins were resolved by 10% SDS/PAGE, transferred

Angiotensin II up-regulates soluble epoxide hydrolase in ...or 100 nM Ang II for different periods of time. Cells were lysed, and proteins were resolved by 10% SDS/PAGE, transferred