Aakanksha Vatsal, Smita Zinjarde and Ameeta Ravi Kumar · 2015-10-30 · Aakanksha Vatsal, Smita...
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BIOTECHNOLOGY-2011 312 21-23 March 2011 World Congress on Biotechnology T he extensive use of halogenated aromatics as organic solvents, chemical additives has led to their widespread release into the environment. e harmful haloaromatics, like bromobenzene, cause hepatotoxicity in man and animals. ere are very few reports available for the detoxification and degradative products of such haloaromatic compounds. Yarrowia lipolytica NCIM 3589, a marine isolate has the ability to degrade halogenated compounds by the dehalogenation mechanism. Dehalogenases or halidohydrolases are hydrolytic enzymes which cleave the carbon-halogen bond(s) in halogenated organics bringing about a decrease in recalcitrance of the xenobiotic compounds and making these compounds less toxic. When grown on bromobenzene, Y. lipolytica showed an initial increase in growth rate at lower substrate concentrations with maximal growth being obtained on 2% v/v concentration of the compound (1.88× 10 7 ) in 72 h. e extracellular dehalogenase activity was found to be inducible only in presence of the bromoaromatic as the sole carbon source. Y. lipolytica exhibited maximal extracellular enzyme activity on 1% (v/v) of bromobenzene in 72 h (8.08 U ml -1 ), indicating that the enzyme was required for growth of the yeast. e degradation of bromobenzene by Y. lipolytica was studied and the products were analyzed. GC analysis of bromobenzene degradation was done and carbon dioxide release was detected therby indicating detoxification of bromobenzene. We thus propose a probable route for metabolism of the compound. Bromobenzene degradation by a tropical marine yeast, Yarrowia lipolytica Aakanksha Vatsal, Smita Zinjarde and Ameeta Ravi Kumar University of Pune, India Biography Aakanksha Vatsal has completed her post graduation from the University of Pune. Currently she is a senior research fellow at Institute of Bioinformatics and Biotechnology and is pursuing her PhD work since May 2007 under the guidance of Dr. Ameeta Ravi Kumar. doi:10.4172/2157-7048.1000001
Transcript of Aakanksha Vatsal, Smita Zinjarde and Ameeta Ravi Kumar · 2015-10-30 · Aakanksha Vatsal, Smita...
BIOTECHNOLOGY-2011
312
21-2
3 M
arch
2011
World Congress on
Biotechnology
The extensive use of halogenated aromatics as organic solvents, chemical additives has led to
their widespread release into the environment. The harmful haloaromatics, like bromobenzene, cause hepatotoxicity in man and animals. There are very few reports available for the detoxification and degradative products of such haloaromatic compounds. Yarrowia lipolytica NCIM 3589, a marine isolate has the ability to degrade halogenated compounds by the dehalogenation mechanism. Dehalogenases or halidohydrolases are hydrolytic enzymes which cleave the carbon-halogen bond(s) in halogenated organics bringing about a decrease in recalcitrance of the xenobiotic compounds and making these compounds less toxic. When grown on bromobenzene, Y. lipolytica showed an initial increase
in growth rate at lower substrate concentrations with maximal growth being obtained on 2% v/v concentration of the compound (1.88× 107) in 72 h. The extracellular dehalogenase activity was found to be inducible only in presence of the bromoaromatic as the sole carbon source. Y. lipolytica exhibited maximal extracellular enzyme activity on 1% (v/v) of bromobenzene in 72 h (8.08 U ml-1), indicating that the enzyme was required for growth of the yeast. The degradation of bromobenzene by Y. lipolytica was studied and the products were analyzed. GC analysis of bromobenzene degradation was done and carbon dioxide release was detected therby indicating detoxification of bromobenzene. We thus propose a probable route for metabolism of the compound.
Bromobenzene degradation by a tropical marine yeast, Yarrowia lipolyticaAakanksha Vatsal, Smita Zinjarde and Ameeta Ravi KumarUniversity of Pune, India
BiographyAakanksha Vatsal has completed her post graduation from the University of Pune.
Currently she is a senior research fellow at Institute of Bioinformatics and Biotechnology and is pursuing her PhD work since May 2007 under the guidance of Dr. Ameeta Ravi Kumar.
doi:10.4172/2157-7048.1000001
