A1R-Ti-‚¤ƒ³ƒ†ƒ³‚·ƒ©‚¤ƒˆ...

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  • A1(v.3.203.22)

  • Operating Manual A1

    2

    1

    2

    3

    4

    Ti-E 4.1

    Ti-E 4.2

    Hg 4.3

    XY 4.4

    A1 4.5

    5

    6

    Z 7

    8

    Slice View Projection 9

    10

    Time Measurement 11

    12

    ROI CROP 13

    14

    24

    26

    27

    ND2 28

    ND2 29

    30

    31

  • Operating Manual A1

    3

    1. A1 A1

  • Operating Manual A1

    4

    A1TiEPFS

  • Operating Manual A1

    5

    2.

    2.1

  • Operating Manual A1

    6

    2.2

    Key off 90

    2.3

    on

    2.4

    2.5 NISElements

    NISElements

    Key

    PAWER

  • Operating Manual A1

    7

    3

    3.1. NIS-Elements

    3.2.

    3.3.

    3.4.

    3.5.

  • Operating Manual A1

    8

    4

    4.1. Ti Ti-RCP

    1)

    Ti-RCP

    PFS on/off

    .

    .

    .

    ON/OFF

    .

    .

    .

    .

    .

    .

    .

    DAPI FITC TIRTC DIC A1

    .

  • Operating Manual A1

    9

    2)

    D NCB

    ND GIF

  • Operating Manual A1

    10

    4.2. Ti

    1)

    Ti

    2)

    IN

    PFS ON/OFF PFS

    PFS ON/OFF

    PFS

    . Ti-E

    PFS ON/OFF

  • Operating Manual A1

    11

    5

    5.1 NISElements

    NISElements

    5.2

    Ti

    ND

    5.3 A1

    Ti A1

    5.4 Laser Inter Locked

    A1

    5.5.

    Galvano

    5.6 Optical Path

    Setting Setting

    A1

  • Operating Manual A1

    12

    Auto

    Optical Path

    OKOptical

    Path

    5.7

    /

    TD IN TD

    Pinhole

    Home

    Live

    HV

    Offset 0

    Color Overexposured

    LUTs

    Auto Scale

    AllLUTs

    Live ON/OFF

    6

    6

    6

    6

    Color Overexposured

    Auto Scale All

  • Operating Manual A1

    13

    Auto Gain HV

    AG

    Auto Gain NGHV

    Auto Gain

    5.8

    512512

    Average Average Count 24816

    XY

    5.9

    File->Save As

    nd2

    LUT

    LUT

    3

    4 1

  • Operating Manual A1

    14

    7

    7

    7

    8

    8

    7

    8

    7

  • Operating Manual A1

    15

    2

    6

    6.1 16

    6.2

    Ch series Line /

    TD IN TD

    Pinhole

    Home

    Live

    HV

    Auto Gain AG

    Split Components

    Offset 0

    Color Overexposured

    LUTs

    Auto

    Scale AllLUTs

    Live ON/OFF

    Split Components

    Color Overexposured

    Auto Scale All

  • Operating Manual A1

    16

  • Operating Manual A1

    17

    6.3

    512512

    Average Average Count 24816

    XY

    6.4

    File->Save As

    nd2

    2

    2

    2

    3

    3

    2

    3

    2

  • Operating Manual A1

    18

    7 Z

    7.1 17

    7.2 Z

    XYZ Capture ZSeries

    Defined topbottom

    Reset

    Live

    Top Top

    Live

    Bottom Bottom

    Step

    Save to File

    nd2

    7.3 Z

    512

    512

    Average Average

    Run now Z

    2

    2

    2

    2

    2

    2

    2

    2

    3

    2

    2

  • Operating Manual A1

    19

    8

    8.1

    Z

    Show Volume View

    3

    8.2 3 1

    3

    Ctrl+

    Edit->Create View Snapshot

    File->Save As jpgtiffbmp

    8.3

    3 Show

    Movie Maker

    Volume

    Presets

    Settings

    Movie Duration

    Frames per Second

    OK

    Frames per Secondfps

    1

    310fps

    Create Movie

    FileSave As

    avi

    avi

    No Compression

    1

    3

    3.

    3.

    3.

  • Operating Manual A1

    20

    Use Original AcqTime

    9 Slice View Projection

    9.1 Slice View Projection

    Z

    Show Slices View

    XZYZ

    Show Maximum Intensity Projection

    1

    1

  • Operating Manual A1

    21

    9.2

    Edit->Create View Snapshot

    File->Save As jpgtiffbmp

    10

    10.1 17

    10.2

    XY Time Capture Time lapse

    IntervalDuration

    No Delay

    Phase Phase

    Phase

    Save to File

    nd2

    Perfome Time

    Measurement

    10.3

    512512

    Average

    Average

    Run now

    2

    2 2

    2

    3

    2

  • Operating Manual A1

    22

    11 Time Measurement

    11.1

    11.2 Time Measurement

    Time Measurement

    ->Analysis Controls ->Time Measurement

    11.3 ROI Region Of Interest

    Define Define ROI

    ROI

    Finish

    3

  • Operating Manual A1

    23

    3 3

  • Operating Manual A1

    24

    11.4

    Measure

    Multi ROIs ROI

    1

    Multi DyesALLCustom

    ROI 1

    Export Data Export

    Edit->Options->Date export->Global Settings->Export text files into

    folder

    11.5 Time Measurement

    4

    4 4

    ROI

    ROI SHIFT

    2

    2

  • Operating Manual A1

    25

  • Operating Manual A1

    26

    12

    12.1 17

    12.2

    Scan Area

    Zoom

    12.3

    Rotation

    90 90

    Scan Area

    ->Acquisition Controls ->Scan Area

    4

    2

  • Operating Manual A1

    27

    12.4 Live

    13 ROI CROP

    13.1 17

    13.2

    ROI ROI

    Scan Area Edit

    ROI

    CROP

    Scan Area Crop

    Scan Area

    ->Acquisition Controls ->Scan Area

    3

    2

  • Operating Manual A1

    28

    13.3 Live

    14

    14.1 17

    14.2

    Simple ROI

    Editor

    ROI

    ROI 3

    ROI

    ROI ->Use as

    Stimulation ROI Stimulation

    Group

    ROI

    14.3

    Camera Setting Photo Activation

    1Stimulation Group1

    Scan Speed 1

    1 Sec/Frame 1 1

    Stimulation Group23

    2

    2

  • Operating Manual A1

    29

    14.4

    Camera Setting Photo Activation

    Simultaneous Stimulation

    Acq/Stim

    ROIs Stimulation Group

    Interval or

    Duration or

    Loops

    Loops or

    Save to File

    nd2

    Run now

    4

    2

    3

    3

    3

    3

    4

    4

  • Operating Manual A1

    30

    A1TiEPFS

  • Operating Manual A1

    31

    24

    241 16

    24.2

    Resonant

    24.3

    30frame/second

    Scan Area

    Scan Area

    ->Acquisition Controls ->Scan Area

    51232 420fps 4

  • Operating Manual A1

    32

    /

    D IN TD

    Pinhole

    Home

    Live

    HV

    Auto Gain HV

    AG

    Auto Gain NGHV

    Auto Gain

    Offset 0

    Color Overexposured

    LUTs

    Auto Scale

    AllLUTs

    Live ON/OFF

    Color Overexposured

    Auto Scale All

    1

    3

  • Operating Manual A1

    33

    24.4

    Average Average

    Average 1

    Interval No Delay

    Interval 100msec

    Run now

  • Operating Manual A1

    34

    26

    26.1 13

    26.2

    Simple ROI

    Editor

    ROI

    ROI 3

    ROI

    ROI Use as Stimulation ROI Stimulation Group

    ROI

    26.3

    Camera Setting Photo Activation

    1Stimulation Group1

    403nm

    Scan Speed 1

    1 Sec/Frame 1 1

    Stimulation Group23

    2

    2

  • Operating Manual A1

    35

    26

    .4

    Camera Setting Photo Activation

    Simultaneous Stimulation

    Wait

    Interval

    ROIs Stimulation Group

    Duration

    Loops

    Loops

    Duration

    Loops

    Loops

    Save to File

    nd2

    Run now

    4

    2

    3

    3

    3

    3

  • Operating Manual A1

    36

    4

    4

    4

    4

    4

  • Operating Manual A1

    37

    27

    271

    Custom

    Custom

    Split Components

    27.2

    Show Tiled View

    27.3

    Show Scale Scale

    Properties->Scale ->Automatically adjust size size

    27.4

    Display ND Information -> Acq

    Time

    AcqTime ND Info Properties

    AcqTime

    AcqTime File -> Save As avi

  • Operating Manual A1

    38

    27.5

    Auto Scal All

    Black White Gamma

    Reset LUTs

    Color Overexposured

    Color Overexposured

    Auto Scale All

  • Operating Manual A1

    39